BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	96,912:1	+A	6.4%	intergenic (+1428/+51)	gloB → / ← USA300HOU_0095	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	96,912	1	.	A	6.4%	733.6 / 37.3	172	intergenic (+1428/+51)	gloB/USA300HOU_0095	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (87/74); new base A (5/6); total (92/80)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01

Only 100 of 173 total aligned reads displayed.

GTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCAA  >  CP000730/96772‑97049

  gttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑Ga                                                                                                                                         <  2:1260009/141‑1 (MQ=255)

  gttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑Ga                                                                                                                                         >  1:1110555/1‑141 (MQ=255)

   ttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑Gaa                                                                                                                                        >  2:914316/1‑141 (MQ=255)

    tgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑Gaaa                                                                                                                                       <  1:1304539/141‑1 (MQ=255)

     gtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑Gaaaa                                                                                                                                      >  2:1028329/1‑141 (MQ=255)

           atatGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTC‑AATTAGAAAatatct                                                                                                                                 >  2:366329/1‑137 (MQ=38)

              tGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTC‑AATTAGAAAatatcttac                                                                                                                              <  2:1398389/141‑8 (MQ=37)

              tGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTC‑AATTAGAAAatatcttac                                                                                                                              <  1:372560/141‑8 (MQ=37)

                  gTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCActgct                                                                                                                         >  1:1066982/1‑141 (MQ=255)

                   tGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCActgctg                                                                                                                        <  2:798319/141‑1 (MQ=255)

                      aTTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTC‑AATTAGAAAAT‑ATCTTACTGCTGttt                                                                                                                     <  1:590330/141‑1 (MQ=25)

                        ttttAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGttttt                                                                                                                   <  1:1072193/141‑1 (MQ=255)

                         tttAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGtttttt                                                                                                                  >  1:518240/1‑141 (MQ=255)

                           tAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTC‑AATTAGAAAatatcttact                                                                                                                             >  1:783126/1‑121 (MQ=37)

                           tAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTC‑AATTAGAAAatatcttact                                                                                                                             <  2:783126/129‑9 (MQ=37)

                            aGCTCTAAATAGTATTAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                               <  1:1378564/141‑1 (MQ=255)

                            aGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                               <  1:272788/141‑1 (MQ=255)

                            aGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                               <  1:1182415/141‑1 (MQ=255)

                            aGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                               >  1:875277/1‑141 (MQ=255)

                             gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                              <  2:1613878/141‑1 (MQ=255)

                             gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                              >  1:1613878/1‑141 (MQ=255)

                             gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                              <  2:922645/141‑1 (MQ=255)

                              ctctAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAgg                                                                                                             <  2:648623/141‑1 (MQ=255)

                                 tAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGttt                                                                                                          >  1:1320919/1‑141 (MQ=255)

                                  aaacaGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTta                                                                                                         <  2:230375/137‑1 (MQ=255)

                                  aaaTAGTATAAGATTGAAAAAGGTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAAGT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTta                                                                                                         <  1:1546516/141‑1 (MQ=255)

                                        tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCTTACAATTTGGATGATTATTAATATAGAAACTTCAGTAATAT‑TTTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                   >  1:328073/1‑141 (MQ=37)

                                        tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                   <  1:48/141‑1 (MQ=255)

                                        tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                   <  1:234899/141‑1 (MQ=255)

                                        tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                   >  1:1335852/1‑141 (MQ=255)

                                        tataAGATTGAAAAAGTTGTTACTGCTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTCACTGCCGTTTTTTAAAGGTTTATACATc                                                                                                   >  1:1148286/1‑141 (MQ=255)

                                          taAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAATCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCAt                                                                                                 <  2:803487/141‑1 (MQ=255)

                                          taAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCAt                                                                                                 >  2:1351985/1‑141 (MQ=255)

                                            aGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATcc                                                                                               >  2:1534053/1‑141 (MQ=255)

                                            aGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATcc                                                                                               >  1:902411/1‑141 (MQ=255)

                                             gATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCct                                                                                              <  1:390993/141‑1 (MQ=255)

                                               ttGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCctct                                                                                            >  2:862269/1‑141 (MQ=255)

                                                tGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTa                                                                                           <  1:1646748/141‑1 (MQ=255)

                                                   aaaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAat                                                                                        >  1:47622/1‑141 (MQ=255)

                                                   aaaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAat                                                                                        >  1:1009765/1‑141 (MQ=255)

                                                    aaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAatt                                                                                       >  2:1375284/1‑141 (MQ=255)

                                                       gttgttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAattatt                                                                                    >  2:414878/1‑141 (MQ=255)

                                                           ttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaa                                                                                >  2:330675/1‑141 (MQ=255)

                                                           ttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaa                                                                                <  1:1138351/141‑1 (MQ=255)

                                                            tACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaaa                                                                               <  1:1422583/141‑1 (MQ=255)

                                                             aCTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTtaaaa                                                                              >  1:557850/1‑141 (MQ=255)

                                                              cTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaat                                                                             >  2:532895/1‑141 (MQ=255)

                                                               tGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaata                                                                            >  2:1169780/1‑141 (MQ=255)

                                                               tGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaata                                                                            >  2:1576231/1‑141 (MQ=255)

                                                               tGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaata                                                                            <  1:420656/141‑1 (MQ=255)

                                                                 ttttAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaataat                                                                          <  1:1463608/141‑1 (MQ=255)

                                                                  tttAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAAtaatt                                                                         <  2:1520023/141‑1 (MQ=255)

                                                                      aaTGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATg                                                                     >  1:1130384/1‑141 (MQ=255)

                                                                        tGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGt                                                                   >  2:966962/1‑141 (MQ=255)

                                                                          aTCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAACAATAATTAATGGTAt                                                                 >  2:1422887/1‑141 (MQ=255)

                                                                            cACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTAttt                                                               >  2:577693/1‑141 (MQ=255)

                                                                              cGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGa                                                             >  1:468674/1‑141 (MQ=255)

                                                                                aTGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGc                                                           >  1:1338643/1‑141 (MQ=255)

                                                                                  gAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCAc                                                         >  2:912739/1‑141 (MQ=255)

                                                                                   aaGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCActg                                                                                                                           <  1:292040/74‑1 (MQ=255)

                                                                                    aGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTATTTTTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGt                                                       >  2:1085231/1‑141 (MQ=255)

                                                                                       cATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTa                                                    <  2:1335852/141‑1 (MQ=255)

                                                                                          tCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCg                                                 <  2:746332/141‑1 (MQ=255)

                                                                                             aTAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACt                                              >  2:680293/1‑141 (MQ=255)

                                                                                                 gcaTGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAt                                          <  2:61155/139‑1 (MQ=255)

                                                                                                 gAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAt                                          >  1:121811/1‑141 (MQ=255)

                                                                                                  aaTGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATAGTATTTGAGCACGTTTATCTACTTTagt                                         >  2:1403040/1‑139 (MQ=255)

                                                                                                     gATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgact                                      >  2:148096/1‑141 (MQ=255)

                                                                                                      aTTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAACATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgactg                                     >  2:1583433/1‑141 (MQ=255)

                                                                                                       ttATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgactga                                    >  1:1428907/1‑141 (MQ=255)

                                                                                                          tGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                                 >  2:272498/1‑141 (MQ=255)

                                                                                                          tGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                                 >  2:147178/1‑141 (MQ=255)

                                                                                                           gAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAtt                                <  1:1631022/141‑1 (MQ=255)

                                                                                                            aaaaTAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTa                               <  1:150798/141‑1 (MQ=255)

                                                                                                            aaaaTAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTa                               <  1:541558/141‑1 (MQ=255)

                                                                                                             aaaTAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTAc                              <  2:252395/141‑1 (MQ=255)

                                                                                                                tAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTGCCTGCGGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCaa                           >  1:380362/1‑141 (MQ=255)

                                                                                                                  gAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAtt                         <  1:744318/141‑1 (MQ=255)

                                                                                                                   aaaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAttt                        >  1:638498/1‑141 (MQ=255)

                                                                                                                   aaaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAttt                        >  1:1174946/1‑141 (MQ=255)

                                                                                                                    aaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc                       >  2:1480104/1‑141 (MQ=255)

                                                                                                                    aaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc                       >  2:595716/1‑141 (MQ=255)

                                                                                                                    aaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc                       >  1:694785/1‑141 (MQ=255)

                                                                                                                     aCAGCAGTGAGATGATTTTC‑AATTAGAAAAT‑ATCTTACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTcc                      >  1:771958/1‑141 (MQ=37)

                                                                                                                     aCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTcc                      <  2:104274/141‑1 (MQ=255)

                                                                                                                     aCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTAGTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTcc                      <  2:428889/141‑1 (MQ=255)

                                                                                                                      cagcagTGAGATGATTTTC‑AATTAGAAAAT‑ATCTTACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCa                     >  1:1204955/1‑141 (MQ=37)

                                                                                                                       agcagTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCAt                    <  2:1521136/141‑1 (MQ=255)

                                                                                                                          agTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTCCCAATTTCCATTTc                 >  2:18170/1‑141 (MQ=255)

                                                                                                                              aGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGc             >  2:1305621/1‑141 (MQ=255)

                                                                                                                               gAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGcc            <  2:1133017/141‑1 (MQ=255)

                                                                                                                                 t‑atTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAg          <  1:1482579/141‑1 (MQ=255)

                                                                                                                                      ttCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGatat      <  2:466898/141‑1 (MQ=255)

                                                                                                                                      ttCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGatat      <  1:1362581/141‑1 (MQ=255)

                                                                                                                                       tCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATAtt     >  1:731975/1‑141 (MQ=255)

                                                                                                                                         tAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCa   <  1:1297080/141‑1 (MQ=255)

                                                                                                                                         tAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCa   <  1:147178/141‑1 (MQ=255)

                                                                                                                                          aaTT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCaa  <  2:894237/141‑1 (MQ=255)

                                                                                                                                          aaTT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCaa  <  1:363594/141‑1 (MQ=255)

                                                                                                                                          aaTT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCaa  >  2:20616/1‑141 (MQ=255)

GTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCAA  >  CP000730/96772‑97049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`