BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,716,934	T→C	6.8%	T96A (ACA→GCA)	USA300HOU_RS13970 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,716,934	0	T	C	6.8%	387.0 / 13.7	132	T96A (ACA→GCA)	USA300HOU_RS13970	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (55/68); new base C (2/7); total (57/75)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 139 total aligned reads displayed.

CTTCATCTCTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGTTTTCTATTAAAA  >  USA300TCH1516_ALE/2716795‑2717074

cTTCATCTCTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACtgtg                                                                                                                                             <  3:255865/141‑1 (MQ=255)

    agctctGTATGGGCTTCTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGGCTGTGCCAc                                                                                                                                         <  2:675801/139‑1 (MQ=255)

      ctctGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTCGCAGACCAAACCATCAAACAGGTGTCCATCATGATCATTTAACTTTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCAccc                                                                                                                                       <  3:433011/141‑1 (MQ=255)

      ctctGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTTGTTCTTGAACGACTGTGCCAccc                                                                                                                                       >  4:245132/1‑141 (MQ=255)

      ctctGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCAccc                                                                                                                                       <  1:169709/141‑1 (MQ=255)

       tctGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATACAACCCTTGGCTTACTGTTGGTTCTGGAACGACTGTGCCACCCg                                                                                                                                      <  2:584353/141‑1 (MQ=255)

       tctGTATGGGCTCTTTATATATTCTTTTGAGAATTTCATTTAAGTATAACAAAAAATTAAAATGGTGTCCAGCGAGATCTTTGAACATTGCTGCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCg                                                                                                                                      <  2:979089/141‑1 (MQ=255)

        ctGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACTAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGCGCCACCCGc                                                                                                                                     <  1:127750/141‑1 (MQ=255)

         tGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGct                                                                                                                                    <  1:465310/141‑1 (MQ=255)

          gtatgggctttTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGctt                                                                                                                                   >  1:976723‑M2/12‑141 (MQ=255)

              gggctttTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCttt                                                                                                                               <  3:281017‑M2/134‑1 (MQ=255)

              gggctttTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCttt                                                                                                                               <  3:280986‑M2/134‑1 (MQ=255)

               ggctttTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTc                                                                                                                              <  1:761625‑M2/135‑1 (MQ=255)

                 ctttTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCAc                                                                                                                            >  1:1015772‑M2/5‑141 (MQ=255)

                 ctttTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCAc                                                                                                                            <  1:864792‑M2/137‑1 (MQ=255)

                   ttTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACtt                                                                                                                          >  2:439298‑M2/3‑141 (MQ=255)

                    tTTTTTTTTTTTTTTGAGAATTTAATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACCTTGCTCCATAAAACCCTTGGCTTATTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACttt                                                                                                                         >  4:146106‑M2/2‑141 (MQ=255)

                     ttatttatTCTTTTGAGAATTTCATTTTAGCAGCCCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTa                                                                                                                        <  3:75438/141‑1 (MQ=255)

                       atttattCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTAtt                                                                                                                      <  1:475447/141‑1 (MQ=255)

                       atttattCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTAtt                                                                                                                      <  2:1002829/141‑1 (MQ=255)

                       aattattCTTTTGAGAATTTCATATTACGAGACCAGAATATTAAAATGGAGTCCATCAAGAGCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTATAACGACTGTGCCACCCGCTTCGTTCACTTTCtt                                                                                                                      >  2:107248/3‑141 (MQ=255)

                        tttattCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTc                                                                                                                     <  1:546857/141‑1 (MQ=255)

                          tattCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCAc                                                                                                                   <  1:123879/141‑1 (MQ=255)

                           attCTTTTGAGAATTTCATTTTAGCAGACCAAATAATTAAAATGGTGTCCATCATGATCTTTGAACATTGCTCCATAAAACCCTATGCTGACTGTTGGATCTTTAATGACTGTGCCACCCTCTTCTTTAACTTTATTCAcc                                                                                                                  >  2:905695/1‑141 (MQ=255)

                           attCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCAcc                                                                                                                  >  2:421506/1‑141 (MQ=255)

                            ttCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTTTTCACCa                                                                                                                 >  2:729182/1‑141 (MQ=255)

                            ttCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCa                                                                                                                 >  2:78562/1‑141 (MQ=255)

                              cTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAAc                                                                                                               <  3:14648/141‑1 (MQ=255)

                                 ttGAGAATTTCATTTTAGCAGACTAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCACCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGCGCCACCCGCTTCTTTCACTTTATTCACCAACTgg                                                                                                            <  1:832080/141‑1 (MQ=255)

                                  tGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCCTCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGt                                                                                                           <  1:582385/141‑1 (MQ=255)

                                  tGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGt                                                                                                           <  1:1071537/141‑1 (MQ=255)

                                   gagaATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTc                                                                                                          <  1:477828/141‑1 (MQ=255)

                                     gaATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCaa                                                                                                        <  2:993883/141‑1 (MQ=255)

                                     gaATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCaa                                                                                                        >  1:32231/1‑141 (MQ=255)

                                     gaATTTCATTTTAGCAGACAAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCaa                                                                                                        <  4:280970/141‑1 (MQ=255)

                                        tttCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAActt                                                                                                     <  1:681290/141‑1 (MQ=255)

                                        tttCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAActt                                                                                                     >  2:151822/1‑141 (MQ=255)

                                         ttCATTTTAGCAGACCAAACAAGTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAActtc                                                                                                    <  3:94505/141‑1 (MQ=255)

                                         ttCATTTTAGCAGACCAAAAGATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAActtc                                                                                                    <  4:279978/141‑1 (MQ=255)

                                         ttCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAActtc                                                                                                    <  2:531572/141‑1 (MQ=255)

                                         ttCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAActtc                                                                                                    <  3:130073/141‑1 (MQ=255)

                                             ttttAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCa                                                                                                <  2:22461/141‑1 (MQ=255)

                                             ttttAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCa                                                                                                >  1:105164/1‑141 (MQ=255)

                                             ttttAGCAGACCAAAAAATTAAAATGGTGGCCATCAAGCTCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCa                                                                                                >  4:337620/1‑141 (MQ=255)

                                              tttAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCAt                                                                                               >  3:51007/1‑141 (MQ=255)

                                               ttAGCAGAACAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCAtt                                                                                              <  2:354589/141‑1 (MQ=255)

                                                 aGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTtg                                                                                            <  2:225638/141‑1 (MQ=255)

                                                  gCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTtgt                                                                                           >  2:137571/1‑141 (MQ=255)

                                                    aGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTgtgt                                                                                         <  1:643146/141‑1 (MQ=255)

                                                    aGACAAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTgtgt                                                                                         <  2:752472/141‑1 (MQ=255)

                                                      aCCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTc                                                                                       >  1:517672/1‑141 (MQ=255)

                                                      aCCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTc                                                                                       >  2:931052/1‑141 (MQ=255)

                                                      aCCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTc                                                                                       >  1:517760/1‑141 (MQ=255)

                                                       ccAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACTACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTca                                                                                      >  1:818234/1‑141 (MQ=255)

                                                              aTTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTag                                                                               <  2:351320/141‑1 (MQ=255)

                                                                tAAAATGGTGTCCATCAAGATCTTTGAACCTTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTagag                                                                             <  2:377247/141‑1 (MQ=255)

                                                                tAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTagag                                                                             <  1:583623/141‑1 (MQ=255)

                                                                  aaaTGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGaa                                                                           >  4:431060/1‑141 (MQ=255)

                                                                   aaTGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAGCGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGaaa                                                                          >  1:57673/1‑141 (MQ=255)

                                                                           ccATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACt                                                                  >  2:717006/1‑141 (MQ=255)

                                                                            cATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTTTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACtt                                                                 <  1:293642/141‑1 (MQ=255)

                                                                             aTCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTc                                                                <  3:97843/141‑1 (MQ=255)

                                                                               cAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCAt                                                              <  2:875722/141‑1 (MQ=255)

                                                                                   aTCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTgg                                                          >  1:1045654/1‑141 (MQ=255)

                                                                                   aTCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTgg                                                          <  2:366486/141‑1 (MQ=255)

                                                                                    tCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGt                                                         <  2:558726/141‑1 (MQ=255)

                                                                                            cATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTAtt                                                 <  4:7995/141‑1 (MQ=255)

                                                                                              ttGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTaa                                               <  2:198260/141‑1 (MQ=255)

                                                                                               tGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAg                                              >  1:477712/1‑141 (MQ=255)

                                                                                                gCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAgg                                             <  3:402708/141‑1 (MQ=255)

                                                                                                 cTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGc                                            <  3:215343/141‑1 (MQ=255)

                                                                                                     aTAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTg                                        >  1:897197/1‑141 (MQ=255)

                                                                                                     aTAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTg                                        >  1:897117/1‑141 (MQ=255)

                                                                                                          aCCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGAc                                   >  3:134688/1‑141 (MQ=255)

                                                                                                             cTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGCCTTa                                >  1:176871/1‑141 (MQ=255)

                                                                                                             cTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGCCTTa                                >  1:176788/1‑141 (MQ=255)

                                                                                                             cTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTa                                >  1:720262/1‑141 (MQ=255)

                                                                                                                  cTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACAttt                           >  4:407989/1‑141 (MQ=255)

                                                                                                                  cTTACTGTGGGTTCTTGAACGACTGTGCCACCCGCTTCGTTCACGTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTGCAGTATTATGGCGTGGCTGACTTACAttt                           >  4:456285/1‑141 (MQ=255)

                                                                                                                    tACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTg                         <  1:988871/141‑1 (MQ=255)

                                                                                                                          tGGTTCTTGAACGACTGCGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATg                   <  1:404864/141‑1 (MQ=255)

                                                                                                                          tGGTTCTTGAACGACTGCGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATg                   <  1:11061/141‑1 (MQ=255)

                                                                                                                          tGGTTCTTGAACGACTGCGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATg                   <  3:295485/141‑1 (MQ=255)

                                                                                                                           ggTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGc                  <  2:418280/141‑1 (MQ=255)

                                                                                                                           ggTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGc                  <  3:334609/141‑1 (MQ=255)

                                                                                                                           ggTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGc                  <  2:636101/141‑1 (MQ=255)

                                                                                                                             ttCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCtt                <  3:91140/141‑1 (MQ=255)

                                                                                                                             ttCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCtt                <  2:5576/141‑1 (MQ=255)

                                                                                                                             ttCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTGACATTTTGAAAATGCtt                <  3:91145/141‑1 (MQ=255)

                                                                                                                               cTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGt              >  2:34645/1‑141 (MQ=255)

                                                                                                                               cTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTACATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGt              <  2:105164/141‑1 (MQ=255)

                                                                                                                                 tGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGttt            <  2:417696/141‑1 (MQ=255)

                                                                                                                                 tGAACGACTGCGCCACCCGCTTCTTTCACTTTATTCACCGACTTTTCAACTTCTGCATTGTTTTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGttt            <  1:644345/141‑1 (MQ=255)

                                                                                                                                     cGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGTTTTCTa        >  1:1060264/1‑141 (MQ=255)

                                                                                                                                        cTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGTTTTCTATTa     <  4:299064/141‑1 (MQ=255)

                                                                                                                                          gtgCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGTTTTCTATTaaa   <  2:876581/141‑1 (MQ=255)

                                                                                                                                          gtgCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTCTTTTCTATTaaa   >  2:733192/1‑141 (MQ=255)

                                                                                                                                          ctgCCACCCGGTTCTTTCACTTTACTCACACACTGGTCATCTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGTTTTCTATTaaa   <  4:87515/140‑1 (MQ=255)

                                                                                                                                           tgCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCACCTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGTTTTCTATTaaca  >  2:904450/1‑139 (MQ=255)

                                                                                                                                           tgCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGTTTTCTATTaaaa  >  2:558702/1‑141 (MQ=255)

CTTCATCTCTGTATGGGCTTTTTATTTATTCTTTTGAGAATTTCATTTTAGCAGACCAAAAAATTAAAATGGTGTCCATCAAGATCTTTGAACATTGCTCCATAAAACCCTTGGCTTACTGTTGGTTCTTGAACGACTGTGCCACCCGCTTCTTTCACTTTATTCACCAACTGGTCAACTTCTTCATTTGTGTTCACACCTAGAGAAATCATCACTTCATTTGGTTCAGTATTAAGGCTTTGCTGACTTACATTTTGAAAATGCTTGTTTTCTATTAAAA  >  USA300TCH1516_ALE/2716795‑2717074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`