Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	100.0%	64.7 / NA	23	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/13);  total (10/13)

CACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATG  >  USA300TCH1516_ALE/1646058‑1646308                                                                                                                                    |                                                                                                                        cACTTGCATCACCCTTTGGTACTGCACCTCTGTGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaa                                                                                                                <  1:100722/141‑1 (MQ=255) cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCACTCATCCCTCCTTTTacaa                                                                                                                >  2:33513/1‑141 (MQ=255)        aacaacATGTGGTAATGCACCTCTATGACCAGATGCTACAATAGTATCGAATGATGTTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTATAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaa                                                                                                         <  2:26813/141‑1 (MQ=255)         acacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                        >  2:186194/1‑141 (MQ=255)             cATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatat                                                                                                    >  1:110117/1‑141 (MQ=255)                   gTAATGCAACTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTTCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                              <  2:202735/141‑1 (MQ=255)                                         tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgc                                                                        <  2:175842/141‑1 (MQ=255)                                                aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                 >  1:220481/1‑141 (MQ=255)                                                            gatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCa                                                     <  1:205172/141‑1 (MQ=255)                                                                      cTGCTCCTAATTCTAGCATTTTGTTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAg                                           <  2:124851/141‑1 (MQ=255)                                                                         cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                        <  2:110117/141‑1 (MQ=255)                                                                                ttCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTCTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGGTGCTTTTTGAATTGAAGCACTTTCGTCAGCGTCTTTGAc                                 >  1:138406/1‑141 (MQ=255)                                                                                   tAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtc                              <  1:186194/141‑1 (MQ=255)                                                                                     gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                            >  1:108772/1‑141 (MQ=255)                                                                                           tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAttt                      <  1:210606/141‑1 (MQ=255)                                                                                             cTTTCTGATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTa                    <  2:160970/141‑1 (MQ=255)                                                                                                       ttGCCTTTAATTCTTTTTCAGTCATTCCTCCTTTTACAACAGTTAAATTATATTCATATGTTTTATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTAAGCATCTTTGACTTCTCTAATCTTATCTACAGTAt          >  2:109086/1‑141 (MQ=255)                                                                                                       ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAACATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAATCAATTTCGTCAGCATCTTTGTCGTCTCTATTTTTCTCTTCAGTAt          >  2:12712/1‑141 (MQ=255)                                                                                                       ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACCATATTAGCTGCTTTTTGAATTAAAGAAATTTCTTCAGCATCTTTAACTTCTTTAATTTTATCTACAGTAt          >  2:191943/1‑141 (MQ=255)                                                                                                       ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACTTCTCTAATTTTATCTACAGTAt          >  2:201734/1‑141 (MQ=255)                                                                                                            tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACCAAAATAATATTTTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaa     <  1:101771/141‑1 (MQ=255)                                                                                                               aaTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg  <  1:44847/141‑1 (MQ=255)                                                                                                               aaTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg  <  1:168840/141‑1 (MQ=255)                                                                                                                                    |                                                                                                                        CACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATG  >  USA300TCH1516_ALE/1646058‑1646308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑