BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,503,111	A→G	5.8%	I234I (ATT→ATC)	norB_4 ←	Quinolone resistance protein NorB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,503,111	0	A	G	5.8%	342.7 / 7.2	115	I234I (ATT→ATC)	norB_4	Quinolone resistance protein NorB
Reads supporting (aligned to +/- strand): ref base A (48/59); new base G (4/3); total (53/62)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

ATTTAACAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCAGGTGT  >  USA300TCH1516_ALE/1502973‑1503251

aTTTAACAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGtaat                                                                                                                                             <  1:679390/141‑1 (MQ=255)

   tAACAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑aaa                                                                                                                                         <  2:45279/141‑1 (MQ=255)

   tAACAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑aaa                                                                                                                                         <  4:354146/141‑1 (MQ=255)

    aaCAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑aaaa                                                                                                                                        >  2:547207/1‑141 (MQ=255)

     aCAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑aaaaa                                                                                                                                       <  1:896370/141‑1 (MQ=255)

     aCAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑aaaaa                                                                                                                                       <  3:437494/141‑1 (MQ=255)

      cAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAaag                                                                                                                                      <  1:334564/141‑1 (MQ=255)

        aaaaGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAaagaa                                                                                                                                    >  1:366249/1‑141 (MQ=255)

          aaGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AACAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGt                                                                                                                                  <  2:935301/141‑1 (MQ=255)

          aaGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGt                                                                                                                                  <  1:4606/141‑1 (MQ=255)

           aGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTg                                                                                                                                 >  1:1015964/1‑141 (MQ=255)

              ttGAAGCTGTTGCACCTGTGTACGCTTTATTTTTAACTAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAgg                                                                                                                              >  1:994288/1‑141 (MQ=255)

              ttGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCATGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAgg                                                                                                                              >  2:365353/1‑141 (MQ=255)

                gAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTCTAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGtt                                                                                                                            >  1:762151/1‑141 (MQ=255)

                gAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGtt                                                                                                                            >  4:377713/1‑141 (MQ=255)

                gAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGtt                                                                                                                            >  1:536078/1‑141 (MQ=255)

                gAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGtt                                                                                                                            >  4:338224/1‑141 (MQ=255)

                gAAGCTGTTGCACCTGTATAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAGCTATAAATAAACT‑AAAAGATCCAATTGCAATTGCTAATAAAGTGAT‑AAAAAGAAGTGAGGtt                                                                                                                            >  2:571825/1‑141 (MQ=255)

                     tGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACAcc                                                                                                                       <  2:628125/141‑1 (MQ=255)

                     tGTTGAACCTGTGTAAGCTATATTTTTAAATAATTTAACCTAGATACAAAGCTTCATAAAACGCTTTTCAAGAACTATAAATAAACT‑ACACGAACAAATTCAAATAGATAATAAAGTCAT‑AAAAAGAAGTGAggatccaac                                                                                                                       >  2:48099/1‑134 (MQ=255)

                         gCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGCTTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAAt                                                                                                                   >  2:84332/1‑141 (MQ=255)

                          cACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAAtt                                                                                                                  >  1:966153/1‑141 (MQ=255)

                          aaCCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAAtt                                                                                                                  <  2:456981/140‑1 (MQ=255)

                           aCCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTc                                                                                                                 >  4:450013/1‑141 (MQ=255)

                            ccTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCt                                                                                                                <  2:752612/141‑1 (MQ=255)

                            ccTGTGTAAGCTTTATATTTAAATAATTTAAAATAGATTAAAGGATTTGAAGCACGAAAATCAGAAAATATAAATAAAAT‑AAAAGAACCAAATGCAAAAGCTAATAAAGTAAT‑AAAAAGAAGGAAGGTTATACATAATTCt                                                                                                                <  4:353689/141‑1 (MQ=255)

                             cTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTg                                                                                                               >  1:489811/1‑141 (MQ=255)

                                tgtAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTCGCACGCTTTTCACGATCTATCCATTAACA‑ATTAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATc                                                                                                            <  3:386470/141‑1 (MQ=255)

                                tgtAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATc                                                                                                            >  1:430003/1‑141 (MQ=255)

                                tgtAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATc                                                                                                            <  3:125681/141‑1 (MQ=255)

                                     gCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATTGCTAATAAAGTGAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTa                                                                                                       <  1:258062/141‑1 (MQ=255)

                                      cTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAg                                                                                                      >  2:464862/1‑141 (MQ=255)

                                         tATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTaa                                                                                                   >  1:384620/1‑141 (MQ=255)

                                           tttttAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAATAAAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAAt                                                                                                  <  4:453968/141‑1 (MQ=255)

                                           tttttAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAAtt                                                                                                 <  3:414712/141‑1 (MQ=255)

                                             tttAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTaa                                                                                               >  2:276509/1‑141 (MQ=255)

                                               tAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑ATAAAGAAGTGAGGTTACAACTAATTTTGATACTTTAGTAATTaaaa                                                                                             >  2:93385/1‑141 (MQ=255)

                                                aaaTAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAat                                                                                            >  2:784663/1‑141 (MQ=255)

                                                aaaTAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAat                                                                                            >  1:146024/1‑141 (MQ=255)

                                                    aatTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATAttt                                                                                        <  1:357440/141‑1 (MQ=255)

                                                     atTTAGGATCGATTAAAGGATTTGTAGCGCTCTCTTCAAGAACTATAAATAAACT‑AAAATATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTa                                                                                       <  3:202524/141‑1 (MQ=255)

                                                      tttAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATAAAAATATTTaa                                                                                      >  4:445990/1‑141 (MQ=255)

                                                       ttAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGCCCCTTGAGCAAGTAAAATATTTaaa                                                                                     <  2:1087204/141‑1 (MQ=255)

                                                       ttAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTaaa                                                                                     <  1:987687/141‑1 (MQ=255)

                                                            aTCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTgag                                                                                <  1:922957/141‑1 (MQ=255)

                                                               gATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTgaggag                                                                             >  1:851254/1‑141 (MQ=255)

                                                               gATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTgaggag                                                                             <  4:13332/141‑1 (MQ=255)

                                                                aTTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGc                                                                            >  2:1058614/1‑141 (MQ=255)

                                                                aTTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGc                                                                            <  1:178730/141‑1 (MQ=255)

                                                                     aGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACTAAAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATaa                                                                        <  1:259768/141‑1 (MQ=255)

                                                                       gATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGa                                                                     <  2:159974/141‑1 (MQ=255)

                                                                       gATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGa                                                                     <  2:1045051/141‑1 (MQ=255)

                                                                       gATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGa                                                                     <  4:212057/141‑1 (MQ=255)

                                                                        aTTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGAc                                                                    <  2:384753/141‑1 (MQ=255)

                                                                         tttGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACt                                                                   <  3:228979/141‑1 (MQ=255)

                                                                              aGCACGCATTTAAAGAACTAAAAATAAAAT‑AAAAGAAACAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATAAAAAAAAAAAAAAAATATTTAAACTGAGGAGCATAATGACTAAAAg                                                              <  4:227493/141‑1 (MQ=255)

                                                                               gCACGCTTTTCAAGAACTATAAAGAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATGCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGa                                                             <  1:49192/141‑1 (MQ=255)

                                                                                  cGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAAcc                                                          <  2:62617/141‑1 (MQ=255)

                                                                                   gCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCa                                                         <  2:820740/141‑1 (MQ=255)

                                                                                   gCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCa                                                         >  4:26856/1‑141 (MQ=255)

                                                                                   gCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAAGTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAACCTGAGGAGCATAATGACTAAAAGAACCa                                                         <  2:820599/141‑1 (MQ=255)

                                                                                    cTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAg                                                        <  1:206900/141‑1 (MQ=255)

                                                                                        tCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTTAGGAGCATAATGACTAAAAGAACCAGACCt                                                    >  1:1017476/1‑141 (MQ=255)

                                                                                        tCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCt                                                    >  1:627521/1‑141 (MQ=255)

                                                                                         cAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCtt                                                   >  2:51734/1‑141 (MQ=255)

                                                                                          aaGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCttt                                                  >  1:706461/1‑141 (MQ=255)

                                                                                          aaGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCttt                                                  <  1:996026/141‑1 (MQ=255)

                                                                                           aGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTa                                                 >  1:672172/1‑141 (MQ=255)

                                                                                              aCTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATg                                              >  2:524771/1‑141 (MQ=255)

                                                                                              aCTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATg                                              >  1:450000/1‑141 (MQ=255)

                                                                                               ttttaaataaaCT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGt                                             >  4:170317/4‑141 (MQ=255)

                                                                                               cTATAAAGAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGt                                             <  2:600263/141‑1 (MQ=255)

                                                                                                 ataaataaACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTTAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCa                                           >  4:322723/1‑141 (MQ=255)

                                                                                                   aaataaaCT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCaaa                                         >  4:162474/1‑141 (MQ=255)

                                                                                                    aataaaCA‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAat                                        <  2:861815/141‑1 (MQ=255)

                                                                                                     ataaaCT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAatt                                       <  4:333565/141‑1 (MQ=255)

                                                                                                        aaCT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAatttat                                    >  2:246247/1‑141 (MQ=255)

                                                                                                         aCT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAatttatt                                   <  2:491880/141‑1 (MQ=255)

                                                                                                          cT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAatttattt                                  >  2:533312/1‑141 (MQ=255)

                                                                                                           t‑AAAAGATCCAATTGCAATTGCTAATAAAGTGAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAAGTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAAtttattta                                 >  2:255155/1‑141 (MQ=255)

                                                                                                           t‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAG‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGATCCAGACCTGTAATGTCAAAtttattta                                 <  2:929235/141‑1 (MQ=255)

                                                                                                            aaaaaGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAAtttattta                                 <  2:797713/140‑1 (MQ=255)

                                                                                                              aaaGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTaga                               <  1:788129/141‑1 (MQ=255)

                                                                                                                  aTCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGaaa                           <  1:270338/141‑1 (MQ=255)

                                                                                                                     cAATTGCAAGAGCTATTAAATTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCg                        >  2:222334/1‑141 (MQ=255)

                                                                                                                      aaTTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCga                       <  1:37153/141‑1 (MQ=255)

                                                                                                                        ttGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatt                     <  1:388136/141‑1 (MQ=255)

                                                                                                                          gCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgattta                   >  2:163772/1‑141 (MQ=255)

                                                                                                                           cAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatttag                  >  3:299014/1‑141 (MQ=255)

                                                                                                                              ttgCTAATAAAGTGAT‑AAAAAGAAGTGAGGTTTCACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAAGTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatttagatt               <  1:571825/139‑1 (MQ=255)

                                                                                                                              ttgCTAATAAAGTGAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAAGTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatttagatt               <  2:573485/139‑1 (MQ=255)

                                                                                                                              tAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatttagatt               <  1:11819/141‑1 (MQ=255)

                                                                                                                              tAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCgatttagatt               <  1:11769/141‑1 (MQ=255)

                                                                                                                                     taaAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTc        >  1:688315/1‑141 (MQ=255)

                                                                                                                                     taaAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTc        <  2:326155/141‑1 (MQ=255)

                                                                                                                                      aaaGTGAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAAGTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCa       >  2:1059111/1‑141 (MQ=255)

                                                                                                                                        aGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCAgg     <  1:985818/141‑1 (MQ=255)

                                                                                                                                          taat‑aaAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCAGgtg   >  1:433785/1‑141 (MQ=255)

                                                                                                                                          taat‑aaAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACAAGACCTATAATGTCAAATTTCTTACAGGAAAAAGAGTTAGGTTTATTTTCAtgtg   >  3:198938/1‑141 (MQ=255)

                                                                                                                                           aat‑aaAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCAGgtgt  >  2:327671/1‑141 (MQ=255)

ATTTAACAAAAAGTTTGAAGCTGTTGCACCTGTGTAAGCTTTATTTTTAAATAATTTAAAATCGATTAAAGGATTTGTAGCACGCTTTTCAAGAACTATAAATAAACT‑AAAAGATCCAATTGCAATAGCTAATAAAGTAAT‑AAAAAGAAGTGAGGTTACACCTAATTCTGATCCTTTAGTAATTAAAATATTTAAACTGAGGAGCATAATGACTAAAAGAACCAGACCTTTAATGTCAAATTTATTTAGAGAAATCGATTTAGATTTAGTTTCAGGTGT  >  USA300TCH1516_ALE/1502973‑1503251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`