BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	374,866	T→C	5.4%	N196D (AAT→GAT)	USA300HOU_RS01705 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	374,866	0	T	C	5.4%	487.4 / 11.3	162	N196D (AAT→GAT)	USA300HOU_RS01705	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (66/87); new base C (2/7); total (68/94)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

CGTTCAAATGTTGAATGTGGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTTGTGCAATCATTGTTTCGTTTAC  >  USA300TCH1516_ALE/374727‑375004

cgTTCAAATGTTGAATGTGGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTa                                                                                                                                           >  1:207918/1‑141 (MQ=255)

 gTTCAAATGTTGAATGTGGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAg                                                                                                                                          <  4:143385/141‑1 (MQ=255)

  ttCAAATGTTGAATGTGGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGt                                                                                                                                         >  4:184302/1‑141 (MQ=255)

    cAAATGTTGAATGTGGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGtat                                                                                                                                       >  1:726130/1‑141 (MQ=255)

      aaTGTTGAATGTGGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATaa                                                                                                                                     >  1:236194/1‑141 (MQ=255)

           tGAATGTGGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGc                                                                                                                                <  1:862856/141‑1 (MQ=255)

              aTGTGGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAAt                                                                                                                             >  1:963789/1‑141 (MQ=255)

               tgtgGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATg                                                                                                                            <  1:716635/141‑1 (MQ=255)

                gtgGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGt                                                                                                                           >  2:710911/1‑141 (MQ=255)

                  ggAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTa                                                                                                                         <  2:666274/141‑1 (MQ=255)

                   gAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTaa                                                                                                                        <  1:174681/141‑1 (MQ=255)

                        tGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCata                                                                                                                   >  3:426554/1‑141 (MQ=255)

                          aTTAATGATATTTTCGCTTTCGTCAACGGGATCATCGTAATCGCCATTACAATGCGCAATGAAAACAGGTGTAATTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATAtt                                                                                                                 <  2:217230/141‑1 (MQ=255)

                           ttCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATAttt                                                                                                                >  1:281451/1‑141 (MQ=255)

                           ttCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATAttt                                                                                                                <  1:617613/141‑1 (MQ=255)

                           ttCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATAttt                                                                                                                >  2:549584/1‑141 (MQ=255)

                            tCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCCATGAAAGCAGGTCGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTa                                                                                                               <  1:102704/141‑1 (MQ=255)

                              aTGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATc                                                                                                             <  4:324137/141‑1 (MQ=255)

                               tGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATcc                                                                                                            <  2:948058/141‑1 (MQ=255)

                                 atatGTTCGCTTTCCTCAACTGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCAt                                                                                                          <  1:886305/141‑1 (MQ=255)

                                 atatGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATCTTTTGAATTAGTATAATCAGCAATGTTAATAATATTTATCCAt                                                                                                          >  3:440802/1‑141 (MQ=255)

                                  tatGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGGAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCAtt                                                                                                         <  4:457660/141‑1 (MQ=255)

                                  tatGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCAtt                                                                                                         >  2:598770/1‑141 (MQ=255)

                                    tGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTa                                                                                                       >  4:61233/1‑141 (MQ=255)

                                        cGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCtg                                                                                                   >  1:174508/1‑141 (MQ=255)

                                         gCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCtgt                                                                                                  <  2:953705/141‑1 (MQ=255)

                                              ccTCAATGGGGAGATGAGAATCTACATTACAATGCGCAATGAAAACGGGTGGTGGTGTTTTAAGTTTATCTGGTGCTATTTTATATTTTGAATTAGTATAATCAGCAATGTTAATAATATTTATCCATTTACCTGTGCCAc                                                                                             >  2:562698/1‑141 (MQ=255)

                                              catCAACGGGAAAATCATAATCGCCATTACGATCCCCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCAc                                                                                             <  1:753850/139‑1 (MQ=255)

                                               cTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACg                                                                                            <  1:733167/141‑1 (MQ=255)

                                                  aaCGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGc                                                                                         >  4:388556/1‑141 (MQ=255)

                                                  aaCGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGc                                                                                         <  2:343885/141‑1 (MQ=255)

                                                   aCGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCa                                                                                        <  2:330918/141‑1 (MQ=255)

                                                    cGGGAACATCATAATCTCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCAt                                                                                       <  4:403905/141‑1 (MQ=255)

                                                    cGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCAt                                                                                       >  3:418176/1‑141 (MQ=255)

                                                     gggAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATa                                                                                      <  2:467531/141‑1 (MQ=255)

                                                        aaCATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAAc                                                                                   >  2:451310/1‑141 (MQ=255)

                                                        aaCATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAAc                                                                                   >  4:420908/1‑141 (MQ=255)

                                                        aaCATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAAc                                                                                   >  2:998327/1‑141 (MQ=255)

                                                        aaCATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCAATTTACCTGTGCCACGTGCATAAAc                                                                                   >  4:30599/1‑141 (MQ=255)

                                                         aCATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACg                                                                                  >  3:399060/1‑141 (MQ=255)

                                                         aCATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACg                                                                                  >  1:368852/1‑141 (MQ=255)

                                                         aCATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACg                                                                                  >  2:487415/1‑141 (MQ=255)

                                                         aCATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACg                                                                                  >  2:1002477/1‑141 (MQ=255)

                                                         aCATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACg                                                                                  >  1:848938/1‑141 (MQ=255)

                                                          catcatAATCGCCCTTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGt                                                                                 <  3:108169/141‑1 (MQ=255)

                                                          catcatAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGt                                                                                 <  1:569751/141‑1 (MQ=255)

                                                                aaTCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTa                                                                           <  4:243535/141‑1 (MQ=255)

                                                                aaTCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATCAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTa                                                                           <  3:250533/141‑1 (MQ=255)

                                                                   cGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTaaaa                                                                        <  1:287590/141‑1 (MQ=255)

                                                                    gCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGATCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATACATTTACCTATGCCACGTGCATAAACGTAGATTaaaaa                                                                       >  2:369770/1‑141 (MQ=255)

                                                                     ccATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTaaaaaa                                                                      >  2:628191/1‑141 (MQ=255)

                                                                      cATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAAc                                                                     >  1:699764/1‑141 (MQ=255)

                                                                        ttACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCTTTTGGTGCAATATTATATGGTGAAGTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGt                                                                   <  3:268400/141‑1 (MQ=255)

                                                                         tACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGtt                                                                  >  3:95443/1‑141 (MQ=255)

                                                                         tACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGtt                                                                  >  4:36146/1‑141 (MQ=255)

                                                                         tACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGtt                                                                  <  1:927940/141‑1 (MQ=255)

                                                                           cAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTtgt                                                                >  1:129059/1‑141 (MQ=255)

                                                                             aaaagcAATGAAAACAGGAGGAAGAGTTGTAAGTTCATCTGGTGGAAAATTGGATTTTGAATTAGTATAATCAGCAAAGATAATCATATATATCAATTTACCAGTGCCACGTGCATAAACGTAGATTAAAAAACGTGGTGc                                                              >  2:473245/5‑141 (MQ=255)

                                                                              tGCGCAATTAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCg                                                             <  1:593784/141‑1 (MQ=255)

                                                                               gcgcAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGa                                                            <  1:671935/141‑1 (MQ=255)

                                                                                cgcAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGAt                                                           <  2:724326/141‑1 (MQ=255)

                                                                                cgcAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGAt                                                           >  4:117271/1‑141 (MQ=255)

                                                                                 gcAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATCAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAAAGTAGATTAAAAAACGTTGTGCGAtt                                                          <  4:178508/141‑1 (MQ=255)

                                                                                       aaaaCAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCt                                                    <  2:344119/141‑1 (MQ=255)

                                                                                           cAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGaa                                                <  2:117612/141‑1 (MQ=255)

                                                                                              gTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGaacaa                                             <  2:794529/141‑1 (MQ=255)

                                                                                              gTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGaacaa                                             <  4:450486/141‑1 (MQ=255)

                                                                                              gTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTAAATTTTGAATTAGTATAACCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGaacaa                                             <  2:592977/141‑1 (MQ=255)

                                                                                                  aaGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCgg                                         <  3:70714/141‑1 (MQ=255)

                                                                                                  aaGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCgg                                         >  4:148950/1‑141 (MQ=255)

                                                                                                  aaGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATGTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCgg                                         >  2:371552/1‑141 (MQ=255)

                                                                                                  aaGTGTTTTAAGATCATATGGTGCATTAGCATAGTTTGAATCTGTATAATGAGCAATGTTAATCATATTTATTCAGTTACCTGTACCACGTGCATAAACGTAGATTAAAAAACTTTGTGCGATTTGATCTTGAACAACCgg                                         >  2:208701/1‑141 (MQ=255)

                                                                                                   aGTGTTTTAATGTCGTCTCGTGCGATATTATATTTTGAATTCGTATGATCCGCCATGTTAATCATATTTATCCATTTCCCTGTGCCACGTGCATAACGCTAGATTAAAAGACGTTGTGCGATTTGATCTTGAACAACCGgt                                        <  2:876439/141‑1 (MQ=255)

                                                                                                   aGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGgt                                        <  2:113205/141‑1 (MQ=255)

                                                                                                   aGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGgt                                        <  2:16055/141‑1 (MQ=255)

                                                                                                   aGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGgt                                        <  1:602351/141‑1 (MQ=255)

                                                                                                   aGTGTTCTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGgt                                        <  1:835196/141‑1 (MQ=255)

                                                                                                     tgtTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGgtgt                                      <  1:572270/141‑1 (MQ=255)

                                                                                                      gtTTTNAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAANCGTTGTGCGATTTGATCTTGAACAACCGGTGtt                                     <  1:416915/141‑1 (MQ=255)

                                                                                                       ttttAAGTTCATCTGGTGCAATATTATTCTTTGTATTAGTATAATCAGCAGTGTTAATCATATTTATCCATTTACCTGTGCCACGTGGATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTg                                    <  3:404643/141‑1 (MQ=255)

                                                                                                       ttttAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATTCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTg                                    >  2:674068/1‑141 (MQ=255)

                                                                                                        tttAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTgg                                   <  1:298685/141‑1 (MQ=255)

                                                                                                        tttAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTgg                                   <  1:655133/141‑1 (MQ=255)

                                                                                                          tAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAAGCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTg                                 <  2:903482/141‑1 (MQ=255)

                                                                                                            aGTTCATCTGGTGCAATATTATATTTTGAATCAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGaa                               <  4:396475/141‑1 (MQ=255)

                                                                                                             gTTCATCTGGTGTAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAg                              <  2:824934/141‑1 (MQ=255)

                                                                                                             gTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAg                              <  1:468103/141‑1 (MQ=255)

                                                                                                               tCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTg                            >  4:324843/1‑141 (MQ=255)

                                                                                                                cATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATAATATGTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGa                           <  4:440802/141‑1 (MQ=255)

                                                                                                                  tCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGt                         >  1:502892/1‑141 (MQ=255)

                                                                                                                      gTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTtgtg                     <  4:238886/141‑1 (MQ=255)

                                                                                                                           atattatattTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAATAACCGGTGTTGGTGAAGTGAGTTGTGCAATc                >  2:1031074/1‑141 (MQ=255)

                                                                                                                           atattatattTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTTGTGCAATc                >  1:932869/1‑141 (MQ=255)

                                                                                                                               tatattTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTTGTGCAATCATTg            >  3:344235/1‑141 (MQ=255)

                                                                                                                               tatattTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACATGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTTGTGCAATCATTg            >  3:120230/1‑141 (MQ=255)

                                                                                                                                  attTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTTGTGCAATCATTGttt         >  1:653884/1‑141 (MQ=255)

                                                                                                                                  attTTGAATCAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTTGTGCAATCATTGttt         <  3:16034/141‑1 (MQ=255)

                                                                                                                                  attTTGAATCAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTTGTGCAATCATTGttt         <  3:16036/141‑1 (MQ=255)

                                                                                                                                     ttGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTTGTGCAATCATTGTTTCGt      <  4:138944/141‑1 (MQ=255)

                                                                                                                                     ttGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTTGTGCAATCATTGTTTCGt      <  1:85997/141‑1 (MQ=255)

CGTTCAAATGTTGAATGTGGAACATGATTCATGATATGTTCGCTTTCCTCAACGGGAACATCATAATCGCCATTACAATGCGCAATGAAAACAGGTGGAAGTGTTTTAAGTTCATCTGGTGCAATATTATATTTTGAATTAGTATAATCAGCAATGTTAATCATATTTATCCATTTACCTGTGCCACGTGCATAAACGTAGATTAAAAAACGTTGTGCGATTTGATCTTGAACAACCGGTGTTGGTGAAGTGAGTTGTGCAATCATTGTTTCGTTTAC  >  USA300TCH1516_ALE/374727‑375004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`