Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 942,216 T→A 7.9% intergenic (‑187/+62) USA300HOU_0905 ← / ← mnhG2 hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730942,2160TA 7.9% 439.0 / 21.1 138intergenic (‑187/+62)USA300HOU_0905/mnhG2hypothetical protein/CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit G
Reads supporting (aligned to +/- strand):  ref base T (66/61);  new base A (7/4);  total (73/65)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 138 total aligned reads displayed.

TATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATA  >  CP000730/942079‑942355
                                                                                                                                          |                                                                                                                                           
tattatCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCATAAt                                                                                                                                          >  2:486325/1‑141 (MQ=255)
 attatCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAACAAGAGCTAACAATGTCACAAGAAAACAGCAGTAAAATCATTACTAAtt                                                                                                                                         >  2:1477019/1‑141 (MQ=255)
   gataataataCATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTg                                                                                                                                       >  1:1102867/5‑141 (MQ=255)
     tCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTTTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGa                                                                                                                                     >  1:1074441/1‑141 (MQ=255)
           taCATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatcatc                                                                                                                               >  2:1611857/1‑141 (MQ=255)
             cATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCtt                                                                                                                             >  2:11580/1‑141 (MQ=255)
              aTAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa                                                                                                                            >  1:74351/1‑141 (MQ=255)
              aTAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa                                                                                                                            >  2:359720/1‑141 (MQ=255)
                 aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActg                                                                                                                         >  2:1190374/1‑141 (MQ=255)
                 aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActg                                                                                                                         <  2:183094/141‑1 (MQ=255)
                 aCGACATGAAAGGCAAATAAATTAAAGTGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActg                                                                                                                         <  2:902570/141‑1 (MQ=255)
                  cGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATATGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAACTGAAAACAGCAGTAAGATGATTCCAAATTAGGAATGATTTTActgc                                                                                                                        <  2:153209/141‑1 (MQ=37)
                    aCATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActgctg                                                                                                                      >  2:732198/1‑141 (MQ=255)
                      aTGAAAGGCAATTAAATTAAAGATATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtt                                                                                                                    <  1:593830/141‑1 (MQ=255)
                       tGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCggttt                                                                                                                   >  2:1335635/1‑141 (MQ=255)
                        gAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTTACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtttg                                                                                                                  <  1:486325/141‑1 (MQ=255)
                        gAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtttg                                                                                                                  <  1:437904/141‑1 (MQ=255)
                        gAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtttg                                                                                                                  <  1:438324/141‑1 (MQ=255)
                            ggCAATTGAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGGAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTttgttgt                                                                                                              >  2:581982/1‑141 (MQ=255)
                             gCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTttgttgt                                                                                                              <  2:553887/141‑1 (MQ=255)
                               aattaaattaAAGAGATATATGTAGATAGGGCGAATCTGTAGTAAAAAAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGa                                                                                                           <  2:1268467/141‑1 (MQ=255)
                                 ttaaattaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGAtt                                                                                                         >  1:872921/1‑141 (MQ=255)
                                  taaattaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGAttt                                                                                                        >  2:1036357/1‑141 (MQ=255)
                                   aaattaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTta                                                                                                       >  1:1221340/1‑141 (MQ=255)
                                     attaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTtata                                                                                                     >  2:798873/1‑141 (MQ=255)
                                      ttaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTatat                                                                                                    >  1:24875/1‑141 (MQ=255)
                                       taaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATAtt                                                                                                   <  2:663884/141‑1 (MQ=255)
                                        aaaGATATATATGTAGATAGGGCGAATCAGAAGAAAAAAAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTc                                                                                                  <  2:63834/141‑1 (MQ=255)
                                          agagATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTATCAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCAt                                                                                                >  2:1002049/1‑141 (MQ=255)
                                           gagaTATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATg                                                                                               <  1:1456130/141‑1 (MQ=255)
                                              atatatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGAtt                                                                                            >  1:1208909/1‑141 (MQ=255)
                                              atatatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGAtt                                                                                            >  1:70091/1‑141 (MQ=255)
                                                 tatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTg                                                                                         <  1:920740/141‑1 (MQ=255)
                                                  atGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAGACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGt                                                                                        <  1:83482/141‑1 (MQ=255)
                                                      aGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATGATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTtata                                                                                    >  2:855535/1‑141 (MQ=255)
                                                      aGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTtata                                                                                    >  1:67736/1‑141 (MQ=255)
                                                       gATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTtatat                                                                                   >  2:1134142/1‑141 (MQ=255)
                                                           gggCGAATCTGTAGTCAAGCAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATc                                                                               <  1:1335790/141‑1 (MQ=255)
                                                           gggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATc                                                                               >  1:15497/1‑141 (MQ=255)
                                                            ggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCt                                                                              <  2:341537/141‑1 (MQ=255)
                                                             gCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa                                                                             >  2:872289/1‑141 (MQ=255)
                                                             gCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa                                                                             >  2:926134/1‑141 (MQ=255)
                                                             gCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa                                                                             <  2:1419065/141‑1 (MQ=255)
                                                              cGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACATTGTCAAGAGAAGACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTAc                                                                            <  1:1396943/141‑1 (MQ=255)
                                                                  tCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAtt                                                                        <  1:704480/141‑1 (MQ=255)
                                                                      tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCAt                                                                                                >  1:20867/1‑113 (MQ=255)
                                                                      tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCAt                                                                                                <  2:20867/113‑1 (MQ=255)
                                                                      tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt                                                                    >  1:1006456/1‑141 (MQ=255)
                                                                      tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt                                                                    <  2:618093/141‑1 (MQ=255)
                                                                      tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt                                                                    <  1:300787/141‑1 (MQ=255)
                                                                           aaaGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtt                                                               <  1:870647/141‑1 (MQ=255)
                                                                             aGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtttt                                                             >  2:1513748/1‑141 (MQ=255)
                                                                             aGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtttt                                                             <  1:798873/141‑1 (MQ=255)
                                                                             aGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtttt                                                             <  1:767857/141‑1 (MQ=255)
                                                                             aGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATATTACAGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtttt                                                             >  2:1513493/1‑141 (MQ=255)
                                                                               aaaaaaTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTaa                                                           >  1:1201559/1‑141 (MQ=255)
                                                                                aaaaaTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAg                                                          <  1:1499062/141‑1 (MQ=255)
                                                                                  aaaTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTc                                                        >  1:691992/1‑141 (MQ=255)
                                                                                    aTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCtt                                                      >  2:1419638/1‑141 (MQ=255)
                                                                                    aTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCtt                                                      >  2:119698/1‑141 (MQ=255)
                                                                                    aTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCtt                                                      <  1:971993/141‑1 (MQ=255)
                                                                                     tCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTc                                                     >  2:1404787/1‑141 (MQ=255)
                                                                                        ttGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGa                                                  <  2:1284380/141‑1 (MQ=255)
                                                                                            aaaaaGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAAt                                               <  2:603662/141‑1 (MQ=255)
                                                                                            aaaaaGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAAt                                               >  1:1263212/1‑141 (MQ=255)
                                                                                             aaaaGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAAtt                                              >  2:638136/1‑141 (MQ=255)
                                                                                                agagGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCa                                           <  2:966474/141‑1 (MQ=255)
                                                                                                  agGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATc                                         >  1:1517575/1‑141 (MQ=255)
                                                                                                       aCAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACtt                                    <  2:15497/141‑1 (MQ=255)
                                                                                                        cAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACttt                                   <  2:602497/141‑1 (MQ=255)
                                                                                                         aaTGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATGATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTa                                  <  1:748865/141‑1 (MQ=255)
                                                                                                         aaTGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTa                                  <  2:904614/141‑1 (MQ=255)
                                                                                                          aTGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAg                                 <  2:67736/141‑1 (MQ=255)
                                                                                                           tGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGt                                <  2:741152/141‑1 (MQ=255)
                                                                                                               aaaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCttt                            >  2:431075/1‑141 (MQ=255)
                                                                                                               aaaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCttt                            >  1:1067560/1‑141 (MQ=255)
                                                                                                               aaaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCttt                            >  1:254902/1‑141 (MQ=255)
                                                                                                                aaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCtttt                           <  2:217849/141‑1 (MQ=255)
                                                                                                                  aGAAAACAACAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTa                         >  2:1375111/1‑141 (MQ=255)
                                                                                                                     aaaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTc                               >  1:1405353/1‑132 (MQ=255)
                                                                                                                      aaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGtat                     >  2:111043/1‑141 (MQ=255)
                                                                                                                      aaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGtat                     <  2:870556/141‑1 (MQ=255)
                                                                                                                       aCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGtata                    >  2:227611/1‑141 (MQ=255)
                                                                                                                        cagcagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTATAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATaa                   <  2:1507731/141‑1 (MQ=255)
                                                                                                                         agcagTAAGATGATTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAg                  >  1:293452/1‑141 (MQ=37)
                                                                                                                         agcagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAg                  >  1:493550/1‑141 (MQ=255)
                                                                                                                         agcagTAAAATCATTCCTAATTTGAAAACATCTTCCTGCTGATTGTTGTTGATTTCTATACATGATTAAGTTATATAATCAAACATCTTGTGTTTAATAAGTCTTACGAAATATCAACGACTTTAGTCTTTTTAGTATAAg                  >  1:486588/1‑141 (MQ=255)
                                                                                                                          gcagTAAGATGATTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAgg                 >  1:1637923/1‑141 (MQ=37)
                                                                                                                           cagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGc                <  1:186608/141‑1 (MQ=255)
                                                                                                                           cagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGc                <  2:15737/141‑1 (MQ=255)
                                                                                                                           cagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGc                <  2:762438/141‑1 (MQ=255)
                                                                                                                            agTAAGATGATTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCg               >  1:348363/1‑141 (MQ=38)
                                                                                                                            agTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCg               <  1:1036357/141‑1 (MQ=255)
                                                                                                                             gTAAGATGATTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGt              >  2:489986/1‑141 (MQ=38)
                                                                                                                             gTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGt              <  2:931390/141‑1 (MQ=255)
                                                                                                                              tAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtt             <  2:690777/141‑1 (MQ=255)
                                                                                                                                agatgaTTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtttt           >  1:1043900/6‑141 (MQ=38)
                                                                                                                                agatgaTTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtttt           >  1:1250214/6‑141 (MQ=38)
                                                                                                                                  aTCATTCCTAATTTGGNATCATCTTACTGNTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTaa         >  1:1328384/1‑141 (MQ=255)
                                                                                                                                       tCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAAtatta    >  2:727256/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TATTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATA  >  CP000730/942079‑942355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: