BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,047,465	A→G	6.1%	S206P (TCG→CCG)	USA300HOU_RS10335 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,047,465	0	A	G	6.1%	375.5 / 11.7	131	S206P (TCG→CCG)	USA300HOU_RS10335	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (66/56); new base G (3/5); total (70/61)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01

Only 100 of 133 total aligned reads displayed.

ACTTGATTGCTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCTGGTGATTTTT  >  USA300TCH1516_ALE/2047325‑2047605

aCTTGATTGCTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGa                                                                                                                                              <  1:1042178/141‑1 (MQ=255)

  ttGATTGCTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATc                                                                                                                                            <  1:1083888/141‑1 (MQ=255)

   tGATTGCTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATcc                                                                                                                                           <  2:451110/141‑1 (MQ=255)

    gATTGCTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGGTCCa                                                                                                                                          >  2:302061/1‑141 (MQ=255)

     aTTGCTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCAt                                                                                                                                         <  1:448584/141‑1 (MQ=255)

      ttGCTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCAtc                                                                                                                                        >  1:1074345/1‑141 (MQ=255)

       tGCTTATATATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCAGTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTGGATCGAtct                                                                                                                                       <  4:434677/141‑1 (MQ=255)

       tGCTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCAtct                                                                                                                                       >  2:987617/1‑141 (MQ=255)

         cTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCAtcttt                                                                                                                                     >  3:75535/1‑141 (MQ=255)

         cTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCAtcttt                                                                                                                                     <  4:58506/141‑1 (MQ=255)

          ttATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCAtctttt                                                                                                                                    <  1:25811/141‑1 (MQ=255)

           tataAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCAtcttttt                                                                                                                                   >  1:619854/1‑141 (MQ=255)

                   gCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCttttttt                                                                                                                           >  2:870149/1‑140 (MQ=255)

                   gCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATAATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTta                                                                                                                           >  1:855422/1‑141 (MQ=255)

                    cAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTtat                                                                                                                          <  1:381991/141‑1 (MQ=255)

                     aaaaTGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTtata                                                                                                                         <  2:1002515/141‑1 (MQ=255)

                         tGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTACCCATTTTTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTATTTTACTAAAAATCGACCTATTTTTTTCTTTTTTATATtaa                                                                                                                     >  4:415382/1‑141 (MQ=255)

                          gAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGtaat                                                                                                                    >  3:321363/1‑141 (MQ=255)

                           aaTTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGtaata                                                                                                                   <  2:485442/141‑1 (MQ=255)

                             ttGGTATATCTTTAAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATaaa                                                                                                                 >  4:62756/1‑141 (MQ=255)

                              tGGTCTAGCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAac                                                                                                                <  2:856285/141‑1 (MQ=255)

                              tGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAac                                                                                                                <  1:776496/141‑1 (MQ=255)

                              tGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAac                                                                                                                <  2:27205/141‑1 (MQ=255)

                               ggTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAaca                                                                                                               <  1:621029/141‑1 (MQ=255)

                               ggTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAaca                                                                                                               <  1:39184/141‑1 (MQ=255)

                               ggTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAaca                                                                                                               >  2:263387/1‑141 (MQ=255)

                               ggTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAaca                                                                                                               <  2:493772/141‑1 (MQ=255)

                                      cTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCTTCTTTTTCTTTTTTATAGTTGTAAATACTGTTCa                                                                                                        >  1:772300/1‑141 (MQ=255)

                                      cTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCa                                                                                                        >  1:573816/1‑141 (MQ=255)

                                         ttAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAg                                                                                                     >  1:744672/1‑141 (MQ=255)

                                          tAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAATTCGATCCATCTTTTTCTTTTTTATAGTTATTAACACTATTCATAGc                                                                                                    >  2:192805/1‑141 (MQ=255)

                                            aaTCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTa                                                                                                  <  2:788679/141‑1 (MQ=255)

                                             atcatcATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTaa                                                                                                 >  2:696003/1‑141 (MQ=255)

                                              tcatcaTTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTaaa                                                                                                <  1:911968/141‑1 (MQ=255)

                                                atcatTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTTTTGTAATAAACACTATTCATAGCTAAAcc                                                                                              >  2:169644/1‑141 (MQ=255)

                                                 tcatTTTCACGTCGTGTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCa                                                                                             <  1:603792/141‑1 (MQ=255)

                                                 tcatTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTAGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTTATAGCTAAAcaa                                                                                             >  1:86916/1‑139 (MQ=255)

                                                  catTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCaa                                                                                            <  3:237935/141‑1 (MQ=255)

                                                     tttCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCg                                                                                         >  2:971127/1‑141 (MQ=255)

                                                     tttCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACGAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGATAAACCAATcc                                                                                         >  2:845118/1‑140 (MQ=255)

                                                         aCGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAACCACTATTCATAGCTAAACCAATCGTCAt                                                                                     >  1:705020/1‑141 (MQ=255)

                                                         aCGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCAt                                                                                     >  2:506964/1‑141 (MQ=255)

                                                         aCGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCAt                                                                                     <  2:619854/141‑1 (MQ=255)

                                                         aCGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACAAATNGTCAt                                                                                     >  1:2168/1‑141 (MQ=255)

                                                          cGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATa                                                                                    >  1:310724/1‑141 (MQ=255)

                                                            tAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGGTCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATAcc                                                                                  <  2:730567/141‑1 (MQ=255)

                                                                cTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTa                                                                              <  2:424916/141‑1 (MQ=255)

                                                                cTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTa                                                                              <  3:238417/141‑1 (MQ=255)

                                                                  tGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAat                                                                            >  1:208581/1‑141 (MQ=255)

                                                                  tGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAat                                                                            <  1:839881/141‑1 (MQ=255)

                                                                    ataataTCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAatat                                                                          >  4:375264/1‑141 (MQ=255)

                                                                     taataTCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATAtt                                                                         <  1:124616/141‑1 (MQ=255)

                                                                      aataTCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTc                                                                        <  2:923348/141‑1 (MQ=255)

                                                                       atatCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCt                                                                       <  2:316105/141‑1 (MQ=255)

                                                                        tatCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCtt                                                                      >  1:882283/1‑141 (MQ=255)

                                                                         atCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTa                                                                     >  3:303283/1‑141 (MQ=255)

                                                                         atCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTa                                                                     >  1:951671/1‑141 (MQ=255)

                                                                          tctcACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTAc                                                                    >  1:884242/1‑141 (MQ=255)

                                                                              aCTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCttt                                                                <  3:69828/141‑1 (MQ=255)

                                                                               cTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCtttt                                                               >  4:449638/1‑141 (MQ=255)

                                                                                  gCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAATACTATTCATAGCTTAACCAATCTTCATACCTTTAATCTTCTTAcccaatgtt                                                            >  2:314362/1‑134 (MQ=255)

                                                                                       ttGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTcc                                                       >  1:131776/1‑141 (MQ=255)

                                                                                       ttGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATACATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTcc                                                       >  1:20612/1‑141 (MQ=255)

                                                                                               cTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCAt                                               <  3:107331/141‑1 (MQ=255)

                                                                                               cTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCAt                                               <  3:107272/141‑1 (MQ=255)

                                                                                                 tGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATaa                                             <  2:938774/141‑1 (MQ=255)

                                                                                                  gCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATaaa                                            <  3:68420/141‑1 (MQ=255)

                                                                                                   cTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATaaaa                                           <  1:317247/141‑1 (MQ=255)

                                                                                                      tttAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATc                                        >  1:526207/1‑141 (MQ=255)

                                                                                                       ttAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCt                                       >  1:561949/1‑141 (MQ=255)

                                                                                                        tagcTTCGCTATCATCTAGATGTGTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTCATAAACACTTTTCATTTCTACACCCCTCGTCAAACCTTTAATATTTTGTCCTTTTGTTACTCCCCCTCCATAACATTCtt                                      <  4:258642/138‑1 (MQ=255)

                                                                                                        tAACTTCGCTATCATCTAGTTTTTTACTATAATTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCtt                                      >  2:249614/1‑141 (MQ=255)

                                                                                                          aCTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTAGTCATAGCTAAACCAGTCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGc                                    >  1:102778/1‑141 (MQ=255)

                                                                                                            ttCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGctc                                  <  1:660757/141‑1 (MQ=255)

                                                                                                            ttCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGctc                                  <  1:530028/141‑1 (MQ=255)

                                                                                                             tCGCTATCATCTAGTTTTTTACTAAAAGTCGGTCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGctct                                 <  2:744796/141‑1 (MQ=255)

                                                                                                             tCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGctct                                 >  1:715140/1‑141 (MQ=255)

                                                                                                             tCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGctct                                 <  3:229499/141‑1 (MQ=255)

                                                                                                             tCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGctct                                 <  1:696003/141‑1 (MQ=255)

                                                                                                             tCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACGATTCATAGCTAAGCCAAGCGTCATACCTGTATTATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGctct                                 >  3:340654/1‑141 (MQ=255)

                                                                                                               gCTAGCATCTAGTTTTTTACTAAAAGTCAACCCATCTTTTTCTTTTTTATAGTAATAAACCCTATTCATAGCTAAACCAGTCTTCATACCTTCAATATTCTCACCTTTTGCACCTCCATCACCATAAAAATCTTACTCTac                               >  4:222901/1‑140 (MQ=255)

                                                                                                                     atcTAGTTTTTTACTAAAAGTCGGTCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGtt                         >  3:184437/1‑141 (MQ=255)

                                                                                                                     atcTAGTTTTTTACTAAAAGTCGCTCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTATAATGtt                         >  1:39454/1‑141 (MQ=255)

                                                                                                                     atcTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTTCCTTTTGTGTCTCCACCCCCATAAAAATCTTGCTCTATAATGtt                         >  1:170489/1‑141 (MQ=255)

                                                                                                                     atcTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGtt                         >  2:737418/1‑141 (MQ=255)

                                                                                                                      tcTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTa                        >  1:835470/1‑141 (MQ=255)

                                                                                                                       cTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAg                       <  2:618569/141‑1 (MQ=255)

                                                                                                                          gTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATa                    <  1:1023474/141‑1 (MQ=255)

                                                                                                                          gTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATa                    >  1:662612/1‑141 (MQ=255)

                                                                                                                            tttttACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATaaa                  >  4:218357/1‑141 (MQ=255)

                                                                                                                                  cTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCt            >  1:189244/1‑141 (MQ=255)

                                                                                                                                    aaaaGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCTgg          <  1:759698/141‑1 (MQ=255)

                                                                                                                                     aaaGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCTGGt         <  2:954871/141‑1 (MQ=255)

                                                                                                                                      aaGTCGATCCATCTTTTTCTTTTTTTTTTTTTTAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATATAAATCTTGCTCTAAAATGTTATATAAATATGCTGGtt        >  2:118454/1‑140 (MQ=255)

                                                                                                                                      aaGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCTGGTg        <  2:40474/141‑1 (MQ=255)

                                                                                                                                      aaGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCTGGTg        >  1:685094/1‑141 (MQ=255)

                                                                                                                                          cGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCTGGTGAttt    >  1:549668/1‑141 (MQ=255)

                                                                                                                                           gATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCTGGTGAtttt   >  1:1000688/1‑141 (MQ=255)

                                                                                                                                           gATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCTGGTGAtttt   <  1:772569/141‑1 (MQ=255)

                                                                                                                                            aTCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCTGGTGAttttt  >  1:652307/1‑141 (MQ=255)

ACTTGATTGCTTATAAATTGCAAAATGAATTGGTATATCTTTTAAATCATCATTTTCACGTAACCTTGATAATATCTCACTAGCCATTTGTTTACCTTGCTTTTTAACTTCGCTATCATCTAGTTTTTTACTAAAAGTCGATCCATCTTTTTCTTTTTTATAGTAATAAACACTATTCATAGCTAAACCAATCGTCATACCTTTAATATTCTTACCTTTTGTATCTCCACCACCATAAAAATCTTGCTCTAAAATGTTAGATAAATAGGCTGGTGATTTTT  >  USA300TCH1516_ALE/2047325‑2047605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`