BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,512	A→G	29.9%	S194S (TCA→TCG)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,512	0	A	G	29.9%	161.8 / 77.0	113	S194S (TCA→TCG)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base A (39/40); new base G (14/20); total (53/60)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAA  >  USA300TCH1516_ALE/111373‑111651

cTACTGAGGAAGTTAAATAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGATATATATCATATAATCCAGAGGTGCCGAGTTATTCAg                                                                                                                                             <  1:599462/141‑1 (MQ=255)

cTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAg                                                                                                                                             <  1:323559/141‑1 (MQ=255)

cTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTACCGAGTTATTCAg                                                                                                                                             >  2:77691/1‑141 (MQ=255)

ataattaGGAAGTTAAAGAATAAAACATAAAAAAAGAAATCTAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAg                                                                                                                                             <  2:242550/135‑1 (MQ=255)

  acggAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAAATTTTGGTTTTTTGCGCAATCTAGCACTTTTAAAGATTTAATGAATTATAAAGATGGAGAGATATCATATAATCCAGAGGTGCCGAGTTATGCAGCt                                                                                                                                           >  1:288343/4‑141 (MQ=21)

  aCTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCt                                                                                                                                           >  1:612850/1‑141 (MQ=255)

   cTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                          <  1:541191/141‑1 (MQ=255)

   cTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCAAGAGGTGCCGAGTTATTCGGCta                                                                                                                                          >  2:594603/1‑140 (MQ=255)

   cTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAAACACAGACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                          <  1:33580/141‑1 (MQ=255)

      cgacaGTAAAAGAACAAACCACAAACAAAGAAATCGAACACTTTAAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                                       <  1:41920/137‑1 (MQ=21)

      aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                       >  2:424016/1‑141 (MQ=255)

      aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAat                                                                                                                                       <  2:312389/141‑1 (MQ=255)

      aGGAAGTTAAAGAAGAAAACAGAACAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                       <  1:436026/141‑1 (MQ=255)

       ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                                      <  1:629315/141‑1 (MQ=255)

         aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATc                                                                                                                                    >  2:427520/1‑141 (MQ=255)

          aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                                                                   <  1:490653/141‑1 (MQ=255)

          aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                                                                   >  1:397607/1‑141 (MQ=255)

             tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                                                <  1:517367/141‑1 (MQ=255)

             tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAAtt                                                                                                                                >  2:396454/1‑141 (MQ=255)

               aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                                              >  1:514903/1‑141 (MQ=255)

                agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                             <  1:740854/141‑1 (MQ=255)

                agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                             <  2:290665/141‑1 (MQ=255)

                agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                             <  2:398654/141‑1 (MQ=255)

                 gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATGTTGCAAGGTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                                                            >  1:39164/1‑141 (MQ=17)

                 gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCTAGTGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                            >  1:33247/1‑141 (MQ=255)

                 gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                            <  2:717386/141‑1 (MQ=255)

                 gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                            >  2:901790/1‑141 (MQ=255)

                 aaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAAGTATAAAGATCGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                            <  2:522262/140‑1 (MQ=255)

                    gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATCTCAATTAACTAat                                                                                                                         >  2:661471/1‑141 (MQ=255)

                    gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                         >  1:936351/1‑141 (MQ=255)

                    gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                         >  1:729239/1‑141 (MQ=255)

                    gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                         >  1:300046/1‑141 (MQ=255)

                    gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATAATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                         >  2:902032/1‑141 (MQ=255)

                     aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatg                                                                                                                        <  1:265435/141‑1 (MQ=255)

                      aaaCAAAAAAAAAGAAATCGAAAACTTGAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatga                                                                                                                       <  1:180912/141‑1 (MQ=255)

                        aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGTTGCCGAGTTATTCAGCTCAATAACAATTAACTAatcata                                                                                                                     >  2:860217/1‑140 (MQ=255)

                         cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                                                    <  1:839387/141‑1 (MQ=255)

                         cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                                                    <  1:55206/141‑1 (MQ=255)

                          aTAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                                                                   >  2:100801/1‑141 (MQ=255)

                          aTAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTAGGCTAAATATCAATTAACTAATgatgat                                                                                                                   >  1:986670/1‑141 (MQ=255)

                           tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGATGCCGAGTTATTCGGCTAAATATCAATTAACTACTGATGAtt                                                                                                                  >  2:873793/1‑141 (MQ=255)

                             aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                                <  2:150073/141‑1 (MQ=255)

                                 aaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCTAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATg                                                                                                            <  1:178218/141‑1 (MQ=255)

                                      aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                       >  2:1025003/1‑141 (MQ=255)

                                       tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAc                                                                                                      >  1:232280/1‑141 (MQ=255)

                                            aaCTTTAAGTTTGTTTCCCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGAGGGATATATATCATATAATCCAGAGGTGCCGTGTTATTCATCTCAATATCAATGATCTAATGATGATTATAATGTAAAAC‑AATTa                                                                                                <  1:164564/141‑1 (MQ=18)

                                               tttAAGTTTTTTGCGTAATATAGTAATTTTAAAGAGTTAATGAATTATAAAGATAGAGATATATAATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACgc                                                                                             <  2:63454/141‑2 (MQ=17)

                                               tttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACgc                                                                                             <  1:646460/141‑2 (MQ=255)

                                               tttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACgc                                                                                             <  2:234357/141‑2 (MQ=255)

                                               tttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACgc                                                                                             <  2:487453/141‑2 (MQ=255)

                                               tttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGt                                                                                             <  1:378968/141‑1 (MQ=255)

                                                ttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACgca                                                                                            >  1:744428/1‑139 (MQ=255)

                                                ttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACgca                                                                                            <  2:590932/141‑3 (MQ=255)

                                                 tAAGTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCGGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCaa                                                                                           <  1:1003382/141‑1 (MQ=17)

                                                  aaGGTTGTTGCGCAATATTGCAATTTTAAAGAGTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGTCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCaaa                                                                                          <  2:856571/141‑1 (MQ=14)

                                                   aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCaaaa                                                                                         <  1:579238/141‑1 (MQ=255)

                                                   aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCaaaa                                                                                         <  1:596124/141‑1 (MQ=255)

                                                    gTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAg                                                                                        <  2:1021573/141‑1 (MQ=255)

                                                    gTTTTTTGCACAATATAGAAATTTGAAAGATTTAATGAAGTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAg                                                                                        <  2:364046/141‑1 (MQ=255)

                                                        tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCAAAAGatat                                                                                    >  1:947704/1‑141 (MQ=255)

                                                        tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATAATATAATCCAGAGGTTCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAA‑AAATTTGTAAAatatat                                                                                    >  2:614564/1‑141 (MQ=21)

                                                        tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATAATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAT‑TAAAACTGTTTACGTACAAGctat                                                                                    >  2:407754/1‑141 (MQ=21)

                                                          tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTATCTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGa                                                                                  <  1:396454/141‑1 (MQ=255)

                                                             gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAACGATATGACAt                                                                               >  2:78952/1‑141 (MQ=255)

                                                              cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATa                                                                              <  1:510442/141‑1 (MQ=255)

                                                                    aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACcaaca                                                                        >  2:772288/1‑141 (MQ=255)

                                                                    aGCAATTATAAAGATTTAATGAGTTATAAAGATGGAGTTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCAAAAGATATGATATACcgacg                                                                        <  2:484868/141‑2 (MQ=11)

                                                                     gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGCAAAAGATATGATATAccgacga                                                                       >  1:471970/1‑136 (MQ=11)

                                                                     gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACcaacaa                                                                       >  2:25050/1‑141 (MQ=255)

                                                                      aaaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACaaa                                                                      <  1:104187/140‑1 (MQ=255)

                                                                          tttAAAGATTTAATGAATTATAACGATGTATATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATaaa                                                                  <  1:645425/141‑1 (MQ=255)

                                                                          tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATaaa                                                                  <  1:826851/141‑1 (MQ=255)

                                                                          tttAAAGATTTAATGAAGTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATaaa                                                                  <  1:918191/141‑1 (MQ=255)

                                                                               aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTACTACAGCATCAAATATAATAC‑AATTACGTAAACGATATGACATACCAACACATACAGCAcc                                                             >  2:114892/1‑141 (MQ=17)

                                                                                  tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                          <  1:106147/141‑1 (MQ=255)

                                                                                  tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                          <  1:1016730/141‑1 (MQ=255)

                                                                                  tttAATGAATTATAAAGATGGAGATAGATCATATAATCCAGAGGTGCCGAGTTCTCCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                          <  1:751277/141‑1 (MQ=255)

                                                                                    tAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACAAACAAATAAAACGCAGAAGc                                                        >  2:739295/1‑141 (MQ=255)

                                                                                      aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                      <  1:219192/141‑1 (MQ=255)

                                                                                      aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                      >  1:35441/1‑141 (MQ=255)

                                                                                         aagtataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                   <  2:902894/138‑1 (MQ=255)

                                                                                          aTTATAAAGATGGAGATATATCATATAATCCAGCAATGACGAGTTATTCAGCTCAATATCAATTAAATAATATTGATGATAATGTCCCTT‑TATGACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAACtgttga                                                  >  1:916196/1‑140 (MQ=17)

                                                                                              taAATATGGAGATATTTTATATAATCTAGATGTTTCTAGTTCTACAGTTGGTTATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaa                                              <  2:728163/141‑1 (MQ=14)

                                                                                                 aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                           <  1:635123/141‑1 (MQ=255)

                                                                                                 aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                           <  1:491273/141‑1 (MQ=255)

                                                                                                  gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgga                                          >  1:742511/1‑140 (MQ=255)

                                                                                                  gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                          >  1:266763/1‑141 (MQ=255)

                                                                                                       agaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                     <  1:336444/141‑1 (MQ=255)

                                                                                                        aatatagCAGATAATGCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCTCCGAAGCTGTTGTTGAAAGGTACAgtg                                    <  2:863951/140‑3 (MQ=14)

                                                                                                                    aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                        <  1:77691/141‑1 (MQ=255)

                                                                                                                     aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAACGCCGAAGCTGTTGTTGAAAGGTACAGGAACTTACAAAGGtt                       >  2:585352/1‑141 (MQ=17)

                                                                                                                      tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAACTGTTGTTAAAAAGTACAAGGAATTTAAAAGGTtc                      >  2:235919/1‑141 (MQ=255)

                                                                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTACTGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCGGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                     >  1:25045/1‑141 (MQ=255)

                                                                                                                       ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                     >  1:245986/1‑141 (MQ=255)

                                                                                                                       ccAGAGGTGCCGAGATATTCAGCTGAATATCAATGAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                     <  1:106177/141‑1 (MQ=255)

                                                                                                                         agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATAACAACAAATAAAGCGCCGAAGCTGTTGTTGCAAGGTATAGGGAATTTAACAGGTtcata                   >  1:278185/1‑140 (MQ=255)

                                                                                                                          gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑ACTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGCACAGGGAATTTCAAAGGTtcatca                  >  2:55705/1‑141 (MQ=255)

                                                                                                                              ggacGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTg              <  2:385783/138‑1 (MQ=255)

                                                                                                                                         tCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGAGAATTTAAAAGGTTCATCAGTTGGATATaaaaaa   >  1:163722/1‑141 (MQ=255)

                                                                                                                                          cAGCTCAATATCAATTAACTAATGCTGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGACTTTAAACGGTTCATCAGTTGGATATaacaaaa  >  1:179494/1‑141 (MQ=255)

CTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAC‑AATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAA  >  USA300TCH1516_ALE/111373‑111651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`