BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	453,608	T→C	5.9%	L116L (TTA→CTA)	set22 →	staphylococcal exotoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	453,608	0	T	C	5.9%	284.4 / 5.1	98	L116L (TTA→CTA)	set22	staphylococcal exotoxin
Reads supporting (aligned to +/- strand): ref base T (46/45); new base C (3/3); total (50/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTAACGTTGTACGCTTTAACCAAGAAAATCAAAATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGTTTCACT  >  CP000730/453470‑453747

tCTAACGTTGTACGCTTTAACCAAGAAAATCAAAATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTa                                                                                                                                           <  2:635733/141‑1 (MQ=255)

   aaCGTTGTACGCTTTAACCAAGAAAATCAAAATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCt                                                                                                                                        >  1:453132/1‑141 (MQ=255)

       ttGTACGCTTTAACCAAGAAAATCAGAACCACCAATTATTCTCATTAGGTAAAGATAAAGAGACATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTCGTAAAAGAATTAAGTGATCCAAAAGGAAGATTATCTACTg                                                                                                                                    >  2:71394/1‑141 (MQ=255)

            cGCTTTAACCAAGAAAATCAAAATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTtggt                                                                                                                               >  2:264727/1‑141 (MQ=255)

            cGCTTTAACCAAGAAAATCAAAATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCGTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTtggt                                                                                                                               >  1:56998/1‑141 (MQ=255)

               tttAACCAAGAAAATCAAAATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTggtggt                                                                                                                            >  1:904786/1‑141 (MQ=255)

                       aGAAAATCAAAATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTaa                                                                                                                    <  1:965173/141‑1 (MQ=255)

                       aGAAAATCAAAATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTaa                                                                                                                    <  1:507466/141‑1 (MQ=255)

                        gAAAATCAAAATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAg                                                                                                                   >  2:697147/1‑141 (MQ=255)

                            atcaaaatcaCCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGaaaa                                                                                                               >  2:487617/1‑141 (MQ=255)

                             tcaaaatcaCCAATTATTCTTATTAGGTAAAGTTAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGACTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTATaaaaaa                                                                                                              >  2:938476/1‑141 (MQ=255)

                               aaaatcaCCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATa                                                                                                            >  2:423407/1‑141 (MQ=255)

                                aaatcaCCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATaa                                                                                                           <  1:110464/141‑1 (MQ=255)

                                aaaacaACAATTATTATTATTAGGTAAAGAAAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGAGGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGAAACAAAAAAAAATaa                                                                                                           <  2:899452/141‑1 (MQ=255)

                                 aatcaCCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAAc                                                                                                          >  1:695734/1‑141 (MQ=255)

                                  atcaCCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACa                                                                                                         >  1:1016125/1‑141 (MQ=255)

                                  atcaCCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACa                                                                                                         >  1:985439/1‑141 (MQ=255)

                                    caCCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACaaa                                                                                                       >  2:890213/1‑141 (MQ=255)

                                     aCCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAAt                                                                                                      >  1:1057122/1‑141 (MQ=255)

                                     aCCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAAt                                                                                                      >  2:655796/1‑141 (MQ=255)

                                     aCCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAAt                                                                                                      <  2:522541/141‑1 (MQ=255)

                                      ccAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAAtc                                                                                                     >  1:789949/1‑141 (MQ=255)

                                      ccAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAAtc                                                                                                     >  2:132283/1‑141 (MQ=255)

                                      ccAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAAtc                                                                                                     <  2:653235/141‑1 (MQ=255)

                                         attattCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAAtcttc                                                                                                  <  2:989378/141‑1 (MQ=255)

                                         attattCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAAtcttc                                                                                                  >  1:333445/1‑141 (MQ=255)

                                          ttattCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAAtcttct                                                                                                 <  1:346519/141‑1 (MQ=255)

                                           tattCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTg                                                                                                <  2:662003/141‑1 (MQ=255)

                                             ttCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGaa                                                                                              >  1:755413/1‑141 (MQ=255)

                                               cTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAAc                                                                                            >  1:750968/1‑141 (MQ=255)

                                                 tattaGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACtt                                                                                          <  1:1030299/141‑2 (MQ=255)

                                                  attaGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTaa                                                                                         <  1:130685/141‑1 (MQ=255)

                                                     aGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATac                                                                                      <  1:760552/141‑1 (MQ=255)

                                                      ggTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATaca                                                                                     <  1:494975/141‑1 (MQ=255)

                                                      ggTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATaca                                                                                     >  2:114913/1‑141 (MQ=255)

                                                       gTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATacac                                                                                    <  2:765111/141‑1 (MQ=255)

                                                       gTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATacac                                                                                    <  1:134197/141‑1 (MQ=255)

                                                        taaagataaagaGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATAcaca                                                                                   <  2:730305/141‑1 (MQ=255)

                                                            gataaagaGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACattta                                                                               >  1:619921/1‑141 (MQ=255)

                                                              taaagaGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACatttatt                                                                             <  1:494390/141‑1 (MQ=255)

                                                              taaagaGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACatttatt                                                                             <  2:430081/141‑1 (MQ=255)

                                                               aaagaGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGAAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACatttattt                                                                            <  2:782121/141‑1 (MQ=255)

                                                                aagaGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTg                                                                           >  1:308916/1‑141 (MQ=255)

                                                                aagaGAAATATAAAGAAAGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTAAATAAAAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTg                                                                           <  2:696602/141‑1 (MQ=255)

                                                                 agagAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGt                                                                          <  2:299300/141‑1 (MQ=255)

                                                                    gaAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTtaa                                                                       >  1:698962/1‑141 (MQ=255)

                                                                    gaAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTtaa                                                                       >  2:523310/1‑141 (MQ=255)

                                                                       atatAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTACTTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTtaataa                                                                    <  2:957802/141‑1 (MQ=255)

                                                                         ataAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAg                                                                  <  2:783015/141‑1 (MQ=255)

                                                                         ataAAAAAGGCCTTGAAGGCCAGAATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGACTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAg                                                                  >  2:463685/1‑141 (MQ=255)

                                                                                  gCATTGAAGGCAACGCTGTCTTTGTGGTAAAAGAATTAATTCATCCAAACGGAAGATCAACTCCAGTTGGAAGTCTGCCTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCg                                                         <  1:647603/141‑1 (MQ=255)

                                                                                  gCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCg                                                         <  2:810471/141‑1 (MQ=255)

                                                                                   cATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGAGAAAAAAAAAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCgg                                                        <  2:425124/141‑1 (MQ=255)

                                                                                    aTTTAAGGCAAAGATGGCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGACTAGCTATGGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGa                                                       <  1:783098/141‑1 (MQ=255)

                                                                                     ttgaaggccagaaTGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGACTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGaa                                                      >  2:698587/13‑141 (MQ=255)

                                                                                      tGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGaaa                                                     >  1:325355/1‑141 (MQ=255)

                                                                                      tGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGaaa                                                     <  2:519509/141‑1 (MQ=255)

                                                                                      tGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGaaa                                                     >  1:853018/1‑141 (MQ=255)

                                                                                        aaGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAAtt                                                   >  2:927451/1‑141 (MQ=255)

                                                                                        aaGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAAtt                                                   <  1:941661/141‑1 (MQ=255)

                                                                                           gCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAg                                                <  1:890213/141‑1 (MQ=255)

                                                                                            cAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGa                                               <  2:1008088/141‑1 (MQ=255)

                                                                                             aaaGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGGTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAACTGCAGGGCGGAAAtaaagaa                                              >  2:318753/1‑135 (MQ=255)

                                                                                              aaGATGTCTTTGTGGTAAAAGAATTAAGTGAACCAAAGGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATg                                             >  2:323193/1‑141 (MQ=255)

                                                                                              aaGATGTCTATGTGGTAAAAGCATTAATACATCCAGACGTCAGTATATCTACTGTTGGTGGTGTGAATAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATg                                             >  2:322977/1‑141 (MQ=255)

                                                                                               aGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGc                                            <  1:156085/141‑1 (MQ=255)

                                                                                                 aTGTCTTTGTGGTAAAAGACTTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCAt                                          >  2:816708/1‑141 (MQ=255)

                                                                                                    tCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCaa                                       >  2:996730/1‑141 (MQ=255)

                                                                                                      tttGTGGTAAAAGAATTAATTGATCCAAACGGTAGACTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATg                                                            <  1:70319/118‑1 (MQ=255)

                                                                                                      tttGTGGTAAAAGAATTAATTGATCCAAACGGTAGACTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATg                                                            >  2:70319/1‑118 (MQ=255)

                                                                                                       ttGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTg                                    <  1:390810/141‑1 (MQ=255)

                                                                                                          tgGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACt                                 <  1:33738/141‑1 (MQ=255)

                                                                                                           ggTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTc                                <  2:776816/141‑1 (MQ=255)

                                                                                                           ggTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTc                                <  1:418694/141‑1 (MQ=255)

                                                                                                             tAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCAt                              <  2:724919/141‑1 (MQ=255)

                                                                                                              aaaaGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCAtt                             <  2:750968/141‑1 (MQ=255)

                                                                                                                aaGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCAtttt                           <  1:709498/141‑1 (MQ=255)

                                                                                                                  aaattaattGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCa                         <  2:1006867/140‑1 (MQ=255)

                                                                                                                   aattaattGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCaa                        >  2:562112/1‑141 (MQ=255)

                                                                                                                     ataattGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATGTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAAtt                      >  1:1028804/2‑141 (MQ=255)

                                                                                                                       aattGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATtaa                    >  1:1073207/1‑141 (MQ=255)

                                                                                                                           gATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATaaa                >  1:930106/1‑141 (MQ=255)

                                                                                                                            aTCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAaag               <  2:172347/141‑1 (MQ=255)

                                                                                                                            aTCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCAGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAaag               >  2:266551/1‑141 (MQ=255)

                                                                                                                              ccAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAaagaa             <  2:330184/141‑1 (MQ=255)

                                                                                                                                 aaCGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAaagaagaa          >  1:1052263/1‑141 (MQ=255)

                                                                                                                                   cGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAACCTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTCAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGt        >  2:931895/1‑141 (MQ=255)

                                                                                                                                   cGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGt        >  1:921164/1‑141 (MQ=255)

                                                                                                                                   cGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGt        <  1:923343/141‑1 (MQ=255)

                                                                                                                                   cGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGt        >  1:476320/1‑141 (MQ=255)

                                                                                                                                   cGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTAGGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGt        <  1:836357/141‑1 (MQ=255)

                                                                                                                                    ggTAGACTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTTAATTAATAAAGAAGAAGtt       <  1:463685/141‑1 (MQ=255)

                                                                                                                                        gATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGTTTCAc   >  1:388786/1‑141 (MQ=255)

                                                                                                                                         aTTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGTTTCACt  >  2:312744/1‑141 (MQ=255)

                                                                                                                                         aTTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGTTTCACt  >  2:526213/1‑141 (MQ=255)

                                                                                                                                         aTTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGTTTCACt  <  1:927451/141‑1 (MQ=255)

                                                                                                                                         aTTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGTTTCACt  >  2:601656/1‑141 (MQ=255)

                                                                                                                                         aTTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGCTTCACt  >  1:472057/1‑141 (MQ=255)

TCTAACGTTGTACGCTTTAACCAAGAAAATCAAAATCACCAATTATTCTTATTAGGTAAAGATAAAGAGAAATATAAAGAAGGCATTGAAGGCAAAGATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGATTATCTACTGTTGGTGGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAAGTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTCAATTAATAAAGAAGAAGTTTCACT  >  CP000730/453470‑453747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`