Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 40,898 T→A 5.4% N79I (AAC→ATC)  pbp ← Beta‑lactam‑inducible penicillin‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE40,8980TA 5.4% 215.9 / 2.7 74N79I (AAC→ATC) pbpBeta‑lactam‑inducible penicillin‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (29/41);  new base A (2/2);  total (31/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

TAACTTCCACATACCATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCAATTGCATCAATAGT  >  USA300TCH1516_ALE/40759‑41037
                                                                                                                                           |                                                                                                                                           
tAACTTCCACATACCATCTTCTTTAACAAACTTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGtt                                                                                                                                            <  1:367135/141‑1 (MQ=255)
tAACTTCCACATACCATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGtt                                                                                                                                            <  1:917900/141‑1 (MQ=255)
tAACTTCCACATACCATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGtt                                                                                                                                            <  1:329749/141‑1 (MQ=255)
 aaCTTCCACATACCATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGttt                                                                                                                                           >  1:896933/1‑141 (MQ=255)
 aaCTTCCACATACCATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATAATACCCAAATTTATTTTAATTTTATATTGAGCATAGAAAAACGGAGAATTTTTCGTCACTGTTGTTATTTTACGATCCTGAATGttt                                                                                                                                           >  1:748176/1‑141 (MQ=255)
   cTTCCACATACCATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTCTAACGTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATGCTTTTTTTATTTTACGATCCTCAATGttttt                                                                                                                                         >  1:242602/1‑139 (MQ=255)
      ccACATGCATTCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATc                                                                                                                                      <  2:880697/141‑1 (MQ=255)
      ccACATACCGTCTTCTTTAACATAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATc                                                                                                                                      <  2:543848/141‑1 (MQ=255)
      ccACATACCATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATc                                                                                                                                      <  1:513462/141‑1 (MQ=255)
       cataTACCATCTTCTTTAACAAAATTATATTGAACGTTGCGAGTAATGTTACAGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCt                                                                                                                                     <  2:830212/138‑1 (MQ=255)
          aTACCATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTa                                                                                                                                  <  2:480308/141‑1 (MQ=255)
           tatcATCTTCTTTTACAATATTTAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTaa                                                                                                                                 <  2:287819/138‑1 (MQ=255)
             ccATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGTTACTTTTTTTTTTTTACATTCTTTCATGTTTATATTTTTAAct                                                                                                                               >  2:966351/1‑140 (MQ=255)
             ccATCTTCTTTAACAAAATTAAATTGAACGTTGCGATAAAAGTTACCGTAGTTGCTTTTAATTTTATATTGAGCATCTACTCTTTCTTTTTTTTTAGATACTTTTTTTTTTTTAATTTCCTTATTGTTTATATATTTatct                                                                                                                               >  2:976863/1‑138 (MQ=255)
              cATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGc                                                                                                                              <  1:562397/141‑1 (MQ=255)
               aTCTTCTTTAACTAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTGTTTATTTTTAGATACTTTTTTTATTTTACGAGCCTGAATGTTTATATCTTTAACGcc                                                                                                                             <  2:701014/141‑1 (MQ=255)
                tcttctTTAACAAAATTAAATTGAACGTTTCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCt                                                                                                                            <  1:388718/141‑1 (MQ=255)
                 cttcttTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTTGCTACTTTTTTTATTTTACTCTCTTTACTGTTTATATCTTTATTGCCtt                                                                                                                           >  2:244433/1‑140 (MQ=255)
                   tcttTAACAAAATTAAATTGAACGTTGCGATAAATGTTACCGGAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTaaa                                                                                                                         <  1:323030/141‑1 (MQ=255)
                    cttTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACTATCCTGTATTTTTATATCTTTTACTCTTaaat                                                                                                                        >  2:74018/1‑140 (MQ=255)
                    cttTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTCTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAAc                                                                                                                        >  1:752094/1‑141 (MQ=255)
                      ttAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGCTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACAATCCTTAATGTTTATATATTTCACGCCTATACta                                                                                                                      >  2:741757/1‑141 (MQ=255)
                                  aattGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTTTATACTTTTTTTATTTTACGATTCTGAATGTTTATATCTTTATTTTCTAAACTTTTATACtttttt                                                                                                          >  2:317870/1‑141 (MQ=255)
                                  aattGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGCTGCTGTGTTTTTATATCTTTAACGCCTAAACTATTATATAttttt                                                                                                          >  1:799767/1‑141 (MQ=255)
                                  aattGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATAttttt                                                                                                          <  1:420871/141‑1 (MQ=255)
                                  aattGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATAttttt                                                                                                          >  2:803752/1‑141 (MQ=255)
                                  aattGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGATTATATCTTTAACGCCTAAACTATTATATAttttt                                                                                                          >  1:615262/1‑141 (MQ=255)
                                  aattGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGATTATATCTTTAACGCCTAAACTATTATATAttttt                                                                                                          >  1:174834/1‑141 (MQ=255)
                                       aaCGTTGCGTTCAATGTTTGCGTAGTTTGTTTTAATTTTATATTGTGCATGTACTCGTTTTTTATTTGTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCgg                                                                                                     <  2:748804/141‑1 (MQ=255)
                                          gTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATTTTTATATCTTTAACGCTTAAACTATTATATATTTTTATCGGACg                                                                                                  <  1:470547/141‑1 (MQ=255)
                                          gTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACg                                                                                                  <  1:971112/141‑1 (MQ=255)
                                          gTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACg                                                                                                  <  1:426733/141‑1 (MQ=255)
                                            tGCGATCATTGTTACTGTAGTTTGTTTTAATTTTATATGGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTTCGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGtt                                                                                                <  2:370424/141‑1 (MQ=255)
                                              cGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACTATCCTTAATTTTTATCTTTTTACTGCATATATTATTATATATTTTTCTTGGATGttta                                                                                              >  2:272135/1‑139 (MQ=255)
                                                    aTGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCAtt                                                                                        <  2:969002/141‑1 (MQ=255)
                                                            gTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTc                                                                                <  1:440949/141‑1 (MQ=255)
                                                              aGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCTTTAATGTTTATATCTTTAACGCCTAAATTATTATATTTTTTTATCGTACTTTCCTTCATTTTTACTTCAc                                                                              >  2:152483/1‑141 (MQ=255)
                                                              aGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGCTCAGTCATTTCTACTTCAc                                                                              >  1:576724/1‑141 (MQ=255)
                                                                         aTTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGTACGTTCAGTCATTTCTACTTCACCTTTATCTCtt                                                                   >  2:441942/1‑141 (MQ=255)
                                                                         aTTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCtt                                                                   >  1:721411/1‑141 (MQ=255)
                                                                          ttttATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTCATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCttt                                                                  >  1:249701/1‑141 (MQ=255)
                                                                             tataTTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAg                                                               >  2:379103/1‑141 (MQ=255)
                                                                              atatTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTTTCGCttttttt                                                              >  2:53560/1‑138 (MQ=255)
                                                                                     gCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTTAATGTTTATATCTTTAAAGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTACTACTTCACCATTATCGCTTTTAGAAATATaa                                                       <  2:908108/141‑1 (MQ=255)
                                                                                     gCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTACCTATGTTTAGCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATaa                                                       <  1:654095/141‑1 (MQ=255)
                                                                                     gCATCTACTCGATTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATaa                                                       <  1:130597/141‑1 (MQ=255)
                                                                                       aTCTACTCGTTTTTTATTTTTAGATACGTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTCCACCATTATCGCTTTTAGAAATATAACt                                                     <  1:466026/141‑1 (MQ=255)
                                                                                        tCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATCTATTTTTATCGGTCGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGACATATAATTg                                                    >  1:285733/1‑141 (MQ=255)
                                                                                        tCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACtt                                                    >  1:520349/1‑140 (MQ=255)
                                                                                          tACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCt                                                  <  2:27867/141‑1 (MQ=255)
                                                                                            cTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTAt                                                >  1:445835/1‑141 (MQ=255)
                                                                                                   tttatttttaGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTtata                                         <  1:317870/141‑1 (MQ=255)
                                                                                                   tttatttttaGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTtata                                         <  1:803752/141‑1 (MQ=255)
                                                                                                   tttatttttaGATACTTTTTTTATTTTACGATCCTGAATGATTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTtata                                         <  2:615262/141‑1 (MQ=255)
                                                                                                   tttatttttaGATACTTTTTTTATTTTACGATCCTGAATGATTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCAGTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTtata                                         <  2:174834/141‑1 (MQ=255)
                                                                                                                 cTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAAt                           >  1:876547/1‑141 (MQ=255)
                                                                                                                    tttttATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAAtttt                        <  2:695704/141‑1 (MQ=255)
                                                                                                                    tttttATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTATATCAAAAGTTCAGTCATTTATACCTCACCATTATCACTTTTAAAAATATAACTATTATATTTATAAACTTGTTTGAAAtttt                        >  2:123264/1‑141 (MQ=255)
                                                                                                                       ttattttaCGATCCTTATTGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTTCTTCTCCATTATCGCTTTTAGATATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTAt                     <  2:369630/141‑1 (MQ=255)
                                                                                                                         attttcCGATCCTGTCTGTATGTATTTTTAACGCTTAAACTATTATATATTTTTATTGTACTTTCAGTCTTTTCTACTTCACTATTATCGCTTTTATAAATATACATGTTATCTTTATATACTTGTTTTAAATTTTTTTCt                   >  2:633646/1‑141 (MQ=255)
                                                                                                                         attttaTGATTCTGAATGTTTATATCTTTATCTCCTAAACTATTATATATTTTTTTCGGACTTTTAGTTTTTTCTTCTTCACCATTATTTCTTTTAAAAATATAACTCTTTTTTTTATAAACTTGTTTTAAAttttttttt                   >  2:158463/1‑137 (MQ=255)
                                                                                                                         attttaCGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCt                   >  1:900769/1‑141 (MQ=255)
                                                                                                                         attttaCGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCt                   >  1:857303/1‑141 (MQ=255)
                                                                                                                          tttttttaTCCTGAATGTTTATATCTTTAACGCCTTAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTTTTTTTTTTCTCTTTTATTAATATAACTGCTTTTTTTATAATCTTTTTTAAAATTTTTAtttt                  >  2:823602/8‑141 (MQ=255)
                                                                                                                          ttttaCGATCCTTAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCtt                  <  1:367166/141‑1 (MQ=255)
                                                                                                                          ttttaCGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTTGAAAATAAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCtt                  >  1:702051/1‑141 (MQ=255)
                                                                                                                           tttaCGATCCTTAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTc                 <  1:436000/141‑1 (MQ=255)
                                                                                                                           tttaCGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTc                 <  1:128002/141‑1 (MQ=255)
                                                                                                                           tttaCGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTc                 <  1:822603/141‑1 (MQ=255)
                                                                                                                           tttaCGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTc                 >  1:67378/1‑141 (MQ=255)
                                                                                                                             taCGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCAGGTGGACTACCGCCTGTTCTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCaa               <  1:752359/141‑1 (MQ=255)
                                                                                                                              aCGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATGAACTTGTTTGAAATTTTTATCTTCAAt              <  1:261095/141‑1 (MQ=255)
                                                                                                                               cggtCCTGAATGTGTATATCTTTAACGCCTAAACTATTATATCTTTGCAGACGACGTTCATTCATTTCTACTTCACCATTATCGCTTTGAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCAAtt             <  1:441942/138‑1 (MQ=255)
                                                                                                                               cGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCAAtt             <  2:721411/141‑1 (MQ=255)
                                                                                                                                gATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCAATTg            <  1:379103/141‑1 (MQ=255)
                                                                                                                                     tGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCAATTGCATCa       <  2:799767/141‑1 (MQ=255)
                                                                                                                                       aaTGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCAATTGCATCAAt     <  1:185742/141‑1 (MQ=255)
                                                                                                                                       aaTGTTTATATCTTTAACCCCCAAACCATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCCTTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCAATTGCATCAAt     <  1:249641/141‑1 (MQ=255)
                                                                                                                                          gTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCAATTGCATCAATAGt  <  1:884548/141‑1 (MQ=255)
                                                                                                                                          gTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCAATTGCATCAATAGt  <  1:604371/141‑1 (MQ=255)
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TAACTTCCACATACCATCTTCTTTAACAAAATTAAATTGAACGTTGCGATCAATGTTACCGTAGTTTGTTTTAATTTTATATTGAGCATCTACTCGTTTTTTATTTTTAGATACTTTTTTTATTTTACGATCCTGAATGTTTATATCTTTAACGCCTAAACTATTATATATTTTTATCGGACGTTCAGTCATTTCTACTTCACCATTATCGCTTTTAGAAATATAACTGCTATCTTTATAAACTTGTTTGAAATTTTTATCTTCAATTGCATCAATAGT  >  USA300TCH1516_ALE/40759‑41037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: