Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,448,493 C→T 8.2% intergenic (‑252/+25) hipO ← / ← hutI M20D subfamily peptidase/imidazolonepropionase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,448,4930CT 8.2% 481.5 / 23.9 159intergenic (‑252/+25)hipO/hutIM20D subfamily peptidase/imidazolonepropionase
Reads supporting (aligned to +/- strand):  ref base C (80/65);  new base T (8/5);  total (88/71)
Fisher's exact test for biased strand distribution p-value = 7.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01

Only 100 of 161 total aligned reads displayed.

TGATAGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGCATCCCAA  >  CP000730/2448354‑2448632
                                                                                                                                           |                                                                                                                                           
tGATAGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCa                                                                                                                                            >  1:239714/1‑141 (MQ=255)
tGATAGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTCTAAACCa                                                                                                                                            >  1:130579/1‑141 (MQ=255)
    aGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGc                                                                                                                                        >  1:897321/1‑141 (MQ=255)
     gACAGTTNTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGAATCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAACACGCTCTAAGCATTGGTTTAAACTAATgtt                                                                                                                                       <  2:1247920/141‑3 (MQ=255)
     gACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATGCAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCt                                                                                                                                       >  1:490781/1‑141 (MQ=255)
     gACAGTTGTTTAATAAGATCGTAAGACTGTTGTCAAACTATTCTAACTTTTATAAATGAGCCTCTATACAAAAACGGGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAACTCTAAACATTAGTGTAAACCAATGCt                                                                                                                                       >  2:414880/1‑141 (MQ=255)
      aCAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTACACCAATGCtt                                                                                                                                      <  2:1584192/141‑1 (MQ=255)
          ttgttTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTagag                                                                                                                                  <  1:182151/141‑1 (MQ=255)
             tttAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCtt                                                                                                                               <  2:64802/141‑1 (MQ=255)
              ttAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCttt                                                                                                                              >  1:191175/1‑141 (MQ=255)
               taataaTATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAACACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTc                                                                                                                             >  2:1059921/1‑141 (MQ=255)
                aataatATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCt                                                                                                                            <  2:1067762/141‑1 (MQ=255)
                aataatATCGTAACACCGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCt                                                                                                                            <  2:44741/141‑1 (MQ=255)
                 ataataTNGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTa                                                                                                                           <  2:1247257/141‑1 (MQ=255)
                 ataataTCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTa                                                                                                                           <  2:357796/141‑1 (MQ=255)
                                 gttgtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTGAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTa                                                                                                           <  1:877556/141‑1 (MQ=255)
                                 gttgtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTa                                                                                                           >  1:1366123/1‑141 (MQ=255)
                                  ttgtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACGACAAAAAGCTCTAAACATTAGGTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTaa                                                                                                          >  1:156140/1‑141 (MQ=255)
                                   tgtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTaaa                                                                                                         <  1:775684/141‑1 (MQ=255)
                                   tgtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTaaa                                                                                                         <  1:635989/141‑1 (MQ=255)
                                    gtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTaaaa                                                                                                        <  2:1084860/141‑1 (MQ=255)
                                       aaaCTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGAt                                                                                                     <  2:681286/141‑1 (MQ=255)
                                       aaaCTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGAt                                                                                                     <  2:616474/141‑1 (MQ=255)
                                       aaaCTATTCTAACTTTTATAATTGAGACTCGATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGAt                                                                                                     <  1:1188545/141‑1 (MQ=255)
                                         aCTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATAc                                                                                                   >  2:899100/1‑141 (MQ=255)
                                         aCTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATAc                                                                                                   >  2:16817/1‑141 (MQ=255)
                                         aCTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATAc                                                                                                   <  2:1014560/141‑1 (MQ=255)
                                           tATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACtg                                                                                                 <  2:168719/141‑1 (MQ=255)
                                           tATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACtg                                                                                                 <  1:309449/141‑1 (MQ=255)
                                            aTTCTAACCTTTATAATTGAGCCTCTATACAAAAACGTGTTCTAGCATATACTTGTTATTACAAACAACTAACAGCCCCTAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACtgt                                                                                                <  1:1402763/141‑1 (MQ=255)
                                             ttCTAACTTTTATAATTGAGACTCTATACAAAAACGTTTGCTCTAATAGACGTGGTTTTACATACCACAAAAAGCTCAAAACATCAGTTTAAACCAATTCTTAGAGCTTACAAATTACAGTATGCTTTAAAAGNTACtcga                                                                                               >  2:1242264/1‑138 (MQ=255)
                                             ttCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACtgtg                                                                                               >  2:764569/1‑141 (MQ=255)
                                               cTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGtt                                                                                             <  1:586761/141‑1 (MQ=255)
                                                tAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTa                                                                                            >  1:1488571/1‑141 (MQ=255)
                                                    ttttATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTa                                                                                        >  1:1338168/1‑141 (MQ=255)
                                                      ttATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTCCAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACg                                                                                      >  2:162643/1‑141 (MQ=255)
                                                      ttATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACg                                                                                      >  2:1220486/1‑141 (MQ=255)
                                                      ttATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACg                                                                                      <  2:467829/141‑1 (MQ=255)
                                                      gtataATTGAGACTCTATACAAAAACGTTTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTAca                                                                                      >  2:1479406/2‑140 (MQ=255)
                                                       tataATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTCCGTTATACTGTGTTATCTACGa                                                                                     >  1:851102/1‑141 (MQ=255)
                                                       tataATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGa                                                                                     >  1:379140/1‑141 (MQ=255)
                                                       tataATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGa                                                                                     >  1:404804/1‑141 (MQ=255)
                                                        ataATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGAt                                                                                    <  1:286534/141‑1 (MQ=255)
                                                         taATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATg                                                                                   >  1:1058943/1‑141 (MQ=255)
                                                         taATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATg                                                                                   >  1:910516/1‑141 (MQ=255)
                                                           aTTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGAc                                                                                 >  1:1480182/1‑141 (MQ=255)
                                                             taagaCTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCt                                                                               <  1:1539938/139‑1 (MQ=255)
                                                             tGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCt                                                                               >  2:1297967/1‑141 (MQ=255)
                                                              ttttctctATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTTAATCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCtt                                                                              >  2:847575/5‑141 (MQ=255)
                                                                gaCTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTAc                                                                            <  2:519828/141‑1 (MQ=255)
                                                                 aCTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTAcc                                                                           >  1:16926/1‑141 (MQ=255)
                                                                       taCAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTc                                                                                                                             <  1:1631245/85‑1 (MQ=255)
                                                                       taCAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCttt                                                                     >  1:113543/1‑141 (MQ=255)
                                                                       taCAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCttt                                                                     >  1:562254/1‑141 (MQ=255)
                                                                          aaaaaCGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTtaat                                                                  <  2:1492131/141‑1 (MQ=255)
                                                                            aaaCGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTtaataa                                                                >  2:740694/1‑141 (MQ=255)
                                                                             aaCGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAAc                                                               <  1:1340039/141‑1 (MQ=255)
                                                                              aCGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTCATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACt                                                              >  1:1606507/1‑141 (MQ=255)
                                                                                gtgtTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTTTTTTTTTTTTTTTCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACttt                                                            >  2:240781/1‑141 (MQ=255)
                                                                                gtgtTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATGATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACttt                                                            >  1:1570032/1‑141 (MQ=255)
                                                                                  gtTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACttttt                                                          <  1:509009/141‑1 (MQ=255)
                                                                                   ttCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTc                                                         >  2:953572/1‑141 (MQ=255)
                                                                                         aaTATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACATTCCCGTCTTTAATAACTTTTTCTGCGtg                                                   >  2:1167589/1‑141 (MQ=255)
                                                                                             taCTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGtgattg                                               <  1:643440/141‑1 (MQ=255)
                                                                                               cTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGtgattgat                                             <  2:915671/141‑1 (MQ=255)
                                                                                                ttGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTGTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATa                                            >  2:100329/1‑141 (MQ=255)
                                                                                                  gTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATAcc                                          <  2:790219/141‑1 (MQ=255)
                                                                                                        aCAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATg                                    >  1:110662/1‑141 (MQ=255)
                                                                                                        aCAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATg                                    <  2:1338168/141‑1 (MQ=255)
                                                                                                        aCAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATg                                    >  1:1212872/1‑141 (MQ=255)
                                                                                                             ccACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATg                               <  2:376490/141‑1 (MQ=255)
                                                                                                               acaAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGata                                 >  1:1287259/1‑137 (MQ=255)
                                                                                                               acaAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGata                                 <  2:1287259/137‑1 (MQ=255)
                                                                                                               acaAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGa                             >  1:28196/1‑141 (MQ=255)
                                                                                                                tagaaaGCTCTAAGCATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTtaa                                                                   <  2:1630402/99‑1 (MQ=25)
                                                                                                                tagaaaGCTCTAAGCATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTtaa                                                                   >  1:1630402/4‑102 (MQ=25)
                                                                                                                   aaaGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATGTGGAatat                         >  1:77257/1‑141 (MQ=255)
                                                                                                                     aGCTCTAAGCATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATACGCAatatat                       >  2:503175/1‑141 (MQ=38)
                                                                                                                     aGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAatatat                       >  1:694550/1‑141 (MQ=255)
                                                                                                                       ctctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  1:815975/141‑1 (MQ=255)
                                                                                                                       ctctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  1:1273052/141‑1 (MQ=255)
                                                                                                                       ctctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  1:16817/141‑1 (MQ=255)
                                                                                                                       ctctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  1:432154/141‑1 (MQ=255)
                                                                                                                       ctctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  1:1174207/141‑1 (MQ=255)
                                                                                                                       ctctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  2:704560/141‑1 (MQ=255)
                                                                                                                       ctctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTAATTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  2:786786/141‑1 (MQ=255)
                                                                                                                       ctctAAACATTAGTTGAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  1:1374896/141‑1 (MQ=255)
                                                                                                                        tctAAGCATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCa                    >  1:749157/1‑141 (MQ=39)
                                                                                                                        tctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCa                    <  1:419937/141‑1 (MQ=255)
                                                                                                                          taagcATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATg                  >  1:1024766/5‑141 (MQ=255)
                                                                                                                            agcATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATg                                    >  1:845786/3‑121 (MQ=39)
                                                                                                                            agcATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATg                                    <  2:845786/119‑1 (MQ=39)
                                                                                                                              cATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGAttt              >  1:902440/1‑141 (MQ=255)
                                                                                                                                ttggTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTgg            >  1:627966/4‑141 (MQ=255)
                                                                                                                                 tAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGt           >  2:1378267/1‑141 (MQ=255)
                                                                                                                                     ttAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCa                    <  1:1447761/128‑1 (MQ=255)
                                                                                                                                     ttAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCa                    >  2:1447761/1‑128 (MQ=255)
                                                                                                                                         aCCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGCATCCCa   >  1:869574/1‑141 (MQ=255)
                                                                                                                                          ccAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGCATCCCaa  <  2:865487/141‑1 (MQ=255)
                                                                                                                                          ccAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGCATCCCaa  <  1:1431154/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TGATAGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGCATCCCAA  >  CP000730/2448354‑2448632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: