Predicted mutation
evidence seq id position mutation freq annotation gene description
JC CP000730 353,691 Δ3 bp 5.7% intergenic (+127/‑24) USA300HOU_0322 → / → USA300HOU_0323 hypothetical protein/hypothetical membrane protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 353694 =89 (1.000)5 (0.060) 5/236 NT 5.7% intergenic (+130/‑23) USA300HOU_0322/USA300HOU_0323 hypothetical protein/hypothetical membrane protein
?CP000730 = 353690 85 (1.050)intergenic (+126/‑27) USA300HOU_0322/USA300HOU_0323 hypothetical protein/hypothetical membrane protein

ACTGTTGTTGACACATCTGTAGTCATTACACCTGATGCTGCTAAAACCATCAATAGTAAACTAAATACCGTTGCTAAACCCTGAACAACAATTCCTATTATTGTCATTATTTTGATCGATTTTTCCATTGTCTTACTCCTTTATTATTATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/353844‑353694
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttattattattTACAAACTTGCGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTGAAACTGGTTAAAAGTTTTAAAGAAAGCCTATGTTAATCTTTTT  <  CP000730/353690‑353554
                                                                                                                                                                                                                                                                                                
ACTGTTGTTGACACATCTGTAGTCATTACACCTGATGCTGCTAAAACCATCAATAGTAAACTAAATACCGTTGCTAAACCCTGAACAACAATTCCTATTATTGTCATTATTTTGATCGATTTTTCCATTGTCTTACTCCTT                                                                                                                                                     <  2:936235/141‑1
    TTGTTGACACATCTGTAGTCATTACACCTGATGCTGCTAAAACCATCAATAGTAAACTAAATACCGTTGCTAAACCCTGAACAACAATTCCTATTATTGTCATTATTTTGATCGATTTTTCCATTGTCTTACTCCTTTATT                                                                                                                                                 >  2:74403/1‑141
      GTTGACACATCTGTAGTCATTACACCTGATGCTGCTAAAACCATCAATAGTAAACTAAATACCGTTGCTAAACCCTGAACAACAATTCCTATTATTGTCATTATTTTGATCGATTTTTCCATTGTCTTACTCCTTTATTAT                                                                                                                                               <  1:937030/141‑1
      GTTGACACATCTGTAGTCATTACACCTGATGCTGCTAAAACCATCAATAGTAAACTAAATACCGTTGCTAAACCCTGAACAACAATTCCTATTATTGTCATTATTTTGATCGATTTTTCCATTGTCTTACTCCTTTATTAT                                                                                                                                               <  1:580879/141‑1
       TTGACACATCTGTAGTCATTACACCTGATGCTGCTAAAACCATCAATAGTAAACTAAATACCGTTGCTAAACCCTGAACAACAATTCCTATTATTGTCATTATTTTGATCGATTTTTCCATTGTCTTACTCCTTTATTATT                                                                                                                                              >  2:1062600/1‑141
       TTGACACATCTGTAGTCATTACACCTGATGCTGCTAAAACCATCAATAGTAAACTAAATACCGTTGCTAAACCCTGAACAACAATTCCTATTATTGTCATTATTTTGATCGATTTTTCCATTGTCTTACTCCTTTATTATT                                                                                                                                              <  1:161209/141‑1
                                                                     GTTGCTAAACCCTGAATAACAATTCCTATTATTGTCATTATTTTGATCGATTTTTCCATTGACTTACTCCTTTATTATTATTTACAAACTTACGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTT                                                                                <  2:365681/141‑1
                                                                            AACCCTGAATAACAAGTCCTATTATTGTCATTATTTTGATCGATTTTTCCATTGACTTACTCCTTTATTATTATTTACAAACTTACGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATT                                                                         <  1:903122/141‑1
                                                                                                                   TCGATTTTTCCATTGTCTTACTCCTTTATTATTATTTACAAACTTGCGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTGAAACTGGTTAA                                  <  1:450387/141‑1
                                                                                                                            CCATTGACTTACTCCTTTATTATTATTTACAAACTTACGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTGAAACTGGTTAAAAGTTTTAA                         >  2:525842/1‑141
                                                                                                                                 gaCTTACTCCTTTATTATTATTTACAAACTTACGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTGAAACTGGTTAAAAGTTTTAAAGAAA                    <  2:126495/139‑1
                                                                                                                                             TATTATTATTTACAAACTTGCGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTGAAACTGGTTAAAAGTTTTAAAGAAAGCCTATGTTAAT        <  2:68701/141‑1
                                                                                                                                              ATTATTATTTACAAACTTGCGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTGAAACTGGTTAAAAGTTTTAAAGAAAGCCTATGTTAATC       >  1:735726/1‑141
                                                                                                                                                TATTATTTACAAACTTGCGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTGAAACTGGTTAAAAGTTTTAAAGAAAGCCTATGTTAATCTT     >  2:1010434/1‑141
                                                                                                                                                 ATTATTTACAAACTTGCGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTTAATCTGTTTAAAAGTTTTAAAGAAAGCCTATGTTAATCTTT    >  2:548259/1‑141
                                                                                                                                                 ATTATTTACAAACTTGCGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTGAAACTGGTTAAAAGTTTTAAAGAAAGCCTATGTTAATCTTT    >  2:263354/1‑141
                                                                                                                                                   TATTTACAAACTTGCGTTTGTGTTCTTATATATATATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTGAAACTGGTTAAAAGTTTTAAAGAAAGCCTATGTTAATCTTTTT  <  1:1007571/141‑1
                                                                                                                                                                                                                                                                                                
ACTGTTGTTGACACATCTGTAGTCATTACACCTGATGCTGCTAAAACCATCAATAGTAAACTAAATACCGTTGCTAAACCCTGAACAACAATTCCTATTATTGTCATTATTTTGATCGATTTTTCCATTGTCTTACTCCTTTATTATTATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/353844‑353694
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttattattattTACAAACTTGCGTTTGTGTTCTTATATATAAATGTTTAAATTTATATCGTGTTTATTTTAAAAATTATTGTCGCTAAAGCTCATTTACTTTTTGAAACTGGTTAAAAGTTTTAAAGAAAGCCTATGTTAATCTTTTT  <  CP000730/353690‑353554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.