BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	491,302	G→C	7.8%	intergenic (+60/‑272)	metQ → / → sle1	ABC superfamily ATP binding cassette transporter, binding protein/possible LysM family autolysin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	491,302	0	G	C	7.8%	324.2 / 14.8	103	intergenic (+60/‑272)	metQ/sle1	ABC superfamily ATP binding cassette transporter, binding protein/possible LysM family autolysin
Reads supporting (aligned to +/- strand): ref base G (50/42); new base C (6/2); total (56/47)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TCAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGA  >  CP000730/491169‑491435

tCAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATaaaa                                                                                                                                <  1:286100/141‑1 (MQ=255)

 cAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAat                                                                                                                               <  1:339586/141‑1 (MQ=255)

  aaaGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAatt                                                                                                                              >  1:226538/1‑141 (MQ=255)

    aGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTG‑AGGGCGTTATTGTTGGA‑AATaattttattc                                                                                                                          <  2:1026428/141‑9 (MQ=255)

     gATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAattatt                                                                                                                           >  1:496483/1‑141 (MQ=255)

      aTGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTAttt                                                                                                                          <  1:939655/141‑1 (MQ=255)

         gagagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCa                                                                                                                       >  2:482984/1‑141 (MQ=255)

           gagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAAt                                                                                                                     >  1:460150/1‑141 (MQ=255)

           gagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAAt                                                                                                                     >  1:639352/1‑141 (MQ=255)

           gagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAAt                                                                                                                     >  1:678601/1‑141 (MQ=255)

           gagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAAt                                                                                                                     >  2:283128/1‑141 (MQ=255)

             gaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATag                                                                                                                   <  2:1052677/141‑1 (MQ=255)

             gaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATag                                                                                                                   <  2:404141/141‑1 (MQ=255)

                 ccTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGaa                                                                                                               >  2:1042017/1‑141 (MQ=255)

                   tGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGTCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAg                                                                                                             >  1:102537/1‑141 (MQ=255)

                    gTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAgg                                                                                                            >  1:549361/1‑141 (MQ=255)

                    gTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAgg                                                                                                            >  2:358131/1‑141 (MQ=255)

                        aTTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGAtt                                                                                                        <  2:964616/141‑1 (MQ=255)

                             tCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAAt                                                                                                   <  2:940976/141‑1 (MQ=255)

                              cTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAAtt                                                                                                  >  2:409234/1‑140 (MQ=255)

                              cTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATc                                                                                                  <  1:61154/141‑1 (MQ=255)

                              cTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATc                                                                                                  >  2:342731/1‑141 (MQ=255)

                               tAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCa                                                                                                 <  1:1065523/141‑1 (MQ=255)

                                     tGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTtat                                                                                           <  1:593423/141‑1 (MQ=255)

                                     tGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTtat                                                                                           <  2:905119/141‑1 (MQ=255)

                                     tGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTtat                                                                                           <  2:905027/141‑1 (MQ=255)

                                      gagaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTtata                                                                                          >  1:518105/1‑141 (MQ=255)

                                       agaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAg                                                                                         >  2:718791/1‑141 (MQ=255)

                                        gaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGt                                                                                        >  2:280327/1‑141 (MQ=255)

                                        gaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGt                                                                                        >  2:763428/1‑141 (MQ=255)

                                          ttAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTCGGGCGTTATTGTTGGAGACTAAAATTCTTTCCAATAGAGAAGGGGATTGTAATCATTTTATAGTGa                                                                                      <  1:973687/141‑1 (MQ=255)

                                          ttAAAGAAATAGAAACGTCATTAGCAAAATAAATAATATTGCATACAACAAGCAAAGTTCATGCTTATGTTAGTCGGAAGATAATGTTGGAGCCTACAATTATTATCAATCCAGAAAGGGACTTAAATCATTTTATAGTGa                                                                                      <  1:973900/141‑1 (MQ=255)

                                               gCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAatat                                                                                 >  2:891724/1‑141 (MQ=255)

                                                  aTAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAAtattat                                                                              <  2:886822/141‑1 (MQ=255)

                                                   tAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATg                                                                             >  1:111236/1‑141 (MQ=255)

                                                   tAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATg                                                                             <  1:283128/141‑1 (MQ=255)

                                                     gAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAGTGAAATATTATGaa                                                                           >  1:1020180/1‑141 (MQ=255)

                                                        aCGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAAtt                                                                        >  2:757262/1‑141 (MQ=255)

                                                        aCGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAAtt                                                                        >  1:188079/1‑141 (MQ=255)

                                                         cGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTg                                                                       <  1:325299/141‑1 (MQ=255)

                                                         cGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTg                                                                       <  1:864893/141‑1 (MQ=255)

                                                           tCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGta                                                                     <  1:2711/141‑1 (MQ=255)

                                                           tCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGta                                                                     <  1:2717/141‑1 (MQ=255)

                                                            cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                    >  1:124287/1‑141 (MQ=255)

                                                            cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTACGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                    >  2:84968/1‑141 (MQ=255)

                                                             aTTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaat                                                                   >  1:532624/1‑141 (MQ=255)

                                                                aGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTaataat                                                                <  2:169965/141‑1 (MQ=255)

                                                                aGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAAGTGTaataat                                                                >  2:376722/1‑141 (MQ=255)

                                                                aGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATCTTTGTAGGGCGTTATTGTTGGATAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTaataat                                                                <  1:375912/141‑1 (MQ=255)

                                                                   aaaaTAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTa                                                             <  1:423623/141‑1 (MQ=255)

                                                                     aataaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGa                                                           >  2:409367/1‑141 (MQ=255)

                                                                       taaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGata                                                         >  1:306152/1‑141 (MQ=255)

                                                                       taaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGata                                                         >  2:1041924/1‑141 (MQ=255)

                                                                        aaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGatat                                                        <  1:768720/141‑1 (MQ=255)

                                                                          atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                      <  1:48734/141‑1 (MQ=255)

                                                                          atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                      >  2:733269/1‑141 (MQ=255)

                                                                           ttATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGt                                                     >  1:991631/1‑141 (MQ=255)

                                                                           ttATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGt                                                     >  1:573272/1‑141 (MQ=255)

                                                                              tatTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTaaa                                                  <  1:251540/141‑1 (MQ=255)

                                                                                ttGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAAt                                                >  1:952538/1‑141 (MQ=255)

                                                                                 tGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATc                                               <  1:269087/141‑1 (MQ=255)

                                                                                    gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGCACTATTATGAAATTGTAATAATTTAGATATTGTAACATCtca                                            >  1:264808/1‑139 (MQ=255)

                                                                                    gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                            >  1:66578/1‑141 (MQ=255)

                                                                                    gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                            >  1:862810/1‑141 (MQ=255)

                                                                                         aCAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGt                                       <  1:667591/141‑1 (MQ=255)

                                                                                            aGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGt                                    <  2:233195/141‑1 (MQ=255)

                                                                                              cAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaa                                  <  2:737375/141‑1 (MQ=255)

                                                                                               aaaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaat                                 <  1:351535/141‑1 (MQ=255)

                                                                                               aaaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaat                                 <  2:728635/141‑1 (MQ=255)

                                                                                                tatgtCATACTTATGTATGGATTGGGTAATTGTAGAAAAATAAAATTATTTCAAATAGAGAAAGAGATTGTAATCATTTTATAGTTAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                <  2:409072/137‑1 (MQ=255)

                                                                                                aaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                <  2:1082117/141‑1 (MQ=255)

                                                                                                aaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                >  1:878433/1‑141 (MQ=255)

                                                                                                aaGTTCATGCTTATGTTTGTAGGGCGTCATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATAGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                >  2:979254/1‑141 (MQ=255)

                                                                                                 aGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaataa                               <  2:469343/141‑1 (MQ=255)

                                                                                                  gTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTaataat                              >  1:717143/1‑141 (MQ=255)

                                                                                                    tCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAAttt                            <  2:71694/141‑1 (MQ=255)

                                                                                                     cATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAAtttt                           >  1:906408/1‑141 (MQ=255)

                                                                                                      aTGCTTATGTTTGTAGGGCGTTATTGTTTGACAAAATATTTATTTCCAATAGAGAAAGGGTATGTAATCATGTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                          <  2:300226/141‑1 (MQ=255)

                                                                                                      aTGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTTGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                          >  2:518177/1‑141 (MQ=255)

                                                                                                      aTGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                          >  1:764522/1‑141 (MQ=255)

                                                                                                       tGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATCATTTTaa                         >  2:314314/1‑141 (MQ=255)

                                                                                                        gCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATTGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAg                        >  2:618124/1‑141 (MQ=255)

                                                                                                        gCTTATGTTTGTAGGGCGTTATTGTCGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAg                        >  2:918170/1‑141 (MQ=255)

                                                                                                             tGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTa                   <  2:331611/141‑1 (MQ=255)

                                                                                                             tGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTa                   >  2:1069518/1‑141 (MQ=255)

                                                                                                               tttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAt                 <  1:1033491/141‑1 (MQ=255)

                                                                                                               tttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAt                 >  1:853895/1‑141 (MQ=255)

                                                                                                                ttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAtt                <  2:467546/141‑1 (MQ=255)

                                                                                                                 tGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATta               >  1:44937/1‑141 (MQ=255)

                                                                                                                 tGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATta               >  2:163126/1‑141 (MQ=255)

                                                                                                                  gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat              <  2:518105/141‑1 (MQ=255)

                                                                                                                  gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat              <  1:445895/141‑1 (MQ=255)

                                                                                                                  gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat              <  1:30043/141‑1 (MQ=255)

                                                                                                                   tAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtata             >  2:1070771/1‑141 (MQ=255)

                                                                                                                   tAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtata             >  2:960458/1‑141 (MQ=255)

                                                                                                                    aGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATaa            >  1:783673/1‑141 (MQ=255)

                                                                                                                     gggCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATaaa           >  1:154024/1‑141 (MQ=255)

                                                                                                                       gCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAAt         >  2:65865/1‑141 (MQ=255)

                                                                                                                            aTTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTtgat    <  2:629270/141‑1 (MQ=255)

                                                                                                                              tgttgGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTtgatga  <  1:995972/141‑1 (MQ=255)

TCAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGA  >  CP000730/491169‑491435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`