Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | USA300TCH1516_ALE | 9,716 | T→C | 6.7% | intergenic (+21/+66) | gyrA → / ← nnrD | DNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | USA300TCH1516_ALE | 9,716 | 0 | T | C | 6.7% | 450.3 / 16.8 | 160 | intergenic (+21/+66) | gyrA/nnrD | DNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase |
Reads supporting (aligned to +/- strand): ref base T (75/74); new base C (5/6); total (80/80) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01 |
Only 100 of 174 total aligned reads displayed.
GCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTT > USA300TCH1516_ALE/9576‑9856 | cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGCGGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAACATATACAACAATACTC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCtata < 2:186284/141‑1 (MQ=255) cAATGCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCtata < 2:552855/141‑1 (MQ=255) aaTTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTatat > 1:653850/1‑141 (MQ=255) ttCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGt < 2:476128/141‑1 (MQ=255) aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAgggg < 2:866194/141‑1 (MQ=255) aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAggga < 1:113654/141‑2 (MQ=255) cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATg > 2:1061339/1‑141 (MQ=255) aGTGATTTATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCt < 4:109104/141‑1 (MQ=255) aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAGGGAAGTCt < 1:433007/141‑1 (MQ=255) gTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCtt < 1:211969/141‑1 (MQ=255) tgattgatTCAGAAGAAAATGATTAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTACATATAGGGAAGTCTTa < 4:162995/141‑1 (MQ=255) gattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑ttttt < 2:283433/141‑1 (MQ=255) attCATAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTa < 2:970307/141‑1 (MQ=255) ttCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTAt > 1:177309/1‑141 (MQ=255) cAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGc > 4:327271/1‑141 (MQ=255) agaagaAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAGGGAAGTCTTA‑ttttttatta < 1:618374/141‑6 (MQ=38) aaaaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCATATATGTCGGGGAGTCTTA‑TTTTTATGCTAGaaa > 4:349111/1‑141 (MQ=255) aaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGt < 3:305020/141‑1 (MQ=255) aaTGATGAAGATGGACGTAAGTAATTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGt < 1:283622/141‑1 (MQ=255) atgatgAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTa > 4:27997/1‑141 (MQ=255) tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa < 2:990334/141‑1 (MQ=255) aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTAc < 2:199616/141‑1 (MQ=255) aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTAc < 1:210719/141‑1 (MQ=255) aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTAc < 4:444363/141‑1 (MQ=255) cGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTatat > 1:311117/1‑141 (MQ=255) gTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATAtt < 4:217761/141‑1 (MQ=255) ttGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCaa > 3:47487/1‑141 (MQ=255) tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt < 2:293606/141‑1 (MQ=255) gTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTCTTATGCTAGAAAGTAATGCTTTACTATATTCAATGAtt > 2:489259/1‑141 (MQ=255) tAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATACTCAATGATTa > 2:691956/1‑141 (MQ=255) aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt > 2:455014/1‑141 (MQ=255) aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt < 1:564714/141‑1 (MQ=255) gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa > 1:100784/1‑141 (MQ=255) cAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTGTACTATATTCAATGAt < 1:451709/141‑1 (MQ=255) aaGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATg < 3:327271/141‑1 (MQ=255) aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa < 2:940476/141‑1 (MQ=255) aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa > 2:192083/1‑141 (MQ=255) aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TATTTATGCTAGAAAGTAATGCTTTACTATATTAAATTATTAGTAAGGa > 4:190925/1‑141 (MQ=255) gATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGAc < 1:90786/141‑1 (MQ=255) tttCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTa < 4:444285/141‑1 (MQ=255) tttCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATCACTa > 2:788497/1‑141 (MQ=255) tCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAAc > 1:232149/1‑141 (MQ=255) aTGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACtt < 3:199929/141‑1 (MQ=255) aTGGATCATGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACGATATTCAATGATTAGTAATGACTAACtt > 3:402831/1‑141 (MQ=255) tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt > 2:20686/1‑141 (MQ=255) tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt > 3:122668/1‑141 (MQ=255) tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt > 2:181790/1‑141 (MQ=255) gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt < 3:330685/141‑1 (MQ=255) gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt > 1:1030712/1‑141 (MQ=255) gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt < 2:321510/141‑1 (MQ=255) aTCGTGTTGAAGAAGATATACAACAATCATG‑‑‑‑‑‑AGATGGAGAATAAT‑AAAAAATAAGACTACCCTATATGAAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTa > 2:913163/1‑141 (MQ=255) gtgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt < 1:671668/141‑1 (MQ=255) tgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTg < 1:111178/141‑1 (MQ=255) ggtTGAAGAAGATATACATCACTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTg < 4:352847/140‑1 (MQ=255) gaagaaGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTc < 3:180200/141‑1 (MQ=255) agaagaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCAt > 2:683224/1‑141 (MQ=255) agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc > 4:60491/1‑141 (MQ=255) agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc > 4:336489/1‑141 (MQ=255) acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg < 2:892026/141‑1 (MQ=255) acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg < 1:690230/141‑1 (MQ=255) acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAACAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg < 1:1041746/141‑1 (MQ=255) caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt > 2:393441/1‑141 (MQ=255) caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt > 3:272818/1‑141 (MQ=255) caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCCTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt > 2:499878/1‑141 (MQ=255) caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGGCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt > 4:386869/1‑141 (MQ=255) acaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt < 2:344300/141‑1 (MQ=255) aaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAttt < 1:332201/141‑1 (MQ=255) tcatc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa > 2:666732/1‑141 (MQ=255) tcatc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa > 2:724733/1‑141 (MQ=255) catc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCAt < 3:451077/141‑1 (MQ=255) c‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa > 2:907586/1‑141 (MQ=255) aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt < 2:1010903/141‑1 (MQ=255) aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt > 2:125287/1‑141 (MQ=255) aTGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATCGATaa > 3:68557/1‑141 (MQ=255) aagaaTAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTc < 4:25393/141‑1 (MQ=255) agaaTAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCt < 1:840406/141‑1 (MQ=255) aat‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCTTTGCGTAAGGTATTTCATTGATAGGTCTTGAtg > 1:682025/1‑141 (MQ=255) at‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg > 1:728157/1‑141 (MQ=255) at‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg > 1:728038/1‑141 (MQ=255) aaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtgg < 1:94283/141‑1 (MQ=255) aaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGc < 4:53366/141‑1 (MQ=255) aaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGca < 2:761296/141‑1 (MQ=255) aaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcac < 3:103367/141‑1 (MQ=255) aTAAGACTCCCCTACATATAGGGAAGTCTTATTTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcac > 1:528655/1‑141 (MQ=38) aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCacaaca > 2:804462/1‑141 (MQ=255) aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca > 1:520281/1‑141 (MQ=255) aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca > 2:321883/1‑141 (MQ=255) aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca > 1:244719/1‑141 (MQ=255) aGACTCCCCTACATATAGGGAAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca > 1:451741/1‑141 (MQ=255) gACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacat > 2:455634/1‑141 (MQ=255) actccccTACATATAGGGAAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacata > 3:137706/5‑141 (MQ=255) cTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac < 1:196416/141‑1 (MQ=255) cTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac < 2:635591/141‑1 (MQ=255) tCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat > 1:815935/1‑141 (MQ=255) cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGTCACCAcatacata > 3:408167/1‑141 (MQ=255) cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCAcatacata > 1:257318/1‑141 (MQ=255) atatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt > 1:185172/1‑141 (MQ=255) atatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt > 1:185174/1‑141 (MQ=255) atatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt > 1:708208/1‑141 (MQ=255) tatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCttt < 1:132340/141‑1 (MQ=255) | GCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTT > USA300TCH1516_ALE/9576‑9856 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |