BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	631,293	T→A	5.2%	G189G (GGT→GGA)	USA300HOU_0566 →	possible phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	631,293	0	T	A	5.2%	331.6 / 6.6	110	G189G (GGT→GGA)	USA300HOU_0566	possible phosphatase
Reads supporting (aligned to +/- strand): ref base T (54/48); new base A (2/4); total (56/54)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 111 total aligned reads displayed.

AAACCAAATCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCATAAATATTAGATTAT  >  CP000730/631153‑631432

aaaCCAAATCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGt                                                                                                                                             <  1:17382/141‑1 (MQ=255)

   ccAAATCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGAAATCTGCAGCTGTCACTTGGGGTGca                                                                                                                                          >  2:860156/1‑141 (MQ=255)

    cAAATCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGcac                                                                                                                                         <  2:186505/141‑1 (MQ=255)

     aaaTCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGcaca                                                                                                                                        >  2:1045712/1‑141 (MQ=255)

      aaTCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACat                                                                                                                                       >  2:625347/1‑141 (MQ=255)

      aaTCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACat                                                                                                                                       >  2:128936/1‑141 (MQ=255)

       aTCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACatg                                                                                                                                      >  1:682484/1‑141 (MQ=255)

            gAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGCTTCAACGTTTGCTGTTGGGCTGGCACAACGTGATGGTCTGCAATCTGCAGGTGTCCCTTGGGGTGCCCatgatgaa                                                                                                                                 >  1:864373/1‑139 (MQ=255)

            gAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCa                                                                                                                                 >  2:131833/1‑141 (MQ=255)

            gAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCa                                                                                                                                 >  2:30888/1‑141 (MQ=255)

               ggCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAgg                                                                                                                              >  2:251532/1‑141 (MQ=255)

               ggCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAgg                                                                                                                              >  1:674297/1‑141 (MQ=255)

               ggCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAgg                                                                                                                              <  2:771039/141‑1 (MQ=255)

                 cATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGTAAGGTc                                                                                                                            >  1:381974/1‑141 (MQ=255)

                 aaTACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTc                                                                                                                            <  2:394633/140‑1 (MQ=255)

                  aTACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCa                                                                                                                           <  1:171644/141‑1 (MQ=255)

                    acacacAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCAtt                                                                                                                         <  1:87751/141‑1 (MQ=255)

                    acacacAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGGCAtt                                                                                                                         >  2:616979/1‑141 (MQ=255)

                       cacaATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACt                                                                                                                      <  2:249737/141‑1 (MQ=255)

                             tgtgCAACGCTACACTTTAAATAGCCAACAAACGGTGTATATTGGTGATTAAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACttcattc                                                                                                                >  2:788261/1‑141 (MQ=255)

                                gCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCaaa                                                                                                             >  1:866929/1‑141 (MQ=255)

                                gCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCaaa                                                                                                             >  1:954708/1‑141 (MQ=255)

                                  aaCGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATc                                                                                                           <  1:1046939/141‑1 (MQ=255)

                                  aaCGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATc                                                                                                           <  2:402386/141‑1 (MQ=255)

                                   aCGCTACAATTTAAATAGCCAACAAACGGTTTATATTGGTGATTCAACATTTGATGTTGAGATGGCGCAACATGCTGGTGTACCATCTGCAGCAGTAACATGGGGAGCACATGATGCAAGGTCATTACTTCATTCAAATcc                                                                                                          >  2:174183/1‑141 (MQ=255)

                                   aCGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATcc                                                                                                          <  1:554298/141‑1 (MQ=255)

                                   aCGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATcc                                                                                                          >  1:134433/1‑141 (MQ=255)

                                   aCGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATcc                                                                                                          >  2:441144/1‑141 (MQ=255)

                                   aCGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGCGCACATGATGCAAGGTCATTACTTCATTCAAATcc                                                                                                          <  1:122041/141‑1 (MQ=255)

                                   aCGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGCATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATcc                                                                                                          >  2:575399/1‑141 (MQ=255)

                                     gCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCgg                                                                                                        <  1:478368/141‑1 (MQ=255)

                                         cAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGAttt                                                                                                    <  1:261167/141‑1 (MQ=255)

                                         cAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGAttt                                                                                                    <  1:261157/141‑1 (MQ=255)

                                          aaTTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGAtttt                                                                                                   <  2:336521/141‑1 (MQ=255)

                                             ttAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTttatt                                                                                                >  2:91217/1‑141 (MQ=255)

                                                aaTAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTttattatt                                                                                             <  1:305317/141‑1 (MQ=255)

                                                 aTAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTttattatta                                                                                            <  1:991088/141‑1 (MQ=255)

                                                 aTAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTttattatta                                                                                            <  1:687046/141‑1 (MQ=255)

                                                    gCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATg                                                                                         >  2:402973/1‑141 (MQ=255)

                                                            aCGGTGTATATTGGTGATTCAACGCTTGATGTTTAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCa                                                                                 >  1:657985/1‑141 (MQ=255)

                                                              ggTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGa                                                                               >  2:172193/1‑141 (MQ=255)

                                                              ggTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGAACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGa                                                                               >  1:333611/1‑141 (MQ=255)

                                                                tgtATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGaaa                                                                             <  1:697326/141‑1 (MQ=255)

                                                                  tataTTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAAtt                                                                           <  1:557105/141‑1 (MQ=255)

                                                                    tatTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTaa                                                                         <  2:171511/141‑1 (MQ=255)

                                                                    tatTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTaa                                                                         <  2:414286/141‑1 (MQ=255)

                                                                     atTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAAt                                                                        <  2:2842/141‑1 (MQ=255)

                                                                     atTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAAt                                                                        <  2:42776/141‑1 (MQ=255)

                                                                     atTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAAt                                                                        <  1:111193/141‑1 (MQ=255)

                                                                     atTGGTGATTCAACATTTGATGTTGAGATGGCGCAACATGCTGGTGTACCATCTGCAGCAGTAACATGGGGAGCACATGATGCAAGGTCATTACTTCATTCAAATCCAGATTTTATAATCAATGATCCATCAGAAATTAAt                                                                        >  1:323824/1‑141 (MQ=255)

                                                                               cAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGtattat                                                              >  2:728836/1‑141 (MQ=255)

                                                                               cAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGtattat                                                              >  1:824638/1‑141 (MQ=255)

                                                                                aaCGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATtata                                                             >  2:425321/1‑141 (MQ=255)

                                                                                 aCGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATaa                                                            >  1:843424/1‑141 (MQ=255)

                                                                                  cGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATaaa                                                           >  1:905878/1‑141 (MQ=255)

                                                                                   gTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATaaaa                                                          >  2:872186/1‑141 (MQ=255)

                                                                                    tttGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAAc                                                         <  1:617604/141‑1 (MQ=255)

                                                                                     ttGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACt                                                        >  2:674651/1‑141 (MQ=255)

                                                                                     caaacgTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGCCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACt                                                        >  1:976686/6‑141 (MQ=255)

                                                                                       gATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTg                                                      >  1:520968/1‑141 (MQ=255)

                                                                                       gATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTg                                                      >  1:670069/1‑141 (MQ=255)

                                                                                       gATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTg                                                      >  1:670225/1‑141 (MQ=255)

                                                                                        aTGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGt                                                     >  1:384944/1‑141 (MQ=255)

                                                                                        aTGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGt                                                     <  1:328668/141‑1 (MQ=255)

                                                                                        aTGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGt                                                     <  2:1080832/141‑1 (MQ=255)

                                                                                        aTGTTGAGAGGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGt                                                     <  2:298501/141‑1 (MQ=255)

                                                                                         tGTTGAGATGGCGCAACATGCTGGTGTACCATCTGCAGCAGTAACATGGGGAGCACATGATGCAAGGTCATTACTTCATTCAAATCCAGATTTTATAATCAATGATCCATCAGAAATTAATACAGTATTATAAAATTTGtt                                                    <  1:174183/141‑1 (MQ=255)

                                                                                         tGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGtt                                                    <  1:625347/141‑1 (MQ=255)

                                                                                         tGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGtt                                                    >  1:353849/1‑141 (MQ=255)

                                                                                          gTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAGTGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTa                                                   <  2:215115/141‑1 (MQ=255)

                                                                                              agaTGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAAc                                               >  1:766330/1‑141 (MQ=255)

                                                                                                   gCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAgaga                                          <  1:674188/141‑1 (MQ=255)

                                                                                                   gCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAgaga                                          >  1:397712/1‑141 (MQ=255)

                                                                                                   gCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAgaga                                          >  1:1058805/1‑141 (MQ=255)

                                                                                                    cacaACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGaa                                         <  2:611691/141‑1 (MQ=255)

                                                                                                    cacaACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGaa                                         <  2:641544/141‑1 (MQ=255)

                                                                                                       aaCGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATAc                                      >  2:1069825/1‑141 (MQ=255)

                                                                                                           tGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATg                                  <  2:291902/141‑1 (MQ=255)

                                                                                                            gCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATgg                                 <  2:801199/141‑1 (MQ=255)

                                                                                                            gCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATgg                                 <  2:64944/141‑1 (MQ=255)

                                                                                                             cTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGt                                >  2:293750/1‑141 (MQ=255)

                                                                                                             cTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGt                                <  1:179614/141‑1 (MQ=255)

                                                                                                              tGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGtt                               >  1:76596/1‑141 (MQ=255)

                                                                                                              tGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGtt                               <  2:342690/141‑1 (MQ=255)

                                                                                                                   tGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAAt                          <  2:884267/141‑1 (MQ=255)

                                                                                                                      aaTCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCa                       <  1:251532/141‑1 (MQ=255)

                                                                                                                       aTCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCat                      >  2:708788/1‑141 (MQ=255)

                                                                                                                        tCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCata                     >  2:528400/1‑141 (MQ=255)

                                                                                                                          tGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATAtt                   <  1:250412/141‑1 (MQ=255)

                                                                                                                            cagcagtaacatGGGGAGCACATGATGCAAGGTCATTACTTCATTCAAATCCAGATTTTATAATCAATGATCCATCAGAAATTAATACAGTATTATAAAATTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCa                 <  1:772437/130‑1 (MQ=255)

                                                                                                                            cAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCa                 <  1:578993/141‑1 (MQ=255)

                                                                                                                              gCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCATa               <  1:180574/141‑1 (MQ=255)

                                                                                                                              gCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCATa               >  2:310303/1‑141 (MQ=255)

                                                                                                                              gCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCATa               >  2:310371/1‑141 (MQ=255)

                                                                                                                               cTGTCACTTGGGGTGCACATGATGCAAGGTCATTAGTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCATaa              >  1:948154/1‑141 (MQ=255)

                                                                                                                                  taacatGGGGAGCACATGATGCAAGGTCATTACTTCATTCAAATCCAGATTTTATAATCAATGATCCATCAGAAATTAATACAGTATTATAAAATTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCATAAata           <  2:250171/136‑1 (MQ=255)

                                                                                                                                   cACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCATAAatat          >  2:227621/1‑141 (MQ=255)

                                                                                                                                   cACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCACGGTTAAAATGCATATTCATAAatat          >  2:485644/1‑141 (MQ=255)

                                                                                                                                     cTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCATAAATATTa        <  2:693359/141‑1 (MQ=255)

                                                                                                                                           gTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCATAAATATTAGATtat  <  2:725043/141‑1 (MQ=255)

AAACCAAATCCTGAAGGCATACACACAATTGTGCAACGCTACAATTTAAATAGCCAACAAACGGTGTATATTGGTGATTCAACGTTTGATGTTGAGATGGCACAACGTGCTGGTATGCAATCTGCAGCTGTCACTTGGGGTGCACATGATGCAAGGTCATTACTTCATTCAAATCCGGATTTTATTATTAATGATCCATCAGAAATTAATACCGTATTATAAAACTTGTTAAAACAGAGAATACCATGGTTAAAATGCATATTCATAAATATTAGATTAT  >  CP000730/631153‑631432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`