Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 9,710 T→C 9.1% intergenic (+15/+72) gyrA → / ← nnrD DNA topoisomerase (ATP‑hydrolyzing) subunit A/possible carbohydrate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007309,7100TC 9.1% 308.3 / 16.3 119intergenic (+15/+72)gyrA/nnrDDNA topoisomerase (ATP‑hydrolyzing) subunit A/possible carbohydrate kinase
Reads supporting (aligned to +/- strand):  ref base T (51/55);  new base C (3/8);  total (55/64)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 122 total aligned reads displayed.

AATGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACAT  >  CP000730/9573‑9850
                                                                                                                                          |                                                                                                                                            
aaTGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTccc                                                                                                                                           >  1:686842/1‑141 (MQ=255)
  tGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCta                                                                                                                                         <  2:850443/141‑1 (MQ=255)
  tGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCta                                                                                                                                         <  2:297164/141‑1 (MQ=255)
    cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGCGGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAACATATACAACAATACTCAGATGAAGAATAATAAAAAATAAGACTTCCCtata                                                                                                                                       <  2:186284/141‑1 (MQ=255)
    cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCtata                                                                                                                                       <  2:186298/141‑1 (MQ=255)
    cAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTCCCCtaca                                                                                                                                       <  2:338472/141‑3 (MQ=255)
    cAATGCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCtata                                                                                                                                       <  2:552855/141‑1 (MQ=255)
     aaTTCATACTGAAGTGATTGATTCAGAAGAAAATGATGACGATGGACGTATTGAAGTAAGACAAGATTTCATGGCTCGTGGTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTatat                                                                                                                                      >  2:908101/1‑141 (MQ=255)
     aaTTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTatat                                                                                                                                      >  1:688030/1‑141 (MQ=255)
       ttCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGt                                                                                                                                    <  2:476128/141‑1 (MQ=255)
       ctgaagtgattgattcagaagaaaatgatgaagatggacgtattgaagtaagacaagatttcatggatcgtgttgaagaagatatacaacaatcatcagatgaAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATg                                                                                                                                     >  2:1061339‑M2/104‑141 (MQ=255)
            aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAgggg                                                                                                                               >  1:997634/1‑141 (MQ=255)
            aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAggga                                                                                                                               <  1:113654/141‑2 (MQ=255)
             cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATTGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGa                                                                                                                              <  2:296796/141‑1 (MQ=255)
               gAAGTGAGTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGt                                                                                                                            <  2:224369/141‑1 (MQ=255)
                 aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCt                                                                                                                          <  1:432935/141‑1 (MQ=255)
                 aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCt                                                                                                                          <  1:433007/141‑1 (MQ=255)
                 aGTGATGGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCt                                                                                                                          <  2:837236/141‑1 (MQ=255)
                  gTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCtt                                                                                                                         <  1:211969/141‑1 (MQ=255)
                   tgattgatTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTAt                                                                                                                       <  1:107740/141‑1 (MQ=255)
                        gattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTAttttt                                                                                                                   <  2:283433/141‑1 (MQ=255)
                         attCATAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTa                                                                                                                  <  2:970307/141‑1 (MQ=255)
                          ttCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTAt                                                                                                                 >  1:177309/1‑141 (MQ=255)
                             agaagaAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCTTAttttttatta                                                                                                              <  1:618374/141‑6 (MQ=38)
                               aagaaAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGCGATACAACAATCATCAGATGAAGAATAATAAAAAAAAAGACTCCTCTCTAGGTCGCGGAGCCTTATTCTTATGCTAg                                                                                                            >  1:562203/1‑141 (MQ=255)
                                  aaaaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAGGAATAATAAAAAATAAGACTTCCCTACATGTAGGGGAGTCTTATTATTAAGCTAGaaa                                                                                                         >  2:794063/1‑141 (MQ=255)
                                    aaTGATGAAGATGGACGTATGGAAGTAAGACAAGATTTCATGGATCGTATTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGGAAGTCTTAttttttattattcttca                                                                                                       <  2:74018/141‑13 (MQ=25)
                                    aaTGATGAAGATGGACGTATAGAAGTAAAACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTCCCCTACATATAGGAAAGTCTTAttttttattattcttca                                                                                                       <  2:345455/141‑13 (MQ=25)
                                    aaTGATGAAGATGGACGTAAGTAATTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGt                                                                                                       <  1:283622/141‑1 (MQ=255)
                                      tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                     <  1:210884/141‑1 (MQ=255)
                                      tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                     <  2:990334/141‑1 (MQ=255)
                                      tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                     <  1:622781/141‑1 (MQ=255)
                                      tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATAAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTaa                                                                                                     >  1:243254/1‑141 (MQ=255)
                                              aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGCAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTAc                                                                                             <  1:103409/141‑1 (MQ=255)
                                              aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTAc                                                                                             <  2:199616/141‑1 (MQ=255)
                                              aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTAc                                                                                             <  1:210719/141‑1 (MQ=255)
                                                     tATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATAAGTAGGGGAGGCTTATTTCTATGCTAGAAAGTAATGCCTTACTATATTc                                                                                      >  2:954296/1‑141 (MQ=255)
                                                        tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt                                                                                   <  2:293606/141‑1 (MQ=255)
                                                            gTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTCTTATGCTAGAAAGTAATGCTTTACTATATTCAATGAtt                                                                               >  2:489259/1‑141 (MQ=255)
                                                            caagatttcatggatcgtgttgaagaagatatacaacaatcatcagatgaAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTGTACTATATTCAATGAt                                                                                <  1:451709‑M2/91‑1 (MQ=255)
                                                               aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt                                                                            >  2:455014/1‑141 (MQ=255)
                                                               aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt                                                                            <  1:564714/141‑1 (MQ=255)
                                                                gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa                                                                           >  1:100784/1‑141 (MQ=255)
                                                                    aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                       <  1:521773/141‑1 (MQ=255)
                                                                    aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                       <  2:940476/141‑1 (MQ=255)
                                                                     gATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGAc                                                                      <  1:90786/141‑1 (MQ=255)
                                                                      aTTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGNTTTACTATATTCNATGATTAGTAATGACt                                                                     <  1:657834/141‑1 (MQ=255)
                                                                         tCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAAc                                                                  >  1:134828/1‑141 (MQ=255)
                                                                            tGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                               >  2:20686/1‑141 (MQ=255)
                                                                            tGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                               >  2:181790/1‑141 (MQ=255)
                                                                            tGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                               >  1:177473/1‑141 (MQ=255)
                                                                              gATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                             >  1:1030712/1‑141 (MQ=255)
                                                                              gATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                             <  2:321510/141‑1 (MQ=255)
                                                                               aTCGTGTTGAAGAAGATATACAACAATCATGAGATGGAGAATAATAAAAAATAAGACTACCCTATATGAAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTa                                                            >  2:913163/1‑141 (MQ=255)
                                                                                  gtgtTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt                                                         <  1:709536/141‑1 (MQ=255)
                                                                                  gtgtTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt                                                         >  1:127116/1‑141 (MQ=255)
                                                                                  gtgtTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAtt                                                         <  1:671668/141‑1 (MQ=255)
                                                                                   tgtTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCNATGATTAGTAATNACTAACTTTCTAATTg                                                        <  1:876416/141‑1 (MQ=255)
                                                                                    gtTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGt                                                       >  2:457960/1‑141 (MQ=255)
                                                                                      tGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGttt                                                     >  1:489802/1‑141 (MQ=255)
                                                                                         agaagaTATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCAt                                                  >  2:683224/1‑141 (MQ=255)
                                                                                             gaTATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCg                                              <  2:686842/141‑1 (MQ=255)
                                                                                               tataCAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTa                                            >  2:663054/1‑141 (MQ=255)
                                                                                                  acaacaATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                         <  1:271121/141‑1 (MQ=255)
                                                                                                  acaacaATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                         <  2:185885/141‑1 (MQ=255)
                                                                                                  acaacaATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                         <  2:892026/141‑1 (MQ=255)
                                                                                                  acaacaATCATCAGATGAAGAATAATAAAAAACAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                         <  1:1041746/141‑1 (MQ=255)
                                                                                                   caacaaTCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                        >  2:393441/1‑141 (MQ=255)
                                                                                                   caacaaTCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCCTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                        >  2:499878/1‑141 (MQ=255)
                                                                                                     acaaTCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt                                      <  2:344300/141‑1 (MQ=255)
                                                                                                     acaaTCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt                                      <  1:833678/141‑1 (MQ=255)
                                                                                                       aaTCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAttt                                    <  1:332201/141‑1 (MQ=255)
                                                                                                         tcatcaGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                  >  2:724733/1‑141 (MQ=255)
                                                                                                         tcatcaGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                  >  2:666732/1‑141 (MQ=255)
                                                                                                         tcatcaGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                  <  2:1026836/141‑1 (MQ=255)
                                                                                                             caGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa                              >  2:736408/1‑141 (MQ=255)
                                                                                                             caGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa                              >  2:907586/1‑141 (MQ=255)
                                                                                                              aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                             >  2:125287/1‑141 (MQ=255)
                                                                                                              aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                             <  2:1010903/141‑1 (MQ=255)
                                                                                                              aGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                             <  1:323896/141‑1 (MQ=255)
                                                                                                                    agaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGCGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAATTTTCTAATTGTTTCGTTGCGTAAGGTATTTCATTGATGAGTCt                       >  2:548700/1‑141 (MQ=255)
                                                                                                                    agaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCt                       <  1:840406/141‑1 (MQ=255)
                                                                                                                    agaaTAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCt                       <  2:389844/141‑1 (MQ=255)
                                                                                                                         aataaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCTTTGCGTAAGGTATTTCATTGATAGGTCTTGAtg                  >  1:682025/1‑141 (MQ=255)
                                                                                                                          ataaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg                 <  2:717268/141‑1 (MQ=255)
                                                                                                                          ataaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg                 >  1:728038/1‑141 (MQ=255)
                                                                                                                          ataaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg                 >  1:728157/1‑141 (MQ=255)
                                                                                                                             aaaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtg               >  2:576217/1‑141 (MQ=255)
                                                                                                                              aaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtgg              <  2:194244/141‑1 (MQ=255)
                                                                                                                                aaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGca            <  2:761296/141‑1 (MQ=255)
                                                                                                                                aaaTAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTAATGGTTGca            >  2:890190/1‑141 (MQ=255)
                                                                                                                                     aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCacaaca       >  2:804462/1‑141 (MQ=255)
                                                                                                                                     aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca       >  1:520281/1‑141 (MQ=255)
                                                                                                                                     aGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca       >  1:244719/1‑141 (MQ=255)
                                                                                                                                     aGACTCCCCTACATATAGGGAAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca       >  1:451741/1‑141 (MQ=255)
                                                                                                                                      gACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacat      >  2:455634/1‑141 (MQ=255)
                                                                                                                                        cTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatac    <  1:196416/141‑1 (MQ=255)
                                                                                                                                          tCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat  >  1:698677/1‑141 (MQ=255)
                                                                                                                                          tCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat  >  1:815935/1‑141 (MQ=255)
                                                                                                                                          tCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat  >  1:256462/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
AATGCAATTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGAATAATAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTATTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACAT  >  CP000730/9573‑9850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: