Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | USA300TCH1516_ALE | 9,725 | G→A | 6.0% | intergenic (+30/+57) | gyrA → / ← nnrD | DNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | USA300TCH1516_ALE | 9,725 | 0 | G | A | 6.0% | 521.7 / 14.7 | 168 | intergenic (+30/+57) | gyrA/nnrD | DNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase |
Reads supporting (aligned to +/- strand): ref base G (82/75); new base A (5/5); total (87/81) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01 |
Only 100 of 176 total aligned reads displayed.
ACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAGGT > USA300TCH1516_ALE/9585‑9864 | aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAgggg > 1:997634/1‑141 (MQ=255) cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATTGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGa < 2:296796/141‑1 (MQ=255) gAAGTGAGTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGt < 2:224369/141‑1 (MQ=255) aGTGATTTATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCt < 4:109104/141‑1 (MQ=255) aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAGGGAAGTCt < 1:432935/141‑1 (MQ=255) tgattgatTCAGAAGAAAATGATTAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTACATATAGGGAAGTCTTa < 4:162995/141‑1 (MQ=255) tgattgatTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑‑AAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑t < 1:107740/141‑1 (MQ=255) gattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑ttttt < 2:283433/141‑1 (MQ=255) attCATAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTa < 2:970307/141‑1 (MQ=255) ttCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTAt > 1:177309/1‑141 (MQ=255) cAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGc > 4:327271/1‑141 (MQ=255) agaagaAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAGGGAAGTCTTA‑ttttttatta < 1:618374/141‑6 (MQ=38) aagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGCGATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAAAAAGACTCCTCTCTAGGTCGCGGAGCCTTA‑TTCTTATGCTAg > 1:562203/1‑141 (MQ=255) aaaaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAGGAATAAT‑AAAAAATAAGACTTCCCTACATGTAGGGGAGTCTTA‑TTATTAAGCTAGaaa > 2:794063/1‑141 (MQ=255) aaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGt < 3:305020/141‑1 (MQ=255) tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa < 1:622781/141‑1 (MQ=255) tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa < 4:30878/141‑1 (MQ=255) tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa < 1:210884/141‑1 (MQ=255) tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATA‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa > 1:243254/1‑141 (MQ=255) aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTAc < 2:199616/141‑1 (MQ=255) aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTAc < 4:444363/141‑1 (MQ=255) ggACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACta > 1:192107/1‑141 (MQ=255) ggACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACta > 1:746766/1‑141 (MQ=255) cGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTatat > 1:311117/1‑141 (MQ=255) gTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATAtt < 4:217761/141‑1 (MQ=255) ttGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCaa > 3:47487/1‑141 (MQ=255) tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt < 2:487767/141‑1 (MQ=255) tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt < 2:293606/141‑1 (MQ=255) aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt > 2:455014/1‑141 (MQ=255) gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa > 1:100784/1‑141 (MQ=255) cAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTGTACTATATTCAATGAt < 1:451709/141‑1 (MQ=255) aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa < 2:940476/141‑1 (MQ=255) aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa < 1:521773/141‑1 (MQ=255) aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TATTTATGCTAGAAAGTAATGCTTTACTATATTAAATTATTAGTAAGGa > 4:190925/1‑141 (MQ=255) gATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGAc < 1:90786/141‑1 (MQ=255) tCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAAc > 1:232149/1‑141 (MQ=255) cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt > 3:53366/1‑141 (MQ=255) aTGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACtt < 3:199929/141‑1 (MQ=255) aTGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACtt < 3:374438/141‑1 (MQ=255) tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt > 3:122668/1‑141 (MQ=255) gATCGTGTTGAAGAAGATATACAACAGGCATC‑‑‑‑‑‑AGATGAAGAATGAT‑AAGAAATAAGACTGCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTGACTATATTCAATGATTAGTGATGACTAACTTTCt < 2:997634/141‑1 (MQ=255) gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt < 3:330685/141‑1 (MQ=255) gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt > 1:1030712/1‑141 (MQ=255) aTCGTGTTGAAGAAGATATACAACAATCATG‑‑‑‑‑‑AGATGGAGAATAAT‑AAAAAATAAGACTACCCTATATGAAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTa > 2:913163/1‑141 (MQ=255) gtgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt > 1:127116/1‑141 (MQ=255) gtgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt < 1:671668/141‑1 (MQ=255) ggtTGAAGAAGATATACATCACTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTg < 4:352847/140‑1 (MQ=255) tGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGttt > 1:489802/1‑141 (MQ=255) agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc > 4:60491/1‑141 (MQ=255) agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc > 4:336489/1‑141 (MQ=255) gaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCg < 2:686842/141‑1 (MQ=255) taCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAg > 1:433023/1‑141 (MQ=255) acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg < 2:185885/141‑1 (MQ=255) acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg < 1:271121/141‑1 (MQ=255) acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAACAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg < 1:1041746/141‑1 (MQ=255) caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt > 3:272818/1‑141 (MQ=255) caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt > 2:393441/1‑141 (MQ=255) caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCCTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt > 2:499878/1‑141 (MQ=255) caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGGCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt > 4:386869/1‑141 (MQ=255) acaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt < 1:833678/141‑1 (MQ=255) acaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt < 2:344300/141‑1 (MQ=255) tcatc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa < 2:1026836/141‑1 (MQ=255) tcatc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa > 2:666732/1‑141 (MQ=255) tcatc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa > 2:724733/1‑141 (MQ=255) catc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCAt < 3:451077/141‑1 (MQ=255) c‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa > 2:907586/1‑141 (MQ=255) acaTGAAGAATAAT‑AAATAATAAGACTTCCCCATATGGAGGGCAGTCTTA‑‑TTTGATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAGTGTTTCATTGCGTAAGGTATTTCATTGATa < 3:327034/139‑1 (MQ=255) aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt > 2:125287/1‑141 (MQ=255) aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt < 1:323896/141‑1 (MQ=255) gaagaaTAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGt > 2:1067252/1‑141 (MQ=255) aagaaTAAT‑AAAAAATAAGACGTCCCTATATGTAGTGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTc < 4:415761/141‑1 (MQ=255) agaaTAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCt < 1:840406/141‑1 (MQ=255) aat‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCTTTGCGTAAGGTATTTCATTGATAGGTCTTGAtg > 1:682025/1‑141 (MQ=255) at‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg > 1:728038/1‑141 (MQ=255) at‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg < 2:717268/141‑1 (MQ=255) aaaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtg > 2:576217/1‑141 (MQ=255) aaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtgg < 2:194244/141‑1 (MQ=255) aaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGc < 4:53366/141‑1 (MQ=255) aaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTAATGGTTGca > 2:890190/1‑141 (MQ=255) aaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcac < 3:103367/141‑1 (MQ=255) tAAGACTCCCCTACATATAGGGAAGTCTTATTTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcacc > 3:328910/1‑141 (MQ=38) aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCacaaca > 2:804462/1‑141 (MQ=255) aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca > 2:321883/1‑141 (MQ=255) gACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacat > 2:455634/1‑141 (MQ=255) actccccTACATATAGGGAAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacata > 3:137706/5‑141 (MQ=255) cTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac < 2:635591/141‑1 (MQ=255) tCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat > 1:256462/1‑141 (MQ=255) tCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat > 1:698677/1‑141 (MQ=255) cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGTCACCAcatacata > 3:408167/1‑141 (MQ=255) cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCAcatacata > 1:200707/1‑141 (MQ=255) cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCAcatacata > 4:2626/1‑141 (MQ=255) tacatatAGGGAAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCt > 1:356358/7‑141 (MQ=255) atatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt > 1:185174/1‑141 (MQ=255) atatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt > 1:708208/1‑141 (MQ=255) tatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCttt < 1:132340/141‑1 (MQ=255) tGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTg > 1:182175/1‑141 (MQ=255) gTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGc > 4:203455/1‑141 (MQ=255) gTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGc < 2:770392/141‑1 (MQ=255) ggggAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAg > 2:750261/1‑141 (MQ=255) ggAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAGGt < 2:844236/141‑1 (MQ=255) | ACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGCAAGGT > USA300TCH1516_ALE/9585‑9864 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |