Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 985,069 A→T 7.0% intergenic (‑134/‑161) USA300HOU_0940 ← / → fabH1 hypothetical protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730985,0690AT 7.0% 260.8 / 9.3 86intergenic (‑134/‑161)USA300HOU_0940/fabH1hypothetical protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase
Reads supporting (aligned to +/- strand):  ref base A (43/37);  new base T (4/2);  total (47/39)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

GTTTCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAATTT  >  CP000730/984929‑985209
                                                                                                                                            |                                                                                                                                            
gtTTCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTaaa                                                                                                                                              >  2:1074593/1‑141 (MQ=255)
    cATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTaa                                                                                                                                          >  1:69784/1‑141 (MQ=255)
      tGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAgc                                                                                                                                        <  2:96308/141‑1 (MQ=255)
         cATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCa                                                                                                                                     >  2:684279/1‑141 (MQ=255)
         cATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCa                                                                                                                                     <  1:185184/141‑1 (MQ=255)
          aTTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCAt                                                                                                                                    <  1:570883/141‑1 (MQ=255)
            tCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTa                                                                                                                                  >  1:399057/1‑141 (MQ=255)
                ccTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTaaagc                                                                                                                              >  1:391161/1‑139 (MQ=255)
                ccTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAAc                                                                                                                              >  1:337538/1‑141 (MQ=255)
                ccTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAAc                                                                                                                              >  1:388776/1‑141 (MQ=255)
                 cTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAAcc                                                                                                                             <  2:719926/141‑1 (MQ=255)
                      cTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAAttt                                                                                                                        >  1:966606/1‑141 (MQ=255)
                           attaactatatTTTATTTTCAGAGAAAGTTAGAAATTTGAATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATTATTTTATCTGAATGCCTATTCTAAATTAAGCGCATTAAAACCAATTTCATAc                                                                                                                   >  2:503127/7‑141 (MQ=255)
                            ttATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACt                                                                                                                  <  1:522745/141‑1 (MQ=255)
                              atatatatTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGa                                                                                                                >  1:133240/1‑141 (MQ=255)
                               tttatatTTTATTTGAAGTGAAAGTTTGAAATTTGTATAGTAACATCTCATATATTTTGACCTTATTATTCAGTTTTAATAAATGATTTTATCTTAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGat                                                                                                               <  2:724159/139‑2 (MQ=255)
                               tatatatTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGaa                                                                                                               <  1:1012059/141‑1 (MQ=255)
                                   tattttatttTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAGATTAAGCGCATTAAAGCCAATTTCATACTGAAAttt                                                                                                           <  2:291195/141‑1 (MQ=255)
                                      tttattttaAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGAc                                                                                                        <  1:36176/141‑1 (MQ=255)
                                       ttattttaAGTGAAAGTTAGAAATTTGTATAGTAACATCTAATATATTTTGACCATATTATAGAGTTTAACTAAATGATTTTATCTGAATGGCTATTATAAATTGCGCGCATTAAAACCACTTTCATACTGAAATTTGACg                                                                                                       <  2:500345/141‑1 (MQ=255)
                                       ttattttaAGTGAAAGTTAGAAAATTGTATAGCAACATCTTATATATTTAGATGATACTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACg                                                                                                       <  1:1024935/141‑1 (MQ=255)
                                               aGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATaaa                                                                                               >  2:734452/1‑141 (MQ=255)
                                               aGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATaaa                                                                                               >  1:888025/1‑141 (MQ=255)
                                               aGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATaaa                                                                                               >  2:236051/1‑141 (MQ=255)
                                                    aaGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCAtt                                                                                          >  1:99610/1‑141 (MQ=255)
                                                      gTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaa                                                                                        <  2:829989/141‑1 (MQ=255)
                                                      gTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaa                                                                                        >  1:174001/1‑141 (MQ=255)
                                                       ttAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaaa                                                                                       >  1:609298/1‑141 (MQ=255)
                                                         aGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAt                                                                                     >  2:317137/1‑141 (MQ=255)
                                                         aGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAt                                                                                     >  1:538361/1‑141 (MQ=255)
                                                          gAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAtt                                                                                    >  2:10429/1‑141 (MQ=255)
                                                          gAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAtt                                                                                    <  1:127921/141‑1 (MQ=255)
                                                             aTTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTtta                                                                                 >  2:115767/1‑141 (MQ=255)
                                                             aTTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTtta                                                                                 >  1:1045392/1‑141 (MQ=255)
                                                              tttGTNTAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCANACTNAAATTTGACGATAATAAAGCATTAAAATTttat                                                                                <  1:384154/141‑1 (MQ=255)
                                                              tttGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttat                                                                                <  1:1074593/141‑1 (MQ=255)
                                                               ttGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttatt                                                                               >  1:162250/1‑141 (MQ=255)
                                                                tGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttatta                                                                              <  2:696868/141‑1 (MQ=255)
                                                                tGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttatta                                                                              >  2:172757/1‑141 (MQ=255)
                                                                tGTATAGTAACATCTCATATATTTTGAACATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATACAGCATTCCCATTttatta                                                                              >  2:643959/1‑141 (MQ=255)
                                                                 gTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTaa                                                                             >  2:907418/1‑141 (MQ=255)
                                                                  tataGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAAc                                                                            <  1:375670/141‑1 (MQ=255)
                                                                     aGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGTATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAg                                                                         <  2:286802/141‑1 (MQ=255)
                                                                     aGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAg                                                                         <  1:1066210/141‑1 (MQ=255)
                                                                     aGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAg                                                                         <  2:144709/141‑1 (MQ=255)
                                                                      gTAACATCTCATATATTTTGACCATATTATACAGTTTAACTAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGt                                                                        <  1:799932/141‑1 (MQ=255)
                                                                      gTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGt                                                                        >  2:621054/1‑141 (MQ=255)
                                                                         aCATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCaa                                                                     <  1:172757/141‑1 (MQ=255)
                                                                            tctcATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAatat                                                                  >  1:406460/1‑141 (MQ=255)
                                                                                    atTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACct                                                          <  2:396365/141‑1 (MQ=255)
                                                                                    atTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACct                                                          <  1:184588/141‑1 (MQ=255)
                                                                                     ttttGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACctt                                                         <  1:1030670/141‑2 (MQ=255)
                                                                                     ttttGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACctc                                                         >  2:1018622/1‑141 (MQ=255)
                                                                                       ttGATCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTg                                                       <  2:866851/141‑1 (MQ=255)
                                                                                             atatTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGa                                                 <  1:968526/141‑1 (MQ=255)
                                                                                             atatTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGa                                                 <  1:995026/141‑1 (MQ=255)
                                                                                             atatTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGa                                                 <  2:607920/141‑1 (MQ=255)
                                                                                              tattttACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCATTTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAg                                                >  2:287354/1‑141 (MQ=255)
                                                                                                ttatACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGtt                                              >  1:286453/1‑141 (MQ=255)
                                                                                                 tataCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGttt                                             >  1:976615/1‑141 (MQ=255)
                                                                                                 tataCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGttt                                             <  1:960779/141‑1 (MQ=255)
                                                                                                   taCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTaa                                           >  1:750027/1‑141 (MQ=255)
                                                                                                    aCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATGAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTaaa                                          >  1:932790/1‑141 (MQ=255)
                                                                                                           aaataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAAt                                   >  2:866184/1‑141 (MQ=255)
                                                                                                            aataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATc                                  >  2:448835/1‑141 (MQ=255)
                                                                                                            aataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATc                                  <  1:940474/141‑1 (MQ=255)
                                                                                                             ataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCt                                 >  1:490071/1‑141 (MQ=255)
                                                                                                                   gATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTTAGTTTAAAAAGTAATCTATGTTa                           >  2:477076/1‑141 (MQ=255)
                                                                                                                    aTTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTaa                          <  2:920297/141‑1 (MQ=255)
                                                                                                                      tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTCTGTTAAAt                        >  1:126092/1‑141 (MQ=255)
                                                                                                                      tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAt                        <  2:213236/141‑1 (MQ=255)
                                                                                                                      tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAt                        >  1:456779/1‑141 (MQ=255)
                                                                                                                      tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAt                        >  2:295427/1‑141 (MQ=255)
                                                                                                                      tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGATTAAAAAGTAATCTATGTTAAAt                        <  2:451394/141‑1 (MQ=255)
                                                                                                                       ttATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAtt                       <  1:907418/141‑1 (MQ=255)
                                                                                                                         aTCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTaa                     >  2:242949/1‑141 (MQ=255)
                                                                                                                          tCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAAt                    >  1:357542/1‑141 (MQ=255)
                                                                                                                          tCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAAt                    >  1:576105/1‑141 (MQ=255)
                                                                                                                            tGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATAc                  >  1:719684/1‑141 (MQ=255)
                                                                                                                             gAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATAcc                 <  1:794212/141‑1 (MQ=255)
                                                                                                                              aaTGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCt                >  1:286410/1‑141 (MQ=255)
                                                                                                                                 ggCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGt             >  2:1013773/1‑141 (MQ=255)
                                                                                                                                   cTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTAt           <  1:540064/141‑1 (MQ=255)
                                                                                                                                    tATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTAtt          >  1:634510/1‑141 (MQ=255)
                                                                                                                                      ttCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTaa        >  1:377990/1‑141 (MQ=255)
                                                                                                                                       tCTAAATTAAACGCATTAAAACCAACATCGTACTGAAATTTGACTATAATAATGCATTAGAATTTTATTAACTAGTCAATATTCGTATCTCTGACTTGAGATGAAAAAGTAAACTATGTTAAGTTGACCCCTGGTCTTacg       <  2:381992/141‑3 (MQ=255)
                                                                                                                                        cTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTaaaa      <  1:743552/141‑1 (MQ=255)
                                                                                                                                          aaaTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAAt    <  2:723617/141‑1 (MQ=255)
                                                                                                                                          aaaTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAAt    <  2:723535/141‑1 (MQ=255)
                                                                                                                                          aaaTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAAt    >  2:552095/1‑141 (MQ=255)
                                                                                                                                            aTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACATGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAAttt  <  2:406460/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GTTTCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAAATTT  >  CP000730/984929‑985209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: