Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,299,755	T→A	9.1%	V472V (GTT→GTA)	rnjB →	beta‑lactamase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,299,755	0	T	A	9.1%	230.0 / 12.3	78	V472V (GTT→GTA)	rnjB	beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base T (36/34);  new base A (5/2);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

ACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGTTTAC  >  CP000730/1299616‑1299894                                                                                                                                            |                                                                                                                                            aCAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGATCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCtgttg                                                                                                                                            >  2:826742/1‑141 (MQ=255)  cAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCtgttgt                                                                                                                                           <  1:697861/141‑1 (MQ=255)   aaCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTa                                                                                                                                          >  1:372266/1‑141 (MQ=255)   aaCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTa                                                                                                                                          >  2:877971/1‑141 (MQ=255)    aCGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTaa                                                                                                                                         >  2:90156/1‑141 (MQ=255)      ggTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACg                                                                                                                                       <  2:302163/141‑1 (MQ=255)       gTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGt                                                                                                                                      <  2:781333/141‑1 (MQ=255)        tAAAGATATGATATTAAATGAAAAGGTAAATTCGGGAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGtt                                                                                                                                     <  1:695134/141‑1 (MQ=255)        tAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGtt                                                                                                                                     <  2:212844/141‑1 (MQ=255)           aGATATGATATTAAATGAAAAGGTAAATTCGGGAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAAGAGCGTGTGGATAGACCGGCATCTTTGAGCAGAAGATGGTATCGTTATTGCTGTAGTAACGTTAGa                                                                                                                                  >  1:918826/1‑141 (MQ=255)            gatatgatatTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAt                                                                                                                                 >  1:204329/1‑141 (MQ=255)            gatatgatatTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAt                                                                                                                                 >  2:567149/1‑141 (MQ=255)             atatgatatTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATc                                                                                                                                <  2:259488/141‑1 (MQ=255)                     ttAAATGAAAAGGTAAATTCGGGAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGTTAGATCCTAAAAAt                                                                                                                        >  2:53627/1‑141 (MQ=255)                        aaTGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGa                                                                                                                     >  1:467402/1‑141 (MQ=255)                        aaTGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGa                                                                                                                     >  2:718847/1‑141 (MQ=255)                               aGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAg                                                                                                              >  2:836729/1‑141 (MQ=255)                                ggTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGc                                                                                                             >  1:922623/1‑141 (MQ=255)                                    aaTTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCg                                                                                                         >  1:531378/1‑141 (MQ=255)                                     aTTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCgg                                                                                                        >  1:120470/1‑141 (MQ=255)                                        cgggAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCgggtc                                                                                                     >  1:380776/3‑139 (MQ=255)                                        cgggAAATATTTTAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCgggtc                                                                                                     >  1:380867/3‑139 (MQ=255)                                        cAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAACCTAAAAATAGACGTATAGCTGCGGGAc                                                                                                     <  2:1085153/141‑1 (MQ=255)                                          ggAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGGTGGTTATTTTGTTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCt                                                                                                   >  1:613788/1‑141 (MQ=255)                                          ggAAATATTTTAATAGATGGCATTGGTATTAGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCGTAAAAATCTACGTATAGCTGCGGGACCt                                                                                                   >  2:728062/1‑141 (MQ=255)                                             aaTATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGaa                                                                                                >  2:4802/1‑141 (MQ=255)                                               tatTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAAt                                                                                              >  2:635619/1‑141 (MQ=255)                                                atTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAAtt                                                                                             >  1:276939/1‑141 (MQ=255)                                                  tttAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCa                                                                                           <  2:922623/141‑1 (MQ=255)                                                   ttAATAGATGGTATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGTCCTGAAATTCaa                                                                                          >  2:84750/1‑141 (MQ=255)                                                   ttAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCaa                                                                                          >  1:379062/1‑141 (MQ=255)                                                    tAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAAt                                                                                         >  1:809542/1‑141 (MQ=255)                                                      aTAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAAtct                                                                                       >  1:295118/1‑141 (MQ=255)                                                      aTAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAAtct                                                                                       >  1:1014618/1‑141 (MQ=255)                                                      aTAGATGGCAGTGGTATGGGGGATGTAGGAAGTATCGTGTGGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAAtct                                                                                       <  2:990595/141‑1 (MQ=255)                                                        aGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCg                                                                                     <  2:888301/141‑1 (MQ=255)                                                          aTGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTg                                                                                   <  1:54198/141‑1 (MQ=255)                                                                ttGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTg                                                                             <  2:71874/141‑1 (MQ=255)                                                                ttGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTg                                                                             <  1:77516/141‑1 (MQ=255)                                                                      ttGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATg                                                                       <  2:159481/141‑1 (MQ=255)                                                                       tGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGt                                                                      <  1:462644/141‑1 (MQ=255)                                                                         gggATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTAc                                                                    <  1:183445/141‑1 (MQ=255)                                                                          ggATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGAGGGTATCTTTAGTGCTGTTGTAACGTTAGATCCTAAAAATAGTCGTATAGCTGCGGGACCTGGAATTCAATCGCGTGGGTTTGTATATGTACg                                                                   <  2:976211/141‑1 (MQ=255)                                                                           gATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGt                                                                  <  1:90156/141‑1 (MQ=255)                                                                            aTGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTg                                                                 >  2:622352/1‑141 (MQ=255)                                                                             tGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGa                                                                <  2:818513/141‑1 (MQ=255)                                                                              gTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGaa                                                               <  1:73612/141‑1 (MQ=255)                                                                                aGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAg                                                             >  1:832792/1‑141 (MQ=255)                                                                                       atCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTCTTGCTGACGTGACAACAGATCCTAATAATAAACGTATAGCTGCGGTACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGAc                                                      <  1:758328/141‑1 (MQ=255)                                                                                       atCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGAc                                                      <  1:1004346/141‑1 (MQ=255)                                                                                         cGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGAAACTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACtt                                                    <  2:301335/141‑1 (MQ=255)                                                                                          gtgtTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACtta                                                   >  2:323607/1‑141 (MQ=255)                                                                                          gtgtTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACtta                                                   >  2:637337/1‑141 (MQ=255)                                                                                           tgtTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACttat                                                  <  1:306588/141‑1 (MQ=255)                                                                                            tttGAGAGACCGTCATCTTTTAGCAGAAGATGGTAGCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACttatt                                                 <  1:455941/140‑1 (MQ=255)                                                                                                 gagaCCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTg                                            >  2:88752/1‑141 (MQ=255)                                                                                                   gaCCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGaa                                          >  2:1063615/1‑141 (MQ=255)                                                                                                    aCCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAg                                         <  1:675611/141‑1 (MQ=255)                                                                                                     ccGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGc                                        >  1:764430/1‑141 (MQ=255)                                                                                                      cGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCa                                       <  1:821044/141‑1 (MQ=255)                                                                                                          aTCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCagaag                                   <  2:479044/141‑1 (MQ=255)                                                                                                            cTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAagag                                 <  1:561750/141‑1 (MQ=255)                                                                                                             ttttAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGCCCTGAAATTGAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAgaga                                >  1:1028049/1‑141 (MQ=255)                                                                                                             ttttAGCAGAAGATGGTATCTGTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAgaga                                <  2:702147/141‑1 (MQ=255)                                                                                                             ttctgctaaaagaTGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAgaga                                >  2:974607/10‑141 (MQ=255)                                                                                                             ttctgctaaaagaTGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAgaga                                >  2:581850/10‑141 (MQ=255)                                                                                                               ttAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTACAGCCGCGGGACGTGAAAACCAATCTCGGGGGTTTTAATATGTACGTGAAAGTGAAGTCTGATGACGTGAAGCAGAAGAGaaa                              >  1:918574/1‑141 (MQ=255)                                                                                                               ctgctaaaagaTGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGaaa                              >  1:114497/8‑141 (MQ=255)                                                                                                                tAGCAGAAGATGGTATCTTTATTGCTGTAGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGTCCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAg                             <  1:45994/141‑1 (MQ=255)                                                                                                                  gCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTa                           <  2:1074644/141‑1 (MQ=255)                                                                                                                      tataTGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTg                       <  2:283193/138‑1 (MQ=255)                                                                                                                      aagaTGGTATCTTTATTGCTGTTGTAAAAATAGAAAATAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTg                       <  2:283324/141‑1 (MQ=255)                                                                                                                           ggTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAAta                  >  1:309806/1‑141 (MQ=255)                                                                                                                            gTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAAtag                 >  1:697139/1‑141 (MQ=255)                                                                                                                            gTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAAtag                 <  1:850744/141‑1 (MQ=255)                                                                                                                              aTCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAAtagta               >  1:86190/1‑141 (MQ=255)                                                                                                                                cTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTaga             <  2:543846/141‑1 (MQ=255)                                                                                                                                cTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTaga             <  2:1034400/141‑1 (MQ=255)                                                                                                                                 tttATTGCTGTTGTAAGGTTAGTTCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGTGTTTGTATTTGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTagag            <  1:454113/141‑1 (MQ=255)                                                                                                                                        cTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGtt     >  1:1058143/1‑141 (MQ=255)                                                                                                                                          gttgtAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGTTTa   >  1:920028/1‑141 (MQ=255)                                                                                                                                           ttgtAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGTTTAc  >  1:476807/1‑141 (MQ=255)                                                                                                                                            |                                                                                                                                            ACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGTTTAC  >  CP000730/1299616‑1299894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑