BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	740,322	G→C	5.7%	intergenic (+549/+39)	pitA → / ← sle1	possible PiT family inorganic phosphate transporter/possible LysM domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	740,322	0	G	C	5.7%	352.5 / 11.9	107	intergenic (+549/+39)	pitA/sle1	possible PiT family inorganic phosphate transporter/possible LysM domain protein
Reads supporting (aligned to +/- strand): ref base G (50/50); new base C (2/4); total (52/55)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 110 total aligned reads displayed.

TAATCAAATAGGTAGGTTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCATTTCAGAAATCA  >  CP000730/740182‑740455

taatCAAATAGGTAGGTTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatg                                                                                                                                        >  2:698028/1‑141 (MQ=255)

  atCAAATAGGTAGGTTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgat                                                                                                                                      <  2:741672/141‑1 (MQ=255)

    cAAATAGGTAGGTTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGAtgt                                                                                                                                    >  1:1623234/1‑141 (MQ=255)

       aTAGGTAGGTTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa‑a                                                                                                                                <  2:727829/141‑1 (MQ=255)

       aTAGGTAGGTTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTAACATGATGTGa‑a                                                                                                                                >  1:727865/1‑141 (MQ=255)

         aggtaggtTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTTTTTTTTTTTGAAAATTTTTTTCAAAAAAAAAAAACTAGCAATCTAACATGATGTAA‑Att                                                                                                                              >  2:749351/1‑141 (MQ=255)

         aggtaggtTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑Att                                                                                                                              >  1:1496882/1‑141 (MQ=255)

          ggtaggtTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAGTCTCAAATGATGTGA‑ATTg                                                                                                                             >  1:1646182/1‑141 (MQ=255)

          cgtaggtTGTTAATGGTTACCTAACTACCATTCATACAAAATATATATATTTATGATAGGGAAGGGTGAGATAATTTGTTGTACCAAAATGAATATTTTTTTCAAAAATAAAAATCTAGCAAT‑TCACATCATGTGagattg                                                                                                                             <  2:723058/140‑6 (MQ=255)

           gtaggtTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGc                                                                                                                            >  1:679846/1‑141 (MQ=255)

              ggtTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTAACAAAATAAAAATTTAATTAAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAg                                                                                                                         <  2:1580261/141‑1 (MQ=255)

               gttgttAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGt                                                                                                                        <  2:166927/141‑1 (MQ=255)

                ttgttAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGta                                                                                                                       >  2:1372337/1‑141 (MQ=255)

                    tAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtatata                                                                                                                   >  1:1161051/1‑141 (MQ=255)

                     aaTTTTTACCTAACTACAATTCATAAAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTATAAAAAAAAAAAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTatatat                                                                                                                  <  2:107325/141‑1 (MQ=255)

                      aTTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATc                                                                                                                 <  1:4498/141‑1 (MQ=255)

                        ttttACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATGGTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAg                                                                                                               <  1:743518/141‑1 (MQ=255)

                         tttACCTAACTACCATTCATACAAAATATAAATATGTATGATATGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGt                                                                                                              >  1:1621049/1‑141 (MQ=255)

                         tttACCAAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGt                                                                                                              <  2:1142691/141‑1 (MQ=255)

                          ttACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTa                                                                                                             <  1:717876/141‑1 (MQ=255)

                              cTAACTACCATTCAGACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAat                                                                                                         <  1:1206264/141‑1 (MQ=255)

                                 aCTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAattta                                                                                                      <  1:806577/141‑1 (MQ=255)

                                 aCTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAattta                                                                                                      >  2:183017/1‑141 (MQ=255)

                                 aCTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGGACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAattta                                                                                                      >  1:157373/1‑141 (MQ=255)

                                   tACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttatt                                                                                                    <  2:327516/141‑1 (MQ=255)

                                      cATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaa                                                                                                 <  2:694851/141‑1 (MQ=255)

                                       aTTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaat                                                                                                >  1:1414832/1‑141 (MQ=255)

                                        ttCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaatt                                                                                               >  1:905917/1‑141 (MQ=255)

                                         tCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaatta                                                                                              >  2:17456/1‑141 (MQ=255)

                                          cATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaattaa                                                                                             <  2:324254/141‑1 (MQ=255)

                                            tACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATg                                                                                           >  2:693836/1‑141 (MQ=255)

                                             aCAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATgg                                                                                          >  2:267130/1‑141 (MQ=255)

                                             aCAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATCGCTAGTATATATCAGTACAATTTATTTAATTAATgg                                                                                          >  2:415285/1‑141 (MQ=255)

                                               aaaaTATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAAAAAAAAAAAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGAt                                                                                        <  2:266916/141‑1 (MQ=255)

                                                aaatataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATg                                                                                       >  2:398770/1‑141 (MQ=255)

                                                 aatataaatatGTATGATAGGGAATGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGa                                                                                      >  1:1252524/1‑141 (MQ=255)

                                                 aatataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTTATAAATAAATATATAGTTATTTTCCATGTTGTGA‑ATTGCTAGTATTTATCAGTACAATTTCTTTAATTAATGGATgg                                                                                      >  2:345762/1‑140 (MQ=255)

                                                    ataaatatGTGTGATAGCGAAGAGTGAGATAATGCAGTAAACGAATAAGAAAATTTTTCGGAAAAATAAAGAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATg                                                                                   <  1:151718/141‑1 (MQ=255)

                                                     taaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTTAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGc                                                                                  >  2:815599/1‑141 (MQ=255)

                                                     taaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGc                                                                                  >  1:1358749/1‑141 (MQ=255)

                                                      aaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTCTATATCAGTAAAATTTATTTAATTAATGGatgcatgcc                                                                                 >  1:1319443/1‑140 (MQ=255)

                                                      aaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCa                                                                                 >  1:10691/1‑141 (MQ=255)

                                                        atatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATa                                                                               >  2:652965/1‑141 (MQ=255)

                                                          atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGc                                                                             <  1:533999/141‑1 (MQ=255)

                                                          atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGc                                                                             >  1:212676/1‑141 (MQ=255)

                                                           tgtatgATAGGGACCGGTGTGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCt                                                                            <  1:509717/141‑1 (MQ=255)

                                                           tgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCt                                                                            <  2:1143280/141‑1 (MQ=255)

                                                            gtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTa                                                                           <  2:249143/141‑1 (MQ=255)

                                                              atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGa                                                                         >  1:223687/1‑141 (MQ=255)

                                                               tgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGaa                                                                        <  2:1265845/141‑1 (MQ=255)

                                                                  tAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACt                                                                     <  2:518233/141‑1 (MQ=255)

                                                                   aGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACtt                                                                    >  2:638926/1‑141 (MQ=255)

                                                                    gggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTc                                                                   <  2:1267469/141‑1 (MQ=255)

                                                                      gAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTg                                                                 <  1:325168/141‑1 (MQ=255)

                                                                       aaGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGa                                                                >  1:222841/1‑141 (MQ=255)

                                                                        aGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGaa                                                               <  2:1294244/141‑1 (MQ=255)

                                                                        aGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGaa                                                               <  1:523159/141‑1 (MQ=255)

                                                                            tGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTg                                                           <  1:183017/141‑1 (MQ=255)

                                                                             gagaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTgg                                                          <  2:28108/141‑1 (MQ=255)

                                                                             gagaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTgg                                                          <  1:266362/141‑1 (MQ=255)

                                                                              agaTAATTTTTTGTACGAAAATTAAAATTTGGTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGa                                                         <  2:827350/141‑1 (MQ=255)

                                                                              agaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGa                                                         <  2:679846/141‑1 (MQ=255)

                                                                              agaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGa                                                         >  1:833667/1‑141 (MQ=255)

                                                                              agaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGa                                                         >  1:833658/1‑141 (MQ=255)

                                                                               gaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGaa                                                        >  2:1192647/1‑141 (MQ=255)

                                                                                   atattttGAACCAAAATGAAAATTTTTTTCAAAAATAAAAAGTCAGCAATCTCACCTACCGGTA‑ATTGCTAGTATATATCAGCACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGt                                                    <  1:29608/138‑1 (MQ=255)

                                                                                   aTTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGt                                                    >  1:1371656/1‑141 (MQ=255)

                                                                                    ttttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTa                                                   >  1:1628992/1‑141 (MQ=255)

                                                                                     tttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTAc                                                  >  2:764248/1‑141 (MQ=255)

                                                                                       tttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACgg                                                <  2:26719/141‑1 (MQ=255)

                                                                                        ttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGt                                               >  1:1130817/1‑141 (MQ=255)

                                                                                         tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTa                                              >  1:678244/1‑141 (MQ=255)

                                                                                         tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTa                                              <  2:961561/141‑1 (MQ=255)

                                                                                         tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTa                                              <  1:1372337/141‑1 (MQ=255)

                                                                                            aCCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGtt                                           <  2:968757/141‑1 (MQ=255)

                                                                                            aCCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGtt                                           <  2:1298044/141‑1 (MQ=255)

                                                                                             ccAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGGGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGGATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTc                                          >  1:622951/1‑141 (MQ=255)

                                                                                                  aTGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATAtt                                     <  2:212676/141‑1 (MQ=255)

                                                                                                   tGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTg                                    <  2:1505780/141‑1 (MQ=255)

                                                                                                      aaaTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTAt                                 <  2:203302/141‑1 (MQ=255)

                                                                                                       aaTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATg                                >  1:928803/1‑141 (MQ=255)

                                                                                                        aTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATgg                               <  1:1117964/141‑1 (MQ=255)

                                                                                                         tttttttCAAAAATAAAAAACTAGCAATCTCACATGAGGTTA‑ATTGCTAGTTTATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGa                              >  2:1654508/1‑141 (MQ=255)

                                                                                                              ttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCata                         >  1:331093/1‑141 (MQ=255)

                                                                                                              ttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCata                         >  2:1147278/1‑141 (MQ=255)

                                                                                                                  aaaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATAtgtg                     <  2:451104/141‑1 (MQ=255)

                                                                                                                       aaaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAAtt                >  2:1522116/1‑141 (MQ=255)

                                                                                                                         aaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCa              >  2:557198/1‑141 (MQ=255)

                                                                                                                          aaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCAt             >  1:1392781/1‑141 (MQ=255)

                                                                                                                          aaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCAt             <  1:1192647/141‑1 (MQ=255)

                                                                                                                            aCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGt                                   >  2:1340627/1‑117 (MQ=255)

                                                                                                                            aCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGt                                   <  1:1340627/117‑1 (MQ=255)

                                                                                                                            aCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCAttt           >  2:216612/1‑141 (MQ=255)

                                                                                                                             cTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCATTTc          <  1:1274773/141‑1 (MQ=255)

                                                                                                                             cTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCATTTc          <  1:17456/141‑1 (MQ=255)

                                                                                                                              tAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCATTTCa         >  2:491205/1‑141 (MQ=255)

                                                                                                                                    tctcACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTAAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCATTTCAGAAATc   <  1:267130/141‑1 (MQ=255)

                                                                                                                                     ctcACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCATTTCAGAAATCa  <  1:539739/141‑1 (MQ=255)

                                                                                                                                      tcACATCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCt                                                                            <  2:867695/67‑1 (MQ=38)

                                                                                                                                      tcACATCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCt                                                                            >  1:867695/1‑67 (MQ=38)

TAATCAAATAGGTAGGTTGTTAATTTTTACCTAACTACCATTCATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATATTGTATGGACCATATGTGTAATTCATTTCAGAAATCA  >  CP000730/740182‑740455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`