BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	464,599	G→A	5.9%	P92P (CCG→CCA)	USA300HOU_0439 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	464,599	0	G	A	5.9%	312.2 / 5.4	101	P92P (CCG→CCA)	USA300HOU_0439	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (53/41); new base A (2/4); total (56/45)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

Only 100 of 104 total aligned reads displayed.

TTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGAAAAACA  >  CP000730/464459‑464738

tttGAGAAAACATGAGATATGTATCCAATAAAGAATCGCGAGGACTTATATGAAAAAGAAGGATATCGAGATAGAGAAAAAAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCg                                                                                                                                             <  2:1068148/141‑1 (MQ=11)

 ttGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCgg                                                                                                                                            >  1:1027006/1‑141 (MQ=35)

  tGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCggg                                                                                                                                           >  1:689502/1‑141 (MQ=35)

      aaaaCATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTa                                                                                                                                       <  1:380957/141‑1 (MQ=35)

        aaCATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTAtt                                                                                                                                     <  2:1018312/141‑1 (MQ=35)

         aCATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTa                                                                                                                                    <  2:546705/141‑1 (MQ=37)

               gATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAAt                                                                                                                              >  1:657921/1‑141 (MQ=40)

                atatGTATCCAATCAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGCTAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGCTTTCGCCCAAAGTAATAAACCGGGTGTATTCGATAATg                                                                                                                             >  2:583111/1‑141 (MQ=11)

                   tGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAg                                                                                                                          >  1:779996/1‑141 (MQ=40)

                    gTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAgg                                                                                                                         >  2:297427/1‑141 (MQ=40)

                        ccAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATg                                                                                                                     >  2:839507/1‑141 (MQ=255)

                        ccAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATg                                                                                                                     >  1:235158/1‑141 (MQ=40)

                          aatcaaaaaTCTCGAGGACCTATATGATAAAGAAGGAGATCGAGATGGCGAATTTAAAAAAGGTGACAAAGGGATGTGGACGATTTACACAGATTTCGCCAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATggt                                                                                                                   <  2:29586/134‑3 (MQ=11)

                          aaTTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAt                                                                                                                   <  1:201087/141‑1 (MQ=255)

                           aTTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAtt                                                                                                                  <  1:560166/141‑1 (MQ=255)

                           aTTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAtt                                                                                                                  <  1:706865/141‑1 (MQ=255)

                           aTTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAtt                                                                                                                  <  2:437822/141‑1 (MQ=255)

                             tAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAtttt                                                                                                                <  1:172201/141‑1 (MQ=255)

                             tAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGAtttt                                                                                                                <  1:423789/141‑1 (MQ=255)

                              aaGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTa                                                                                                               >  1:203775/1‑141 (MQ=255)

                               aGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGCTAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTaa                                                                                                              <  1:802677/141‑1 (MQ=255)

                                  aTCTCGAGGACTTATATGACGAGGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAAtt                                                                                                           <  2:662984/141‑1 (MQ=255)

                                  aTCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAAtt                                                                                                           >  2:887942/1‑141 (MQ=255)

                                  aTCTCGAGGACCTATATGATAAAGAAGGATATCGAGATGGCGAATTTAAAAAAGGTGACAAAGGGATGTGGACGATTTACACAGATTTCGCCAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATGGTTTTAAAtt                                                                                                           >  1:63482/1‑141 (MQ=11)

                                     tcGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTgg                                                                                                        >  1:963622/1‑141 (MQ=255)

                                       gAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGAt                                                                                                      >  1:494594/1‑141 (MQ=255)

                                        aGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATa                                                                                                     <  2:1041529/141‑1 (MQ=255)

                                        aGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATa                                                                                                     >  2:158862/1‑141 (MQ=255)

                                         ggACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAg                                                                                                    >  2:416426/1‑141 (MQ=255)

                                           aCTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGaa                                                                                                  >  1:236681/1‑141 (MQ=255)

                                              tataTGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATa                                                                                               <  1:620810/141‑1 (MQ=255)

                                               atatGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATac                                                                                              >  1:294706/1‑141 (MQ=255)

                                               atatGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATac                                                                                              <  1:921901/141‑1 (MQ=255)

                                                  tgataaaGAAGGATATCGAGATGGCGAATTTAAAAAAGGTGACAAAGGGATGTGGACGATTTACACAGATTTCGCCAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATGGTTTTAAATTTGGATAGAAATACACg                                                                                           <  2:278741/137‑1 (MQ=14)

                                                  tGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACg                                                                                           <  1:127228/141‑1 (MQ=255)

                                                   gataaaGAAGGATATCGAGATGGCGAATTTAAAAAAGGTGACAAAGGGATGTGGACGATTTACACAGATTTCGCCAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATGGTTTTAAATTTGGATAGAAATACACga                                                                                          <  1:146621/138‑2 (MQ=14)

                                                    aCAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACGCGTa                                                                                         >  2:526887/1‑141 (MQ=255)

                                                        agaagGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAGTGATGGTATGATTTTAAATTTGGATAGAAATACACATACGGc                                                                                     >  2:1038806/1‑141 (MQ=255)

                                                         gaagGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAAGAAACCTGGTGGATGAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGcc                                                                                    >  1:319186/1‑141 (MQ=255)

                                                            ggATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAg                                                                                 >  1:577650/1‑141 (MQ=255)

                                                             gATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAgg                                                                                >  2:212276/1‑141 (MQ=255)

                                                              atatCGAGCGAGAGACATTATAAAGGGTGCAAAAGGGATGTGGATGATGTATTCAGATTTCGCCAAAAGTAATGAACCGGGTGTATTAAATACTGACGGTATGATGTGGAACTTGGATAGAAATACAAGTACGGCCACggg                                                                               >  2:226255/1‑141 (MQ=12)

                                                               tatCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAgggc                                                                              >  1:546414/1‑140 (MQ=255)

                                                               tatCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGa                                                                              <  2:230217/141‑1 (MQ=255)

                                                               tatCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGa                                                                              >  2:943055/1‑141 (MQ=255)

                                                                atCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGat                                                                             <  1:416426/141‑1 (MQ=255)

                                                                atCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGat                                                                             >  1:460827/1‑141 (MQ=255)

                                                                 tCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGata                                                                            >  2:599077/1‑141 (MQ=255)

                                                                 tCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGata                                                                            <  2:715156/141‑1 (MQ=255)

                                                                  cGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGatat                                                                           <  2:96860/141‑1 (MQ=255)

                                                                     gaTAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGAtattat                                                                        >  1:976333/1‑141 (MQ=255)

                                                                       tAGCTAATATAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTATATAAGGAAGGTATTATTGGAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTAttt                                                                      <  2:914722/141‑1 (MQ=21)

                                                                        ggCGAATTTAAAAAAGGTGACAAAGGGATGTGGACGATTTACACAGATTTCGCCAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATGGTTTTAAATTTGGATAGAAATACACGAACGGCCAAGGGATATTAtttt                                                                     <  2:239550/140‑1 (MQ=18)

                                                                        aGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTAtttt                                                                     >  1:768538/1‑141 (MQ=255)

                                                                          cGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGt                                                                   <  2:560078/141‑1 (MQ=255)

                                                                             aTTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGa                                                                >  2:1011315/1‑141 (MQ=255)

                                                                              tttAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGAt                                                               >  2:896020/1‑141 (MQ=255)

                                                                                tAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATAc                                                             <  2:570421/141‑1 (MQ=255)

                                                                                tAAAAAAGGTGACAAAGAGATGTGGATGATATATACAGATTTAGACAAAAGAAATCATTCGGCTGTTTTGGAAAATGAAGGTATGCTTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATAc                                                             <  1:542214/141‑1 (MQ=255)

                                                                                 aaaaaaGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTAGTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGACATTATTTTGTAGATACt                                                            >  1:384309/1‑141 (MQ=255)

                                                                                  aaaaaGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACta                                                           >  1:726845/1‑141 (MQ=255)

                                                                                  aaaaaGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACta                                                           >  2:426262/1‑141 (MQ=255)

                                                                                    aaaGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACtata                                                         >  1:597303/1‑141 (MQ=255)

                                                                                    aaaGGTGACAAAGGGAAGTGGATGATATATACAGATTTAGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTTGAGAAAAATAAACGAACGGCGAAGGGATATTATTTTGTAGATACtaaa                                                         >  2:84596/1‑139 (MQ=21)

                                                                                       ggTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTatatat                                                      <  2:747606/141‑1 (MQ=255)

                                                                                         tGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGa                                                    <  2:63758/141‑1 (MQ=255)

                                                                                          gACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGAc                                                   >  1:389279/1‑141 (MQ=255)

                                                                                          gACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGAc                                                   >  2:506568/1‑141 (MQ=255)

                                                                                          gACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGAc                                                   >  1:727708/1‑141 (MQ=255)

                                                                                            cAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACaa                                                 >  2:482676/1‑141 (MQ=255)

                                                                                               aGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCa                                              <  2:995216/141‑1 (MQ=255)

                                                                                               aGGGATGTGGATGATATATACAGATTTCGACAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCa                                              <  2:117351/141‑1 (MQ=255)

                                                                                                 ggATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATg                                            <  1:726318/141‑1 (MQ=255)

                                                                                                  gatgagaataaaatataCAGATTTCGCCAAAAGTAATAAACGGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAAAACAAGTACAGCCAAGTGTTATTATTTTGTAGATACTATATATGACAATCATGa                                           <  2:648726/129‑1 (MQ=25)

                                                                                                  gATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGa                                           <  2:364276/141‑1 (MQ=255)

                                                                                                   aTGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGaa                                          >  1:662567/1‑141 (MQ=255)

                                                                                                   aTGTGGATGATATATACAGATTTCGACAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGaa                                          <  2:583078/141‑1 (MQ=255)

                                                                                                      tgGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAAc                                       >  2:661428/1‑141 (MQ=255)

                                                                                                      tgGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAAc                                       >  1:877558/1‑141 (MQ=255)

                                                                                                      tgGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAAc                                       >  1:197223/1‑141 (MQ=255)

                                                                                                         atgatATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAActct                                    <  1:198234/141‑1 (MQ=255)

                                                                                                         atgatATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTAATAGATAAAGAAGGTATGATCTAAAATTTGTGCAGCAATTCACGGACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAActct                                    >  1:1028898/1‑141 (MQ=37)

                                                                                                              atataCAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAg                               >  1:239063/1‑141 (MQ=255)

                                                                                                              atataCAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAg                               <  1:271465/141‑1 (MQ=255)

                                                                                                               tataCAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGt                              <  2:195877/141‑1 (MQ=255)

                                                                                                               tataCAGACTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGt                              <  1:171359/141‑1 (MQ=255)

                                                                                                                  aCAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTaaa                           >  2:755061/1‑141 (MQ=255)

                                                                                                                       tttCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATta                      <  2:1001192/141‑1 (MQ=255)

                                                                                                                       tttCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATta                      <  1:769098/141‑1 (MQ=255)

                                                                                                                         tCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATtata                    >  1:856371/1‑141 (MQ=255)

                                                                                                                         tCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATtata                    >  1:747997/1‑141 (MQ=255)

                                                                                                                             cAAAAGTAATAGACCAGGTGTATTAGATAATGAAGGTATGGTTTTAAATTTGGATAGAAATACACGAACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGt                >  1:173025/1‑141 (MQ=38)

                                                                                                                             cAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGt                >  1:1000517/1‑141 (MQ=255)

                                                                                                                                    aataaACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGAta         >  2:23093/1‑140 (MQ=255)

                                                                                                                                    aataaACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATAATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATg         <  2:250849/141‑1 (MQ=255)

                                                                                                                                    aataaAACGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAAAAAAAAAAAAAAAAATTATAGAGTTGAGATg         <  2:5017/141‑1 (MQ=255)

                                                                                                                                     ataaACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGa        >  1:688957/1‑141 (MQ=255)

                                                                                                                                      taaACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGaa       >  1:858620/1‑141 (MQ=255)

                                                                                                                                       aaaCCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGGTGAGATGaaa      >  2:941145/1‑141 (MQ=255)

                                                                                                                                           cGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGAAAAACa  >  2:247607/1‑141 (MQ=255)

TTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGACTTATATGACAAAGAAGGATATCGAGATAGCGAATTTAAAAAAGGTGACAAAGGGATGTGGATGATATATACAGATTTCGCCAAAAGTAATAAACCGGGTGTATTAGATAATGAAGGTATGATTTTAAATTTGGATAGAAATACACGTACGGCCAAGGGATATTATTTTGTAGATACTATATATGACAATCATGAAAACTCTTATAGTAAAAATTATAGAGTTGAGATGAAAAACA  >  CP000730/464459‑464738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`