Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 946,579 A→C 5.4% F488L (TTT→TTG mnhA2 ← CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730946,5790AC 5.4% 302.5 / 8.1 93F488L (TTT→TTGmnhA2CPA3 family monovalent cation:proton (H+) antiporter‑3 subunit A
Reads supporting (aligned to +/- strand):  ref base A (55/33);  new base C (2/3);  total (57/36)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ATATAAATCACTAATGTTGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAATTTAATTGAATATACAAATGT  >  CP000730/946439‑946719
                                                                                                                                            |                                                                                                                                            
atatAAATCACTAATGTTGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCa                                                                                                                                              <  1:665416/141‑1 (MQ=255)
  ataAATCACTAATGTTGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCaaa                                                                                                                                            >  2:265735/1‑141 (MQ=255)
    aaaTCACTAATGTTGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATa                                                                                                                                          <  1:274525/141‑1 (MQ=255)
     aaTCACTAATGTTGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATAc                                                                                                                                         >  2:508412/1‑141 (MQ=255)
     aaTCACTAATGTTGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATAc                                                                                                                                         >  1:156211/1‑141 (MQ=255)
       tCACTAATGTTGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACaa                                                                                                                                       <  2:50440/141‑1 (MQ=255)
           tAATGTTGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTAc                                                                                                                                   >  1:700248/1‑141 (MQ=255)
                ttGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATg                                                                                                                              <  1:480984/141‑1 (MQ=255)
                ttGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATg                                                                                                                              <  1:319702/141‑1 (MQ=255)
                aaaaaaaaaaGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATg                                                                                                                              <  2:1014233/136‑1 (MQ=255)
                 tGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGt                                                                                                                             <  2:689694/141‑1 (MQ=255)
                  gATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTa                                                                                                                            >  2:469613/1‑141 (MQ=255)
                   aTAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAg                                                                                                                           >  1:774663/1‑141 (MQ=255)
                     aaaaaGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCt                                                                                                                         <  1:53535/141‑1 (MQ=255)
                     aaaaaGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCt                                                                                                                         <  1:857056/141‑1 (MQ=255)
                         aggcaggcGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTaaaa                                                                                                                     >  1:153000/1‑141 (MQ=255)
                         aggcaggcGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTaaaa                                                                                                                     <  1:180068/141‑1 (MQ=255)
                           gcaggcGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATa                                                                                                                   >  1:1031279/1‑141 (MQ=255)
                           gcaggcGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATa                                                                                                                   >  1:1026396/1‑141 (MQ=255)
                           gcaggcGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATa                                                                                                                   >  1:776804/1‑141 (MQ=255)
                             aggcGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGc                                                                                                                 <  1:691257/141‑1 (MQ=255)
                             aggcGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGc                                                                                                                 >  2:20833/1‑141 (MQ=255)
                                      tCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATaa                                                                                                        >  1:353289/1‑141 (MQ=255)
                                         aTGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAg                                                                                                     <  2:106970/141‑1 (MQ=255)
                                            gAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCAt                                                                                                  <  2:525341/141‑1 (MQ=255)
                                            gAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCAt                                                                                                  <  1:707460/141‑1 (MQ=255)
                                                 tatGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAata                                                                                             >  1:653497/1‑141 (MQ=255)
                                                  atGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAatat                                                                                            >  1:327398/1‑141 (MQ=255)
                                                   tGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATAtt                                                                                           >  1:63490/1‑141 (MQ=255)
                                                    ggAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTg                                                                                          >  1:742192/1‑141 (MQ=255)
                                                    ggAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTg                                                                                          >  1:742187/1‑141 (MQ=255)
                                                            aCATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCg                                                                                  >  2:285960/1‑141 (MQ=255)
                                                            aCATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCg                                                                                  >  1:386142/1‑141 (MQ=255)
                                                               tcatcaATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCgtgt                                                                               >  2:1083228/1‑141 (MQ=255)
                                                               tcatcaATTACAGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAACGCATAAATATTGATATTTCgtgt                                                                               >  2:973303/1‑141 (MQ=255)
                                                                 atcaATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGc                                                                             >  1:369188/1‑141 (MQ=255)
                                                                 atcaATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGAAATCATATTGATACTTCGTGTGc                                                                             >  1:9763/1‑141 (MQ=255)
                                                                    aaTTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCCAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCttt                                                                          >  2:435562/1‑141 (MQ=255)
                                                                    aaTTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCttt                                                                          >  2:428288/1‑141 (MQ=255)
                                                                         ccGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAg                                                                     <  2:582756/141‑1 (MQ=255)
                                                                           gTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGt                                                                   >  2:147530/1‑141 (MQ=255)
                                                                            tATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTa                                                                  <  2:296276/141‑1 (MQ=255)
                                                                             aTGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTaa                                                                 >  1:854416/1‑141 (MQ=255)
                                                                               gATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAAtt                                                               <  1:572662/141‑1 (MQ=255)
                                                                               gATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAAtt                                                               <  1:282955/141‑1 (MQ=255)
                                                                                 ttAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAATCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGt                                                             <  2:316401/141‑1 (MQ=255)
                                                                                 ttAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGt                                                             <  2:451028/141‑1 (MQ=255)
                                                                                 ttAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGt                                                             <  1:952832/141‑1 (MQ=255)
                                                                                  tAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGtt                                                            <  1:808919/141‑1 (MQ=255)
                                                                                     tCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAg                                                         >  2:127462/1‑141 (MQ=255)
                                                                                     tCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAg                                                         >  2:413856/1‑141 (MQ=255)
                                                                                       gAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAACCCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGt                                                       <  2:827541/141‑1 (MQ=255)
                                                                                        aaGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGtt                                                      <  2:34753/141‑1 (MQ=255)
                                                                                        aaGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGtt                                                      <  2:946278/141‑1 (MQ=255)
                                                                                         aGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGttt                                                     >  1:871225/1‑141 (MQ=255)
                                                                                         aGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGttt                                                     <  1:56985/141‑1 (MQ=255)
                                                                                          gATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTta                                                    >  2:932375/1‑141 (MQ=255)
                                                                                          gATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTta                                                    <  1:138782/141‑1 (MQ=255)
                                                                                           aTGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTtat                                                   >  2:386312/1‑141 (MQ=255)
                                                                                              tAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATGCTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATAtt                                                >  1:800289/1‑141 (MQ=255)
                                                                                              tAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATAtt                                                >  2:733473/1‑141 (MQ=255)
                                                                                              tAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATAtt                                                >  1:453925/1‑141 (MQ=255)
                                                                                                gCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGt                                              <  2:732901/141‑1 (MQ=255)
                                                                                                gCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGt                                              >  1:742100/1‑141 (MQ=255)
                                                                                                gCTGGTTCAATAATCGAATTAGTTAAAATGCCAGCGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGt                                              <  1:1007354/141‑1 (MQ=255)
                                                                                                  tGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTcc                                            >  1:693752/1‑141 (MQ=255)
                                                                                                    gTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGa                                          <  1:138685/141‑1 (MQ=255)
                                                                                                    gTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGa                                          >  2:529697/1‑141 (MQ=255)
                                                                                                        aataatCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGaaaaa                                      >  2:998601/1‑141 (MQ=255)
                                                                                                        aataatCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGaaaaa                                      >  2:565695/1‑141 (MQ=255)
                                                                                                        aataatCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGaaaaa                                      >  2:1975/1‑141 (MQ=255)
                                                                                                          taatCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAata                                    >  1:49638/1‑141 (MQ=255)
                                                                                                           aatCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAatat                                   >  1:25144/1‑141 (MQ=255)
                                                                                                           aatCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAatat                                   >  2:429862/1‑141 (MQ=255)
                                                                                                            atCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAatata                                  >  2:287655/1‑141 (MQ=255)
                                                                                                                 aTTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCAt                             <  1:111825/141‑1 (MQ=255)
                                                                                                                 aTTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCAt                             <  1:199771/141‑1 (MQ=255)
                                                                                                                 aTTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCAt                             >  1:438509/1‑141 (MQ=255)
                                                                                                                 aTTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCAt                             >  1:263654/1‑141 (MQ=255)
                                                                                                                   tAGTTAAAATGCCAGGGAATAAACCCAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATta                           <  1:498248/141‑1 (MQ=255)
                                                                                                                    aGTTAAAATGCCAGGGAATAAACCCAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATtat                          >  2:261490/1‑141 (MQ=255)
                                                                                                                    aGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATtat                          >  1:196206/1‑141 (MQ=255)
                                                                                                                     gTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATtata                         <  1:916206/141‑1 (MQ=255)
                                                                                                                     gTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATtata                         >  2:449328/1‑141 (MQ=255)
                                                                                                                      ttAAAATGCCAGGGAATAAACCCAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATaa                        <  1:45847/141‑1 (MQ=255)
                                                                                                                      ttAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATaa                        >  1:585827/1‑141 (MQ=255)
                                                                                                                      ttAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATaa                        >  1:885237/1‑141 (MQ=255)
                                                                                                                        aaaaTGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAAt                      >  2:77791/1‑141 (MQ=255)
                                                                                                                        aaaaTGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAAt                      <  1:91874/141‑1 (MQ=255)
                                                                                                                                 gggAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAATTTAATTGaa             >  2:162388/1‑141 (MQ=255)
                                                                                                                                      tAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAATTTAATTGAATATAc        >  1:498141/1‑141 (MQ=255)
                                                                                                                                        aaCCCAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAATTTAATTGAATATACaa      <  1:84151/141‑1 (MQ=255)
                                                                                                                                         aCCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAATTTAATTGAATATACaaa     >  1:167751/1‑141 (MQ=255)
                                                                                                                                         aCCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAATTTAATTGAATATACaaa     >  2:79594/1‑141 (MQ=255)
                                                                                                                                           cAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAATTTAATTGAATATACAAAtg   >  1:445145/1‑141 (MQ=255)
                                                                                                                                            aaaTACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAATTTAATTGAATATACAAAtgt  <  2:156211/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATATAAATCACTAATGTTGATAAAAAGGCAGGCGTTAATCCATGGAACATATGGAATTCAACATCATCAATTACCGTATGATTAATCGAAGATGTAGCTGGTTCAATAATCGAATTAGTTAAAATGCCAGGGAATAAACCAAATACAATTACTAATGTAGCTAAAATAGCTGGTGATAAAAGCATTAATATTGATACTTCGTGTGCTTTTTTAGGTAATTGTTCAGGTTTATATTGTCCGAAAAATATATGCATTATAAATTTAATTGAATATACAAATGT  >  CP000730/946439‑946719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: