Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	584,277	C→T	5.8%	Y30Y (TAC→TAT)	rplJ →	ribosomal protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	584,277	0	C	T	5.8%	273.0 / 4.2	86	Y30Y (TAC→TAT)	rplJ	ribosomal protein L10
Reads supporting (aligned to +/- strand):  ref base C (37/44);  new base T (2/3);  total (39/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

TTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGCTTAGATGAATTCTTAAC  >  CP000730/584138‑584417                                                                                                                                            |                                                                                                                                             tttttATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTAcc                                                                                                                                             >  1:1013574/1‑141 (MQ=255)  ttttATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCg                                                                                                                                            <  1:460424/141‑1 (MQ=255)  ttttAATAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTAACATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCg                                                                                                                                            <  2:384972/141‑1 (MQ=255)    ttattaATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTg                                                                                                                                          <  1:1017138/141‑1 (MQ=255)       ttaATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGAt                                                                                                                                       <  2:999199/141‑1 (MQ=255)       ttaATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGAt                                                                                                                                       >  1:764056/1‑141 (MQ=255)       ttaATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGAt                                                                                                                                       <  1:692632/141‑1 (MQ=255)        taATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGAtt                                                                                                                                      <  2:1070392/141‑1 (MQ=255)         aaTTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTa                                                                                                                                     >  2:196139/1‑141 (MQ=255)           ttttAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAAc                                                                                                                                   <  2:718033/141‑1 (MQ=255)           ttttAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAAc                                                                                                                                   <  2:297481/141‑1 (MQ=255)           ttatAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCCTTGAAGCTAAAAAACAACTAGTTGATGAACTTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAAc                                                                                                                                   <  1:270468/138‑1 (MQ=255)            tttAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACa                                                                                                                                  <  1:244956/141‑1 (MQ=255)              tAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGt                                                                                                                                >  1:968427/1‑141 (MQ=255)              tAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGt                                                                                                                                <  2:960818/141‑1 (MQ=255)                aaaaaaGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTATCGTGGATTAACAGTAg                                                                                                                              >  1:64092/1‑141 (MQ=255)                aaaaaaGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAg                                                                                                                              >  1:408395/1‑141 (MQ=255)                aaaaaaGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATCCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAg                                                                                                                              >  1:921584/1‑141 (MQ=255)                 aaaaaGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACAGTGGATTAACAGTAGc                                                                                                                             <  1:440804/141‑1 (MQ=255)                  aaaaGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCt                                                                                                                            <  1:295119/141‑1 (MQ=255)                   aaaGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTg                                                                                                                           >  1:743950/1‑141 (MQ=255)                    aaGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGa                                                                                                                          <  2:188887/141‑1 (MQ=255)                     aGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGaa                                                                                                                         <  2:34104/141‑1 (MQ=255)                     aGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGaa                                                                                                                         >  1:560776/1‑141 (MQ=255)                      gCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAg                                                                                                                        >  1:398989/1‑141 (MQ=255)                       cACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGt                                                                                                                       >  1:710557/1‑141 (MQ=255)                          cAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTAc                                                                                                                    <  1:769404/141‑1 (MQ=255)                          cAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTAc                                                                                                                    >  1:353914/1‑141 (MQ=255)                           aaaaaTTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACt                                                                                                                   <  2:662327/141‑1 (MQ=255)                             aaaTTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGa                                                                                                                 <  2:204803/141‑1 (MQ=255)                               aTTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACt                                                                                                               <  1:124018/141‑1 (MQ=255)                                       ggAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTca                                                                                                       <  1:55395/141‑1 (MQ=255)                                         aGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTcaca                                                                                                     <  1:770189/141‑1 (MQ=255)                                         aGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTcaca                                                                                                     <  1:854549/141‑1 (MQ=255)                                         aGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTcaaa                                                                                                     >  2:157866/1‑139 (MQ=255)                                          ggTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACaa                                                                                                    <  1:33503/141‑1 (MQ=255)                                            tgtCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAAtt                                                                                                  <  1:1028131/141‑1 (MQ=255)                                             gtCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTATCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTa                                                                                                 <  1:784529/141‑1 (MQ=255)                                               cTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACg                                                                                               <  2:1003026/141‑1 (MQ=255)                                               cTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACg                                                                                               <  2:1003139/141‑1 (MQ=255)                                                   aTGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGaa                                                                                           <  1:1065316/141‑1 (MQ=255)                                                     gTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTATCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGc                                                                                         <  1:539053/141‑1 (MQ=255)                                                     gTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGc                                                                                         <  1:169292/141‑1 (MQ=255)                                                     gTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGc                                                                                         <  2:417165/141‑1 (MQ=255)                                                       cTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTg                                                                                       <  2:243652/141‑1 (MQ=255)                                                         gCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGgt                                                                                     >  2:357545/1‑141 (MQ=255)                                                          cTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGgtg                                                                                    >  1:618508/1‑141 (MQ=255)                                                          cTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGgtg                                                                                    >  2:151883/1‑141 (MQ=255)                                                           tATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGgtgt                                                                                   <  2:491180/141‑1 (MQ=255)                                                            aTCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGtt                                                                                  <  1:623515/141‑1 (MQ=255)                                                                 tGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGta                                                                             >  2:616618/1‑141 (MQ=255)                                                                   aaGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGCGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGtata                                                                           >  2:637044/1‑141 (MQ=255)                                                                    aGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATaa                                                                          >  2:704685/1‑141 (MQ=255)                                                                     gCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTATCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATaaa                                                                         >  1:189446/1‑141 (MQ=255)                                                                       tAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGt                                                                       <  2:220228/141‑1 (MQ=255)                                                                              caacTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACaaa                                                                <  1:717516/141‑1 (MQ=255)                                                                               aacTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACaaaa                                                               <  2:239252/141‑1 (MQ=255)                                                                                acTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACaaaaa                                                              >  1:59685/1‑141 (MQ=255)                                                                                acTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACaaaaa                                                              >  2:459068/1‑141 (MQ=255)                                                                                acTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACaaaaa                                                              >  2:1060253/1‑141 (MQ=255)                                                                                acTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACaaaaa                                                              >  1:934926/1‑141 (MQ=255)                                                                                acTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACaaaaa                                                              >  1:811767/1‑141 (MQ=255)                                                                                 cTAGTTGATGGAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAac                                                             <  2:927744/141‑1 (MQ=255)                                                                                  tAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAaca                                                            >  1:865172/1‑141 (MQ=255)                                                                                           aaaTTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTAc                                                   <  1:1075454/141‑1 (MQ=255)                                                                                             aTTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTAcgt                                                 >  2:999792/1‑141 (MQ=255)                                                                                              ttGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTCATGACTTAACTCCACACACAAACGAAGATCGCGTAGAGCATCAAGTATACAAAAACACTATGGTAcgtc                                                >  1:99049/1‑141 (MQ=255)                                                                                                gCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTAcgtcgt                                              >  1:162504/1‑141 (MQ=255)                                                                                                  tGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTATCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGc                                            <  2:64092/141‑1 (MQ=255)                                                                                                     ggTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGc                                         >  1:773684/1‑141 (MQ=255)                                                                                                       tACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTg                                       <  2:932302/141‑1 (MQ=255)                                                                                                            tCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGCATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGaaaaa                                  >  2:652826/1‑141 (MQ=255)                                                                                                             cAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAg                                 >  1:97855/1‑141 (MQ=255)                                                                                                              aaaTTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTCCGTCGTGCAGCTGAAAAAGc                                >  1:227710/1‑141 (MQ=255)                                                                                                                 ttCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTgg                             <  1:641399/141‑1 (MQ=255)                                                                                                                   cAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGGAAAAGCTGGTa                           >  2:723712/1‑141 (MQ=255)                                                                                                                     gTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATc                         >  1:452491/1‑141 (MQ=255)                                                                                                                       ttCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAACACTCTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGa                       <  2:926323/141‑1 (MQ=255)                                                                                                                       ttCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGa                       <  2:16246/141‑1 (MQ=255)                                                                                                                           aCAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGc                   >  1:657814/1‑141 (MQ=255)                                                                                                                           aCAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGc                   <  1:57213/141‑1 (MQ=255)                                                                                                                               tAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGCTTAg               >  1:91472/1‑141 (MQ=255)                                                                                                                                aaTCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACTTGAATGTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGCTTAGa              <  1:811025/141‑1 (MQ=255)                                                                                                                                 aTCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGCTTAGAt             >  1:263211/1‑141 (MQ=255)                                                                                                                                  tCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGCTTAGATg            <  1:707171/141‑1 (MQ=255)                                                                                                                                  tCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGCTTAGATg            >  2:404722/1‑141 (MQ=255)                                                                                                                                   cGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGCTTAGATGa           >  1:551972/1‑141 (MQ=255)                                                                                                                                            ccGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGCTTAGATGAATTCTTAAc  <  1:1064378/141‑1 (MQ=255)                                                                                                                                            ccGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGCTTAGATGAATTCTTAAc  <  1:221060/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                             TTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCAAATTCAGTTTCAACAGTAATCGTTGACTACCGTGGATTAACAGTAGCTGAAGTTACTGACTTACGTTCACAATTACGTGAAGCTGGTGTTGAGTATAAAGTATACAAAAACACTATGGTACGTCGTGCAGCTGAAAAAGCTGGTATCGAAGGCTTAGATGAATTCTTAAC  >  CP000730/584138‑584417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑