| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,963,502 | C→T | 6.7% | T453T (ACG→ACA) | hemY ← | Protoporphyrinogen oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,963,502 | 0 | C | T | 6.7% | 417.1 / 11.6 | 134 | T453T (ACG→ACA) | hemY | Protoporphyrinogen oxidase |
| Reads supporting (aligned to +/- strand): ref base C (57/68); new base T (3/6); total (60/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 135 total aligned reads displayed.
CACATTACTTCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACTTTTCGGCA > USA300TCH1516_ALE/1963362‑1963641 | cacaTTACTTCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGc > 1:965586/1‑141 (MQ=255) acaTTACTTCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCg > 1:1030471/1‑141 (MQ=255) tACTTCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAAt < 1:1033746/141‑1 (MQ=255) tACTTCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAAt < 2:95219/141‑1 (MQ=255) aCTTCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATa > 1:793478/1‑141 (MQ=255) cTTCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATAc < 2:1049067/141‑1 (MQ=255) cTTCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATAc < 2:774262/141‑1 (MQ=255) tCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACaa < 2:799811/141‑1 (MQ=255) tCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACaa < 2:855103/141‑1 (MQ=255) ccaccaTTATATAACTTAAAAATGACGATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAAt < 2:861242/141‑1 (MQ=255) ccaTTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGGGTGGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGAACCTTAACCATGCGAAAAACAACCAg > 3:115675/1‑141 (MQ=255) ccaTTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGCTTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAg > 1:1024986/1‑141 (MQ=255) tatataACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAg < 1:930744/141‑1 (MQ=255) atataACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGt > 1:89479/1‑141 (MQ=255) tataACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTc > 2:742718/1‑141 (MQ=255) tataACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGAGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTc < 2:130641/141‑1 (MQ=255) taACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCa > 2:818022/1‑141 (MQ=255) aCTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAAc > 1:60930/1‑141 (MQ=255) cTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAAcc < 2:291390/141‑1 (MQ=255) ttAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCg > 1:248576/1‑141 (MQ=255) aaaaTGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGcct > 1:773851/1‑139 (MQ=255) aaaaTGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCtt < 1:404770/141‑1 (MQ=255) aaaaTGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCtt < 2:748203/141‑1 (MQ=255) aaaaTGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCtt < 3:449985/141‑1 (MQ=255) aaTGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCa < 2:584825/141‑1 (MQ=255) aaTGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCa > 1:383050/1‑141 (MQ=255) aCTATATTTCATCAAACATTATCTCAAGGAGTAGCACCTACACAAACACAATCAAAAAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTACTCCACTCCGGTAGTGCAACCGCTTCAAAAg > 2:539786/1‑141 (MQ=255) aCTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAGCAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAg > 2:285504/1‑141 (MQ=255) aCTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAg > 2:370530/1‑141 (MQ=255) aCTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAg > 1:724810/1‑141 (MQ=255) cTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGa < 2:266386/141‑1 (MQ=255) tatTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCGTCAGCAGCAGCTTTACCTTGTGTAATACAGTCAGGTAGTCCAACCGCTTCAAAAGATGc < 2:1058923/141‑1 (MQ=255) catcaAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAg < 1:499259/141‑1 (MQ=255) tcaAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGtt > 1:831473/1‑141 (MQ=255) tcaAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGAACCAGtt < 1:898824/141‑1 (MQ=255) aaCATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTAct < 2:806641/141‑1 (MQ=255) aCATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTActc < 3:180421/141‑1 (MQ=255) aCATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTActc > 2:906384/1‑141 (MQ=255) cATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGTGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTActct > 1:396547/1‑141 (MQ=255) cATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTActct < 1:473257/141‑1 (MQ=255) cATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTActct < 1:765808/141‑1 (MQ=255) aTTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTa > 1:627387/1‑141 (MQ=255) tATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGTGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAg < 2:40228/141‑1 (MQ=255) aTCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGt < 1:663561/141‑1 (MQ=255) cTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCg < 1:294073/141‑1 (MQ=255) tAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGt > 2:683652/1‑141 (MQ=255) aGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGa < 4:376403/141‑1 (MQ=255) aGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGa > 1:744252/1‑141 (MQ=255) aGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGATTCGAAAGATGCACCAGTTACTCTAAGTCGTGGa < 2:976922/141‑1 (MQ=255) ggCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGat > 2:1060556/1‑141 (MQ=255) ggCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGat > 4:212391/1‑141 (MQ=255) cGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTCCGCCGTGGatat > 2:250654/1‑141 (MQ=255) tCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATAtgt > 2:62890/1‑141 (MQ=255) cGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATAtgtt > 1:906533/1‑141 (MQ=255) cGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATAtgtt > 3:422777/1‑141 (MQ=255) gCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATAtgttt > 1:125694/1‑141 (MQ=255) ccTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATAtgtttgtt > 1:840193/1‑141 (MQ=255) ccTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATAtgtttgtt > 1:513805/1‑141 (MQ=255) acaccaacaccaTCCGACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTGTACCTTGCGTAATACAATCGGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTaa < 1:242344/141‑1 (MQ=255) acaccaacaccaTCCAACAATTAACTTACAACGCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGACGCACCAGTTACTCTAAGTCGTGGATATgttggtttca > 2:227093/1‑139 (MQ=255) caccaaaaccaTCCAACAATTAACTTACAACTCAGCGATTACTTCTTCAGCAACCACTTTACCTAGCGTAATACAATCAGGTAGTCCAACAGCTTCAAACGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAat > 2:544604/1‑141 (MQ=255) accaacaccaTCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAATAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAata < 2:747022/141‑1 (MQ=255) caacaccaTCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATAtg < 3:106365/141‑1 (MQ=255) acaccaTCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTGATAtgtg < 1:860978/141‑1 (MQ=255) caccaTCCAACAATTAACTTACAACTCTGCGATTAGTTCTTCTGCAGCAACTTTACCTTGCGTAATAAAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGc > 1:934266/1‑141 (MQ=255) ccaTCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCtt > 1:608241/1‑141 (MQ=255) caTCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTg < 2:202190/141‑1 (MQ=255) aacaaTTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCt < 4:293304/141‑1 (MQ=255) aacaaTTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCt < 2:947954/141‑1 (MQ=255) caaTTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATCCAATCAGGTAGTCCAACCGCTTCAAACGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGt < 1:648553/141‑1 (MQ=255) aaCTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAAt > 3:403970/1‑141 (MQ=255) cAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATttgttg < 3:277291/141‑1 (MQ=255) cAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATttgttg < 2:510368/141‑1 (MQ=255) cAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATttgttg < 2:506305/141‑1 (MQ=255) cAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATttgttg < 2:418583/141‑1 (MQ=255) aCTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGaa < 1:329911/141‑1 (MQ=255) ctctGCGATTACTTCTTCAGCAGCAACTTTAGCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAat < 2:487364/141‑1 (MQ=255) tGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATg < 1:430959/141‑1 (MQ=255) tGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATg < 4:298004/141‑1 (MQ=255) gCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGa > 4:124709/1‑141 (MQ=255) gCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGa < 3:45033/141‑1 (MQ=255) gATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGAcc < 1:353738/141‑1 (MQ=255) tACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGAc < 1:226980/141‑1 (MQ=255) cttcttCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACAt > 3:98812/1‑141 (MQ=255) agcaACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTgg < 4:4415/141‑1 (MQ=255) gcaACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGc < 2:613990/141‑1 (MQ=255) aaCTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCAt < 3:285274/141‑1 (MQ=255) aCTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATa > 3:80612/1‑141 (MQ=255) tttACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACt > 3:382634/1‑141 (MQ=255) tttACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACt > 1:56899/1‑141 (MQ=255) ttACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGGTGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACtt < 1:762683/141‑1 (MQ=255) tACCTTGCGTAATACAATCAGGTAGTTCAACCGCTTCAAAATATGCACCAGTTACTCGAATGGTTTTTTATTTGTTTTTAATTTGGGTTTGATTCTGTCTAATTTGTTGTATATGATTGACATTGTACTTTGGCATAtttt > 3:224220/1‑141 (MQ=255) tACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTGTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACttt < 1:1053680/141‑1 (MQ=255) ccTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTACTATGTTCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATGCTTTTc > 2:60327/1‑141 (MQ=255) ccTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACttttt > 4:449574/1‑140 (MQ=255) ccTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACTTTTc < 1:746884/141‑1 (MQ=255) cTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACTTTTCg > 1:997740/1‑141 (MQ=255) ttGCTNAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACTTTTCgg < 3:141385/141‑1 (MQ=255) tGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACTTTTCGGc > 3:319927/1‑141 (MQ=255) gCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACTTTTCGGCa < 1:170652/141‑1 (MQ=255) | CACATTACTTCCACCATTATATAACTTAAAAATGACTATATTTCATCAAACATTATCTAAAGGCGTCGCACCTACACCAACACCATCCAACAATTAACTTACAACTCTGCGATTACTTCTTCAGCAGCAACTTTACCTTGCGTAATACAATCAGGTAGTCCAACCGCTTCAAAAGATGCACCAGTTACTCTAAGTCGTGGATATGTTTGTTTAATATGTGCTTGAATCTGTCTAATTTGTTGAATATGACCGACATGGTACTGTGGCATACTTTTCGGCA > USA300TCH1516_ALE/1963362‑1963641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |