Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,572 C→T 8.1% D214D (GAC→GAT USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5720CT 8.1% 444.2 / 15.6 142D214D (GAC→GATUSA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (68/62);  new base T (6/6);  total (74/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

GCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCTACATCT  >  CP000730/111434‑111712
                                                                                                                                          |                                                                                                                                              
gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                                                                              <  2:1143904/141‑1 (MQ=255)
gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                                                                                              <  1:109647/141‑1 (MQ=255)
 cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                                             <  1:565492/141‑1 (MQ=255)
 cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                                             <  1:235723/141‑1 (MQ=255)
 cAATATAGCAATTTTAAAGATTTAATGAATAATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCGCAAGATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                                                                                             >  2:570338/1‑141 (MQ=255)
   ttttaGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAcc                                                                                                                                           >  2:1341750/4‑141 (MQ=14)
    tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccg                                                                                                                                          <  2:387292/141‑2 (MQ=14)
    tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACca                                                                                                                                          <  2:1626902/141‑1 (MQ=255)
    tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACca                                                                                                                                          <  2:1188643/141‑1 (MQ=255)
      taGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTCACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgac                                                                                                                                        <  1:845055/141‑1 (MQ=11)
      taGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgac                                                                                                                                        <  1:1317223/141‑4 (MQ=14)
       aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacg                                                                                                                                       <  2:46789/141‑5 (MQ=11)
        gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                                                                                                                                      >  2:417367/1‑139 (MQ=11)
        gCAATTTTAAAGATTTAATGAACTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                                                                                                                                      >  2:1408045/1‑139 (MQ=11)
         aaaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacgag                                                                                                                                     <  1:626315/140‑7 (MQ=11)
          aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgacgagt                                                                                                                                    <  2:662059/141‑8 (MQ=11)
          aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAAt                                                                                                                                    >  2:1607721/1‑141 (MQ=255)
              ttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAg                                                                                                                                <  1:367463/141‑1 (MQ=255)
               tAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaatg                                                                                                                               >  1:533115/1‑139 (MQ=255)
               tAAAGATGTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgc                                                                                                                               <  2:542343/141‑1 (MQ=255)
                 aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                                                                                             <  2:1056107/141‑1 (MQ=255)
                 aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATAACAATTAACTAATGATGATTATAATGTACAACAATTAGGTAACAGATATGACAGACCAACAAATAAAgcgc                                                                                                                             >  1:59733/1‑141 (MQ=18)
                   gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCg                                                                                                                           <  1:1373747/141‑1 (MQ=255)
                    aTTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                                                                                          <  1:1043851/141‑1 (MQ=255)
                     tgtAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCGATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                                                                                         <  2:879879/139‑1 (MQ=255)
                      ttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAg                                                                                                                        >  1:621049/1‑141 (MQ=255)
                        aaTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCt                                                                                                                      <  1:1190366/141‑1 (MQ=255)
                          tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGATATTCAGCTCAATATCAATTAACTAATGATGGTTATAATGTAAAACAATTACGTACAAGATATGACATGCCAACAAATAAAGCGCCGAAGCtgt                                                                                                                    >  2:923376/1‑141 (MQ=255)
                            aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                                                                                                  <  2:949821/141‑1 (MQ=255)
                             aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttgt                                                                                                                 >  2:402585/1‑141 (MQ=255)
                               tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGttgttg                                                                                                               <  2:1217690/141‑1 (MQ=255)
                                    aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                                                                                          >  2:1626588/1‑141 (MQ=255)
                                     gATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGt                                                                                                         >  2:1088727/1‑141 (MQ=255)
                                       tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                                                                                       >  1:1532639/1‑141 (MQ=255)
                                       tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                                                                                       >  1:172168/1‑141 (MQ=255)
                                           gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                                   <  2:308767/141‑1 (MQ=255)
                                                  cATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaa                                                                                            <  1:1127121/141‑1 (MQ=255)
                                                   atatAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaaa                                                                                           >  1:1049823/1‑141 (MQ=255)
                                                    tataATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaaaa                                                                                          <  1:850285/141‑1 (MQ=255)
                                                      taATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAgg                                                                                        <  1:1465530/141‑1 (MQ=255)
                                                       aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                                       >  1:1516486/1‑141 (MQ=255)
                                                       aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                                       <  1:1067589/141‑1 (MQ=255)
                                                        tttatgagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                                      <  2:1531214/136‑1 (MQ=255)
                                                        aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                                      >  1:1126133/1‑141 (MQ=255)
                                                        aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                                                      <  2:443449/141‑1 (MQ=255)
                                                         tCCAGAGGTGCCGAGTTATTCAGCTCGATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                                     <  2:1311180/141‑1 (MQ=255)
                                                         tCCAGAGGTGCCGAGTTATTCAGCTCCATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                                     <  2:1311313/141‑1 (MQ=255)
                                                         tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                                     <  1:1064019/141‑1 (MQ=255)
                                                         tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                                     >  1:1391578/1‑141 (MQ=255)
                                                         tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                                     <  2:817225/141‑1 (MQ=255)
                                                         tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                                                     >  2:658745/1‑141 (MQ=255)
                                                          ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                                                    <  2:159259/141‑1 (MQ=255)
                                                           cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat                                                                                   >  1:756479/1‑141 (MQ=255)
                                                            agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatc                                                                                  <  1:336741/141‑1 (MQ=255)
                                                            agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatc                                                                                  >  1:1559669/1‑141 (MQ=255)
                                                                      gatctttCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAAAAATAAGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAATTGGTTCATCAgaaaaaaaa                                                                        <  2:759853/136‑9 (MQ=17)
                                                                      aGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGatat                                                                        <  2:15175/141‑1 (MQ=255)
                                                                         tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATTACATACCAACAAATAAAGTGCCGAAGCTGTTGTTGAAATGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑aat                                                                   >  2:224198/1‑140 (MQ=255)
                                                                         tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGGAAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                                                                                 >  1:45388/1‑81 (MQ=255)
                                                                                     tatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATCTGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGagatc                                                       >  1:759853/1‑137 (MQ=255)
                                                                                       tCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTAc                                                     <  1:981308/141‑1 (MQ=255)
                                                                                         aaTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACtt                                                   >  2:1322750/1‑141 (MQ=255)
                                                                                             aaCTAATGATTATGATAATTTATAAGAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAATCTGTTGTTGTAAGGTACAGGTTATTTAATAGTTTTATGAGTTTGATAT‑‑AAAAAAATTGAATGTACtttttt                                               <  2:984836/141‑1 (MQ=17)
                                                                                              aCTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTa                                              >  2:1329233/1‑141 (MQ=255)
                                                                                              aCTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTa                                              >  2:87231/1‑141 (MQ=255)
                                                                                                tAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTaga                                            >  1:832825/1‑141 (MQ=255)
                                                                                                   tgatgaTTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGaa                                         >  1:397108/1‑141 (MQ=255)
                                                                                                    gatgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAAt                                        <  1:263673/141‑1 (MQ=255)
                                                                                                     atgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATa                                       >  1:1339736/1‑141 (MQ=255)
                                                                                                      tgatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGAGAAGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTAATTTTTTAGAGAATaa                                      >  2:908437/1‑141 (MQ=255)
                                                                                                       gatTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATaaa                                     >  1:1152889/1‑141 (MQ=255)
                                                                                                         ttATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGATATTaaata                                   >  2:978785/1‑139 (MQ=255)
                                                                                                         ttATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATaaaaa                                   >  1:1439382/1‑141 (MQ=255)
                                                                                                         ttATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTGTAGAGAATaaaaa                                   >  2:747388/1‑141 (MQ=255)
                                                                                                           ataATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATg                                 >  1:1038937/1‑141 (MQ=255)
                                                                                                            taATGTAAAACAATTCCGTAAACGATACGACCTACCGACCGATAAAGGGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCTTCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGa                                <  1:1153021/141‑1 (MQ=18)
                                                                                                             aaTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATg                                 >  1:382319/1‑141 (MQ=255)
                                                                                                               tGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaaa                             >  2:703104/1‑141 (MQ=255)
                                                                                                               tGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaaaa                             <  2:887189/141‑1 (MQ=255)
                                                                                                                  aaaaCAATTACGTAAAAGATATGCCATACCAACAAAAAAAGAGCCCAAGCAGTAGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAatat                          >  2:59127/1‑141 (MQ=18)
                                                                                                                  aaaaCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAatat                          >  1:311512/1‑141 (MQ=255)
                                                                                                                   aaaCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtt                         <  2:911774/141‑1 (MQ=255)
                                                                                                                     aaaaTTACGTAAAAGATATGACATAACAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttta                       <  2:1004885/139‑1 (MQ=255)
                                                                                                                      cAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                                                                                   <  1:1608582/66‑1 (MQ=255)
                                                                                                                       aaTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttact                     <  2:1054584/141‑1 (MQ=255)
                                                                                                                        aTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttactt                    >  2:1606738/1‑141 (MQ=255)
                                                                                                                         ttACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATAAGTTAAAAAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttacttt                   <  2:461642/141‑1 (MQ=255)
                                                                                                                           aCGTAAAAGGTATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttactttac                 >  1:455188/1‑141 (MQ=255)
                                                                                                                           aCGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttactttac                 <  2:849764/141‑1 (MQ=255)
                                                                                                                           aCGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttactttac                 <  2:181131/141‑1 (MQ=255)
                                                                                                                           aCGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtttactttac                 >  2:536276/1‑141 (MQ=255)
                                                                                                                            cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATttactttact                >  2:135300/1‑141 (MQ=255)
                                                                                                                            cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATttactttact                >  2:547572/1‑141 (MQ=255)
                                                                                                                             gTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTg               >  1:688084/1‑141 (MQ=255)
                                                                                                                                    atatGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCt        <  2:698613/141‑1 (MQ=255)
                                                                                                                                    atatGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAAAAAAAAAA‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCt        <  2:698498/141‑1 (MQ=25)
                                                                                                                                        gACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCTACAt    >  1:1310466/1‑141 (MQ=255)
                                                                                                                                        gACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCTACAt    >  2:115033/1‑141 (MQ=255)
                                                                                                                                         aCATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCTACATc   >  1:1305139/1‑141 (MQ=255)
                                                                                                                                          cATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCTACATCt  >  1:683306/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                              
GCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATAT‑‑AAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTACTTTACTGATAGTCTACATCT  >  CP000730/111434‑111712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: