Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 696,932 T→A 5.4% G300G (GGT→GGA mnhA1 → CPA3 family monovalent cation (K+ or Na+):proton (H+) antiporter‑3, MnhA subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730696,9320TA 5.4% 261.4 / 4.3 86G300G (GGT→GGAmnhA1CPA3 family monovalent cation (K+ or Na+):proton (H+) antiporter‑3, MnhA subunit
Reads supporting (aligned to +/- strand):  ref base T (40/41);  new base A (3/2);  total (43/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAAT  >  CP000730/696792‑697072
                                                                                                                                            |                                                                                                                                            
aaaGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGt                                                                                                                                              >  1:269464/1‑141 (MQ=255)
aaaGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGt                                                                                                                                              >  1:1061126/1‑141 (MQ=255)
   gCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATc                                                                                                                                           <  1:503767/141‑1 (MQ=255)
     tGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCt                                                                                                                                         >  2:680981/1‑141 (MQ=255)
       gTATCTTTTTATTATTTTTATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAg                                                                                                                                       >  2:191034/1‑141 (MQ=255)
       gTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAg                                                                                                                                       >  2:641007/1‑141 (MQ=255)
        tATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGc                                                                                                                                      <  1:477966/141‑1 (MQ=255)
          tCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGt                                                                                                                                    <  2:961719/141‑1 (MQ=255)
             ttttATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTAct                                                                                                                                 <  1:52670/141‑1 (MQ=255)
              tttATTACTTCTATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTActc                                                                                                                                >  2:596732/1‑141 (MQ=255)
                 attaCTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATTACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTAc                                                                                                                             >  1:1029732/1‑141 (MQ=255)
                       tCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAg                                                                                                                       <  1:841944/141‑1 (MQ=255)
                        cGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGt                                                                                                                      <  1:413776/141‑1 (MQ=255)
                         gATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTc                                                                                                                     <  1:339341/141‑1 (MQ=255)
                             tACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACt                                                                                                                 >  2:483351/1‑141 (MQ=255)
                               cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                               >  2:718277/1‑141 (MQ=255)
                               cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                               >  2:499583/1‑141 (MQ=255)
                               cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                               >  1:850539/1‑141 (MQ=255)
                               cacCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTg                                                                                                               >  2:763660/1‑141 (MQ=255)
                                  cATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGga                                                                                                            <  1:166746/141‑1 (MQ=255)
                                   attattAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGgat                                                                                                           >  2:477830/1‑141 (MQ=255)
                                     tattaGGTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGaatga                                                                                                         <  2:50171/141‑1 (MQ=255)
                                         aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                                                                                     <  1:87939/141‑1 (MQ=255)
                                         aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                                                                                     >  1:1046372/1‑141 (MQ=255)
                                         aGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTAt                                                                                                     >  1:1046528/1‑141 (MQ=255)
                                           gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                                                                                                   >  1:526260/1‑141 (MQ=255)
                                           gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                                                                                                   >  1:212725/1‑141 (MQ=255)
                                           gTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATgg                                                                                                   >  2:406734/1‑141 (MQ=255)
                                           gTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATgg                                                                                                   <  1:472694/141‑1 (MQ=255)
                                           gTCTTAGCAATATGTACATATATATCGTCACGTTTGTTGGTTTAATAACAAAGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATgg                                                                                                   >  2:409041/1‑141 (MQ=255)
                                                  cAATATGTACGTATATATCGTTACGCTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGCTTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTAtggt                                                                                            >  2:949593/1‑141 (MQ=255)
                                                   aaTATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATAACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTAtggta                                                                                           <  1:1028510/141‑2 (MQ=255)
                                                       tGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATTAGTCAACTTGGGATGATTATGGCTATGGTGGGta                                                                                       >  1:534463/1‑141 (MQ=255)
                                                       tGTACATATATATCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTCAACTAGGAATGATTATGGCAATGGTCGGta                                                                                       >  2:790719/1‑141 (MQ=255)
                                                         tACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGtata                                                                                     >  1:366526/1‑141 (MQ=255)
                                                          aCGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAg                                                                                    <  2:792135/141‑1 (MQ=255)
                                                          aCGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAg                                                                                    <  2:688604/141‑1 (MQ=255)
                                                            gTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGt                                                                                  >  2:66758/1‑141 (MQ=255)
                                                             tatataTCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTg                                                                                 <  2:269464/141‑1 (MQ=255)
                                                              atatatCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTgg                                                                                <  2:850633/141‑1 (MQ=255)
                                                               tatatCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAAGAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGc                                                                               <  1:661922/141‑1 (MQ=255)
                                                                  ttCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa                                                                            <  1:891516/140‑1 (MQ=255)
                                                                  atCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa                                                                            <  1:183151/141‑1 (MQ=255)
                                                                  atCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGa                                                                            <  1:943320/141‑1 (MQ=255)
                                                                   tCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGat                                                                           <  2:240271/141‑1 (MQ=255)
                                                                   tCGTCACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATCACAGCTTTAAAACAATGGGATTTAAAAGGAATCCTAGCATATTCAACAATCAGTAAACTAGGAATGATTATGGCAATGGTCGGTATCGGTGGTGGat                                                                           >  1:204624/1‑141 (MQ=255)
                                                                    cGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGata                                                                          <  2:1040827/141‑1 (MQ=255)
                                                                     gTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGatat                                                                         <  2:453683/141‑1 (MQ=255)
                                                                     gTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGatat                                                                         >  1:406326/1‑141 (MQ=255)
                                                                      ttACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATg                                                                        <  2:302176/141‑1 (MQ=255)
                                                                       tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc                                                                       >  1:661539/1‑141 (MQ=255)
                                                                       tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc                                                                       >  1:97988/1‑141 (MQ=255)
                                                                       tACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGc                                                                       >  2:400823/1‑141 (MQ=255)
                                                                          gTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCa                                                                    >  1:993416/1‑141 (MQ=255)
                                                                           tttGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGATTTATAAAATTGGGATTTAATTGATGACTTAGTCTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCaa                                                                   <  1:647594/141‑1 (MQ=255)
                                                                           taattgaaccaaataaCATTGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCaa                                                                   >  2:378934/12‑141 (MQ=255)
                                                                            ttgttgGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAac                                                                  >  1:404146/1‑141 (MQ=255)
                                                                             tgttgGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAaca                                                                 <  1:234458/141‑1 (MQ=255)
                                                                               ttgGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACAcc                                                               <  1:596732/141‑1 (MQ=255)
                                                                                    ttAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACcaacaa                                                          <  1:972508/141‑1 (MQ=255)
                                                                                     taataaCAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAg                                                         >  1:688480/1‑141 (MQ=255)
                                                                                         aaCAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGc                                                     >  1:537701/1‑141 (MQ=255)
                                                                                            aaTGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAAt                                                  <  2:290607/141‑1 (MQ=255)
                                                                                               gTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGc                                               <  2:163246/141‑1 (MQ=255)
                                                                                                  aTTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATc                                            >  1:46561/1‑141 (MQ=255)
                                                                                                      ggTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTAtt                                        <  1:842925/141‑1 (MQ=255)
                                                                                                       gTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTAttt                                       >  2:880435/1‑141 (MQ=255)
                                                                                                         tCAATTCCAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTAATCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAt                                     <  1:867402/141‑1 (MQ=255)
                                                                                                         tCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAt                                     <  1:1084320/141‑1 (MQ=255)
                                                                                                          caaatACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtg                                    <  2:285899/137‑1 (MQ=255)
                                                                                                          cAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtg                                    >  2:804408/1‑141 (MQ=255)
                                                                                                            aTTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtgta                                  >  2:245824/1‑141 (MQ=255)
                                                                                                                  gCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTAtgtatttgta                            >  1:705877/1‑141 (MQ=255)
                                                                                                                      tAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGtattat                        >  1:406223/1‑141 (MQ=255)
                                                                                                                              tGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcg                <  2:86087/141‑1 (MQ=255)
                                                                                                                              tGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcg                <  1:1075130/141‑1 (MQ=255)
                                                                                                                               gggATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcgc               <  2:177707/141‑1 (MQ=255)
                                                                                                                               gggATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcgc               <  1:628697/141‑1 (MQ=255)
                                                                                                                               gggATTTAAAAGGTAGCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACGCCAACATGGCGCAATAGCATCTATTTATGTATTTGTATTATTTGGTgcgc               <  2:209791/141‑1 (MQ=255)
                                                                                                                                ggATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCt              <  2:32194/141‑1 (MQ=255)
                                                                                                                                 gATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTa             >  1:269434/1‑141 (MQ=255)
                                                                                                                                  aTTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTAt            >  2:234547/1‑141 (MQ=255)
                                                                                                                                  aTTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTAt            >  1:347279/1‑141 (MQ=255)
                                                                                                                                     tAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTc         <  1:378934/141‑1 (MQ=255)
                                                                                                                                       aaaGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCAt       <  2:178637/141‑1 (MQ=255)
                                                                                                                                          ggTATCCTAGCGTACTCTACAATCAGTCAACTTGGCATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTa    >  1:948642/1‑141 (MQ=255)
                                                                                                                                           gTATCCTAGCGTACTCTACAATCAGTCAACTTTGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTaa   <  1:60361/141‑1 (MQ=255)
                                                                                                                                           gTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTaa   >  1:626406/1‑141 (MQ=255)
                                                                                                                                            tATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAAt  <  2:491616/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAAGCTGGTATCTTTTTATTACTTCGATTTACACCATTATTAGGTCTTAGCAATATGTACGTATATATCGTTACGTTTGTTGGTTTAATAACAATGTTATTTGGTTCAATTACAGCTTTAAAACAATGGGATTTAAAAGGTATCCTAGCGTACTCTACAATCAGTCAACTTGGGATGATTATGGCTATGGTGGGTATAGGTGGCGGATATGCTCAACACCAACAAGACGCAATAGCATCTATTTATGTATTTGTATTATTTGGTGCGCTATTTCATCTAAT  >  CP000730/696792‑697072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: