Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 795,968 C→T 6.3% R296R (CGC→CGT recQ1 → ATP‑dependent helicase RecQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730795,9680CT 6.3% 315.9 / 4.8 102R296R (CGC→CGTrecQ1ATP‑dependent helicase RecQ
Reads supporting (aligned to +/- strand):  ref base C (47/48);  new base T (3/4);  total (50/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 103 total aligned reads displayed.

AAAATTGAAAGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATA  >  CP000730/795828‑796106
                                                                                                                                             |                                                                                                                                          
aaaaTTGAAAGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGc                                                                                                                                            <  1:953780/141‑1 (MQ=255)
 aaaTTGAAAGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCt                                                                                                                                           <  1:840128/141‑1 (MQ=255)
 aaaTAGAAAGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCt                                                                                                                                           <  2:463430/141‑1 (MQ=255)
  aaTTGAAAGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCtt                                                                                                                                          <  1:31951/141‑1 (MQ=255)
   aTTGAAAGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCttt                                                                                                                                         <  1:162421/141‑1 (MQ=255)
       aaaGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTGGATCGTGTTAAAGTAGTCGTTGCTACAAATGCGTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTa                                                                                                                                     >  1:591048/1‑141 (MQ=255)
        aaGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTTTGGGTATTGATAAATCCAATGTACGCTTTGTTAt                                                                                                                                    >  1:1008025/1‑141 (MQ=255)
        aaGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTAt                                                                                                                                    >  2:762422/1‑141 (MQ=255)
        aaGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTAt                                                                                                                                    <  1:48460/141‑1 (MQ=255)
        aaGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTAt                                                                                                                                    >  2:354470/1‑141 (MQ=255)
            gtTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCAt                                                                                                                                >  2:785453/1‑141 (MQ=255)
              tatataTCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCAtt                                                                                                                               >  2:521228/1‑141 (MQ=255)
               atatatCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATtat                                                                                                                             <  2:419768/141‑1 (MQ=255)
               atatatCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATtat                                                                                                                             >  2:265469/1‑141 (MQ=255)
                catatCAAGCAGGTTTGAGCAATAAAGAAAGAGCAGCAGCGCAGAATGCGTTCTTATCTGAACGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATtata                                                                                                                            >  2:940270/2‑141 (MQ=255)
                      aTGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGc                                                                                                                      <  2:40606/141‑1 (MQ=255)
                        gCAGGTTTGAGCAATAAAGAAAGAGAAGCAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTCATTCATTATAATATGCCt                                                                                                                    >  2:800753/1‑141 (MQ=255)
                        gCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCt                                                                                                                    >  2:594554/1‑141 (MQ=255)
                         cAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTg                                                                                                                   >  2:1031369/1‑141 (MQ=255)
                          aGGTTTGNGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTgg                                                                                                                  <  2:814830/141‑1 (MQ=255)
                           ggTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGTTTTGTTATTCATTATAATATGCCTGga                                                                                                                 >  2:905649/1‑141 (MQ=255)
                            gTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGgag                                                                                                                >  1:620639/1‑141 (MQ=255)
                               tGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGAtt                                                                                                             <  2:760174/141‑1 (MQ=255)
                                gAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGAttt                                                                                                            <  1:939399/141‑1 (MQ=255)
                                 aGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTa                                                                                                           >  2:269493/1‑141 (MQ=255)
                                   cAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGa                                                                                                         >  2:600634/1‑141 (MQ=255)
                                     aTAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAAt                                                                                                       <  1:310304/141‑1 (MQ=255)
                                     aTAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGAATTGGTAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAAt                                                                                                       <  2:238912/141‑1 (MQ=255)
                                        aagaaagaGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCtc                                                                                                    <  1:1031411/141‑2 (MQ=255)
                                         agaaagaGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCtta                                                                                                   <  1:668133/141‑1 (MQ=255)
                                          gaaagaGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGTTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCttat                                                                                                  >  2:1063317/1‑141 (MQ=255)
                                          gaaagaGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCttat                                                                                                  <  2:430845/141‑1 (MQ=255)
                                            aagaGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCttatta                                                                                                >  1:871444/1‑141 (MQ=255)
                                            aagaGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCttatta                                                                                                >  1:447802/1‑141 (MQ=255)
                                              gagaAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATc                                                                                              <  1:547668/141‑1 (MQ=255)
                                              gagaAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATc                                                                                              >  1:186765/1‑141 (MQ=255)
                                                 aagaagCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCaag                                                                                           <  2:330865/141‑1 (MQ=255)
                                                 aagaagCGCAGAATGATTTCTTATTTGATCGTGTGAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCaag                                                                                           <  1:1004699/141‑1 (MQ=255)
                                                    aagCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCaagaag                                                                                        >  1:541396/1‑141 (MQ=255)
                                                      gcgcAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCg                                                                                      >  1:188963/1‑141 (MQ=255)
                                                         cAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGTGATTTAGAATCTTATTATCAAGAAGCGGGt                                                                                   <  2:975609/141‑1 (MQ=255)
                                                           gAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCGTATTATCAAGAAGCGGGTCg                                                                                 >  1:285583/1‑141 (MQ=255)
                                                             aTGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTc                                                                                                                                  <  1:524512/90‑1 (MQ=255)
                                                             aTGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTc                                                                                                                                  >  2:524512/1‑90 (MQ=255)
                                                               gATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCa                                                                             >  2:201723/1‑141 (MQ=255)
                                                                 tttCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAgg                                                                           >  2:866091/1‑141 (MQ=255)
                                                                 tttCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAgg                                                                           <  1:414782/141‑1 (MQ=255)
                                                                  ttCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGt                                                                          <  2:106962/141‑1 (MQ=255)
                                                                     ttATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGt                                                                       >  2:680936/1‑141 (MQ=255)
                                                                     ttATTTGATCGTGTTAAAGTAGTCGCTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGt                                                                       >  2:334317/1‑141 (MQ=255)
                                                                         ttGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACg                                                                   <  1:672536/141‑1 (MQ=255)
                                                                           gATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACggg                                                                 <  1:47164/141‑1 (MQ=255)
                                                                           gATCGTGGTAAAGTAGTCGTTGCTACAAATACTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACggg                                                                 <  2:88351/141‑1 (MQ=255)
                                                                            aTCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGt                                                                <  2:620639/141‑1 (MQ=255)
                                                                             tCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGtt                                                               <  1:352327/141‑1 (MQ=255)
                                                                             tCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGtt                                                               <  2:731317/141‑1 (MQ=255)
                                                                              cGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGTTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTg                                                              <  1:511532/141‑1 (MQ=255)
                                                                              cGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTg                                                              >  1:129047/1‑141 (MQ=255)
                                                                              cGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTg                                                              <  1:846879/141‑1 (MQ=255)
                                                                               gtgtTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGa                                                             <  2:186948/141‑1 (MQ=255)
                                                                               gtgtTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGa                                                             <  2:312603/141‑1 (MQ=255)
                                                                               gtgtTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGa                                                             <  2:193449/141‑1 (MQ=255)
                                                                               gtgtTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGa                                                             <  2:868647/141‑1 (MQ=255)
                                                                                  ttAAAGAAGTCGTTGCTACAAATGCTTTAGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCGTGGAGATATAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGaaaa                                                          >  2:535226/1‑141 (MQ=255)
                                                                                         agtCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATg                                                   >  2:247339/1‑141 (MQ=255)
                                                                                         agtCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATg                                                   >  1:548840/1‑141 (MQ=255)
                                                                                           tCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTa                                                 >  2:632699/1‑141 (MQ=255)
                                                                                            cGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTAt                                                <  2:217314/141‑1 (MQ=255)
                                                                                             gTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTAtt                                               >  2:731024/1‑141 (MQ=255)
                                                                                              ttGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGCTGAAAAGTGAATGTCttt                                              >  1:817857/1‑141 (MQ=255)
                                                                                               tGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTAtttt                                             <  1:121238/141‑1 (MQ=255)
                                                                                                gCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTg                                            <  2:29439/141‑1 (MQ=255)
                                                                                                 cTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGt                                           <  1:110951/141‑1 (MQ=255)
                                                                                                  tACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGACAAGTGAATGTATTTTGtt                                          >  2:281995/1‑141 (MQ=255)
                                                                                                      aaTGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTAttt                                      >  1:449597/1‑141 (MQ=255)
                                                                                                      aaTGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTAttt                                      >  1:777095/1‑141 (MQ=255)
                                                                                                        tGCTTTTGGTATGGGTATTGATAAATCCAATGTACGTTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAg                                    <  1:1063317/141‑1 (MQ=255)
                                                                                                        tGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTCATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAg                                    <  2:1036573/141‑1 (MQ=255)
                                                                                                         gCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGc                                   <  1:296099/141‑1 (MQ=255)
                                                                                                            ttttGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGa                                 <  2:121624/141‑1 (MQ=255)
                                                                                                             tttGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGaa                                >  2:720667/1‑141 (MQ=255)
                                                                                                             ttcggTATGGGGATTGATAAATCCAATGTACGCTTTGTGATTCATTATAATATGGCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGaa                                <  2:925719/138‑1 (MQ=255)
                                                                                                               tGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAAcg                              >  1:457110/1‑141 (MQ=255)
                                                                                                                ggTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGGAGAACATTCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAAcgc                             >  1:342567/1‑141 (MQ=255)
                                                                                                                 gTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAAcgcg                            >  1:228068/1‑141 (MQ=255)
                                                                                                                 gTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAAcgcg                            >  2:757150/1‑141 (MQ=255)
                                                                                                                  tATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGa                           <  1:453568/141‑1 (MQ=255)
                                                                                                                     gggTATTGATAAATCCAATGTACGTTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGatat                        <  1:607413/141‑1 (MQ=255)
                                                                                                                     gggTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATGTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTGTGTTATTTAGCGAACGCGatat                        >  2:103296/1‑141 (MQ=255)
                                                                                                                          ttGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAAtt                   <  1:878847/141‑1 (MQ=255)
                                                                                                                          ttGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAAtt                   >  2:258638/1‑141 (MQ=255)
                                                                                                                                aaTCCAATGTACGTTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACg             <  2:66922/141‑1 (MQ=255)
                                                                                                                                aaTCCAATGTACGCTTTGTTATTCGTTATAATAGGCCTGGAGATGGAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACg             <  1:864254/141‑1 (MQ=255)
                                                                                                                                aaTCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACg             >  2:1036475/1‑141 (MQ=255)
                                                                                                                                 aTCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGa            <  2:591048/141‑1 (MQ=255)
                                                                                                                                      aTGTACGTTTTGTTATTCATTAAAATATGCCTGGAGATATAGAATATTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGGTATTTAGCGAACGCGATATCAATTTACACGAGTAtt       >  2:920249/1‑141 (MQ=255)
                                                                                                                                      aTGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTAtt       >  1:325247/1‑141 (MQ=255)
                                                                                                                                        gTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTAtttt     <  2:658952/141‑1 (MQ=255)
                                                                                                                                         tACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTta    <  2:637274/141‑1 (MQ=255)
                                                                                                                                           cGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTtata  >  1:217134/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                          
AAAATTGAAAGTGTTATATATCATGCAGGTTTGAGCAATAAAGAAAGAGAAGAAGCGCAGAATGATTTCTTATTTGATCGTGTTAAAGTAGTCGTTGCTACAAATGCTTTTGGTATGGGTATTGATAAATCCAATGTACGCTTTGTTATTCATTATAATATGCCTGGAGATTTAGAATCTTATTATCAAGAAGCGGGTCGTGCAGGTCGTGACGGGTTGAAAAGTGAATGTATTTTGTTATTTAGCGAACGCGATATCAATTTACACGAGTATTTTATA  >  CP000730/795828‑796106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: