Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 313,483 C→T 5.4% L220L (TTG→TTA rebM ← Demethylrebeccamycin‑D‑glucose O‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE313,4830CT 5.4% 509.3 / 6.7 162L220L (TTG→TTArebMDemethylrebeccamycin‑D‑glucose O‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (72/80);  new base T (7/3);  total (79/83)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 165 total aligned reads displayed.

TCAAACATAGATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAGCCAAG  >  USA300TCH1516_ALE/313343‑313623
                                                                                                                                            |                                                                                                                                            
tCAAACATCGATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATCTCTTTACTCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTc                                                                                                                                              <  1:21011/141‑1 (MQ=255)
 cAAACATAGATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCa                                                                                                                                             <  1:100179/141‑1 (MQ=255)
  aaaCATAGATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCaa                                                                                                                                            <  4:149516/141‑1 (MQ=255)
      aTAGATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCa                                                                                                                                        <  3:243823/141‑1 (MQ=255)
         gATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCAttg                                                                                                                                     >  1:660102/1‑141 (MQ=255)
         gATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCAttg                                                                                                                                     >  1:499500/1‑141 (MQ=255)
          aTACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCAttgt                                                                                                                                    <  1:705141/141‑1 (MQ=255)
           tACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCAttgtt                                                                                                                                   >  1:48263/1‑141 (MQ=255)
               aaGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATa                                                                                                                               <  4:104071/141‑1 (MQ=255)
               aaGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATa                                                                                                                               <  1:668900/141‑1 (MQ=255)
               aaGTACAGTAAGTGGCATTATATTTAGTACCCTTTTTTAGGGACAAAGGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATa                                                                                                                               >  2:38962/1‑141 (MQ=255)
                   aCAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCt                                                                                                                           >  1:125735/1‑141 (MQ=255)
                   aCAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCt                                                                                                                           <  3:423812/141‑1 (MQ=255)
                    cAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCtt                                                                                                                          >  2:907883/1‑141 (MQ=255)
                        tAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTtata                                                                                                                      >  2:331114/1‑141 (MQ=255)
                         aGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAg                                                                                                                     >  2:85640/1‑141 (MQ=255)
                           tGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTTTTTATAATCTTTATAGtt                                                                                                                   >  4:207304/1‑141 (MQ=255)
                           tGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGtt                                                                                                                   <  1:1053820/141‑1 (MQ=255)
                           tGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGtt                                                                                                                   >  1:447800/1‑141 (MQ=255)
                             gCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTcc                                                                                                                 >  3:44891/1‑141 (MQ=255)
                                ttATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCcaac                                                                                                              <  2:201660/141‑1 (MQ=255)
                                ttATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCcaac                                                                                                              >  2:984618/1‑141 (MQ=255)
                                  atatTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCcaacaa                                                                                                            <  4:455561/141‑1 (MQ=255)
                                     tttAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATAc                                                                                                         <  1:1081736/141‑1 (MQ=255)
                                      ttAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATAcc                                                                                                        <  3:294201/141‑1 (MQ=255)
                                       tAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCt                                                                                                       <  2:668239/141‑1 (MQ=255)
                                        aGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCtt                                                                                                      >  3:410519/1‑141 (MQ=255)
                                              ctTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCAt                                                                                                >  1:536877/1‑141 (MQ=255)
                                                tttttAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATaa                                                                                              <  1:985607/141‑1 (MQ=255)
                                                   ttAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAAtt                                                                                           <  4:161636/141‑1 (MQ=255)
                                                    tAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTa                                                                                          <  2:792257/141‑1 (MQ=255)
                                                     aGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTaa                                                                                         >  2:671983/1‑141 (MQ=255)
                                                      gCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTaaa                                                                                        <  1:761099/141‑1 (MQ=255)
                                                       cGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAAc                                                                                       <  1:1040408/141‑1 (MQ=255)
                                                         aCAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCt                                                                                     <  1:56142/141‑1 (MQ=255)
                                                           aaaaGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTTAAAGCATTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCttt                                                                                   <  2:414273/141‑1 (MQ=255)
                                                           aaaaGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCttt                                                                                   <  1:741792/141‑1 (MQ=255)
                                                             aaGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTg                                                                                 >  1:171088/1‑141 (MQ=255)
                                                               gTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGgt                                                                               <  2:587678/141‑1 (MQ=255)
                                                               gTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGgt                                                                               <  4:267236/141‑1 (MQ=255)
                                                                tAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGgtg                                                                              >  1:232628/1‑141 (MQ=255)
                                                                tAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACATTCGTCATAAATTAAACCTTTTGgtg                                                                              >  1:895799/1‑141 (MQ=255)
                                                                   tataATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCa                                                                           <  2:263558/141‑1 (MQ=255)
                                                                     taATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCTAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCAtt                                                                         <  2:934282/141‑1 (MQ=255)
                                                                     taATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAAt                                                                                            <  2:728993/122‑1 (MQ=255)
                                                                     taATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAAt                                                                                            >  1:728993/1‑122 (MQ=255)
                                                                     taATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCAtt                                                                         <  2:888776/141‑1 (MQ=255)
                                                                      aaTTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTa                                                                        <  3:13594/141‑1 (MQ=255)
                                                                         tCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAAc                                                                     <  1:258422/141‑1 (MQ=255)
                                                                            tatCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCa                                                                  >  3:62053/1‑141 (MQ=255)
                                                                               cTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTg                                                               >  2:75298/1‑141 (MQ=255)
                                                                                 ttACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTTAAATCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTTTCATTAAACTCATTGGa                                                             >  4:362365/1‑141 (MQ=255)
                                                                                 ttACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTTATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGa                                                             >  3:89676/1‑141 (MQ=255)
                                                                                 ttACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGa                                                             >  3:13881/1‑141 (MQ=255)
                                                                                    cGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCa                                                          <  2:867502/141‑1 (MQ=255)
                                                                                     gCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAg                                                         <  2:343521/141‑1 (MQ=255)
                                                                                      cAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGt                                                        <  2:461978/141‑1 (MQ=255)
                                                                                         tttAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATg                                                     >  1:371100/1‑141 (MQ=255)
                                                                                           tAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGTACCAGTATGAt                                                   >  1:459904/1‑141 (MQ=255)
                                                                                             gTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTTAAAGCATTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATaa                                                 <  4:202076/141‑1 (MQ=255)
                                                                                              tCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAAt                                                >  1:587662/1‑141 (MQ=255)
                                                                                               cATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATg                                               <  1:1064216/141‑1 (MQ=255)
                                                                                                aTCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTTAAAGCATTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGc                                              >  2:601813/1‑141 (MQ=255)
                                                                                                     tttAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATg                                         >  2:428097/1‑141 (MQ=255)
                                                                                                      ttAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGa                                        <  1:40885/141‑1 (MQ=255)
                                                                                                        aaaCATTTTACAAAACATTGGTCGATTTTCTTTTTTTAAAGCATTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGAtt                                      <  1:613433/141‑1 (MQ=255)
                                                                                                          aCATTTTACAAAACATTGGTCGATTTTCTTTTTTTTTTTTTTTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTaa                                    >  2:833308/1‑141 (MQ=255)
                                                                                                          aCATTTTACAAAACATTGGTCGATTTTCTTTTTTTTTTTTTTTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTaa                                    >  2:484013/1‑141 (MQ=255)
                                                                                                          aCATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTaa                                    <  4:423859/141‑1 (MQ=255)
                                                                                                          aCATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTaa                                    >  4:281794/1‑141 (MQ=255)
                                                                                                            aTTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTTGAACTAAAATCTACCCTTTGGTGTTTTTACACTCATTGGCCAAGTATGATAATGAACATGATTaaaa                                  >  3:268228/1‑141 (MQ=255)
                                                                                                             ttttACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAAc                                 >  2:276663/1‑141 (MQ=255)
                                                                                                             ttttACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTATCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAAc                                 >  2:505114/1‑141 (MQ=255)
                                                                                                              tttACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAAcc                                <  1:298133/141‑1 (MQ=255)
                                                                                                               ttACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACCATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTCGAGCAGTATGATAATGCACATGATTAAAACCa                               >  4:392945/1‑141 (MQ=255)
                                                                                                                tACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAg                              <  4:398524/141‑1 (MQ=255)
                                                                                                                  aaaagCATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGACAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCt                            <  4:402717/140‑1 (MQ=255)
                                                                                                                    aaaCATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTg                          <  2:1078136/141‑1 (MQ=255)
                                                                                                                     aaCATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGa                         >  1:343807/1‑141 (MQ=255)
                                                                                                                     aaCATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGa                         <  1:85640/141‑1 (MQ=255)
                                                                                                                         ttGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATtat                     >  1:520027/1‑141 (MQ=255)
                                                                                                                          ttgTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATtata                    <  2:80483/139‑1 (MQ=255)
                                                                                                                          tGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGAGCAGTATGATAATGCACATGATTAAAACCAGCTTGATtata                    <  2:210226/141‑1 (MQ=255)
                                                                                                                          tGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATtata                    >  1:848720/1‑141 (MQ=255)
                                                                                                                           ggTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATtatat                   >  1:164830/1‑141 (MQ=255)
                                                                                                                            gTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATtatata                  >  1:810296/1‑141 (MQ=255)
                                                                                                                             tCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATaa                 <  2:1018038/141‑1 (MQ=255)
                                                                                                                              cGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATaaa                <  1:770845/141‑1 (MQ=255)
                                                                                                                              cGATTGTCTTTATTCAAGGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGGCATTAAACGCATTGTACCAGTATGATAATGCACATGATTAAAACCAGCTTGAGTATATaaa                <  2:55332/141‑1 (MQ=255)
                                                                                                                                aTTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTCTGATCATGAACTTGCTGAAAACCAGCTTGATTATATAAATc              <  2:48263/141‑1 (MQ=255)
                                                                                                                                 ttttCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCt             >  1:1047417/1‑141 (MQ=255)
                                                                                                                                     cTTTTTTTAAAGCATTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAATCTTTTTGGTGTCATTAAACTCATTGGAGCAGTATGATAATGCACATTATTAAAACCAGCTTTATGATATTAATTTAAca         >  4:306596/1‑140 (MQ=255)
                                                                                                                                     cTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAAcc         <  1:65523/141‑1 (MQ=255)
                                                                                                                                       ttttttAAAGCATTTTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATGATATAAATCTAACCAg       <  2:784346/141‑1 (MQ=255)
                                                                                                                                       tttttCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAATCTTTTGGTGTCATTAAACTCATTGTACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAg       <  4:233186/141‑1 (MQ=255)
                                                                                                                                       tttttCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAg       >  1:581756/1‑141 (MQ=255)
                                                                                                                                       tttttCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAg       <  2:347638/141‑1 (MQ=255)
                                                                                                                                       tttttCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAg       >  1:588603/1‑141 (MQ=255)
                                                                                                                                        ttttCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAGc      >  1:17687/1‑141 (MQ=255)
                                                                                                                                            cAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAGCCAAg  >  3:233085/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCAAACATAGATACAAAGTACAGTTAGTGGCATTATATTTAGTGCTCTTTTTTAGCGACAAAAGTAATATAATTCATATCTTTACGCAATTTAGTCATCGTTTTAAACATTTTACAAAACATTGGTCGATTTTCTTTTTTCAAAGCATTGTTGATAATCTTTATAGTTCCAACAATACCTTCGTCATAAATTAAACCTTTTGGTGTCATTAAACTCATTGGACCAGTATGATAATGCACATGATTAAAACCAGCTTGATTATATAAATCTAACCAGCCAAG  >  USA300TCH1516_ALE/313343‑313623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: