New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 407090 =103 (1.220)4 (0.050) 4/236 NT 4.5% intergenic (+5/+78) USA300HOU_RS01880/kynB putative acetyl‑CoA acyltransferase/Kynurenine formamidase
?USA300TCH1516_ALE 407140 = 77 (1.000)intergenic (+55/+28) USA300HOU_RS01880/kynB putative acetyl‑CoA acyltransferase/Kynurenine formamidase
Rejected: Frequency below/above cutoff threshold.

CTATCAGCCCTAAACCAAAAGATGCACCTGGGTTCCCTGTTAGAGCATTTGCAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATTAATACTGTTGTGATAACCTGTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/407241‑407090
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ataacctgttttTATTTGTTTATGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCACGAGTTGGTAATTGATCTA  >  USA300TCH1516_ALE/407140‑407277
                                                                                                                                                                                                                                                                                                  
CTATCAGCCCTAAACCAAAAGATGCACCTGGGTTCCCTGTTAGAGCATTTGCAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATTAATACTGTTGTGA                                                                                                                                                       <  1:675479/141‑1
CTATCAGACATAAACCAAAGAAGGCACCTGGATTCCCTGTTAGATGATTTGCAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATTAATACTGTTGTGA                                                                                                                                                       <  2:93783/141‑1
      GCCCTAAACCAAAAGATGCACCTGGGTTCCCTGTTAGAGCATTTGCAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATTAATACTGTTGTGATAACCT                                                                                                                                                 <  1:833822/141‑1
      GCCCTAAACCAAAAGATGCACCTGGGTTCCCTGTTAGAGCATTTGCAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATTAATACTGTTGTGATAACCT                                                                                                                                                 >  2:84133/1‑141
       CCCTAAACCAAAAGATGCACGTGGGTTACATTTTAGAGCATTTGAAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATTAAGACTGTTGTGATAACCTG                                                                                                                                                <  2:413248/141‑1
        CCTAAACCAAAAGATGCACCTGGGTTCCCTGTTAGAGCATTTGCAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATTAATACTGTTGTGATAACCTGT                                                                                                                                               <  1:938532/141‑1
        CCTAAACCAAAAGATGCACCTGGGTTCCCTGTTAGAGCATTTGCAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATTAATACTGTTGTGATAACCTGT                                                                                                                                               <  1:965994/141‑1
         CTAAACCAAAAGATGCACCTGGGTTCCCTGTTAGAGCATTTGCAATTAAACCATCAAATGACTAATATTACCCAATATAGCCATAAACAAATAAAATCAGGTTATGCCAACATGTAATTAATACTGTTGTGATAACCTGTT                                                                                                                                              <  2:895557/141‑1
             ACCAAAAGATGCACCTGGGTTCCCTGTTAGAGCATTTGCAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATAAATACTGTTATGATAACCTTTTTTTt                                                                                                                                          >  2:570550/1‑140
                  AAGATGCACCTGGGTTCCCTGTTAGAGCATTTGCAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATTAATACTGTTGTGATAACTTGTTTTTATTTGT                                                                                                                                     >  2:973619/1‑141
                                                              TCAAAAAACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTCTGTTAACAATGAATTAATACTTTTGTGATAACCTGTTTTTATTTGTTTATGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTG                                                                                         <  1:973619/141‑1
                                                                                                        AACAGGTTATGCCAACATGTAATTAATACTGTTGTGATAACCTGTTTTTATTTGTTTATGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAG                                               >  2:390271/1‑141
                                                                                                                                  acaTGTTGTGATAATGTGTTTTGATGTGTTTATGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCA                     <  2:90460/138‑1
                                                                                                                                              AACCTGTTTTTATTTGTTTATGGATTTATTGGGTAATATTCGTCCTATGAGTGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCACGAGTTGGTAAT         <  2:463224/141‑1
                                                                                                                                              AACCTGTTTTTATTTGTTTATGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGATGATAACATTAATCATGTCGTCACGAGTTGGTAAT         >  2:5468/1‑141
                                                                                                                                              AACCTGTTTTTATTTGTATATGTATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCCCGAGTTGtgaat         >  2:34102/1‑136
                                                                                                                                               ACCTGTTTTTATTTGTTTATGGATTTAGTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATCGCGTAAATGATGGCGCCACGAGTTGGTAATT        >  2:632406/1‑141
                                                                                                                                                CCTGTTTTTATTTGTTTATGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCACGAGTTGGTAATTG       >  2:95508/1‑141
                                                                                                                                                   tTTTTGATTTGTTTAGGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCACGAGTTGGTAATTGATC    <  2:1002990/140‑1
                                                                                                                                                   GTTTTTATTTGTTTATGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCACGAGTTGGTAATTGATC    <  1:25468/141‑1
                                                                                                                                                    TTTTTATTTGTTTATGGATTGATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACACAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCACGAGTTGGTAATTGATCT   <  2:201882/141‑1
                                                                                                                                                     TTTTATTTGTTTATGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCACGAGTTGGTAATTGATCTA  >  1:216467/1‑141
                                                                                                                                                     TTTTATTTGTTTATGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCACGAGTTGGTAATTGATCTA  >  1:898728/1‑141
                                                                                                                                                                                                                                                                                                  
CTATCAGCCCTAAACCAAAAGATGCACCTGGGTTCCCTGTTAGAGCATTTGCAATTAAACCATCAAATGACTAATATTACCCAATAAATCCATAAACAAATAAAAACAGGTTATGCCAACATGTAATTAATACTGTTGTGATAACCTGTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/407241‑407090
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ataacctgttttTATTTGTTTATGGATTTATTGGGTAATATTAGTCATTTGATGGTTTAATTGCAAATGCTCTAACAGGGAACCCAGGTGCATCTTTTGGTTTAGGGCTGATAGCGTAAATGATGGCGCCACGAGTTGGTAATTGATCTA  >  USA300TCH1516_ALE/407140‑407277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.