BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,379,121	T→C	8.3%	G116G (GGA→GGG)	USA300HOU_RS12220 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,379,121	0	T	C	8.3%	413.2 / 24.3	158	G116G (GGA→GGG)	USA300HOU_RS12220	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (77/68); new base C (9/4); total (86/72)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-01

Only 100 of 160 total aligned reads displayed.

GGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATCATTTTCGCCACAA  >  USA300TCH1516_ALE/2378981‑2379260

ggCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAATGTACCTTCCTTCAAATAAATTTTCTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGt                                                                                                                                             >  1:1028646/1‑141 (MQ=255)

ggCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGt                                                                                                                                             >  2:438932/1‑141 (MQ=255)

 gCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTc                                                                                                                                            <  1:77333/141‑1 (MQ=255)

  cACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTcc                                                                                                                                           >  3:77290/1‑141 (MQ=255)

   aCTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTccc                                                                                                                                          <  1:181829/141‑1 (MQ=255)

   aCTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTccc                                                                                                                                          >  2:45864/1‑141 (MQ=255)

    cTGATTGTAATAATTTCTTACCTAAATATTTAAAATTGAATTGATAACTACCTTCACTAAATTAGCTACCTACAAAGTAAGTAATTTCAAACATCACAAAGGCACAACCTATATAAGCCATAACAAAATAAATAAGTCACg                                                                                                                                         >  2:84394/1‑141 (MQ=255)

    cTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTccca                                                                                                                                         <  2:241267/141‑2 (MQ=255)

    cTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTccca                                                                                                                                         <  2:241309/141‑2 (MQ=255)

      gATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGtt                                                                                                                                       <  2:726591/141‑1 (MQ=255)

       aTTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGttt                                                                                                                                      <  3:197286/141‑1 (MQ=255)

          gTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAg                                                                                                                                   >  1:997833/1‑141 (MQ=255)

          gTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAg                                                                                                                                   <  1:249898/141‑1 (MQ=255)

            aataatTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGc                                                                                                                                 >  1:429513/1‑141 (MQ=255)

             ataatTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGcc                                                                                                                                <  1:63965/141‑1 (MQ=255)

                atTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCata                                                                                                                             >  2:498402/1‑141 (MQ=255)

                 tttCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATACCAAAATAAATAAGCCCCGTTTTAGGCCatat                                                                                                                            <  2:251397/141‑1 (MQ=255)

                 tttCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCatat                                                                                                                            <  4:160110/141‑1 (MQ=255)

                   tCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCa                                                                                                                          >  3:321123/1‑141 (MQ=255)

                     ttACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACt                                                                                                                        <  2:558282/141‑1 (MQ=255)

                       aCCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTc                                                                                                                      >  1:285316/1‑141 (MQ=255)

                            aaTATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTa                                                                                                                 <  4:237282/141‑1 (MQ=255)

                                tttAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTgg                                                                                                             <  2:595232/141‑1 (MQ=255)

                                tttAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTgg                                                                                                             <  2:405081/141‑1 (MQ=255)

                                 ttAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGa                                                                                                            <  1:331726/141‑1 (MQ=255)

                                     aaTCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACCTCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATg                                                                                                        <  2:350895/141‑1 (MQ=255)

                                       tCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCa                                                                                                      <  4:313541/141‑1 (MQ=255)

                                        cGAATTGATCACTGCCATCACGAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAATTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCaa                                                                                                     <  4:380866/141‑1 (MQ=255)

                                        cGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCaa                                                                                                     <  1:235665/141‑1 (MQ=255)

                                        cGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCaa                                                                                                     <  1:155986/141‑1 (MQ=255)

                                         gAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTACTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCGCTTCAATTAGTGGATATGCaaa                                                                                                    >  3:379607/1‑141 (MQ=255)

                                         gAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCaca                                                                                                    >  1:689368/1‑139 (MQ=255)

                                          aaTTGATCACTACACTCACTAAAGTATCTACCTACAAAGTAAGTAATTCCAAACACCACAAAGGCACCACCTATATCAGCCATACCAAAATAAATAAGCCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAAc                                                                                                   <  2:859783/141‑1 (MQ=255)

                                           aTTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACACCACAAAGGCACCACCTATATCAGCCATACCAAAATAAATAAGCCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACa                                                                                                  <  2:32884/141‑1 (MQ=255)

                                             tGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAAt                                                                                                <  3:125866/141‑1 (MQ=255)

                                              gATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATc                                                                                               >  2:118202/1‑141 (MQ=255)

                                               aTCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATcc                                                                                              >  1:1043187/1‑141 (MQ=255)

                                                tCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCa                                                                                             <  2:991791/141‑1 (MQ=255)

                                                tCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCa                                                                                             <  3:443519/141‑1 (MQ=255)

                                                 cACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCaa                                                                                            <  2:754932/141‑1 (MQ=255)

                                                         tCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATa                                                                                    >  2:699896/1‑141 (MQ=255)

                                                         tCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATa                                                                                    >  4:337274/1‑141 (MQ=255)

                                                         tCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAAAAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATa                                                                                    >  2:148554/1‑141 (MQ=255)

                                                          cACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGata                                                                                                          >  2:213394/1‑118 (MQ=255)

                                                           aCTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATACCAAAATAAATAAGCCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGc                                                                                  >  3:412697/1‑141 (MQ=255)

                                                                aGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAg                                                                             >  2:865936/1‑141 (MQ=255)

                                                                aGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAg                                                                             >  2:962712/1‑141 (MQ=255)

                                                                aGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAg                                                                             >  2:508282/1‑141 (MQ=255)

                                                                   aGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGtca                                                                          <  1:970348/141‑1 (MQ=255)

                                                                    gCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGtcat                                                                         >  3:191957/1‑141 (MQ=255)

                                                                    gCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGtcat                                                                         <  4:118712/141‑1 (MQ=255)

                                                                    gCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGtcat                                                                         >  2:490591/1‑141 (MQ=255)

                                                                    gCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGtcaa                                                                         >  1:378122/1‑140 (MQ=255)

                                                                      tacctacAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGtcatca                                                                       >  2:697419/1‑141 (MQ=255)

                                                                              aagtaagtAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACt                                                               >  1:223390/1‑141 (MQ=255)

                                                                                gtaagtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGa                                                             <  1:426240/141‑1 (MQ=255)

                                                                                gtaagtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGa                                                             >  1:226011/1‑141 (MQ=255)

                                                                                 taagtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGAt                                                            >  3:448630/1‑141 (MQ=255)

                                                                                   agtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATcc                                                          >  1:847106/1‑141 (MQ=255)

                                                                                   agtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATcc                                                          >  1:884066/1‑141 (MQ=255)

                                                                                    gtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCa                                                         >  4:75566/1‑141 (MQ=255)

                                                                                    gtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCa                                                         >  1:608785/1‑141 (MQ=255)

                                                                                    gtaaTTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCa                                                         >  2:145331/1‑141 (MQ=255)

                                                                                      aagtcgaaaCATCCCGAAGGCACCACCTATATCAGCCATAACTAAATACATGAGTCCCGTTTTAGGCGTTATCACTTCACTAAGTTGGTATGCAAACAATCCAATACTCATAGCCCCCGCCATCATACCTTCTGATCCACg                                                       <  2:208760/135‑1 (MQ=255)

                                                                                        ttCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCa                                                     <  2:7756/141‑1 (MQ=255)

                                                                                        ttCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCa                                                     >  2:1050157/1‑141 (MQ=255)

                                                                                             aaCATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGa                                                >  2:912665/1‑141 (MQ=255)

                                                                                              aCATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGAt                                               >  2:349394/1‑141 (MQ=255)

                                                                                                aTCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTa                                             >  1:125177/1‑141 (MQ=255)

                                                                                                 tCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTAt                                            >  1:147475/1‑141 (MQ=255)

                                                                                                 tCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTAt                                            >  3:272514/1‑141 (MQ=255)

                                                                                                 tCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCAGATCACGTCAATTAGGGGAGATGCAAACAATCCAATATTCATATCACCAGTCATCATACTTACTGATCCACGCATGTGATTAt                                            >  1:758920/1‑141 (MQ=255)

                                                                                                  cgcgaaGGCACCGCCTATATCAGCCATAACAAAACAAATAAGTCCCCGTTTAGGCCATATCACTTCAAATAGTGGATATGCAAACAAGCCAATACGCATCGCACCAGGCATCATACCTTCTGATCCACGCATGTGATTATc                                           <  4:352546/137‑1 (MQ=255)

                                                                                                   acaAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCa                                          >  1:240114/1‑141 (MQ=255)

                                                                                                      aaGGCACCACCTATATCAGCCATAACAAAATCAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCCTAACAATCGGTGCATGCATGTGATTATCatat                                       <  4:457745/141‑1 (MQ=255)

                                                                                                      aaGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCatat                                       <  1:840973/141‑1 (MQ=255)

                                                                                                          caccacCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTc                                   <  1:530842/141‑1 (MQ=255)

                                                                                                           accaccTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCt                                  <  3:183723/141‑1 (MQ=255)

                                                                                                            ccaccTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTa                                 >  1:134736/1‑141 (MQ=255)

                                                                                                            ccaccTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTa                                 >  1:695942/1‑141 (MQ=255)

                                                                                                             caccTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTaa                                <  2:394631/141‑1 (MQ=255)

                                                                                                             caccTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTaa                                <  2:526005/141‑1 (MQ=255)

                                                                                                                cTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATGCCTACTGATCCACGCATGTGATTATCATATTTTCTaaaaa                             >  4:401924/1‑141 (MQ=255)

                                                                                                                      cAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGa                       >  1:813371/1‑141 (MQ=255)

                                                                                                                      cAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGa                       >  3:222281/1‑141 (MQ=255)

                                                                                                                       aGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGAc                      >  1:633803/1‑141 (MQ=255)

                                                                                                                        gCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTGCAATTAGTGGATATTCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACa                     <  1:99248/141‑1 (MQ=255)

                                                                                                                           aTAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCa                  >  3:447145/1‑141 (MQ=255)

                                                                                                                           aTAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCa                  <  2:1048807/141‑1 (MQ=255)

                                                                                                                               cAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATCa              >  1:379466/1‑141 (MQ=255)

                                                                                                                               cAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATCa              <  1:317255/141‑1 (MQ=255)

                                                                                                                                  aataaataAGCCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATCAttt           >  1:557521/1‑141 (MQ=255)

                                                                                                                                  aataaataAGCCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATCAttt           >  2:207211/1‑141 (MQ=255)

                                                                                                                                      aataaGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATAAAAAAAAAAAAACCAGACAGCAATCATTTTCGc       <  2:932059/141‑1 (MQ=255)

                                                                                                                                      aataaGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCAACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATCATTTTCGc       >  4:427424/1‑141 (MQ=255)

                                                                                                                                        taaGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTCAAAAACCAGACAGCAATCATTTTCGCca     >  3:403693/1‑141 (MQ=255)

                                                                                                                                        taaGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTATATATTCAGACAGCAATCATTTTCGCca     >  4:284879/1‑141 (MQ=255)

                                                                                                                                        taaGCCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATCATTTTCGCca     >  2:304176/1‑141 (MQ=255)

                                                                                                                                         aaGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATCATTTTCGCcac    <  1:722361/141‑1 (MQ=255)

                                                                                                                                           gTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATCATTTTCGCCACaa  >  1:867503/1‑141 (MQ=255)

GGCACTGATTGTAATAATTTCTTACCTAAATATTTAAAATCGAATTGATCACTACCTTCACTAAAGTAGCTACCTACAAAGTAAGTAATTCCAAACATCACAAAGGCACCACCTATATCAGCCATAACAAAATAAATAAGTCCCGTTTTAGGCCATATCACTTCAATTAGTGGATATGCAAACAATCCAATATTCATAGCACCAGTCATCATACCTACTGATCCACGCATGTGATTATCATATTTTCTAAAAAACCAGACAGCAATCATTTTCGCCACAA  >  USA300TCH1516_ALE/2378981‑2379260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`