Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,049,332 G→A 5.9% R330R (AGG→AGA)  menD → 2‑oxoglutarate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,049,3320GA 5.9% 255.2 / 2.7 83R330R (AGG→AGA) menD2‑oxoglutarate decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (40/38);  new base A (3/2);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CAGTGATTTCTAAAAAGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCGTTGGTGAATTGAT  >  CP000730/1049193‑1049469
                                                                                                                                           |                                                                                                                                         
cAGTGATTTCTAAAAAGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGt                                                                                                                                          >  1:577566/1‑141 (MQ=255)
  gTGATTTCTAAAAAGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCa                                                                                                                                        <  1:454298/141‑1 (MQ=255)
  gTGATTTCTAAAAAGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCa                                                                                                                                        <  2:624104/141‑1 (MQ=255)
  gTGATTTCTAAAAAGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCa                                                                                                                                        <  1:817912/141‑1 (MQ=255)
   tGATTTCTAAAAAGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCAt                                                                                                                                       >  1:774181/1‑141 (MQ=255)
     aTTTCTAAAAAGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTa                                                                                                                                     >  2:910576/1‑141 (MQ=255)
       ttCTAAAAAGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAAt                                                                                                                                   <  2:343641/141‑1 (MQ=255)
           aaaaaGTTGAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCTATAGCGCCAGATATTTCATATGAGATTTCTGCAAATGATTTCTTTAGATCATTAATGGaa                                                                                                                               >  1:230271/1‑141 (MQ=255)
            aaaaGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAg                                                                                                                              <  2:321035/141‑1 (MQ=255)
             aaaGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGa                                                                                                                             <  2:147198/141‑1 (MQ=255)
               aGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGaca                                                                                                                           >  1:967482/1‑141 (MQ=255)
                 ttAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACg                                                                                                                         >  2:691816/1‑141 (MQ=255)
                 ttAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGCCATTAATGGAAGACACg                                                                                                                         >  1:548554/1‑141 (MQ=255)
                  tAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGa                                                                                                                        <  2:44635/141‑1 (MQ=255)
                    aaTCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTATAGGTCATTAATGGAAGACACGACa                                                                                                                      >  1:622617/1‑141 (MQ=255)
                      tCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGt                                                                                                                    >  2:142504/1‑141 (MQ=255)
                      tCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGt                                                                                                                    >  2:622624/1‑141 (MQ=255)
                          tGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAAt                                                                                                                >  2:631663/1‑141 (MQ=255)
                              tAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCTATAGCGCCAGATATTTCATATGAGATTTCTGCAAATGATTTCTTTAGATCATTAATGGAAGACACGAccatcaatcgcg                                                                                                            <  1:107686/141‑12 (MQ=255)
                              tAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATcgcg                                                                                                            >  1:738531/1‑141 (MQ=255)
                                aaGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTa                                                                                                          >  2:516705/1‑141 (MQ=255)
                                    aaaCTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGtt                                                                                                      >  2:696933/1‑141 (MQ=255)
                                       cTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGt                                                                                                   <  2:433388/141‑1 (MQ=255)
                                         gATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTa                                                                                                 <  1:382117/141‑1 (MQ=255)
                                          aTGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAg                                                                                                >  1:183090/1‑141 (MQ=255)
                                            gCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGaa                                                                                              <  2:20408/141‑1 (MQ=255)
                                                 tCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATg                                                                                         >  1:592590/1‑141 (MQ=255)
                                                  cAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATgg                                                                                        <  1:631547/141‑1 (MQ=255)
                                                   agatatTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGc                                                                                       <  1:331264/139‑1 (MQ=255)
                                                   aaaTATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGc                                                                                       <  1:787605/141‑1 (MQ=255)
                                                    aaTATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCa                                                                                      <  2:430973/141‑1 (MQ=255)
                                                     atatTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCaa                                                                                     <  1:180295/141‑1 (MQ=255)
                                                     atatTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCaa                                                                                     <  1:332166/141‑1 (MQ=255)
                                                       atTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATg                                                                                   >  1:1066993/1‑141 (MQ=255)
                                                         tAGTGAAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATTGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCt                                                                                 <  1:705479/141‑1 (MQ=255)
                                                           gTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTa                                                                               >  2:250684/1‑141 (MQ=255)
                                                                aaaCAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGaa                                                                          >  1:611144/1‑141 (MQ=255)
                                                                aaaCAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGaa                                                                          <  1:691816/141‑1 (MQ=255)
                                                                    aaTGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGaaaaaa                                                                      >  1:563907/1‑141 (MQ=255)
                                                                       gATATGTTTGTTGGCTTTCCGAGAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGAGACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAAAGAAAAAAggg                                                                   <  2:406636/141‑1 (MQ=255)
                                                                       gATAAGATTGATGTCTTTCCTATAGCGCCAGATATTTCATATGAGATTTCTGCAAATGATTTCTTTAGATCATTAATGGAAGACACGACCATCAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAggg                                                                   >  1:340086/1‑141 (MQ=255)
                                                                       gATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATTCTTAGAGAAAAAAggg                                                                   >  1:461676/1‑141 (MQ=255)
                                                                         tAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATAGGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCg                                                                 >  1:845406/1‑141 (MQ=255)
                                                                           aGATTGATGTCTTTCCTATCGCGCCAGATATTTCATATGAGATTTCTGCAAATGATTTCTTTAGATCATTAATGGAAGACACGACCATCAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTa                                                               <  2:94790/141‑1 (MQ=255)
                                                                            gATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTaa                                                              >  1:41118/1‑141 (MQ=255)
                                                                             aTTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTaaa                                                             <  1:834258/141‑1 (MQ=255)
                                                                                 aTGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGaaa                                                         >  2:922496/1‑141 (MQ=255)
                                                                                  tGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAAt                                                        >  2:610724/1‑141 (MQ=255)
                                                                                   gTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAAtt                                                       >  1:781/1‑141 (MQ=255)
                                                                                        tCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATg                                                  <  1:301273/141‑1 (MQ=255)
                                                                                          cGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGtt                                                >  1:948138/1‑141 (MQ=255)
                                                                                          cGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGtt                                                >  1:617534/1‑141 (MQ=255)
                                                                                          cGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACAAGAAAGTTAATAGAGTAAGTTGGTTAGCAAGATGGTACTGTTTTGAGAAAAAAGGGCGTAAAGAAATTAAATGtt                                                >  1:864362/1‑141 (MQ=255)
                                                                                               gcgcCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTg                                           >  1:5490/1‑141 (MQ=255)
                                                                                               gcgcCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAAGGCTTAGCGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTg                                           >  2:983090/1‑141 (MQ=255)
                                                                                                cgcCAGATATTTCANATGAGATTTCTGCGAANGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTNAGAGAAAANAGGGCGTAAAGAAATTAAATGTTATTTgg                                          <  1:261424/141‑1 (MQ=255)
                                                                                                    agttatTTTATATGAGGTTTCTGCGAATGGTTTATTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTGGGTTAGAAAAATGTCAAGGCTTAGCGAAAAAAGGGCGTAAAGATATTAAATGTTTTTTGGTACa                                      <  2:475942/138‑1 (MQ=255)
                                                                                                      atatTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAg                                    <  2:758340/141‑1 (MQ=255)
                                                                                                        atTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCt                                  >  1:610037/1‑141 (MQ=255)
                                                                                                         tttCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTa                                 <  2:620557/141‑1 (MQ=255)
                                                                                                         tttCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTACCGCAACCAGGACATTTCGCAATTAAATGTTATTTGGAACAAGCTa                                 <  2:864580/141‑1 (MQ=255)
                                                                                                           tCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACa                               <  1:897086/141‑1 (MQ=255)
                                                                                                           tCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACa                               <  2:449969/141‑1 (MQ=255)
                                                                                                           tCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACa                               <  2:114315/141‑1 (MQ=255)
                                                                                                           tCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGGTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACa                               >  1:70226/1‑141 (MQ=255)
                                                                                                            cATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAg                              >  1:165371/1‑141 (MQ=255)
                                                                                                             atatGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTCAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGa                             >  1:739859/1‑141 (MQ=255)
                                                                                                                tGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATga                          >  2:72817/1‑141 (MQ=255)
                                                                                                                   gaTTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGagag                       >  2:870859/1‑141 (MQ=255)
                                                                                                                     tttCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTGAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTg                     <  2:548554/141‑1 (MQ=255)
                                                                                                                       tCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCa                   <  1:1046204/141‑1 (MQ=255)
                                                                                                                       tCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCa                   <  2:363050/141‑1 (MQ=255)
                                                                                                                        cTGCAAATGATTTCTTTAGATCATTAATGGAAGACACGACCATCAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCAt                  >  2:524855/1‑141 (MQ=255)
                                                                                                                          gCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTc                >  2:311710/1‑141 (MQ=255)
                                                                                                                           cGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCg               <  1:1077310/141‑1 (MQ=255)
                                                                                                                           cGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCg               <  1:516705/141‑1 (MQ=255)
                                                                                                                           cGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCg               <  1:143356/141‑1 (MQ=255)
                                                                                                                            gAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCGt              >  1:770948/1‑141 (MQ=255)
                                                                                                                               tGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCGTTgg           <  1:830741/141‑1 (MQ=255)
                                                                                                                                  tttCTTTAGGTCATTAATGGAAGACACGACAGTTAAACGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCGTTGGTGa        >  1:871290/1‑141 (MQ=255)
                                                                                                                                      tttAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCGTTGGTGAATTg    >  2:460742/1‑141 (MQ=255)
                                                                                                                                       ttAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGAAAAAAAAAAAAAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCGTTGGTGAATTGa   <  2:251628/141‑1 (MQ=255)
                                                                                                                                        tAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCGTTGGTGAATTGAt  <  1:839027/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CAGTGATTTCTAAAAAGTTAAATCAATGGTTAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCGATAGCGCCAGATATTTCATATGAGATTTCTGCGAATGATTTCTTTAGGTCATTAATGGAAGACACGACAGTTAATCGCGTAAGTTGGTTAGAAAAATGGCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCGTTGGTGAATTGAT  >  CP000730/1049193‑1049469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑