Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 372,114 C→A 6.0% intergenic (‑174/‑196) USA300HOU_0339 ← / → lip1 hypothetical membrane protein/triacylglycerol lipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730372,1140CA 6.0% 481.1 / 11.5 153intergenic (‑174/‑196)USA300HOU_0339/lip1hypothetical membrane protein/triacylglycerol lipase
Reads supporting (aligned to +/- strand):  ref base C (79/63);  new base A (8/2);  total (87/66)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 163 total aligned reads displayed.

TTAGAAAAATCTGAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGTTATATTGAAGG  >  CP000730/371974‑372253
                                                                                                                                            |                                                                                                                                           
ttAGAAAAATCTGAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAAcacc                                                                                                                                             >  1:1136836/1‑141 (MQ=255)
 tAGAAAAATCTGAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAAcacca                                                                                                                                            <  1:1445705/141‑1 (MQ=255)
 tAGAAAAATCTGAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAAcacca                                                                                                                                            <  1:1065178/141‑1 (MQ=255)
  aGAAAAATCTGAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAAcaccac                                                                                                                                           >  2:1639399/1‑141 (MQ=255)
   gAAAAATCTGAAAACTNACTATAATTGTANAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACcaca                                                                                                                                          >  1:1186470/1‑141 (MQ=255)
      aaaTCTGAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACt                                                                                                                                       <  1:1063/141‑1 (MQ=255)
        aTCTGAATACTTACTATAATTGTATAGTTTGAAGTATTTTCATACGAATACAAATTAACTTATTATATATAGTTTGAAACTTTATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTCGATTCTACACCCCAACTaa                                                                                                                                     >  1:1020121/1‑141 (MQ=255)
        aTCTGAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTaa                                                                                                                                     >  2:1286623/1‑141 (MQ=255)
        aTCTGAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTaa                                                                                                                                     >  1:845388/1‑141 (MQ=255)
         tCTGAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTaaa                                                                                                                                    >  2:1273740/1‑141 (MQ=255)
            gAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAgt                                                                                                                                 <  1:1580770/141‑1 (MQ=255)
                 cTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTta                                                                                                                            <  1:399583/141‑1 (MQ=255)
                 cTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTta                                                                                                                            >  1:1189645/1‑141 (MQ=255)
                  ttACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTtat                                                                                                                           <  2:1095134/141‑1 (MQ=255)
                    aCTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATaa                                                                                                                         >  1:995029/1‑141 (MQ=255)
                       ataATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAatt                                                                                                                      >  2:234143/1‑141 (MQ=255)
                       ataATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAatt                                                                                                                      >  2:129953/1‑141 (MQ=255)
                          aTTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAattatt                                                                                                                   <  2:590927/141‑1 (MQ=255)
                           ttGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTAttt                                                                                                                  <  1:1638070/141‑1 (MQ=255)
                             gTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCTCAACTAAAAGTGTTTATAAATTATTTgg                                                                                                                >  2:196467/1‑141 (MQ=255)
                             gTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTgg                                                                                                                <  1:1228176/141‑1 (MQ=255)
                                 aGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAAt                                                                                                            >  2:1147984/1‑141 (MQ=255)
                                       aaTTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACat                                                                                                      <  2:464971/141‑1 (MQ=255)
                                       aaTTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACat                                                                                                      <  2:322025/141‑1 (MQ=255)
                                          tattTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATAtt                                                                                                   <  2:121527/141‑1 (MQ=255)
                                           attTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATAttt                                                                                                  <  2:1332499/141‑1 (MQ=255)
                                            ttttCATACCAATACAAGTTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTg                                                                                                 >  2:1554798/1‑141 (MQ=255)
                                            ttttCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTg                                                                                                 >  2:1072350/1‑141 (MQ=255)
                                             tttCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGt                                                                                                <  1:575506/141‑1 (MQ=255)
                                             tttCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGt                                                                                                <  2:107060/141‑1 (MQ=255)
                                              ttCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTa                                                                                               <  1:757668/141‑1 (MQ=255)
                                                cATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTaaa                                                                                             >  1:233388/1‑141 (MQ=255)
                                                 aaaCCAATACAAAAAAACTAATTATATATAGATTGAAAATATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATACAACACCACAAATAAAAGTGTTTATAAATTATTTGGAAATACAAATATTTGTAAAt                                                                                            >  2:1175004/3‑141 (MQ=255)
                                                  tACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATg                                                                                           >  1:1607819/1‑141 (MQ=255)
                                                       aTACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATGTGTAAATGATTAg                                                                                      >  2:434062/1‑141 (MQ=255)
                                                         aCAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTa                                                                                    <  1:1351407/141‑1 (MQ=255)
                                                          cAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTAt                                                                                   >  2:387215/1‑141 (MQ=255)
                                                           aaaTTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATc                                                                                  <  2:280167/141‑1 (MQ=255)
                                                            aaTTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCg                                                                                 >  1:900588/1‑141 (MQ=255)
                                                            aaTTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCg                                                                                 >  2:377216/1‑141 (MQ=255)
                                                              ttAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGAt                                                                               >  2:1027668/1‑141 (MQ=255)
                                                                 aCTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTa                                                                            >  1:1293331/1‑141 (MQ=255)
                                                                 aCTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTa                                                                            >  2:188998/1‑141 (MQ=255)
                                                                    aaTTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAata                                                                         >  1:21631/1‑141 (MQ=255)
                                                                    aaTTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAata                                                                         >  1:996222/1‑141 (MQ=255)
                                                                    aaTTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGCGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAata                                                                         >  2:1386779/1‑141 (MQ=255)
                                                                     aTTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAatat                                                                        >  1:1240383/1‑141 (MQ=255)
                                                                     aTTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAatat                                                                        >  1:94321/1‑141 (MQ=255)
                                                                           tataGATTGAAACTATCTTACTTAATAAAATATTTATCTTAAATGTTGTCGTGTTCATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATAAATAGTATCGAATAAAAATAGtatt                                                                  >  2:984953/1‑141 (MQ=255)
                                                                            ataGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGtatta                                                                 >  2:324087/1‑141 (MQ=255)
                                                                              aGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTattatt                                                               <  2:218645/141‑1 (MQ=255)
                                                                              aGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTattatt                                                               <  2:1086904/141‑1 (MQ=255)
                                                                              aGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTattatt                                                               >  1:1521255/1‑141 (MQ=255)
                                                                                  tGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAAt                                                           <  1:1273740/141‑1 (MQ=255)
                                                                                  tGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAAt                                                           >  1:874457/1‑141 (MQ=255)
                                                                                    aaaCTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAAttt                                                         <  1:34494/141‑1 (MQ=255)
                                                                                     aaCTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAAtttt                                                        >  1:1395215/1‑141 (MQ=255)
                                                                                      aCTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAAttttt                                                       >  2:1200913/1‑141 (MQ=255)
                                                                                       ttataTTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACTCTATAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTtta                                                      <  1:410982/140‑1 (MQ=255)
                                                                                       cTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTtta                                                      <  1:312854/141‑1 (MQ=255)
                                                                                       cTAGATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTtta                                                      <  2:623459/141‑1 (MQ=255)
                                                                                         atatTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTttatt                                                    <  2:171043/141‑1 (MQ=255)
                                                                                         atatTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTttatt                                                    >  1:1328375/1‑141 (MQ=255)
                                                                                              aCTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAACTACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAAttt                                               >  1:1349360/1‑141 (MQ=255)
                                                                                                     aaaaTATTTATCTTAAATGTTGTTGTGTTGATTCAACACAACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTc                                        >  1:380471/1‑141 (MQ=255)
                                                                                                      aaaTATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCt                                       <  2:80433/141‑1 (MQ=255)
                                                                                                        atatTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTa                                     >  2:932545/1‑141 (MQ=255)
                                                                                                         tatTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTaa                                    <  1:1413659/141‑1 (MQ=255)
                                                                                                          atTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAAt                                   >  1:630168/1‑141 (MQ=255)
                                                                                                           tttATCTTAAATGTTGTTGTGTTGATTCAACACCTCAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATc                                  <  1:196467/141‑1 (MQ=255)
                                                                                                             tATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCaa                                >  2:973668/1‑141 (MQ=255)
                                                                                                             tATCATAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCaa                                <  1:348371/141‑1 (MQ=255)
                                                                                                              aTCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCaaa                               >  1:1122031/1‑141 (MQ=255)
                                                                                                                cTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCaaaaa                             >  2:653407/1‑141 (MQ=255)
                                                                                                                  tAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAaat                           <  1:1027668/141‑1 (MQ=255)
                                                                                                                    ttaggtgtggggttgAATCAACACAACAACTAACAGTGTTTATAAATTATTGGGGAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGGAGTCGTATTCAAAAaataa                         >  2:1403130/12‑141 (MQ=255)
                                                                                                                      tgttgttgtGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAataata                       <  2:1262575/141‑1 (MQ=255)
                                                                                                                        ttgttgtGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAatata                     >  1:228015/1‑141 (MQ=255)
                                                                                                                         tgttgtgttgATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGAAAAAATAAAAAAATAatatat                    <  2:995029/141‑1 (MQ=255)
                                                                                                                         tgtggtgttgAATCAACACAACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAatatat                    >  2:883620/1‑141 (MQ=255)
                                                                                                                          gttgtgttgATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGaaa                                                                                                             >  1:1638302/1‑51 (MQ=255)
                                                                                                                          gttgtgttgATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGaaa                                                                                                             <  2:1638302/51‑1 (MQ=255)
                                                                                                                          gttgtgttgATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATg                   >  2:1579683/1‑141 (MQ=255)
                                                                                                                           ttgtgttgATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTaa                                                                                              >  2:235536/1‑65 (MQ=255)
                                                                                                                           ttgtgttgATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTaa                                                                                              <  1:235536/65‑1 (MQ=255)
                                                                                                                           ttgtgttgATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGt                  >  2:1114548/1‑141 (MQ=255)
                                                                                                                           ttgtgttgATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCATAATCAAAAAATAATATATGt                  <  2:553169/141‑1 (MQ=255)
                                                                                                                              aataaaTTCAACACCAAAACTAAAATTGTTTATAAATTATTTGAAAATACAAATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCAt               <  1:531295/136‑1 (MQ=255)
                                                                                                                                ttgATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGt             >  1:1063534/1‑141 (MQ=255)
                                                                                                                                 tgATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGtt            <  1:1147984/141‑1 (MQ=255)
                                                                                                                                 tgAATCAACACAACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGtt            >  2:1160126/1‑141 (MQ=255)
                                                                                                                                    atCAACACAACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGTtata         >  1:1585634/2‑141 (MQ=255)
                                                                                                                                      caacacaacaACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGTTATAtt       >  2:1532813/1‑141 (MQ=255)
                                                                                                                                      cAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGTTATAtt       >  2:412008/1‑141 (MQ=255)
                                                                                                                                       atcaacacaACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGTTATATTg      >  1:813996/6‑141 (MQ=255)
                                                                                                                                       aaCACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGtacaatag      >  2:732270/1‑134 (MQ=255)
                                                                                                                                       aaCACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGTTATATTg      <  1:528348/141‑1 (MQ=255)
                                                                                                                                       aaCACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGTTATATTg      >  1:638405/1‑141 (MQ=255)
                                                                                                                                       aaCACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAAAAAAAAAAAAAAAATAATATATGTCATGTTATATTg      <  2:608668/141‑1 (MQ=255)
                                                                                                                                         aaccacAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAAAAAAAAAAAAAAAATAATATATGTCATGTTATATTGaa    <  2:130278/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TTAGAAAAATCTGAAAACTTACTATAATTGTATAGTTTGAATTATTTTCATACCAATACAAATTAACTAATTATATATAGATTGAAACTATATTACTTAATAAAATATTTATCTTAAATGTTGTTGTGTTGATTCAACACCACAACTAAAAGTGTTTATAAATTATTTGGAAATACACATATTTGTAAATGATTAGTATCGATTTAATATCGTATTATTAAATTTTTATTAATTTTGTAGTCTTAATCAAAAAATAATATATGTCATGTTATATTGAAGG  >  CP000730/371974‑372253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: