Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 495,436 C→T 5.9% A228T (GCA→ACA)  yibE ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730495,4360CT 5.9% 249.3 / 4.1 86A228T (GCA→ACA) yibEhypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (35/45);  new base T (2/3);  total (37/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

TTCATGCATACCACTAGCAATTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATGGCAATGGT  >  CP000730/495296‑495574
                                                                                                                                            |                                                                                                                                          
ttCATGCATACCACTAGCAATTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGc                                                                                                                                            <  1:174680/141‑1 (MQ=255)
 tCATGCATACCACTAGCAATTGTACTTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGcc                                                                                                                                           <  1:40244/141‑1 (MQ=255)
   aTGCATACCACTAGCAATTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCATTTACCATCTGCCaa                                                                                                                                         >  2:368976/1‑140 (MQ=255)
        tACCACTAGCAATTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTAGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCatatata                                                                                                                                    <  2:134953/141‑1 (MQ=255)
          ccACTAGCAATTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATg                                                                                                                                  <  1:155383/141‑1 (MQ=255)
           cACTAGCAATTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGa                                                                                                                                 <  1:223533/141‑1 (MQ=255)
           cACTAGCAATTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGa                                                                                                                                 <  2:700203/141‑1 (MQ=255)
            aCTAGCAATTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGAt                                                                                                                                <  2:617645/141‑1 (MQ=255)
             cTAGCAATTGTAGTTGCAACATCCATAATGGGGCCAAGTGAGCCTATTAACACTGGGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGAtt                                                                                                                               <  2:409043/141‑1 (MQ=255)
                gCAATTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATATTTCGGTGGTGAGGAGAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAAt                                                                                                                            >  1:486859/1‑141 (MQ=255)
                  aaTTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCCTATTTAATGCCTTTACCATATGCCATATATATGATTAAtta                                                                                                                          >  2:769044/1‑140 (MQ=255)
                   aTTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCt                                                                                                                         >  2:824374/1‑141 (MQ=255)
                   aTTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCt                                                                                                                         >  1:129530/1‑141 (MQ=255)
                    ttGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACAATCTGCCATATATATGATTAATTCTg                                                                                                                        >  1:740485/1‑141 (MQ=255)
                      gTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGtt                                                                                                                      >  2:553798/1‑141 (MQ=255)
                      gTAATTGCAACAGCCATAATAGCGCCAAGTGATCCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGtt                                                                                                                      <  2:364558/141‑1 (MQ=255)
                             cAACATCCATAATAGCGCCTAGTGAACCTATTAACACTGCGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCta                                                                                                               >  2:305494/1‑141 (MQ=255)
                             cAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCta                                                                                                               >  1:809078/1‑141 (MQ=255)
                              aaCATCAATAATAGCGCCAAGTGAACCTATTAGCACTGAGGCTAGTAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTaaaaatat                                                                                                              <  2:73013/141‑6 (MQ=255)
                               aCATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCtata                                                                                                             <  2:1076627/141‑1 (MQ=255)
                                 aTCCATAATAGCGCCAAGTGAACCTATTAAAACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACt                                                                                                           <  2:891905/141‑1 (MQ=255)
                                  tCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATACTGTTAAACCTATACTc                                                                                                          >  2:922124/1‑141 (MQ=255)
                                   ccATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCa                                                                                                         <  1:1042378/141‑1 (MQ=255)
                                   ccATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCa                                                                                                         <  2:984432/141‑1 (MQ=255)
                                   ccATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATAATCa                                                                                                         <  2:626333/141‑1 (MQ=255)
                                    cATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGTCATATATATGATTAATTCTGTTAAACCTATACTCaa                                                                                                        <  2:546655/141‑1 (MQ=255)
                                    cATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCaa                                                                                                        <  1:793675/141‑1 (MQ=255)
                                     ataataGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCaaa                                                                                                       <  1:737730/141‑1 (MQ=255)
                                       aataGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCaaaaa                                                                                                     >  1:291049/1‑141 (MQ=255)
                                           gcgcCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGtt                                                                                                 <  1:755293/141‑1 (MQ=255)
                                            cgcCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTc                                                                                                >  1:148023/1‑141 (MQ=255)
                                              ccAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCg                                                                                              >  2:869748/1‑141 (MQ=255)
                                                aaGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGAt                                                                                            >  2:987955/1‑141 (MQ=255)
                                                  gTGAACCTATTAACACAGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACAAAAAAAAAAAAAAGTTCCGATaa                                                                                          <  2:791231/141‑1 (MQ=255)
                                                      aCCTATTAACACTGAGGCTAGGGAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGCTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATCCTCAAAAAAGTTCCGATAATTGt                                                                                      >  2:144104/1‑141 (MQ=255)
                                                      aCCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAAAAAAAAAAAAAAAAAGTTCCGATAATTGt                                                                                      <  2:992986/141‑1 (MQ=255)
                                                       ccTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTa                                                                                     <  1:174459/141‑1 (MQ=255)
                                                       ccTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTa                                                                                     >  1:373035/1‑141 (MQ=255)
                                                       ccTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTa                                                                                     <  1:238812/141‑1 (MQ=255)
                                                           ttAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTgg                                                                                 <  1:190629/141‑1 (MQ=255)
                                                              acacTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACAAAAAAAAAAAAAAGTTCCGATAATTGTACTGGCTa                                                                              <  2:547114/141‑1 (MQ=255)
                                                               cacTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTAt                                                                             >  2:842302/1‑141 (MQ=255)
                                                                acTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATg                                                                            <  1:286749/141‑1 (MQ=255)
                                                                 cTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATgg                                                                           >  1:974518/1‑141 (MQ=255)
                                                                 cTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATgg                                                                           >  2:769778/1‑141 (MQ=255)
                                                                  tGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGt                                                                          >  1:234755/1‑141 (MQ=255)
                                                                    aGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTaa                                                                        <  2:388108/141‑1 (MQ=255)
                                                                      gCTAGGAAGATGTCTTTCGGTGGTAATGATAAAAAGTTCATCATTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATg                                                                      <  2:51254/141‑1 (MQ=255)
                                                                                tatCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCa                                                            <  1:225317/141‑1 (MQ=255)
                                                                                 atCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCat                                                           >  1:848635/1‑141 (MQ=255)
                                                                                   cTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCatat                                                         >  2:778981/1‑141 (MQ=255)
                                                                                      tCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGcc                                                      >  2:671616/1‑141 (MQ=255)
                                                                                       cGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCa                                                     <  1:834564/141‑1 (MQ=255)
                                                                                       cGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCa                                                     <  1:834547/141‑1 (MQ=255)
                                                                                       cGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCa                                                     <  1:707922/141‑1 (MQ=255)
                                                                                          tggtTATGTTAAAAAGATCATCGTTTCATATTTAATGCCTTTACCATCTGCCAAATATATGATCAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACAGGCTATGGTAATCAGTGTACGCATATGCCAGcc                                                  >  2:442230/1‑141 (MQ=255)
                                                                                             tAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGt                                               <  1:917399/141‑1 (MQ=255)
                                                                                                  aTAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGTCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGa                                          >  2:747508/1‑141 (MQ=255)
                                                                                                   tAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAg                                         <  2:208172/141‑1 (MQ=255)
                                                                                                   tAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAg                                         <  1:72398/141‑1 (MQ=255)
                                                                                                   tAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAg                                         <  2:253750/141‑1 (MQ=255)
                                                                                                     aaaaGTTCATCGTTTGATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCa                                       <  1:901084/141‑1 (MQ=255)
                                                                                                     aaaaGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCa                                       <  2:599932/141‑1 (MQ=255)
                                                                                                     aaaaGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCa                                       <  2:382736/141‑1 (MQ=255)
                                                                                                     aaaaGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACACAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCa                                       <  2:336290/141‑1 (MQ=255)
                                                                                                     aaaaGTTCATCGTTTCATATTTAATGCCTTTACCATCAGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCa                                       >  1:872688/1‑141 (MQ=255)
                                                                                                         gTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATaa                                   >  2:488134/1‑141 (MQ=255)
                                                                                                             aTCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTg                               >  1:877317/1‑141 (MQ=255)
                                                                                                               cGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAg                             >  1:226572/1‑141 (MQ=255)
                                                                                                                   tCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTAtt                         <  1:617882/141‑1 (MQ=255)
                                                                                                                      tatTTCTTGCCTGTATCTTCTTTCATATATATTATTTATTCTATTGATCTTTTACTCTAAAAATATCCGTTAACTTTACTTACTATGGTAATGAGTGTACGCATATGCCAGCCTGGACGTTGCAATAATGTGAGTTctgtt                      <  2:873815/141‑1 (MQ=255)
                                                                                                                            aTGCCTTTACCATCTGTCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAg                <  2:933968/141‑1 (MQ=255)
                                                                                                                            aTGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAg                >  1:883554/1‑141 (MQ=255)
                                                                                                                             tGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGa               <  1:107438/141‑1 (MQ=255)
                                                                                                                               ccTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGNACAGATc             >  2:811575/1‑141 (MQ=255)
                                                                                                                               ccTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATc             >  2:898475/1‑141 (MQ=255)
                                                                                                                               ccTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATACCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATc             >  1:827787/1‑141 (MQ=255)
                                                                                                                                cTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAGTTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCa            >  1:212041/1‑141 (MQ=255)
                                                                                                                                cTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCa            <  2:245645/141‑1 (MQ=255)
                                                                                                                                  ttACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATg          <  2:597737/141‑1 (MQ=255)
                                                                                                                                  ttACCATCTGACATATATATGATTAATTCTGTTAAAAAAAAAAAAAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATg          <  2:731668/141‑1 (MQ=255)
                                                                                                                                    aCCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTATTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATGGc        >  2:256145/1‑141 (MQ=255)
                                                                                                                                      cATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATGGCaa      <  2:306862/141‑1 (MQ=255)
                                                                                                                                       aTCTGTCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATGGCAAt     >  1:492172/1‑141 (MQ=255)
                                                                                                                                        tCTGCCATATATATGAATAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATGGCAATg    <  2:514380/141‑1 (MQ=255)
                                                                                                                                          tGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATGGCAATGGt  <  1:769778/141‑1 (MQ=255)
                                                                                                                                          tGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATGGCAATGGt  >  1:404979/1‑141 (MQ=255)
                                                                                                                                          tGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATGGCAATGGt  <  2:871534/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TTCATGCATACCACTAGCAATTGTAATTGCAACATCCATAATAGCGCCAAGTGAACCTATTAACACTGAGGCTAGGAAGATATCTTTCGGTGGTAATGATAAAAAGTTCATCGTTTCATATTTAATGCCTTTACCATCTGCCATATATATGATTAATTCTGTTAAACCTATACTCAAAAAAGTTCCGATAATTGTACTGGCTATGGTAATGAGTGTACGCATATGCCAGCCTGTAACGAGCAATAAAGTGAGTATTGTTGAACAGATCATGGCAATGGT  >  CP000730/495296‑495574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: