Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 748,209 T→C 6.3% A68A (GCT→GCC USA300HOU_0694 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730748,2090TC 6.3% 338.5 / 9.0 123A68A (GCT→GCCUSA300HOU_0694LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (56/59);  new base C (4/4);  total (60/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 124 total aligned reads displayed.

CTGAAATTTTATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCTATATAATGC  >  CP000730/748070‑748349
                                                                                                                                           |                                                                                                                                            
cTGAAATTTTATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATAAGAAAAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTc                                                                                                                                             <  2:311594/141‑1 (MQ=255)
   aaaTTTTATATAAATCTCAACCTGGTGTTACACAAAGACTAAAAGGAATTGAAAATGC‑‑TTGGAGTAGATATTTATATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCCCGTGAc                                                                                                                                        <  2:560234/141‑1 (MQ=255)
    caTTTTATATGTATCACAACATTCTGTAACACAAAGACTTAAAGCCATTGAAAATGCTTCTCGAGTAGATATGTATAAAAAAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGa                                                                                                                                         <  2:1034159/140‑1 (MQ=255)
        ttATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAAAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATg                                                                                                                                     <  2:910638/141‑1 (MQ=255)
            atatatCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCCCGTGACATGTTGa                                                                                                                                 >  2:498971/1‑141 (MQ=255)
            atatatCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTAATAAAAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGa                                                                                                                                 <  2:367225/141‑1 (MQ=255)
              atatCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGaaa                                                                                                                               <  2:195948/141‑1 (MQ=255)
                       ccTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTTTTATAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCCCGTGACATGTTGAAAAGAGAGCGa                                                                                                                      >  2:477030/1‑141 (MQ=255)
                       ccTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACATCTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGa                                                                                                                      <  1:1003235/141‑1 (MQ=255)
                       ccTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGa                                                                                                                      <  1:223823/141‑1 (MQ=255)
                       ccTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCCCGTGACATGTTGAAAAGAGAGCGa                                                                                                                      >  2:536022/1‑141 (MQ=255)
                         tgctgTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGatt                                                                                                                    >  2:343026/1‑141 (MQ=255)
                          gctgTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGatta                                                                                                                   >  1:354037/1‑141 (MQ=255)
                          gctgTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGatta                                                                                                                   >  2:829052/1‑141 (MQ=255)
                            tgTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATTATTATTTAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTTAAAAGAGAGCGattatt                                                                                                                 >  2:1738/1‑141 (MQ=255)
                             gTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTAttt                                                                                                                >  2:838305/1‑141 (MQ=255)
                             gTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTAttt                                                                                                                >  2:303039/1‑141 (MQ=255)
                                  acaAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGa                                                                                                           <  2:1029848/141‑1 (MQ=255)
                                  acaAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGa                                                                                                           <  2:321021/141‑1 (MQ=255)
                                  acaAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGa                                                                                                           <  1:430912/141‑1 (MQ=255)
                                   caAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGAc                                                                                                          <  1:645944/141‑1 (MQ=255)
                                       gACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACaaaa                                                                                                      >  1:617344/1‑141 (MQ=255)
                                           aaaaGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCa                                                                                                  >  1:504348/1‑141 (MQ=255)
                                               gCTATTGAAAATGCTTTGGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGca                                                                                              >  2:163431/1‑141 (MQ=255)
                                                cattttcaatagCTTTTAGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGATCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAACTGCATGcac                                                                                             >  2:291019/12‑141 (MQ=255)
                                                  tcttcaatagCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACat                                                                                           >  1:431348/10‑141 (MQ=255)
                                                  aTTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACat                                                                                           <  1:643565/141‑1 (MQ=255)
                                                  aTTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACat                                                                                           <  1:871341/141‑1 (MQ=255)
                                                   tttcaatagCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACata                                                                                          >  1:381023/9‑141 (MQ=255)
                                                   tttcaatagCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACata                                                                                          >  1:55977/9‑141 (MQ=255)
                                                   ttGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACata                                                                                          >  1:625087/1‑141 (MQ=255)
                                                     gAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATCtt                                                                                        >  2:45563/1‑141 (MQ=255)
                                                     gAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGAATGCCCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATAtt                                                                                        >  1:597602/1‑141 (MQ=255)
                                                     gAAAATGCATTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTCTTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTCTTTGATAAAATGAAGGCCCATAtt                                                                                        >  2:50792/1‑141 (MQ=255)
                                                      caatagCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTg                                                                                       >  1:494746/6‑141 (MQ=255)
                                                      aaaaTGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTTACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTg                                                                                       >  1:46029/1‑141 (MQ=255)
                                                         aTGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtg                                                                                    <  1:1052772/141‑1 (MQ=255)
                                                          tGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtga                                                                                   >  1:733950/1‑141 (MQ=255)
                                                           gCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtgaa                                                                                  >  1:454606/1‑141 (MQ=255)
                                                            cTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGAAGACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgttaag                                                                                 <  1:576222/141‑1 (MQ=255)
                                                            cTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTCATTACAAAAACTGAAGAAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtgaag                                                                                 <  1:712613/141‑1 (MQ=255)
                                                               ttGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtgaagtga                                                                              >  1:87950/1‑141 (MQ=255)
                                                               ttGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtgaagtga                                                                              >  1:228607/1‑141 (MQ=255)
                                                               ttGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtgaagtga                                                                              >  2:831728/1‑141 (MQ=255)
                                                                tGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGCAATGGTGATAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtgaagtgaa                                                                             >  1:613675/1‑141 (MQ=255)
                                                                      aGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAAc                                                                       >  1:654090/1‑141 (MQ=255)
                                                                      aGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAAc                                                                       >  2:331010/1‑141 (MQ=255)
                                                                      aGATATTTTTATAAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAAc                                                                       <  2:430493/141‑1 (MQ=255)
                                                                       gATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACa                                                                      >  1:796778/1‑141 (MQ=255)
                                                                         tatTTTTATAAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAat                                                                    <  2:278481/141‑1 (MQ=255)
                                                                          atTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAata                                                                   >  1:679292/1‑141 (MQ=255)
                                                                           tttttATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAatat                                                                  <  2:183245/141‑1 (MQ=255)
                                                                              ttttctgtaCAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCaa                                                               >  2:247278/9‑141 (MQ=255)
                                                                              ttATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCaa                                                               >  1:885699/1‑141 (MQ=255)
                                                                               tATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAAt                                                              <  1:182773/141‑1 (MQ=255)
                                                                                   aGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCggg                                                          >  1:847481/1‑141 (MQ=255)
                                                                                    gAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGgt                                                         <  1:797995/141‑1 (MQ=255)
                                                                                      aCAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTTTTTTTTTTCTGGCACAGAAAGGGGAAGTGAATTGAACAATATCAAGCgtgggg                                                       >  2:1083695/1‑136 (MQ=255)
                                                                                       cAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGtt                                                      <  1:246288/141‑1 (MQ=255)
                                                                                         aaaaaaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGttct                                                    <  1:786965/141‑1 (MQ=255)
                                                                                         aaaaaaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGttct                                                    <  2:574219/141‑1 (MQ=255)
                                                                                            aaaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTtcttct                                                 <  1:714919/141‑1 (MQ=255)
                                                                                            aaaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTtcttct                                                 <  1:601972/141‑1 (MQ=255)
                                                                                             aaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTcttctt                                                >  1:321618/1‑141 (MQ=255)
                                                                                             aaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTcttctt                                                >  1:937679/1‑141 (MQ=255)
                                                                                              aCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCttt                                               >  1:890997/1‑141 (MQ=255)
                                                                                              aCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCttt                                               >  1:557015/1‑141 (MQ=255)
                                                                                                aaTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGa                                             <  1:645706/141‑1 (MQ=255)
                                                                                                aaTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGa                                             <  1:617635/141‑1 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            >  1:993415/1‑141 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            >  2:321642/1‑141 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            >  2:784277/1‑141 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            <  2:315101/141‑1 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            <  2:838837/141‑1 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            >  1:125334/1‑141 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            <  1:1019760/141‑1 (MQ=255)
                                                                                                   tGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTg                                          >  2:21391/1‑141 (MQ=255)
                                                                                                    gATTACAACAACTGAAGGAACAATGATTATTGAGCATGCCCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATGGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTgg                                         <  1:875580/141‑1 (MQ=255)
                                                                                                           acaacTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAAcc                                  >  1:566794/1‑141 (MQ=255)
                                                                                                           acaacTGAAGGAACAATGATTAGTGAGCATGCCCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAAcc                                  <  1:638217/141‑1 (MQ=255)
                                                                                                                  aaGGAACAAGGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTc                           <  2:254756/141‑1 (MQ=255)
                                                                                                                   aGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTcc                          <  1:829052/141‑1 (MQ=255)
                                                                                                                    ggAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCt                         >  2:902793/1‑141 (MQ=255)
                                                                                                                       aCAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGaa                      <  2:890495/141‑1 (MQ=255)
                                                                                                                         aaTGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCCATCGGGTGCTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGt                    >  2:747736/1‑141 (MQ=255)
                                                                                                                          aTGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGtt                   <  1:181779/141‑1 (MQ=255)
                                                                                                                           tGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGttt                  <  1:1058731/141‑1 (MQ=255)
                                                                                                                           tGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGttt                  <  1:304420/141‑1 (MQ=255)
                                                                                                                           tGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGttt                  <  1:765868/141‑1 (MQ=255)
                                                                                                                            gATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATc                                                                 >  2:877914/1‑93 (MQ=255)
                                                                                                                              ttattGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGa               >  1:655430/1‑141 (MQ=255)
                                                                                                                               tattGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACACACCTTACTTCCTGAAGTTTTAAg              >  2:439699/1‑141 (MQ=255)
                                                                                                                               tattGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAg              <  2:288493/141‑1 (MQ=255)
                                                                                                                                attGAGAATGCTCGAGACATGTTGAAAAGACAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGc             <  2:43160/141‑1 (MQ=255)
                                                                                                                                 ttGAGCATGCTCGTGACATGTTGAAAAGAGGGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGcc            <  2:63339/141‑1 (MQ=255)
                                                                                                                                 ttGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTTTCTTTTTGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGcc            >  2:265434/1‑141 (MQ=255)
                                                                                                                                 ttGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGcc            >  1:627926/1‑141 (MQ=255)
                                                                                                                                  tGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCt           <  2:132217/141‑1 (MQ=255)
                                                                                                                                         gCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCTATATAAt    <  2:61496/141‑1 (MQ=255)
                                                                                                                                           tCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCTATATAATGc  <  1:311372/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CTGAAATTTTATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCTATATAATGC  >  CP000730/748070‑748349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: