New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE = 145402960 (0.710)3 (0.040) 3/236 NT 4.9% intergenic (‑108/+176) odhA/arlS 2‑oxoglutarate dehydrogenase E1 component/Signal transduction histidine‑protein kinase ArlS
?USA300TCH1516_ALE = 1454035 62 (0.810)intergenic (‑114/+170) odhA/arlS 2‑oxoglutarate dehydrogenase E1 component/Signal transduction histidine‑protein kinase ArlS
Rejected: Frequency below/above cutoff threshold.

TTACAGGAGCCTCTGAAACTTCTTTTCTTTCGTTAGTCATTTTACACCCTCCACAAAAATGTTGAAACGCTTACAATGTAATAATATCACTATTTAGTTATTTGTTAAAGTAGATTTACCTATTTATCTGCTAAAAAGAAGAATCAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/1453882‑1454029
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agaagaatcaacGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGAAAAGAAGTTT  <  USA300TCH1516_ALE/1454035‑1453897
                                                                                                                                                                                                                                                                                               
TTACAGGAGCCTCTGAAACTTCTTTTCTTTCGTTAGTCATTTTACACCCTCAACAAAAATGTTGAAACGCTTACAATGTAATAATATCACTATATAGTTATTTGTTAAAGTAGATTTCCCTATTTATCTTCTAAAAATAAa                                                                                                                                                    >  2:229343/1‑140
  ACAGGAGCCTCTGAAACTTCTTTTCTTTCGTTAGTCATTTTACACCCTCCACAAAAATGTTGAAACGCTTACAATGTAATAATATCACTATTTAGTTATTTGTTAAAGTAGATTTACCTATTTATCTGCTAAAAAGAAGAA                                                                                                                                                  <  1:1011573/141‑1
     gtAGCCTCTGAAACTTCTTTTCTTGCGTTGGTCATTTTACACCCTCCACAAAAATGTTGAAACGCTTACAATGTAATAATATCACTATTTAGTTATTTGTTAAAGTAGATTTACCTATTTATCTGCTAAAAAGAAGAATCA                                                                                                                                               <  2:487604/139‑1
      GAGCCTCTGAAACTTCTTTTCTTTCGTTAGTCATTTTACACCCTCCACAAAAATGTTGAAACGCTTACAATGTAATAATATCACTATTTAGTTATTTGTTAAAGTAGATTTACCTATTTATCTGCTAAAAAGAAGAATCAA                                                                                                                                              <  2:47646/141‑1
      GAGCCTCTGAAACTTCTTTTCTTTCGTTAGTCATTTTACACCCTCCACAAAAATGTTGAAACGCTTACAATGTAATAATATCACTATTTAGTTATTTGTTAAAGTAGATTTACCTATTTATCTGCTAAAAAGAAGAATCAA                                                                                                                                              <  2:253592/141‑1
                                         TTACACCCTCCACAAAAATGTTGAAACGCTTACAATGTAATAATATCACTATTTAGTTATTTGTTAAAGTAGATTTACCTATTTATCTACTAAAAAGAAGAATCAACGCTTTTATTCATTCTTCTTTTTCGCAGATAtcta                                                                                                           >  2:949325/1‑137
                                                        AAATGTTGAAACGCTTACAATGTAATAATATCACTATTTAGTTATTTGTTAAAGTAGATTTACCTATTTATCTGCTACGAAGACGACTCAACAAAAGCGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTA                                                                                            >  1:783766/1‑141
                                                                                 TAATATCACTATTTAGTTATTTGTTAAAGTAGATTTACCTATTTATCTGCTAAAAAGAAGAATCAACAAAAGCGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATCACTAAATAGTGCTATTcn                                                                   >  1:949325/1‑139
                                                                                                                                        AGAAGAATCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGTTGTAAAATGACTAACGAAAca            >  2:1026311/1‑139
                                                                                                                                        AGAAGAATCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGTTGTAAAATGACTAACGAAAGA            >  2:743319/1‑141
                                                                                                                                        AGAAGAATCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGA            >  1:345498/1‑141
                                                                                                                                        AGAAGAATCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGA            <  1:150077/141‑1
                                                                                                                                        AGAAGAATCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAACGATTCAACATATTTGTGGAGGGTGTAAAATGACAAACGAAAGA            >  2:203111/1‑141
                                                                                                                                           AGAATCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGAAAA         <  1:249278/141‑1
                                                                                                                                           AGAATCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGAAAA         <  1:1007584/141‑1
                                                                                                                                             AATCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATTACTAAATAGACCCGTTCTTTCATTTTAAGCTTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGAAAAGA       >  2:221240/1‑141
                                                                                                                                             AATCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGAAAAGA       <  1:255848/141‑1
                                                                                                                                             AATCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTATGTGGAGGGTGTAAAATGACTAACGAAAGAAAAta       >  2:585186/1‑139
                                                                                                                                               TCAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGAAAAGAAG     >  1:179937/1‑141
                                                                                                                                                CAACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGAAAAGAAGT    >  1:69699/1‑141
                                                                                                                                                  ACGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGAAAAGAAGTTT  >  1:790703/1‑141
                                                                                                                                                                                                                                                                                               
TTACAGGAGCCTCTGAAACTTCTTTTCTTTCGTTAGTCATTTTACACCCTCCACAAAAATGTTGAAACGCTTACAATGTAATAATATCACTATTTAGTTATTTGTTAAAGTAGATTTACCTATTTATCTGCTAAAAAGAAGAATCAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/1453882‑1454029
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agaagaatcaacGCTTTTGTTGATTCTTCTTTTTAGCAGATAAATAGGTAAATCTACTTTAACAAATAACTAAATAGTGATATTATTACATTGTAAGCGTTTCAACATTTTTGTGGAGGGTGTAAAATGACTAACGAAAGAAAAGAAGTTT  <  USA300TCH1516_ALE/1454035‑1453897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.