BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	267,167	G→A	6.0%	T290T (ACG→ACA)	USA300HOU_RS01175 →	Staphylocoagulase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	267,167	0	G	A	6.0%	347.9 / 5.1	122	T290T (ACG→ACA)	USA300HOU_RS01175	Staphylocoagulase
Reads supporting (aligned to +/- strand): ref base G (58/56); new base A (2/6); total (60/62)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 125 total aligned reads displayed.

TTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTAAAGTTGATAACCAACA  >  USA300TCH1516_ALE/267027‑267307

ttAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACg                                                                                                                                              >  3:167463/1‑141 (MQ=255)

       cAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAg                                                                                                                                       <  1:1001932/141‑1 (MQ=255)

        aaTTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGc                                                                                                                                      >  3:258496/1‑141 (MQ=255)

         attattGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCa                                                                                                                                     <  1:754379/141‑1 (MQ=255)

         attattGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCa                                                                                                                                     >  1:560893/1‑141 (MQ=255)

         attattGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCa                                                                                                                                     <  2:34039/141‑1 (MQ=255)

         attattGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCa                                                                                                                                     >  3:450550/1‑141 (MQ=255)

         attattGATGATTTCTTAATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCATACAACAAATAACTATAAAACAAATAGTGATAATAAACCTAAATTAGATAAATTAGTTGAAGAAACGAAAAAAGCa                                                                                                                                     >  2:413009/1‑141 (MQ=255)

              tgatgaTTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTaa                                                                                                                                >  3:305325/1‑141 (MQ=255)

              tgatgaTTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTaa                                                                                                                                >  2:678302/1‑141 (MQ=255)

              tgatcaTTTCTTAAAGGAAAAAAAACAAAAAAGACCGAAATCTACAAAGAAATATAATCAAACAACACATAACTATAAAACAAAGAGAGATAATAAACCTAATTTTGATAATGTAAAAAAAAAAAAAAAAAAAGCAGTTaa                                                                                                                                <  4:54333/141‑1 (MQ=255)

               gatgatTTCTTTATAGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTaaa                                                                                                                               <  2:213519/141‑1 (MQ=255)

                   atTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAaagaag                                                                                                                           <  1:225870/141‑1 (MQ=255)

                        tttATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAaagaatcatct                                                                                                                      <  3:241200/141‑7 (MQ=255)

                        tttATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAgat                                                                                                                      <  1:226744/141‑1 (MQ=255)

                         ttATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAgatg                                                                                                                     >  2:444833/1‑141 (MQ=255)

                          aaTGGAAACTAAACAAAAGAGACCGAAATATATAACGAAATATAAAACGACAACACATAACTATAAAACAAATAGTGATAATAAACGTAATTTTGATAAATTAGTAAAAAAAAAAAAAAAAGCAGTTAAAGAAGCAgatga                                                                                                                    <  2:944759/140‑1 (MQ=255)

                           aTGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAgatgat                                                                                                                   >  2:794908/1‑141 (MQ=255)

                             ggAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTc                                                                                                                 <  1:708669/141‑1 (MQ=255)

                              gAAACTAAACAAAATAGACCGAAATCTATAAAGAAATATAAACCTAAAACACATAAAAAAAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCt                                                                                                                <  2:228168/141‑1 (MQ=255)

                               aaaCTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCtt                                                                                                               <  1:698688/141‑1 (MQ=255)

                                aaCTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTg                                                                                                              >  1:913259/1‑141 (MQ=255)

                                 aCTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTgg                                                                                                             >  4:12373/1‑141 (MQ=255)

                                 aCTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTgg                                                                                                             >  4:12469/1‑141 (MQ=255)

                                 aCTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTgg                                                                                                             >  2:354519/1‑141 (MQ=255)

                                 aCTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTgg                                                                                                             >  1:1035589/1‑141 (MQ=255)

                                  cTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGa                                                                                                            >  1:508910/1‑141 (MQ=255)

                                  atAAACAAAATAGACCAAAAAAAATAAAAAAATATAAGCCTACAACACATAACTATAAAACAAATAGTAATAATAAACCTAATTTTGATAAATTAGAAAAAAAAAAAAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGa                                                                                                            <  2:1054861/140‑1 (MQ=255)

                                   tAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGaa                                                                                                           >  3:20206/1‑141 (MQ=255)

                                       cAAAATAGACCGAATTCTATAACAAAATATGATCCAACAAAACACAATTTTAAAGAGAAGAGTGAAAATAAACCTAATTTTGATAAATTAGTTGAAGAAACAAAAAAAGCAGTTAAAGAAGCAGACGAATCTTGGaaaaat                                                                                                       >  2:216027/1‑140 (MQ=255)

                                        aaaaTAGACCGAAATCTATAACGAAATATAATCCTACAACGCATACCTATAAATCAAATAGTGATAATAAAACTAAGTTTGATAAATTAGTTGAAGAAACGAAAAAGGCAGTTATAGTAGCACATGATCCTTGGAAAAAGa                                                                                                      <  2:18772/141‑1 (MQ=255)

                                         aaaTAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGaa                                                                                                     >  2:227268/1‑141 (MQ=255)

                                           aTAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGaaaa                                                                                                   >  2:1005330/1‑141 (MQ=255)

                                            tAGACCGAAATCTATAACGCAATAGAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAANAGAAAAc                                                                                                  >  2:817029/1‑141 (MQ=255)

                                            tAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAAc                                                                                                  >  3:56537/1‑141 (MQ=255)

                                            tAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAAc                                                                                                  >  3:150124/1‑141 (MQ=255)

                                              gACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTg                                                                                                >  2:474560/1‑141 (MQ=255)

                                               aCCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGt                                                                                               >  2:206796/1‑141 (MQ=255)

                                               aCCGAAAACTATAACGAAATATAATCCTAAAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATACTTGGAAAAAGAAAACTGt                                                                                               <  2:841329/141‑1 (MQ=255)

                                                ccGAATTCTATAACAAAATATGATCCAACAAAACACAATTTTAAAGAGAAGAGTGAAAATAAACCTAATTTTGATAAATTAGTTGAAGAAACAAAAAAAGCAGTTAAAGAAGCAGACGAATCTTGGAAAAATAAAACTGTc                                                                                              <  1:805374/141‑1 (MQ=255)

                                                 cGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACGACTGGTGATGATTCGCCTAATTTTGATAAATTAGTTGAGGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCa                                                                                             <  1:487464/141‑1 (MQ=255)

                                                  gAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATGCTAGGAAAAAGAAAACTGTCaa                                                                                            <  1:39066/141‑1 (MQ=255)

                                                     aTCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCaaaaa                                                                                         <  1:934319/141‑1 (MQ=255)

                                                        tataACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATa                                                                                      <  2:265408/141‑1 (MQ=255)

                                                         ataACGAAATATGATCCGAACACGCCTAACTATAAAACAAATACTGATAATAAACCTAATTTTGAATAGTTAGTTCAAGAAACGAAAAGAGCCGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAACCTGTCAAAAAATAc                                                                                     <  4:197403/141‑1 (MQ=255)

                                                         ataACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATAc                                                                                     <  2:1050496/141‑1 (MQ=255)

                                                           aaCGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACgg                                                                                   <  1:118313/141‑1 (MQ=255)

                                                            aCGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTACTTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGga                                                                                  <  2:344919/141‑1 (MQ=255)

                                                            aCGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGga                                                                                  >  4:150667/1‑141 (MQ=255)

                                                            aCGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGga                                                                                  >  4:150655/1‑141 (MQ=255)

                                                            aCGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGga                                                                                  <  3:8959/141‑1 (MQ=255)

                                                             aaaataataATTCTACAAAACATAACTATAAAACAAAGAGTGATAATAAACCTAATTTTGGGAAATTAGTTGAAGAAACGAAAAAAACAGTTAAAGAAGCAGATTATTCTTGGAAAAAGAAAACTGTCAAAAAATACGgag                                                                                 <  2:725299/135‑1 (MQ=255)

                                                              gAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGgaga                                                                                <  3:164557/141‑1 (MQ=255)

                                                                   ataATGCTACAACAGATAACTATAGAACAAATAGTGATAAGAAACCGAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTg                                                                           <  3:246588/141‑1 (MQ=255)

                                                                   ataATCCTACAACACATAACTATAAAACAAATAGTGATAATAAGCCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTg                                                                           <  1:413682/141‑1 (MQ=255)

                                                                    taATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGa                                                                          <  3:292296/141‑1 (MQ=255)

                                                                     aaTCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGaa                                                                         >  1:310390/1‑141 (MQ=255)

                                                                      aTCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGaaa                                                                        <  2:299333/141‑1 (MQ=255)

                                                                        ccTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACa                                                                      <  1:761162/141‑1 (MQ=255)

                                                                             aacaCATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACG‑AAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCg                                                                >  1:773600/1‑141 (MQ=255)

                                                                              acacATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCg                                                                >  4:319316/1‑141 (MQ=255)

                                                                               cacaTAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGc                                                               <  1:74856/141‑1 (MQ=255)

                                                                               cacaTAAATATAAAACACACAGTGATAATAAACCTAATTTTGCTAAATTAGCGGAGTAAACGAAAAACGAAGTTAAAAAAGCAGATGATTCTTGCAAAAAGAAAACTGTCAAAAAATACGAAGAAACTGAAACAAAATCGc                                                               >  4:108543/1‑141 (MQ=255)

                                                                                 caTAACTATAAAACAAATAGTGATAATAAACATAATTTTGATAAATTAGTTGAAGAAACGAACAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCa                                                             <  1:884301/141‑1 (MQ=255)

                                                                                   tAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGCAACGAAAAAAGCAGTTATAGAGGAAGACGATACGTGGGAAGTGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCagt                                                           >  1:213004/1‑141 (MQ=255)

                                                                                   tAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCagt                                                           >  1:439306/1‑141 (MQ=255)

                                                                                   tAACTATAAAACAAAAAGTAAAAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATGCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCagt                                                           <  3:204282/141‑1 (MQ=255)

                                                                                   tAACTAAAAAACAAATCGTGATAATAAACCTCATATTGATAAAATAGATGAAGAAACGAAAAAAGCAATTAAAGAAGCAGATAACTCTTCGAAAAAGAACACTGGCAAAATATACGGATATACTGAAACAAAATCGCCagt                                                           <  2:89078/141‑1 (MQ=255)

                                                                                    aaCTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCagta                                                          <  4:30124/141‑1 (MQ=255)

                                                                                         taAAACAAATAGTGATAATAAACATAATGTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTaaa                                                     >  3:103204/1‑141 (MQ=255)

                                                                                         aaaaaCAAATAATGAAAATAAACCTAATGTTGATAAATTAGTTGAAGAAACGAAAAAAGCAATTAAAGAAGCAGATGATTCTTGGAAAAAAAAAAAAAAAAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTaaa                                                     <  2:517365/140‑1 (MQ=255)

                                                                                           aaaCAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAaag                                                   >  1:429391/1‑141 (MQ=255)

                                                                                             aCAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAaagaa                                                 <  3:135148/141‑1 (MQ=255)

                                                                                                aaTAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAATAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAagaagag                                              >  3:317171/1‑141 (MQ=255)

                                                                                                aaTAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAagaagag                                              <  1:625208/141‑1 (MQ=255)

                                                                                                   aGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAagaagagaag                                           <  3:441933/141‑1 (MQ=255)

                                                                                                   aGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACAAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAagaagagaag                                           <  2:714565/141‑1 (MQ=255)

                                                                                                   aGTGATAATAAACCTAAACTTGATAAATAAAATAAAGAAACGAAAAAAGCAGTTAAAGAAGCAGACAATTCTCGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAACCCCCAGGAGTAAAagaagagaaa                                           >  4:19842/1‑140 (MQ=255)

                                                                                                       ataataAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAg                                       <  1:759652/141‑1 (MQ=255)

                                                                                                       ataataAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAg                                       <  4:258496/141‑1 (MQ=255)

                                                                                                        aaataaACCTAATTTTGATAAATTAGTTGAAAAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGt                                      <  4:236529/140‑1 (MQ=255)

                                                                                                             aaCCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTgaag                                 >  2:123848/1‑141 (MQ=255)

                                                                                                                cTAATTTTGATAAATTAGTTGAAGAAAAGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAAc                              <  2:708970/141‑1 (MQ=255)

                                                                                                                  aaTTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATAGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAAccc                            >  1:344318/1‑140 (MQ=255)

                                                                                                                    ttttGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCa                          >  4:164636/1‑141 (MQ=255)

                                                                                                                       tGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGc                       <  1:838822/141‑1 (MQ=255)

                                                                                                                       tGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGc                       <  1:963619/141‑1 (MQ=255)

                                                                                                                        gATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCa                      <  1:353936/141‑1 (MQ=255)

                                                                                                                         aTAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCAc                     <  3:115075/141‑1 (MQ=255)

                                                                                                                         aTAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCAc                     <  1:156644/141‑1 (MQ=255)

                                                                                                                           aaaTTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCt                   <  4:183025/141‑1 (MQ=255)

                                                                                                                           aaaTTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCt                   <  3:414655/141‑1 (MQ=255)

                                                                                                                            aaTTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTa                  <  1:1055613/141‑1 (MQ=255)

                                                                                                                            aaTTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTa                  <  4:453147/141‑1 (MQ=255)

                                                                                                                                   tGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTAAAGTTGa           >  2:590948/1‑141 (MQ=255)

                                                                                                                                    gaagaaACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTAAAGTTGAt          >  1:544375/1‑141 (MQ=255)

                                                                                                                                      agaaACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTAAAGTTGATaa        >  2:946805/1‑141 (MQ=255)

                                                                                                                                      agaaACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTAAAGTTGATaa        >  1:70423/1‑141 (MQ=255)

                                                                                                                                            gAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTAAAGTTGATAACcaaca  >  1:339235/1‑141 (MQ=255)

                                                                                                                                            gAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTAAAGTTGATAACcaaca  <  1:96397/141‑1 (MQ=255)

TTAAATTCAATTATTGATGATTTCTTTATGGAAACTAAACAAAATAGACCGAAATCTATAACGAAATATAATCCTACAACACATAACTATAAAACAAATAGTGATAATAAACCTAATTTTGATAAATTAGTTGAAGAAACGAAAAAAGCAGTTAAAGAAGCAGATGATTCTTGGAAAAAGAAAACTGTCAAAAAATACGGAGAAACTGAAACAAAATCGCCAGTAGTAAAAGAAGAGAAGAAAGTTGAAGAACCTCAAGCACCTAAAGTTGATAACCAACA  >  USA300TCH1516_ALE/267027‑267307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`