Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,257,055 C→T 5.3% intergenic (‑144/+56) dps ← / ← USA300HOU_RS11555 General stress protein 20U/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,257,0550CT 5.3% 403.2 / 8.4 134intergenic (‑144/+56)dps/USA300HOU_RS11555General stress protein 20U/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (70/54);  new base T (3/4);  total (75/59)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-01

Only 100 of 138 total aligned reads displayed.

AATACACTCCTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATTTGGTTGGTGCCATGTTGTTTTCCAC  >  USA300TCH1516_ALE/2256916‑2257195
                                                                                                                                           |                                                                                                                                            
 aTACACTCCTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCt                                                                                                                                            >  1:305833/1‑141 (MQ=255)
 aTACACTCCTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCt                                                                                                                                            >  1:136298/1‑141 (MQ=255)
 aTACACTCCTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCt                                                                                                                                            >  4:444981/1‑141 (MQ=255)
   acacTCCTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATGATTCTAATCTGTAAAGTGTTTTTACTCTCTTTTTTATAATTATTCTAATCTAAATGTAAGGTGTTCCGCTTTTTAAATGTCTaa                                                                                                                                          >  1:459801/1‑141 (MQ=255)
    cacTCCTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTaaa                                                                                                                                         >  1:291662/1‑141 (MQ=255)
    cacTCCTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTaaa                                                                                                                                         <  1:163645/141‑1 (MQ=255)
    cacTACTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTaaa                                                                                                                                         <  1:1042471/141‑1 (MQ=255)
     acTCCTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTTTAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAAt                                                                                                                                        >  2:981196/1‑141 (MQ=255)
         cTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAGTTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAAttttg                                                                                                                                    <  3:349323/141‑2 (MQ=255)
          ttAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTaa                                                                                                                                   >  2:7207/1‑141 (MQ=255)
                 tGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATtcatca                                                                                                                            >  3:52655/1‑141 (MQ=255)
                  gTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTcatcat                                                                                                                           <  1:635509/141‑1 (MQ=255)
                  gTCTACGTCTTGCTCGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGTTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTcatcat                                                                                                                           >  2:85910/1‑141 (MQ=255)
                    cTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCt                                                                                                                         <  1:68679/141‑1 (MQ=255)
                      aCGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTaa                                                                                                                       <  1:331923/141‑1 (MQ=255)
                       cGTCTTGCTTGTTTCTATTCATTATTATATTATAATAATTTTTATCTGTTCAGTGTTTTTGCTCTCTTTTTTTTAATTATGCTCAACTACCCGTGTAGCGTCCCGCCATTTATGTGCCTTAATATTAATTCctcatcctat                                                                                                                      <  2:584195/141‑5 (MQ=255)
                       cGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAat                                                                                                                      >  4:178465/1‑141 (MQ=255)
                         tcatgcatgTTTATATAAATTATCATATAATAATACTTTTAATATGTAAAGTGTTTTTGCTATCTTTTTTATAATTATTATAATCTAACCGTAAAGTGTTCCGCTATTTACATGACTAATTTTTAATTAATCATCTCatat                                                                                                                    >  2:267862/4‑141 (MQ=255)
                          cttgcttgTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAatata                                                                                                                   <  1:127337/141‑1 (MQ=255)
                           ttgcttgTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAatatat                                                                                                                  <  1:505867/141‑1 (MQ=255)
                             gcttgTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATcc                                                                                                                >  3:332158/1‑141 (MQ=255)
                             gcttgTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATcc                                                                                                                <  3:406946/141‑1 (MQ=255)
                              cttgTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCa                                                                                                               <  1:96889/141‑1 (MQ=255)
                               ttgTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCAt                                                                                                              <  3:275525/141‑1 (MQ=255)
                               ttgTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCAt                                                                                                              >  2:619224/1‑141 (MQ=255)
                               ttgTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCAt                                                                                                              <  2:841619/141‑1 (MQ=255)
                                 gTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTa                                                                                                            >  1:1050241/1‑141 (MQ=255)
                                   ttATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATc                                                                                                          >  2:589955/1‑141 (MQ=255)
                                   ttATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATc                                                                                                          >  1:734242/1‑141 (MQ=255)
                                    tataTTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCa                                                                                                         >  3:392008/1‑141 (MQ=255)
                                        ttaattaTTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTa                                                                                                     >  3:15882/1‑141 (MQ=255)
                                        ttaattaTTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTa                                                                                                     >  1:394137/1‑141 (MQ=255)
                                         taattaTTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAg                                                                                                    >  1:446140/1‑141 (MQ=255)
                                          aattaTTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTTTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGc                                                                                                   >  2:165202/1‑141 (MQ=255)
                                          aattaTTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGc                                                                                                   <  1:557265/141‑1 (MQ=255)
                                          aattaTTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGc                                                                                                   >  1:225867/1‑141 (MQ=255)
                                          aattaTTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGc                                                                                                   <  1:986818/141‑1 (MQ=255)
                                           attattATATTATAATAATTTTAATCTGTAAAGTGTTTTTTTTCTTTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCTATTATTAATTAGCa                                                                                                  >  2:780684/1‑141 (MQ=255)
                                             tattatattaTAATGATTCTAATCTGTAAAGTGTTTTTACTCTCTTTTTTATAATTATTCTAATCTAAATGTAAGGTGTTCCGCTTTTTAAATGTCTAAATTTTAATTCATCATCTAGTATATCCATTATCAATTAGCAAg                                                                                                <  2:389920/141‑1 (MQ=255)
                                             tattatattaTAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAg                                                                                                >  2:696338/1‑141 (MQ=255)
                                             tattatattaTAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAg                                                                                                <  2:119585/141‑1 (MQ=255)
                                              attatattatAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGt                                                                                               >  1:216415/1‑141 (MQ=255)
                                               ttatattataATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTa                                                                                              <  1:916862/141‑1 (MQ=255)
                                               ttatattataATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTa                                                                                              <  4:209595/141‑1 (MQ=255)
                                                tatattataATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTAt                                                                                             >  1:174164/1‑141 (MQ=255)
                                                 atattataATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATg                                                                                            >  2:820838/1‑141 (MQ=255)
                                                  tattataATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGt                                                                                           <  2:607169/141‑1 (MQ=255)
                                                       taataaTTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTatc                                                                                     <  1:1060892/141‑1 (MQ=255)
                                                           aatTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTatcaat                                                                                  >  4:257985/1‑141 (MQ=255)
                                                           aatTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTatcaat                                                                                  >  3:33075/1‑141 (MQ=255)
                                                           aatTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTatcaat                                                                                  >  1:663206/1‑141 (MQ=255)
                                                            atTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTACATAACCTAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTatcaatc                                                                                 >  1:565492/1‑141 (MQ=255)
                                                              tttAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAg                                                                               <  1:6303/141‑1 (MQ=255)
                                                              tttAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAg                                                                               <  2:646316/141‑1 (MQ=255)
                                                                 aaTCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTaa                                                                            >  1:159007/1‑141 (MQ=255)
                                                                 aaTCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTaa                                                                            >  1:578636/1‑141 (MQ=255)
                                                                  ttCTTTACAGTGTTTTTGCTCTCTTTTTTATAATTATTCTGATCTAACGGTAAAGTGTTTTGCTTTTTAATTGCCTAATTTTTATTTCTTCATCTAATATATCCATTATCAATTATCAATTTTGTTTTATCTATCAGTatt                                                                           >  2:481743/2‑139 (MQ=255)
                                                                   tCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGt                                                                          >  1:179697/1‑141 (MQ=255)
                                                                    cTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGtt                                                                         >  1:746549/1‑141 (MQ=255)
                                                                     tGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGttt                                                                        >  2:623132/1‑141 (MQ=255)
                                                                      gTAAAGTGTTTTTGCTCTCTTTTTTTTAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCATTTAGTAAGtttt                                                                       >  2:465711/1‑141 (MQ=255)
                                                                      gTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGtttt                                                                       >  2:496258/1‑141 (MQ=255)
                                                                        aaaGTGTTTTTACTCTCTTTTTTATAATTATTCTAATCTAAATGTAAGGTGTTCCGCTTTTTAAATGTCTAAATTTTAATTCATCATCTAGTATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTAc                                                                     <  2:459801/141‑1 (MQ=255)
                                                                           gtgtTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACttt                                                                  <  2:276561/141‑1 (MQ=255)
                                                                           gtgtTTTTGCTCTCTTTTTTATAATTATTCTAAGCTAACCGTAAAGTGTTCCGGTATTTAAATGCCTAAATTTTAATTCATCCTCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAGTCAGTAAGTTTTACttt                                                                  <  4:114313/141‑1 (MQ=255)
                                                                           gtgtTTTTGCTCTCTTTTTTATAATTATTATAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACttt                                                                  >  1:303182/1‑141 (MQ=255)
                                                                              tttttGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGc                                                               <  2:898146/141‑1 (MQ=255)
                                                                               ttttGCTCTCTTTTTTTTTTTTTTTTTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTTTTTATTTGTAATTTTTACTTTCGCa                                                              >  2:824989/1‑141 (MQ=255)
                                                                                tttGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCAt                                                             >  1:279132/1‑141 (MQ=255)
                                                                                tttGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCAt                                                             >  1:352787/1‑141 (MQ=255)
                                                                                tttGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCAt                                                             >  1:63358/1‑141 (MQ=255)
                                                                                  tttttttttttttATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTa                                                           >  4:286751/8‑141 (MQ=255)
                                                                                  tGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTa                                                           >  4:454031/1‑141 (MQ=255)
                                                                                    ctctctTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTatc                                                         <  2:214786/141‑1 (MQ=255)
                                                                                     tctctTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTatca                                                        >  1:400665/1‑141 (MQ=255)
                                                                                         ttttttATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTATTTCATCATTTAATATATCTTTTTTCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTa                                                    >  2:329758/1‑141 (MQ=255)
                                                                                          tttttATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTaa                                                   <  3:17735/141‑1 (MQ=255)
                                                                                             ttATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTaaaaa                                                >  3:52810/1‑141 (MQ=255)
                                                                                             ttATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTaaaaa                                                <  2:129163/141‑1 (MQ=255)
                                                                                              tataATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAg                                               >  1:577664/1‑141 (MQ=255)
                                                                                                taATTATTCTAATCTAAATGTAAGGTGTTCCGCTTTTTAAATGTCTAAATTTTAATTCATCATCTAGTATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGaa                                             >  3:367496/1‑141 (MQ=255)
                                                                                                    tattCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGAt                                         >  3:237295/1‑141 (MQ=255)
                                                                                                      ttCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGAttt                                       <  1:265164/141‑1 (MQ=255)
                                                                                                           atctaaCCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCAt                                  <  1:268757/141‑1 (MQ=255)
                                                                                                                 ccGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCAt                            >  3:308147/1‑141 (MQ=255)
                                                                                                                 ccGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCAt                            <  4:349521/141‑1 (MQ=255)
                                                                                                                 ccGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCAt                            <  1:665722/141‑1 (MQ=255)
                                                                                                                  cGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCAtt                           <  4:360989/141‑1 (MQ=255)
                                                                                                                    tAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATttg                         <  2:221384/141‑1 (MQ=255)
                                                                                                                     aaaGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATttgg                        >  2:71518/1‑141 (MQ=255)
                                                                                                                       aGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATttggtt                      >  1:601218/1‑141 (MQ=255)
                                                                                                                         tgctCCGCTATTTAAATGACTAAATTTTAATTCATCATCTCATATATCCATTCTCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATCATCATttggttgg                    <  4:133630/138‑1 (MQ=255)
                                                                                                                             ccGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATTTGGTTGGTGcc                >  3:311622/1‑141 (MQ=255)
                                                                                                                             ccGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATTTGGTTGGTGcc                >  3:22323/1‑141 (MQ=255)
                                                                                                                               gCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATTTGGTTGGTGCCAt              <  3:195863/141‑1 (MQ=255)
                                                                                                                                ctttttAAATGTCTAAATTTTAATTCATCATCTAGTATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATTTGGTTGGTGCCAtg             <  2:958620/138‑1 (MQ=255)
                                                                                                                                    ttAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATTTGGTTGGTGCCAtgttgt         >  1:602370/1‑141 (MQ=255)
                                                                                                                                    ttAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATTTGGTTGGTGCCAtgttgt         <  1:135148/141‑1 (MQ=255)
                                                                                                                                         tGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATTTGGTTGGTGCCATGTTGTTTTcc    >  1:591858/1‑141 (MQ=255)
                                                                                                                                           ccTAAATTTTAATTCATCATCTTATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATTTGGTTGGTGCCATGTTGTTTTCcac  <  2:305833/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AATACACTCCTTAAAATTGTCTACGTCTTGCTTGTTTATATTAATTATTATATTATAATAATTTTAATCTGTAAAGTGTTTTTGCTCTCTTTTTTATAATTATTCTAATCTAACCGTAAAGTGTTCCGCTATTTAAATGCCTAAATTTTAATTCATCATCTAATATATCCATTATCAATTAGCAAGTATGTTTTATCAATCAGTAAGTTTTACTTTCGCATTATCATCTAAAAAGAACGATTTTTCATAATCATTTGGTTGGTGCCATGTTGTTTTCCAC  >  USA300TCH1516_ALE/2256916‑2257195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: