Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000731 25,663 Δ1 bp 100% coding (316/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073125,6630A.96.1% 323.5 / 2.4 76coding (316/333 nt)USA300HOU_pUSA300HOUMR0031hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base . (46/27);  total (46/30)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACA  >  CP000731/25524‑25797
                                                                                                                                           |                                                                                                                                      
ttgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaa                                                                                                                                       >  1:265436/1‑141 (MQ=255)
  gtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaa                                                                                                                                     <  1:943/141‑1 (MQ=255)
  gtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaa                                                                                                                                     >  2:231305/1‑141 (MQ=255)
     tGTTATTTACACTAGTGCTTAAATTTACAGGTCTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCTGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGATTAATTACTATACTACAAATaaaaaat                                                                                                                                  >  2:259783/1‑140 (MQ=255)
       ttATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatat                                                                                                                                >  1:260432/1‑138 (MQ=255)
        tATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatata                                                                                                                               <  1:219510/141‑5 (MQ=255)
              cacTAGTTCGTAAATTTACAGGTTTTTCACAAAGTAAAGAAGGATCTTGGTTTTCGTAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTa                                                                                                                        <  2:221592/141‑1 (MQ=255)
              cacTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTa                                                                                                                        >  2:97647/1‑141 (MQ=255)
              cacTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCAATACACCATAATCTGTTTAATTACTATACTACAAATAAAAAATATACATCTa                                                                                                                        >  1:125588/1‑141 (MQ=255)
               acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaa                                                                                                                       <  2:293375/141‑1 (MQ=255)
                cTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaaa                                                                                                                      <  1:222548/141‑1 (MQ=255)
                   gTGCTTAAATTTAGAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGt                                                                                                                   <  2:138691/141‑1 (MQ=255)
                   gTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGt                                                                                                                   >  1:56592/1‑141 (MQ=255)
                   gTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGt                                                                                                                   >  1:31584/1‑141 (MQ=255)
                       atAAATCTAGAGCTATGTAACAAAGTATTGAAGGATCTTGTTTTGCTCAATATCCAGAAATTTTCTTTATAATTCTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTaaa                                                                                                               <  2:30919/140‑1 (MQ=255)
                        tAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAAt                                                                                                              >  1:166778/1‑141 (MQ=255)
                           aTTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTaa                                                                                                           <  1:157456/141‑1 (MQ=255)
                             ttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATg                                                                                                         >  2:97760/1‑141 (MQ=255)
                                cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTGTGCTGTTTAATTACTATACTACAAATGAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                      >  1:192245/1‑141 (MQ=255)
                                cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATTCTACAAATAAAAAATATACATCTAATGTTTAAATTAATGaaa                                                                                                      >  2:289787/1‑141 (MQ=255)
                                cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTATTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTACTGaaa                                                                                                      >  2:149582/1‑141 (MQ=255)
                                   gTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAAt                                                                                                   <  1:28663/141‑1 (MQ=255)
                                        tCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAAt                                                                                              <  1:145334/141‑1 (MQ=255)
                                         cACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTATTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATTTAAAGGTTAAATTGATGAAAATCAGAAtt                                                                                             >  1:60608/1‑141 (MQ=255)
                                          aCCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTAAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTa                                                                                            >  1:243792/1‑141 (MQ=255)
                                                    gAATGATCTTGGTTTACTCAATATCCAGAAATTTTCTTTATAATTGTAGAACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAg                                                                                  <  1:40076/141‑1 (MQ=255)
                                                     aaGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAATGTTAAATTAATGAAAAATAGAATTAGAAAATATAGa                                                                                 >  2:200832/1‑141 (MQ=255)
                                                     aaGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGa                                                                                 >  1:211296/1‑141 (MQ=255)
                                                      aGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGaa                                                                                >  1:268432/1‑141 (MQ=255)
                                                      aGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGaa                                                                                >  1:268435/1‑141 (MQ=255)
                                                         aTCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAAc                                                                             >  1:171844/1‑141 (MQ=255)
                                                            ttGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaa                                                                          >  2:236068/1‑141 (MQ=255)
                                                              tgTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                        <  1:32990/140‑1 (MQ=255)
                                                              ggTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                        >  2:78872/1‑141 (MQ=255)
                                                              ggTTTTCTCAATATCCAGAAAATTTCTTTATAAATATAAAACTAATTATTATGCTGTTTAATTACTATAATACAAATAAAAAATATAAATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                        <  2:43956/141‑1 (MQ=255)
                                                               gTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCAATATTTCTTATGCTGTTTAAGTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAg                                                                       <  2:264768/141‑1 (MQ=255)
                                                                 tttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGa                                                                     >  2:39175/1‑141 (MQ=255)
                                                                 tttCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGa                                                                     <  1:189179/141‑1 (MQ=255)
                                                                 tttCTCAATATCAAGAAATTTTATTTATAATTGTAGCAATATTTCTTATACTGTTTAATTAATATACTACTAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGa                                                                     <  2:201400/141‑1 (MQ=255)
                                                                 tttCTCAATATCAAATAATTTTGTTGATCATTCCATGACGGTTTCTTATGATGATAAATTATTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGa                                                                     <  2:131375/141‑1 (MQ=255)
                                                                   tctcAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGCATATAGAAAACAAAAAGGAAt                                                                   >  1:183521/1‑141 (MQ=255)
                                                                     tcAATATCCAGAAATTTTATTTATAATTGTAGCACTAATTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAAttt                                                                 <  1:181559/141‑1 (MQ=255)
                                                                        atatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtcta                                                              >  1:284919/1‑140 (MQ=255)
                                                                         tatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCa                                                             >  2:263079/1‑141 (MQ=255)
                                                                         tatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCa                                                             >  2:9341/1‑141 (MQ=255)
                                                                         tatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATATTGTTTAATTACTATACTACAACTAAAAAATATACATCTAAAGGTTAAATTATTGAAAAATAGAATAATAGAATATAGAAAACAAAAAGGAATTTCTCa                                                             >  2:62574/1‑141 (MQ=255)
                                                                           tCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                           <  1:260231/141‑1 (MQ=255)
                                                                           tCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                           <  1:48888/141‑1 (MQ=255)
                                                                            acAGAAAGTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGa                                                          <  2:238440/140‑1 (MQ=255)
                                                                             cAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACACAAAGGAATTTCTCAAGaa                                                         >  1:85680/1‑141 (MQ=255)
                                                                              aGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAACTAAAAAATATAAATCTAAAGGTTAAATTAATGAAAAATAAAATTAGAAATTATATACAACAAAAAGCAATCTCCCAAGaaa                                                        >  2:118785/1‑141 (MQ=255)
                                                                              aGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGaaa                                                        >  2:13414/1‑141 (MQ=255)
                                                                               gAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGaaaa                                                       >  2:215214/1‑140 (MQ=255)
                                                                                 aaTTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTCATTACTATACTACAACTAAAAACTATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACtt                                                     >  1:147681/1‑141 (MQ=255)
                                                                                    tttCATTAAAAATATAGAAATAATTATTATGATGTTTAATTAATAAAAAAAAAAAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATAAAGAAAACAAAAAGGAATTTCTCAAGAAACTTTaa                                                  <  2:211296/141‑1 (MQ=255)
                                                                                      tCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGACTTTCTCAAGAAACTTTAAGt                                                >  2:174001/1‑141 (MQ=255)
                                                                                      tCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGt                                                >  2:11954/1‑141 (MQ=255)
                                                                                           ataATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGa                                           <  2:265436/141‑1 (MQ=255)
                                                                                           ataATTGTAGCACTATTTATTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAAAGAATCGTTGGGAGAAAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGa                                           <  1:153545/141‑1 (MQ=255)
                                                                                              aTTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATTTTGCTGGAAAACCAAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                        <  1:292088/141‑1 (MQ=255)
                                                                                              aTTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAAtt                                        <  1:231305/141‑1 (MQ=255)
                                                                                                  tAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaaa                                    >  1:46252/1‑141 (MQ=255)
                                                                                                   aGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaaaa                                   >  2:38031/1‑140 (MQ=255)
                                                                                                   aGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaaaa                                   >  2:124399/1‑140 (MQ=255)
                                                                                                    gCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgt                                  >  1:205248/1‑141 (MQ=255)
                                                                                                     cACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtg                                 >  1:95105/1‑141 (MQ=255)
                                                                                                       ttaattCTTATGCTGTTTAATTACTATACTAAAAAGAAAAAATATACATCCACTGGTGATTGTATTGAAAAATTCTTTTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTc                               <  2:286662/137‑1 (MQ=255)
                                                                                                       cTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTc                               >  2:293624/1‑141 (MQ=255)
                                                                                                        tATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATATAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCt                              <  2:169504/141‑1 (MQ=255)
                                                                                                          tttCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAg                            >  1:98711/1‑141 (MQ=255)
                                                                                                            tCTTATGCTGTTTAATTACTATACAACAACTAGCAAACCTACATCTAAAGGTTAAATTCATGAAAAATAGAATTAGAAAATATAGAAAACAAAAAGAAATTTATCAAGAAACTTTAAATAAAGAATTAAAAGTATCTAgtc                          >  2:163742/1‑139 (MQ=255)
                                                                                                             cTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCa                         >  1:113940/1‑141 (MQ=255)
                                                                                                              ttATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaa                        >  1:133971/1‑141 (MQ=255)
                                                                                                               tATGCTGTTAAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaaa                       <  2:243792/141‑1 (MQ=255)
                                                                                                                 tGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATGTAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAACAATTAAAAGTGTCTAGGCAAACt                     >  2:159996/1‑141 (MQ=255)
                                                                                                                  gCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTa                    <  1:97647/141‑1 (MQ=255)
                                                                                                                    tGTTTAATTACTATAATACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAAAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTATGTAAAGAATTAAAAGTGTCTAGGCAAACTAtt                  <  2:260432/141‑1 (MQ=255)
                                                                                                                           ttACTATACTACAAATAAAAAATATACATCTCAGGGTACAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCaa           >  2:163653/1‑141 (MQ=255)
                                                                                                                           ttACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTACAAAAATCCAGTCAAACTATTAATGCaa           >  2:117686/1‑141 (MQ=255)
                                                                                                                            tactatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAAt          <  2:167280/141‑1 (MQ=255)
                                                                                                                                  actacAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaaaa    >  2:23644/1‑141 (MQ=255)
                                                                                                                                    tacAAATAAAAAATATACATCTAAAGGGTAAAGTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACa  >  1:36981/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                      
TTGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACA  >  CP000731/25524‑25797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: