Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,166,243 A→T 5.5% intergenic (‑537/‑135) USA300HOU_1092 ← / → USA300HOU_1093 hypothetical protein/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,166,2430AT 5.5% 217.1 / 2.1 70intergenic (‑537/‑135)USA300HOU_1092/USA300HOU_1093hypothetical protein/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (34/32);  new base T (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TTTTACTTATAAAGAAATAAGAGATATATTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTGAGGTCAAACGATGAT  >  CP000730/1166107‑1166382
                                                                                                                                        |                                                                                                                                           
ttttACTTATAAAGAAATAAGAGATATATTCTAATTATGGTTTATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGa                                                                                                                                         <  1:39624/141‑1 (MQ=255)
ttttACTTATAAAGAAATAAGAGATATATTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGa                                                                                                                                         <  2:685685/141‑1 (MQ=255)
ttttACTTATAAAGAAATAAGAGATATATTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGa                                                                                                                                         <  2:645994/141‑1 (MQ=255)
ttttACTTATAAAGAAATAAGAGATATATTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGa                                                                                                                                         <  1:230700/141‑1 (MQ=255)
    aCTTATAAAGAAATAAGAGATATATTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATaaa                                                                                                                                     >  1:224994/1‑141 (MQ=255)
              aaaTAAGAGATATATTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTaaa                                                                                                                           <  2:907017/141‑1 (MQ=255)
                      gaTATATTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTg                                                                                                                   <  1:795216/141‑1 (MQ=255)
                       atatatTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAATTAGATAAAATAGTGTAAAATCACGTGc                                                                                                                  <  1:251444/141‑1 (MQ=255)
                         atatTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCa                                                                                                                <  2:808621/141‑1 (MQ=255)
                           atTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAg                                                                                                              >  1:888214/1‑141 (MQ=255)
                             tCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGAt                                                                                                            <  2:234243/141‑1 (MQ=255)
                             tCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGAt                                                                                                            <  1:404400/141‑1 (MQ=255)
                              cTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGAtt                                                                                                           >  2:46730/1‑141 (MQ=255)
                                aaTTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTAc                                                                                                         >  1:589857/1‑141 (MQ=255)
                                     tGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATag                                                                                                    >  1:957199/1‑141 (MQ=255)
                                      ggTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATaga                                                                                                   <  1:347231/141‑1 (MQ=255)
                                        ttGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGt                                                                                                 <  1:964975/141‑1 (MQ=255)
                                             atGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACCTACTTTTTGAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCa                                                                                            <  1:20429/141‑1 (MQ=255)
                                                     aTTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGtat                                                                                    <  1:695315/141‑1 (MQ=255)
                                                      ttAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGtatt                                                                                   <  1:439478/141‑1 (MQ=255)
                                                         atatatAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAAATTACTATAGAGTAGGCATTGAGTattatt                                                                                >  1:620054/1‑141 (MQ=255)
                                                              taAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCaa                                                                           >  2:5931/1‑141 (MQ=255)
                                                                   tAAAAATTATACTATTTAATTGAATTATGCAGTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACt                                                                      <  2:683162/141‑1 (MQ=255)
                                                                    aaaaaTTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACtt                                                                     <  2:148885/141‑1 (MQ=255)
                                                                      aaaTTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTaa                                                                   >  1:141561/1‑141 (MQ=255)
                                                                       aaTTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAg                                                                  >  1:688619/1‑141 (MQ=255)
                                                                           ataCTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGAt                                                              >  1:164792/1‑141 (MQ=255)
                                                                           ataCTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATCATTGGCAATCACTTAAGTGAt                                                              >  2:703684/1‑141 (MQ=255)
                                                                             aCTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTg                                                            <  2:634741/141‑1 (MQ=255)
                                                                                  ttAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTAtt                                                       <  2:411899/141‑1 (MQ=255)
                                                                                  ttAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTAtt                                                       <  2:611820/141‑1 (MQ=255)
                                                                                   tAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTAttt                                                      <  2:454225/141‑1 (MQ=255)
                                                                                     aTTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTAttttt                                                    <  1:718826/141‑1 (MQ=255)
                                                                                        gAATTATGCATTTTCGAAGTAAACATAATTCCACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGt                                                 >  1:8603/1‑141 (MQ=255)
                                                                                        gAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGt                                                 <  2:161031/141‑1 (MQ=255)
                                                                                            tATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTc                                             <  2:692628/141‑1 (MQ=255)
                                                                                            tATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTc                                             <  1:475915/141‑1 (MQ=255)
                                                                                            tATGCATTTCTGAAATAAAAATAATTCAACACATACATTTTTAAAGAGAAAAAATAGTGGAAAATCACGGGACAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGAGACTGTTATTTTCCGTGGTc                                             <  2:300942/141‑1 (MQ=255)
                                                                                             aTGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTAATATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCa                                            >  2:714494/1‑141 (MQ=255)
                                                                                               gCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCaat                                          >  1:562501/1‑141 (MQ=255)
                                                                                               gCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCaat                                          >  1:627105/1‑141 (MQ=255)
                                                                                               gCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCaat                                          >  2:489136/1‑141 (MQ=255)
                                                                                               gCATTTTCGAAGTAAACATAATTCAACAATTAAATTTTTAAATAGATAAAATAATGTAAAATCACGTGACAAGATTACTATAGAGTAGACATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTAGTGGTTaat                                          >  2:792086/1‑141 (MQ=255)
                                                                                                  ttttCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCaataat                                       >  1:1049598/1‑141 (MQ=255)
                                                                                                   tttCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAataata                                      <  1:750785/141‑1 (MQ=255)
                                                                                                    ttCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAAtaataa                                     <  2:637250/141‑1 (MQ=255)
                                                                                                       gAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGa                                  >  2:222870/1‑141 (MQ=255)
                                                                                                        aaGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGat                                 >  1:784010/1‑141 (MQ=255)
                                                                                                         aGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCTAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGata                                >  2:301391/1‑141 (MQ=255)
                                                                                                          gTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGatat                               >  1:413540/1‑141 (MQ=255)
                                                                                                              aCATAATTCAACACTTACATTTTTAAATAGATAATATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATataaat                           <  1:1004871/141‑1 (MQ=255)
                                                                                                                 tAATTCAACACTTACATTTTTTTTTTTTTTTAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATAtaaataaa                        >  2:443394/1‑141 (MQ=255)
                                                                                                                 tAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGCTTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACGTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATAtaaataaa                        >  1:757613/1‑141 (MQ=255)
                                                                                                                 tAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATAtaaataaa                        <  2:1076495/141‑1 (MQ=255)
                                                                                                                  aaTTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATaaataaat                       <  2:380807/141‑1 (MQ=255)
                                                                                                                   aTTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATACTAAAGATATAaataaata                      <  2:592295/141‑1 (MQ=255)
                                                                                                                   aTTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAaataaata                      >  2:262261/1‑141 (MQ=255)
                                                                                                                   aTTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAaataaata                      >  1:506056/1‑141 (MQ=255)
                                                                                                                   aTTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAaataaata                      >  1:181223/1‑141 (MQ=255)
                                                                                                                      cAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAatatat                   >  2:294921/1‑141 (MQ=255)
                                                                                                                      cAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAatatat                   >  1:172018/1‑141 (MQ=255)
                                                                                                                      cAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCAAGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAatatat                   >  2:652513/1‑141 (MQ=255)
                                                                                                                        acacTTACATTTTTAATTAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTg                 >  2:797651/1‑141 (MQ=255)
                                                                                                                            ttACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTGAGGt             >  2:329329/1‑141 (MQ=255)
                                                                                                                            ttACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTAATATTGGCAATCACTTAAGTGATTGCTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTGAGGt             >  1:581754/1‑141 (MQ=255)
                                                                                                                              aCATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTGAGGTCa           <  1:492235/141‑1 (MQ=255)
                                                                                                                              aCATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTGAGGTCa           >  1:170571/1‑141 (MQ=255)
                                                                                                                               cATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTGAGGTCaa          >  2:202726/1‑141 (MQ=255)
                                                                                                                                aTTTTTAATTAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTGAGGTCaaa         <  2:679042/141‑1 (MQ=255)
                                                                                                                                 tttttAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTGAGGTCAAAc        >  2:82138/1‑141 (MQ=255)
                                                                                                                                       aaTAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTGAGGTCAAACgatgat  >  2:565817/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                           
TTTTACTTATAAAGAAATAAGAGATATATTCTAATTATGGTTGATATGTTATGATTAATATATAAGTTAAAAATTATACTATTTAATTGAATTATGCATTTTCGAAGTAAACATAATTCAACACTTACATTTTTAAATAGATAAAATAGTGTAAAATCACGTGCCAAGATTACTATAGAGTAGGCATTGAGTATTATTGGCAATCACTTAAGTGATTGTTATTTTTCGTGGTCAATAATAAAGATATAAATAAATATATTGAGGTCAAACGATGAT  >  CP000730/1166107‑1166382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑