BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,333,796	T→G	10.4%	V66G (GTT→GGT)	hflX →	GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,333,796	0	T	G	10.4%	343.9 / 3.4	114	V66G (GTT→GGT)	hflX	GTP‑binding protein
Reads supporting (aligned to +/- strand): ref base T (41/59); new base G (12/2); total (53/61)
Fisher's exact test for biased strand distribution p-value = 2.92e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

GTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAGAAC  >  CP000730/1333658‑1333934

gtgtACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTg                                                                                                                                          >  2:817092/1‑141 (MQ=255)

  gtACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggt                                                                                                                                        >  2:129388/1‑141 (MQ=255)

  gtACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggt                                                                                                                                        <  1:537613/141‑1 (MQ=255)

       tGCTCAAGATGATAAGCNATTTAATTGTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaagg                                                                                                                                   <  1:1120264/141‑1 (MQ=255)

        gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaaggt                                                                                                                                  <  2:897852/141‑1 (MQ=255)

        gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaaggt                                                                                                                                  >  1:1288182/1‑141 (MQ=255)

        gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaaggt                                                                                                                                  <  2:274148/141‑1 (MQ=255)

        gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaaggt                                                                                                                                  <  2:78714/141‑1 (MQ=255)

        gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaaggt                                                                                                                                  <  1:1159493/141‑1 (MQ=255)

           cAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTggtaaaggtaaa                                                                                                                               <  1:467545/141‑1 (MQ=255)

              gatgatAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAAtt                                                                                                                            >  2:593136/1‑141 (MQ=255)

               atgatAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTg                                                                                                                           <  2:1233920/141‑1 (MQ=255)

                tgatAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTga                                                                                                                          <  1:21614/141‑1 (MQ=255)

                  atAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTgaag                                                                                                                        <  1:1329083/141‑1 (MQ=255)

                   tAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTgaaga                                                                                                                       >  2:74140/1‑141 (MQ=255)

                   tAAGCAATTTAATTTTGAGTCTACAATGAAGGAAATATAATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTgaaga                                                                                                                       >  1:880875/1‑141 (MQ=255)

                     aGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGaaa                                                                                                                     >  1:1143319/1‑141 (MQ=255)

                     aGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGaaa                                                                                                                     >  1:1094822/1‑141 (MQ=255)

                     aGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGaaa                                                                                                                     >  1:1261573/1‑141 (MQ=255)

                      gCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAAt                                                                                                                    <  1:817092/141‑1 (MQ=255)

                          tttaatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCaa                                                                                                                <  1:529789/141‑1 (MQ=255)

                          tttaatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCaa                                                                                                                <  1:805073/141‑1 (MQ=255)

                          tttaattnTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAATTTGAAGAAATTCaa                                                                                                                <  2:1244233/141‑1 (MQ=255)

                           ttaatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAg                                                                                                               >  1:389559/1‑141 (MQ=255)

                           ttaatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAg                                                                                                               <  1:1453093/141‑1 (MQ=255)

                            taatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGc                                                                                                              <  2:1252430/141‑1 (MQ=255)

                            taatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGc                                                                                                              <  1:1517285/141‑1 (MQ=255)

                             aatttTGAGTTTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTTAAGTGTTGGGTCAAATTGCTAAAAACAGTGATCGTGTAGATCGGAAATATTATGTTGGTAAAGGTAAAATTGAAGATTTTCAGGCa                                                                                                             <  2:123470/141‑1 (MQ=255)

                             aatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCa                                                                                                             >  1:771022/1‑141 (MQ=255)

                             aatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTCGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCa                                                                                                             <  1:108942/141‑1 (MQ=255)

                             aatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACGTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCa                                                                                                             <  2:368976/141‑1 (MQ=255)

                               ttttGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCAtt                                                                                                           >  1:874443/1‑141 (MQ=255)

                                  tGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTACGGTGGTAAAGGTAAAAATGAAGAAATTCAAGCATTTAt                                                                                                        >  2:757402/1‑141 (MQ=255)

                                   gAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCGGAGATTGCGGTAGCAAAAGAAAAATAAAAGAAATTCAAGCATTTAtt                                                                                                       >  2:1498814/1‑141 (MQ=255)

                                   gAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTAtt                                                                                                       <  2:167121/141‑1 (MQ=255)

                                    aGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTg                                                                                                      <  1:1545863/141‑1 (MQ=255)

                                       cTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGt                                                                                                   <  1:109931/141‑1 (MQ=255)

                                         aCAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAGAGGTCATGGTAGCAAAGGAAAAAATGAAGAAATTCAAGCATTTATTGAGTTc                                                                                                 >  2:843949/1‑141 (MQ=255)

                                         aCAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTc                                                                                                 <  1:288280/141‑1 (MQ=255)

                                           aaTGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCaa                                                                                               <  2:1638028/141‑1 (MQ=255)

                                            aTGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCaaa                                                                                              <  1:1328261/141‑1 (MQ=255)

                                            aTGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCaaa                                                                                              <  1:673948/141‑1 (MQ=255)

                                              ggaggaATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCGGAGATTACCGTGGCAAAAGAAAAAATAAAGAAATTCAAGCATTTATTGAGTTCAAAGa                                                                                            >  2:1127744/1‑141 (MQ=255)

                                               gaggaATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATTGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGat                                                                                           >  2:1001597/1‑141 (MQ=255)

                                                aggaATTGGCAGCTTTATCAGAGGCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGAGGGCAAATGTGATGGTGGTAGAGGTAGAATGTAGGAAATTCAAGCATTTATTGGGTTCAAAGata                                                                                          <  2:1396306/141‑1 (MQ=255)

                                                 ggaATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGATATCACCGTAGCATTGATAAAATTAAAGAAATTCAAGCATTTATTGAGTTCAAAGatat                                                                                         >  2:1558075/1‑141 (MQ=255)

                                                    aTTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGa                                                                                      <  1:1392185/141‑1 (MQ=255)

                                                           tcTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCGGTGGTCGCCGTAGCATAAAAAAAAAAGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtc                                                                               >  2:1559486/1‑141 (MQ=255)

                                                                aTCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATcac                                                                          <  1:424923/141‑1 (MQ=255)

                                                                 tCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATcaca                                                                         <  1:1417596/141‑1 (MQ=255)

                                                                  cAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACaa                                                                        <  2:505151/141‑1 (MQ=255)

                                                                   agagACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACaaa                                                                       >  1:470060/1‑141 (MQ=255)

                                                                    gagaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTGAAGTTGAAGAAGTTCAAGCATTTATTGAGTTCAAAGATATGGATGTAGTCATCACAAat                                                                      >  1:67008/1‑141 (MQ=255)

                                                                     agaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATTGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatg                                                                     <  1:1001597/141‑1 (MQ=255)

                                                                     agaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatg                                                                     >  1:1285226/1‑141 (MQ=255)

                                                                      gaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGGTCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatga                                                                    <  1:1478952/141‑1 (MQ=255)

                                                                      gaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatga                                                                    >  1:1332166/1‑141 (MQ=255)

                                                                         ttGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAAtgatga                                                                 >  2:1417548/1‑141 (MQ=255)

                                                                          ttccAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGaa                                                                >  2:1366886/3‑141 (MQ=255)

                                                                             cAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTa                                                             >  1:848551/1‑141 (MQ=255)

                                                                              aacacttcaagtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCGGATATTACGGTAGCAAAGGAAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTaa                                                            >  2:1126924/11‑141 (MQ=255)

                                                                                cTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACg                                                          <  1:95342/141‑1 (MQ=255)

                                                                                       tgtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtcatc                                                                            <  2:203707/116‑1 (MQ=255)

                                                                                       tgtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtcatc                                                                            >  1:203707/1‑116 (MQ=255)

                                                                                       tgtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtcatc                                                                            >  1:202988/1‑116 (MQ=255)

                                                                                       tgtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtcatc                                                                            <  2:202988/116‑1 (MQ=255)

                                                                                       tgtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGcac                                                   <  2:746189/141‑1 (MQ=255)

                                                                                                 aaTTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCaa                                                                                                                <  2:43669/70‑1 (MQ=255)

                                                                                                 aaTTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCaa                                                                                                                >  1:43669/1‑70 (MQ=255)

                                                                                                     aCTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATGACAAATGATGAATTAACGACTGCACAATCCAAATCACTa                                     >  1:1224887/1‑141 (MQ=255)

                                                                                                     aCTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTa                                     >  1:85265/1‑141 (MQ=255)

                                                                                                       tCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTaaa                                   <  2:1052304/141‑1 (MQ=255)

                                                                                                        cAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAAt                                  <  1:757402/141‑1 (MQ=255)

                                                                                                           aaCAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGaa                               <  2:1404545/141‑1 (MQ=255)

                                                                                                            aCAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAg                              <  1:1207466/141‑1 (MQ=255)

                                                                                                                agaTCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCttt                          >  1:221117/1‑141 (MQ=255)

                                                                                                                agaTCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCttt                          >  1:1127110/1‑141 (MQ=255)

                                                                                                                agaTCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCttt                          >  1:450408/1‑141 (MQ=255)

                                                                                                                  aTCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAg                        <  1:1018456/141‑1 (MQ=255)

                                                                                                                     gtgtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGgtg                     >  1:778988/1‑141 (MQ=255)

                                                                                                                      tgtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGgtgt                    <  2:1143319/141‑1 (MQ=255)

                                                                                                                      tgtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGgtgt                    <  1:74140/141‑1 (MQ=255)

                                                                                                                      tgtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGgtgt                    <  2:1143071/141‑1 (MQ=255)

                                                                                                                       gtggagcGCAAATATTATGGTGGTAAAGGTAAAATTGAAGGAATTCGAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGTTGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTa                   <  2:658544/135‑1 (MQ=255)

                                                                                                                       gtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTa                   <  1:1384987/141‑1 (MQ=255)

                                                                                                                       gtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTa                   <  1:129388/141‑1 (MQ=255)

                                                                                                                       gtAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTa                   <  2:1444204/141‑1 (MQ=255)

                                                                                                                         aGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTaaa                 >  1:1022791/1‑141 (MQ=255)

                                                                                                                            tCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAat              >  1:1153411/1‑141 (MQ=255)

                                                                                                                            tCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAat              >  1:681551/1‑141 (MQ=255)

                                                                                                                              gCAAATATTATGTTGGTAAGGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAatta            <  2:427571/141‑1 (MQ=255)

                                                                                                                                 aaTATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTg         >  1:1181449/1‑141 (MQ=255)

                                                                                                                                   tattatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCaa                                            >  1:1301785/1‑104 (MQ=255)

                                                                                                                                   tattatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCaa                                            <  2:1301785/104‑1 (MQ=255)

                                                                                                                                    attatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATa      <  2:1118445/141‑1 (MQ=255)

                                                                                                                                     ttatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAg     <  2:457226/141‑1 (MQ=255)

                                                                                                                                     ttatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAg     <  1:672367/141‑1 (MQ=255)

                                                                                                                                      tatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAGa    >  2:1294172/1‑141 (MQ=255)

                                                                                                                                      tatGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAGa    >  1:359097/1‑141 (MQ=255)

                                                                                                                                        tGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAGAAc  >  2:1425958/1‑141 (MQ=255)

GTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATATTATGTTGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATTGATAGAAC  >  CP000730/1333658‑1333934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`