Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,372,946	G→T	A277D (GCT→GAT)	pbuG ←	NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,372,946	0	G	T	100.0%	108.8 / NA	33	A277D (GCT→GAT)	pbuG	NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/19);  total (14/19)

CACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAAT  >  CP000730/2372809‑2373082                                                                                                                                          |                                                                                                                                         cacCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCaa                                                                                                                                       >  1:30633/1‑141 (MQ=255)    cTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAg                                                                                                                                    <  1:47843/141‑1 (MQ=255)       aaGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGcta                                                                                                                                 <  2:220746/141‑1 (MQ=255)              ttCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGt                                                                                                                          >  1:246163/1‑141 (MQ=255)                    ataAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGc                                                                                                                    <  2:179209/141‑1 (MQ=255)                        gaagTCGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTa                                                                                                                <  2:322921/141‑1 (MQ=255)                             tgttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCaaa                                                                                                           <  2:395474/141‑1 (MQ=255)                             tgttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCaaa                                                                                                           >  1:79972/1‑141 (MQ=255)                              tttgtAGTTGGTCCAAAAACTGAGCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAg                                                                                                          <  2:33879/140‑1 (MQ=255)                              gttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAg                                                                                                          <  1:182739/141‑1 (MQ=255)                              gttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAg                                                                                                          >  1:367732/1‑141 (MQ=255)                               ttgtAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGa                                                                                                         <  2:54885/141‑1 (MQ=255)                                  tAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAAt                                                                                                      <  1:94896/141‑1 (MQ=255)                                                  tGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGt                                                                                      <  2:30633/141‑1 (MQ=255)                                                   gATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTAGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGtt                                                                                     >  2:30830/1‑141 (MQ=255)                                                         aCAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATa                                                                               >  1:16555/1‑141 (MQ=255)                                                           aaTCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAAc                                                                             <  1:21151/141‑1 (MQ=255)                                                               gTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAAtt                                                                         <  1:89781/141‑1 (MQ=255)                                                                    tAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGaa                                                                    <  2:200646/141‑1 (MQ=255)                                                                        gAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGt                                                                <  2:269237/141‑1 (MQ=255)                                                                        gAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACATGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGt                                                                >  1:7071/1‑141 (MQ=255)                                                                              gagaACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGtt                                                          <  1:233951/141‑1 (MQ=255)                                                                                       gCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGa                                                 <  2:248856/141‑1 (MQ=255)                                                                                          ctACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACtt                                              <  2:246163/141‑1 (MQ=255)                                                                                                      ggTAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAAc                                  <  1:117366/141‑1 (MQ=255)                                                                                                        tAATTTATTATCTTTCATAATACCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGc                                >  1:217457/1‑141 (MQ=255)                                                                                                                        ataataCCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCg                >  1:118194/1‑141 (MQ=255)                                                                                                                         taataCCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGa               <  1:120115/141‑1 (MQ=255)                                                                                                                         taataCCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGa               >  2:296328/1‑141 (MQ=255)                                                                                                                             aCCTGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGt           >  2:246302/1‑141 (MQ=255)                                                                                                                                tGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTgg        >  2:73608/1‑141 (MQ=255)                                                                                                                                tGCTTGAGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTgg        >  2:73609/1‑141 (MQ=255)                                                                                                                                      aGTATCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAAt  >  1:284859/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                         CACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAAAATAACAATTAAGAACTGTATCGTTAATAATTGACTTGGGTCTTTAAACGCTTCAAATGCAGCACCGAACGTTGGTTCAAT  >  CP000730/2372809‑2373082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑