BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	864,828	G→A	5.7%	P48P (CCG→CCA)	est →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	864,828	0	G	A	5.7%	323.3 / 6.3	104	P48P (CCG→CCA)	est	carboxylesterase
Reads supporting (aligned to +/- strand): ref base G (53/45); new base A (3/3); total (56/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01

Only 100 of 106 total aligned reads displayed.

CAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGCTGGTCTATCA  >  CP000730/864688‑864963

cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCg                                                                                                                                         <  1:722474/141‑1 (MQ=255)

cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTATAGTGTTATTACTACATGTTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAAAAAGGTTATACATCATATGCACCg                                                                                                                                         <  2:11662/141‑1 (MQ=255)

 aGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                                                                                                                        <  2:104898/141‑1 (MQ=255)

    tAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAat                                                                                                                                     >  2:345063/1‑141 (MQ=255)

      aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                                                                                                                                   >  1:1075010/1‑141 (MQ=255)

      aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                                                                                                                                   >  1:440928/1‑141 (MQ=255)

         ttACCAAAACCTTTCTTTTTTTTTTTTTTTTAACGTGCAGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGGTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGATATACATCATATGTACTGCAATATGaa                                                                                                                                >  2:488527/1‑141 (MQ=255)

           aCCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAgg                                                                                                                              <  1:78141/141‑1 (MQ=255)

           aCCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAgg                                                                                                                              <  1:78101/141‑1 (MQ=255)

            ccAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTGGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGc                                                                                                                             >  1:1083375/1‑141 (MQ=255)

             cAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                                                                                                                            <  1:425089/141‑1 (MQ=255)

              aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                                                                                                                           >  1:653824/1‑141 (MQ=255)

              aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                                                                                                                           >  1:109040/1‑141 (MQ=255)

               aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCGATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                                                                                                                          <  1:203537/141‑1 (MQ=255)

               aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                                                                                                                          >  1:514729/1‑141 (MQ=255)

                aaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcg                                                                                                                         <  2:1042963/141‑1 (MQ=255)

                 aCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                                                                                                        <  1:351197/141‑1 (MQ=255)

                 aCCCTTCTTTTTTGAGGCAGGTAAACGTGCCGTGTTATTACTACGTGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                                                                                                                        <  2:94109/141‑1 (MQ=255)

                    tttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGc                                                                                                                     <  2:400712/141‑1 (MQ=255)

                     ttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGca                                                                                                                    <  1:254814/141‑1 (MQ=255)

                      tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                                                                                                   >  1:335442/1‑141 (MQ=255)

                      tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                                                                                                                   <  1:672670/141‑1 (MQ=255)

                        ttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcacca                                                                                                                 >  1:410589/1‑141 (MQ=255)

                           tttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCAccacca                                                                                                              <  1:794246/141‑1 (MQ=255)

                             tGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGa                                                                                                            >  2:763046/1‑141 (MQ=255)

                               aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg                                                                                                          <  1:717283/141‑1 (MQ=255)

                               aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg                                                                                                          >  2:153131/1‑141 (MQ=255)

                                ggAAGGTAAACGTCCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGa                                                                                                         <  1:177527/141‑1 (MQ=255)

                                 ttttttAAACGTGCCGTGTGATTACTACATGGTTTTACAGGGAATTCGTCTGATGGTGGTCAATTAGGTCGATTTTTATTAATGAAATGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCATCACCAGATGag                                                                                                        >  2:582879/6‑140 (MQ=255)

                                 gAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaa                                                                                                        <  1:89797/141‑1 (MQ=255)

                                 gAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaa                                                                                                        <  1:89841/141‑1 (MQ=255)

                                     gTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATAc                                                                                                    <  2:142713/141‑1 (MQ=255)

                                       aaaCGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTg                                                                                                  <  1:672945/141‑1 (MQ=255)

                                        aaCGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGa                                                                                                 <  2:404359/141‑1 (MQ=255)

                                         aCGTGCCGTGTTATTATTACATGGATTTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAGGTCACGCGGCACCACCAGAGGAAATACTGaa                                                                                                >  2:728196/1‑141 (MQ=255)

                                          cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                                                                                               >  1:1026470/1‑141 (MQ=255)

                                          cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                                                                                               >  1:186789/1‑141 (MQ=255)

                                          cGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGaaa                                                                                               >  1:180587/1‑141 (MQ=255)

                                                 tgtTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTc                                                                                        >  2:344917/1‑141 (MQ=255)

                                                  gtTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTcc                                                                                       <  1:16966/141‑1 (MQ=255)

                                                   ttattaCTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCt                                                                                      <  1:277740/141‑1 (MQ=255)

                                                    tattaCTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTAAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCCCGCGGCACCACCAGATGAAATACTGAAATCTAGTCCtt                                                                                     <  2:499654/141‑1 (MQ=255)

                                                      ttaCTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTc                                                                                   >  2:960032/1‑141 (MQ=255)

                                                       tactacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCg                                                                                  >  1:869182/1‑141 (MQ=255)

                                                         ctacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt                                                                                <  1:159298/141‑1 (MQ=255)

                                                         ctacATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGtt                                                                                <  1:196574/141‑1 (MQ=255)

                                                           acATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTg                                                                              <  1:221603/141‑1 (MQ=255)

                                                           acATGGTTTTACAGGCAAGTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTg                                                                              <  2:792921/141‑1 (MQ=255)

                                                              tGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGtt                                                                           <  1:631514/141‑1 (MQ=255)

                                                                     aCAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGGTTGGTTTAAAGAt                                                                    >  2:123836/1‑141 (MQ=255)

                                                                        ggCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCg                                                                 >  2:173053/1‑141 (MQ=255)

                                                                        ggCAATCCGTCTGCTGTTCGTCAATTAGGTCGATTTTTACAAAAGAACGGTTATACATCATCTGCACCGCAATATGAAGGCCACGCGGCACCACCAGCTGACATACTGAAATCTAGTCCTCTCCTTTCGTTTAAAGATGCg                                                                 >  2:373804/1‑141 (MQ=255)

                                                                         gCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGt                                                                >  1:41959/1‑141 (MQ=255)

                                                                         gCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGt                                                                <  1:842588/141‑1 (MQ=255)

                                                                                gTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATgg                                                         >  2:96157/1‑141 (MQ=255)

                                                                                gTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATgg                                                         <  2:180587/141‑1 (MQ=255)

                                                                                 tCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAGGTCACGCGGCACCACCAGAGGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGt                                                        >  2:344925/1‑141 (MQ=255)

                                                                                  cTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGtt                                                       <  1:578806/141‑1 (MQ=255)

                                                                                  cTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGtt                                                       >  1:774261/1‑141 (MQ=255)

                                                                                    gaggTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTAt                                                     <  2:325558/138‑1 (MQ=255)

                                                                                    gATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTAt                                                     <  2:325545/141‑1 (MQ=255)

                                                                                    gATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTAt                                                     <  1:827544/141‑1 (MQ=255)

                                                                                       gTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGAt                                                  <  2:743215/141‑1 (MQ=255)

                                                                                       gTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGAt                                                  <  1:152730/141‑1 (MQ=255)

                                                                                          cGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTAt                                               >  2:1051033/1‑141 (MQ=255)

                                                                                            tCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCt                                             >  2:638057/1‑141 (MQ=255)

                                                                                            tCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCt                                             >  2:638092/1‑141 (MQ=255)

                                                                                             cAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCtt                                            >  1:866848/1‑141 (MQ=255)

                                                                                                ttAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCttgtt                                         >  2:364302/1‑141 (MQ=255)

                                                                                                   ggTCGATTTTTACAAAAGCAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGaa                                      <  2:487251/141‑1 (MQ=255)

                                                                                                    gTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAAc                                     >  2:605678/1‑141 (MQ=255)

                                                                                                    gTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAAc                                     <  1:1015686/141‑1 (MQ=255)

                                                                                                    gTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAAc                                     >  2:632118/1‑141 (MQ=255)

                                                                                                     tCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACa                                    >  2:680759/1‑141 (MQ=255)

                                                                                                      cGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACaa                                   <  1:476276/141‑1 (MQ=255)

                                                                                                       gATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAg                                  >  1:892203/1‑141 (MQ=255)

                                                                                                       gATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAg                                  >  2:905670/1‑141 (MQ=255)

                                                                                                         tttttACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGt                                >  2:1034294/1‑141 (MQ=255)

                                                                                                         tttttACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGt                                >  1:516994/1‑141 (MQ=255)

                                                                                                         tttttACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGt                                >  2:741077/1‑141 (MQ=255)

                                                                                                             tACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTatg                            <  1:605678/141‑1 (MQ=255)

                                                                                                              ttttttttttttttATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTatga                           >  2:874330/13‑141 (MQ=255)

                                                                                                              aCAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACAACCAGATGAAATACTGAAATCTAGTCCTTTCGCTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTaaga                           >  2:866476/1‑141 (MQ=255)

                                                                                                               cAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTatgat                          <  2:104512/141‑1 (MQ=255)

                                                                                                               cAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTatgat                          <  1:815829/141‑1 (MQ=255)

                                                                                                                    gaaagGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAAt                     <  1:325608/141‑1 (MQ=255)

                                                                                                                       agGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAAttgt                  >  2:58363/1‑141 (MQ=255)

                                                                                                                        ggTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAAttgtt                 >  1:244696/1‑141 (MQ=255)

                                                                                                                         gatataCTTCGTATGCACCACAATATGAAGGTCACGCGGTACCACCAGAGGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAAttgttg                >  2:401364/3‑141 (MQ=255)

                                                                                                                         gTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAAttgttg                >  2:335272/1‑141 (MQ=255)

                                                                                                                         gTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAAttgttg                <  1:731571/141‑1 (MQ=255)

                                                                                                                             taCATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGc            <  1:331368/141‑1 (MQ=255)

                                                                                                                              aCATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGCt           >  1:665412/1‑141 (MQ=255)

                                                                                                                              aCATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGCt           >  2:407499/1‑141 (MQ=255)

                                                                                                                                 tcatATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGCTGGt        >  2:1046434/1‑141 (MQ=255)

                                                                                                                                  cgtatGCACCACAATATGAAGGTCACGCGGCACCACCAGAGGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGCTGGTc       <  1:910009/139‑1 (MQ=255)

                                                                                                                                   atatGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGCTGGTCt      >  2:1011605/1‑141 (MQ=255)

                                                                                                                                   atatGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGCTGGTCt      <  1:845134/141‑1 (MQ=255)

                                                                                                                                       gCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGCTGGTCTATCa  >  1:308582/1‑141 (MQ=255)

                                                                                                                                       gCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGCTGGTCTATCa  >  2:115941/1‑141 (MQ=255)

CAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTTTTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATACTGAAATCTAGTCCTTTCGTTTGGTTTAAAGATGCGTTAGATGGTTATGATTATCTTGTTGAACAAGGTTATGATGAAATTGTTGTTGCTGGTCTATCA  >  CP000730/864688‑864963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`