Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	937,158	T→A	5.3%	intergenic (+65/‑1952)	yumB → / → USA300HOU_0903	NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	937,158	0	T	A	5.3%	263.5 / 4.7	88	intergenic (+65/‑1952)	yumB/USA300HOU_0903	NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (46/37);  new base A (3/2);  total (49/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAA  >  CP000730/937021‑937297                                                                                                                                           |                                                                                                                                            aaaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCAc                                                                                                                                          >  1:524111/1‑141 (MQ=255) aaaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGacac                                                                                                                                          <  1:1075985/141‑1 (MQ=255)  aaaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACt                                                                                                                                         <  1:1008379/141‑1 (MQ=255)   aaGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTg                                                                                                                                        >  2:153970/1‑141 (MQ=255)    aGTGATTGATACACGTGCGGTATTCAAAATAGGTGGTATCGGTTTAAAAATCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGa                                                                                                                                       <  2:651729/141‑1 (MQ=255)     gTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGaa                                                                                                                                      >  1:840856/1‑141 (MQ=255)       gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                                    <  1:841687/141‑1 (MQ=255)       gattgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAgg                                                                                                                                    >  2:779370/1‑141 (MQ=255)         ttgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGc                                                                                                                                  >  2:497608/1‑141 (MQ=255)         ttgatACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGc                                                                                                                                  <  1:1083044/141‑1 (MQ=255)             tACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAGTGGCTTTa                                                                                                                              >  2:634268/1‑141 (MQ=255)             tACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAGGGGCTTTa                                                                                                                              >  2:580770/1‑141 (MQ=255)                 cGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTAttgt                                                                                                                          >  2:949608/1‑141 (MQ=255)                 cGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTAttgt                                                                                                                          >  2:704566/1‑141 (MQ=255)                    gCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATtgttgt                                                                                                                       <  2:619975/141‑1 (MQ=255)                     cGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTa                                                                                                                      <  1:118769/141‑1 (MQ=255)                      ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAc                                                                                                                     <  1:688015/141‑1 (MQ=255)                      ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTAc                                                                                                                     <  2:133603/141‑1 (MQ=255)                      ggTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGCCCTTCAGTGACACTGAAGGACTTTAtaaatgaat                                                                                                                     <  1:433477/141‑9 (MQ=38)                       gTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACa                                                                                                                    <  1:502115/141‑1 (MQ=255)                       gTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACa                                                                                                                    <  1:252870/141‑1 (MQ=255)                         atccAAAATCGGTGGTATCGGGCTAGCATTCAAAAAAGGTAAATTCTAGGCTCTAAATAAAGTTTCAGCTACAACA‑AAACCACTACCCTATTCAATTATACAGTCCTCCAGTGTCACAGAAGGGCCTCATTGTTGTACAAt                                                                                                                  >  1:810909/4‑141 (MQ=255)                           tCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATGCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAAttt                                                                                                                <  1:247407/141‑1 (MQ=255)                           tCAAAAACGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCATTATGACTGAAGGGCTTTATTGTTGTACAAttt                                                                                                                <  1:524374/141‑1 (MQ=255)                             aaaaTCGGTGGTATCGGGTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGa                                                                                                              <  2:128582/141‑1 (MQ=255)                               aaTCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAg                                                                                                            <  1:122687/141‑1 (MQ=255)                                 tCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTg                                                                                                          <  1:632059/141‑1 (MQ=255)                                 tCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTg                                                                                                          <  1:54804/141‑1 (MQ=255)                                   ggtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAg                                                                                                        >  1:1075732/1‑141 (MQ=255)                                    gtggtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGt                                                                                                       >  2:945286/1‑141 (MQ=255)                                       gtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAAc                                                                                                    >  2:142962/1‑141 (MQ=255)                                       gtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAAc                                                                                                    <  1:69414/141‑1 (MQ=255)                                       gtATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAAc                                                                                                    >  1:251468/1‑141 (MQ=255)                                              tttAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCaa                                                                                             >  1:443527/1‑141 (MQ=255)                                                     ttCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAag                                                                                      <  1:308663/141‑1 (MQ=255)                                                     ttCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAag                                                                                      >  1:202383/1‑141 (MQ=255)                                                        aaaaaaGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAaggag                                                                                   <  2:793941/141‑1 (MQ=255)                                                                 aaaTTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTAt                                                                          >  1:710926/1‑141 (MQ=255)                                                                  aaTTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                         <  1:651599/141‑1 (MQ=255)                                                                  aaTTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                         >  1:311548/1‑141 (MQ=255)                                                                  aaTTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATg                                                                         >  2:836199/1‑141 (MQ=255)                                                                       tAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAAttt                                                                    <  2:503052/141‑1 (MQ=255)                                                                       tAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAAttt                                                                    <  1:447851/141‑1 (MQ=255)                                                                         ggCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTtta                                                                  >  1:586357/1‑141 (MQ=255)                                                                         ggCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTtta                                                                  >  2:619698/1‑141 (MQ=255)                                                                         ggCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTtta                                                                  >  1:838764/1‑141 (MQ=255)                                                                          gCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaa                                                                 <  1:569153/141‑1 (MQ=255)                                                                            tttAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaat                                                               >  2:748648/1‑141 (MQ=255)                                                                             ttAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTttaaatt                                                              >  1:418190/1‑141 (MQ=255)                                                                                    aaGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAac                                                       <  1:64280/141‑1 (MQ=255)                                                                                     aGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAaca                                                      >  2:133610/1‑141 (MQ=255)                                                                                       tttCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAacaca                                                    <  1:35211/141‑1 (MQ=255)                                                                                         tCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACt                                                  <  1:1002740/141‑1 (MQ=255)                                                                                         tCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACt                                                  >  1:777628/1‑141 (MQ=255)                                                                                          cAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCGGAGTCACTGAAGGGCTTTATTGTTGAACAATTCGAAGGGAGTAACATATCAAACCAAAGGTGGTACATTATGAATATGAAATTAAAGAACCCACTa                                                 >  2:317858/1‑141 (MQ=255)                                                                                          cAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTa                                                 >  1:810604/1‑141 (MQ=255)                                                                                          cAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACAGTATGAATTTTAAATTAAATAACACAATa                                                 >  2:779242/1‑141 (MQ=255)                                                                                            gCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAg                                               >  2:107386/1‑141 (MQ=255)                                                                                               a‑aaCACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCaa                                            >  2:929488/1‑141 (MQ=255)                                                                                                 aaCACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAAc                                           <  1:473900/141‑1 (MQ=255)                                                                                                  acacATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACg                                          <  1:467235/141‑1 (MQ=255)                                                                                                     caTAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaa                                       <  2:278092/141‑1 (MQ=255)                                                                                                        aaaaTTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGaaataa                                    <  1:390789/141‑1 (MQ=255)                                                                                                          aattaaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGGTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTACGCAACGaaataaat                                  >  2:687120/1‑141 (MQ=255)                                                                                                              aaattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACAt                              >  1:315115/1‑141 (MQ=255)                                                                                                               aattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGCAATAAATACAtt                             >  1:981937/1‑141 (MQ=255)                                                                                                               aattaTTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACAtt                             >  2:203185/1‑141 (MQ=255)                                                                                                                  tattCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGat                          >  1:978344/1‑141 (MQ=255)                                                                                                                     tCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGATACATTATGAATTTTAAATTAAATAACACACTAAGAAACAAAATAAATACATTGattaa                       >  2:506109/1‑140 (MQ=255)                                                                                                                      cATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGattatt                      >  2:558906/1‑141 (MQ=255)                                                                                                                      cATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGattatt                      >  2:872431/1‑141 (MQ=255)                                                                                                                        tttATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                    <  2:828285/141‑1 (MQ=255)                                                                                                                        tttATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGAAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTgg                    >  1:601787/1‑141 (MQ=255)                                                                                                                         ttATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGt                   <  1:128193/141‑1 (MQ=255)                                                                                                                           ataAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGtat                 <  1:622432/141‑1 (MQ=255)                                                                                                                           ataAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGtat                 >  2:287715/1‑141 (MQ=255)                                                                                                                            taAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGtata                >  1:840854/1‑141 (MQ=255)                                                                                                                             aaaGTGCTTCAGTGTCACTGAAGGGATTTATTTTTGTACAATTTGAAGTGTGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAc               <  2:822212/141‑1 (MQ=255)                                                                                                                              aaGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATAcc              <  1:663640/141‑1 (MQ=255)                                                                                                                                gcccTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACCTTGATTATTGGtatgcgag            >  2:896742/3‑136 (MQ=255)                                                                                                                                gcccTTCAGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg            >  2:284797/3‑141 (MQ=255)                                                                                                                                gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACAGACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg            >  1:10237/1‑141 (MQ=255)                                                                                                                                gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg            <  2:750006/141‑1 (MQ=255)                                                                                                                                gTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAg            >  1:660052/1‑141 (MQ=255)                                                                                                                                 tCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGa           >  1:1087315/1‑141 (MQ=255)                                                                                                                                   tttCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAAc         <  2:911408/140‑1 (MQ=255)                                                                                                                                   cTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAAc         >  2:718308/1‑141 (MQ=255)                                                                                                                                       aGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACAttt     <  1:821446/141‑1 (MQ=255)                                                                                                                                       aGTGACACTGAAGGACTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACAttt     >  1:257931/1‑141 (MQ=255)                                                                                                                                        gtgtCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTa    >  2:728696/1‑141 (MQ=255)                                                                                                                                          ttCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTaaa  <  2:932264/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                            AAAAGTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGTAAATTCTAGGCTTTAAATAAAGTTTCAGCTA‑AACACATAAAATTAAATTATTCATTTATAAAGTCCTTCAGTGTCACTGAAGGGCTTTATTGTTGTACAATTTGAAGTGAGTAACATATCAAATCTAAGGAGGTACATTATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATACCAGAACATTTAAA  >  CP000730/937021‑937297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑