Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 153,657 T→C 6.6% A420A (GCT→GCC rfbX → Putative O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE153,6570TC 6.6% 305.4 / 7.6 104A420A (GCT→GCCrfbXPutative O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base T (49/48);  new base C (4/3);  total (53/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 117 total aligned reads displayed.

ATATATTTTTATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCAATACGTATGGCAAA  >  USA300TCH1516_ALE/153517‑153796
                                                                                                                                            |                                                                                                                                           
atatatTTTTATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTgctgct                                                                                                                                             <  1:244177/141‑1 (MQ=255)
 tatatTTTTATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGctgctg                                                                                                                                            <  2:444108/141‑1 (MQ=255)
 tatatTTTTATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGctgctg                                                                                                                                            <  2:385219/141‑1 (MQ=255)
   tatTTTTATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAATATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGtt                                                                                                                                          >  2:862784/1‑141 (MQ=255)
    atTTTTATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTa                                                                                                                                         <  1:318369/141‑1 (MQ=255)
      ttttATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTatg                                                                                                                                       <  1:44707/141‑1 (MQ=255)
         tATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTatgatg                                                                                                                                    <  3:391606/141‑1 (MQ=255)
         tATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTatgatg                                                                                                                                    <  1:472864/141‑1 (MQ=255)
          aTGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGt                                                                                                                                   >  1:594353/1‑141 (MQ=255)
             gAATTTACGGTGCGGCTATTGCACGTTTAATTACAGAGTTTATCTTACTTATTTGGCGATTTGTTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCCGTTATGATGTTTa                                                                                                                                >  2:759909/1‑141 (MQ=255)
               aTTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTAtt                                                                                                                              >  2:790856/1‑141 (MQ=255)
               aTTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTAtt                                                                                                                              <  3:47021/141‑1 (MQ=255)
                tttACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTatatag                                                                                                                             >  1:817795/1‑139 (MQ=255)
                tttACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATtg                                                                                                                             <  4:133167/141‑1 (MQ=255)
                  tACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATtgtg                                                                                                                           >  3:74615/1‑141 (MQ=255)
                  tACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATtgtg                                                                                                                           <  1:682458/141‑1 (MQ=255)
                    cGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCt                                                                                                                         <  3:268953/141‑1 (MQ=255)
                    cGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCt                                                                                                                         <  1:316026/141‑1 (MQ=255)
                        gctgctATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTATGTACGATTCAATGTGTCATTGCTGCCGTTATGATGTTTATTGTGCTTGgt                                                                                                                     <  2:605777/141‑1 (MQ=255)
                        gctgctATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGgt                                                                                                                     <  2:664000/141‑1 (MQ=255)
                          tgctATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGgtgt                                                                                                                   <  3:225235/141‑1 (MQ=255)
                            ctATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTgg                                                                                                                 <  4:60703/141‑1 (MQ=255)
                            ctATTGCACGTTTAATTACAGAGTTTATCTTACTTATTTGGCGATTTGTTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCCGTTATGATGTTTATTGTGCTTGGTGTgg                                                                                                                 <  1:506873/141‑1 (MQ=255)
                             tATTGCGCGTTTAAGTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGt                                                                                                                <  2:77708/141‑1 (MQ=255)
                              aTTGCACGTTTAATTACAGAGTTTATCTTACTTATTTGGCGATTTGTTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCCGTTATGATGTTTATTGTGCTTGGTGTGGTc                                                                                                               >  2:776094/1‑141 (MQ=255)
                               ttGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCa                                                                                                              <  2:180452/141‑1 (MQ=255)
                                tGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCaa                                                                                                             <  2:192992/141‑1 (MQ=255)
                                  cgcgTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTATAAGTACGATTCAATGTGTCATTGATGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATc                                                                                                           >  2:999479/1‑141 (MQ=255)
                                    cgTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCat                                                                                                         <  3:435050/141‑1 (MQ=255)
                                    cgTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCat                                                                                                         <  1:666985/141‑1 (MQ=255)
                                     gTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCatt                                                                                                        <  2:369240/141‑1 (MQ=255)
                                          aTTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTg                                                                                                   <  2:157741/141‑1 (MQ=255)
                                           ttACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGc                                                                                                  >  3:194333/1‑141 (MQ=255)
                                            tACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGcc                                                                                                 >  2:488365/1‑141 (MQ=255)
                                            tACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGcc                                                                                                 >  2:393024/1‑141 (MQ=255)
                                              cAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTTTTGTGGTCAATCATTATTTGcccc                                                                                               >  4:317318/1‑141 (MQ=255)
                                                gagTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCt                                                                                             >  1:768772/1‑141 (MQ=255)
                                                  gTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTAc                                                                                           >  2:1080649/1‑141 (MQ=255)
                                                  gTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTAc                                                                                           >  2:76263/1‑141 (MQ=255)
                                                        cTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGta                                                                                     >  4:297113/1‑141 (MQ=255)
                                                          tGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATAATAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTAATTGCCCCCTACAATGtacg                                                                                   >  2:227858/1‑141 (MQ=255)
                                                              taTTTGGCGATTTGTTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCCGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGtacgctac                                                                               <  1:810553/140‑1 (MQ=255)
                                                              cATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGtacgctac                                                                               >  1:485179/1‑141 (MQ=255)
                                                                tttGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGtacgctacgc                                                                             >  3:188125/1‑141 (MQ=255)
                                                                 ttGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACACTGTacgctacgct                                                                            >  4:318531/1‑141 (MQ=255)
                                                                 ttGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTacgctacgct                                                                            >  2:48995/1‑141 (MQ=255)
                                                                   ggCGATTTATTGATATTAATAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACgctgc                                                                          <  2:727756/141‑1 (MQ=255)
                                                                    gCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACgctgct                                                                         >  4:75279/1‑141 (MQ=255)
                                                                    gCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACgctgct                                                                         >  3:232022/1‑141 (MQ=255)
                                                                      gATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGTTGCGGTAAATCATTATTTGCGTCCTACAATTTACGCTATGCTGCTAt                                                                       >  2:99904/1‑141 (MQ=255)
                                                                        tttATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTa                                                                     >  4:194600/1‑141 (MQ=255)
                                                                        tttATTGATATTACTAAAATCAATGCGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTa                                                                     >  1:395527/1‑141 (MQ=255)
                                                                            ttGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTg                                                                 <  2:392362/141‑1 (MQ=255)
                                                                            ttGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTg                                                                 <  2:1040262/141‑1 (MQ=255)
                                                                              gATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCg                                                               <  1:846487/141‑1 (MQ=255)
                                                                               atatTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGa                                                              >  2:494170/1‑141 (MQ=255)
                                                                                tatTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGAt                                                             <  1:453910/141‑1 (MQ=255)
                                                                                     cTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGta                                                        >  1:186390/1‑141 (MQ=255)
                                                                                        aaaTCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTAtag                                                     >  1:6115/1‑141 (MQ=255)
                                                                                        aaaTCAATGTGAAGCTGAATACAGTACGTACGCTTGAAGGTTTCATGGCTTCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTAtag                                                     <  1:585197/141‑1 (MQ=255)
                                                                                         aaTCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTCTGATGTTTATCGTGATTGGTCCGGTCAATCATTATTTGCCCCGTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTAtagt                                                    <  3:317318/141‑1 (MQ=255)
                                                                                           tCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTAtagtag                                                  >  1:604850/1‑141 (MQ=255)
                                                                                           tCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCCGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTAtagtag                                                  >  1:763668/1‑141 (MQ=255)
                                                                                            cAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATagtagt                                                 <  1:48769/141‑1 (MQ=255)
                                                                                            cAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATagtagt                                                 >  1:1012337/1‑141 (MQ=255)
                                                                                             aaTGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGtt                                                <  4:444836/141‑1 (MQ=255)
                                                                                              aTGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGttt                                               >  4:124097/1‑141 (MQ=255)
                                                                                              aTGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCCGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGttt                                               >  3:160645/1‑141 (MQ=255)
                                                                                               tgtgAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTa                                              >  4:432755/1‑141 (MQ=255)
                                                                                                gtgAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAGTTGCGATTGGTATAGTAGTTTAt                                             >  4:48828/1‑141 (MQ=255)
                                                                                                  gAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCt                                           <  1:544928/141‑1 (MQ=255)
                                                                                                    aGTTGATTCTTGTAAGTACGATTCAATGTGTCATTGATGCTGTTATGATGTGTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTCCGCTACGGTGCTATTAATTGCGATTGGTATAGTAGTTTATCttt                                         <  4:24017/141‑1 (MQ=255)
                                                                                                    aGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCttt                                         <  1:355816/141‑1 (MQ=255)
                                                                                                     gTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCtttt                                        <  1:695996/141‑1 (MQ=255)
                                                                                                       tGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGTTCGAGTAGTTTATCTTttat                                      >  3:115523/1‑141 (MQ=255)
                                                                                                          atatTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTaa                                   <  4:316432/141‑1 (MQ=255)
                                                                                                          atatTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTaa                                   >  2:991676/1‑141 (MQ=255)
                                                                                                                 aaGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGAc                            >  2:40988/1‑141 (MQ=255)
                                                                                                                   gTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTa                          <  2:594353/141‑1 (MQ=255)
                                                                                                                   gTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTa                          <  1:444035/141‑1 (MQ=255)
                                                                                                                    tACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTAt                         <  2:65734/141‑1 (MQ=255)
                                                                                                                     ccGAGTCAATGTGTCATTGCTGCTGTTATGATGTCTATTGTGCTTGGTGTGGTCAATCATTATTTGCACCCCACAATGTACGCTACGCTCCTATTAATTGCGATTAGAATACTAGTGTATCTTTTATTAATGATGGCTATg                        <  2:132891/140‑1 (MQ=255)
                                                                                                                     aCGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATg                        >  3:300084/1‑141 (MQ=255)
                                                                                                                      cGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGa                       >  2:563596/1‑141 (MQ=255)
                                                                                                                       gATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGaa                      >  1:441867/1‑141 (MQ=255)
                                                                                                                          tCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGaaaaa                   <  2:638574/141‑1 (MQ=255)
                                                                                                                             aTGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCa                >  2:686121/1‑141 (MQ=255)
                                                                                                                             aTGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCa                >  2:133146/1‑141 (MQ=255)
                                                                                                                              tgtgTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCaa               <  1:499300/141‑1 (MQ=255)
                                                                                                                              tgtgTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCaa               >  1:818002/1‑141 (MQ=255)
                                                                                                                                tgtCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCAATa             >  1:742963/1‑141 (MQ=255)
                                                                                                                                     ttGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCAATACGTAt        >  3:132556/1‑141 (MQ=255)
                                                                                                                                      tgctgcTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCAATACGTATg       >  3:246036/1‑141 (MQ=255)
                                                                                                                                         tgctgTTATGATGTTTCTTCGGGTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCAATACGTATGGCa    <  1:555866/141‑1 (MQ=255)
                                                                                                                                         tgctgTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCAATACGTATGGCa    <  4:109511/141‑1 (MQ=255)
                                                                                                                                         tgatgTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAAAAAAAAAAAAAAAAAATCAATACGTATGGCa    <  2:946581/138‑1 (MQ=255)
                                                                                                                                          gctgTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCAATACGTATGGCaa   >  1:422649/1‑141 (MQ=255)
                                                                                                                                          gctgTTATGATGTTTATTGTGCTTGGTGTGGTCAATCAGTATTTGCCCCCTACAATTTACGCTACGCGGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGAGGACTATGAAAAGTCAATACGTATGGCaa   >  2:647511/1‑141 (MQ=255)
                                                                                                                                           ctgTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTCCGCTACGCTGCTATTAATTGCGATAGGTAAAGTAGTTTAAACATTGTTAATGATGACTATGAAAAATCAATACGTATGGCaaa  >  1:158139/1‑141 (MQ=255)
                                                                                                                                           ctgTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCAATACGTATGGCaaa  >  3:198412/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
ATATATTTTTATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGATATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCTGTTATGATGTTTATTGTGCTTGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTTTATTAATGATGACTATGAAAAATCAATACGTATGGCAAA  >  USA300TCH1516_ALE/153517‑153796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: