Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,341,933 G→A 6.1% intergenic (+341/‑171) USA300HOU_1245 → / → USA300HOU_1246 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,341,9330GA 6.1% 248.2 / 4.1 82intergenic (+341/‑171)USA300HOU_1245/USA300HOU_1246hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/41);  new base A (3/2);  total (39/43)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

AAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATA  >  CP000730/1341798‑1342070
                                                                                                                                       |                                                                                                                                         
aaaaGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGa                                                                                                                                      <  2:622039/141‑1 (MQ=255)
 aaaGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAg                                                                                                                                     <  1:893794/141‑1 (MQ=255)
   aGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTg                                                                                                                                   >  1:902788/1‑141 (MQ=255)
   aGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTg                                                                                                                                   <  1:238312/141‑1 (MQ=255)
        ggAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGa                                                                                                                              >  1:288414/1‑141 (MQ=255)
        ggAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGa                                                                                                                              <  1:244204/141‑1 (MQ=255)
              gATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATaaa                                                                                                                        >  2:427820/1‑141 (MQ=255)
                  gTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAg                                                                                                                    <  2:135506/141‑1 (MQ=255)
                          aTGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATg                                                                                                            <  2:1065953/141‑1 (MQ=255)
                          aTGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATg                                                                                                            <  1:444294/141‑1 (MQ=255)
                            gCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTa                                                                                                          >  1:87425/1‑141 (MQ=255)
                             cTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTAc                                                                                                         >  1:693775/1‑141 (MQ=255)
                                 cTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCaaa                                                                                                     <  2:424719/141‑1 (MQ=255)
                                  ttCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAAt                                                                                                    >  1:941329/1‑141 (MQ=255)
                                  ataaaaGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGAAAAAAAAAAAAAAAAGCTCAAATGTACCAAAt                                                                                                    <  2:754648/138‑1 (MQ=255)
                                      aaGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGtt                                                                                                <  1:330067/141‑1 (MQ=255)
                                         ttttCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGCTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCCCAAATGTACCAAATTGTTAAt                                                                                             >  1:1060926/1‑141 (MQ=255)
                                           ttCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCt                                                                                           >  1:1003280/1‑141 (MQ=255)
                                           ttCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCt                                                                                           >  1:1056057/1‑141 (MQ=255)
                                            tCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCtt                                                                                          >  1:533733/1‑141 (MQ=255)
                                              aTTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaa                                                                                        >  1:630735/1‑141 (MQ=255)
                                               ttttttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaat                                                                                       >  2:875874/1‑141 (MQ=255)
                                                tttttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaata                                                                                      <  1:538264/141‑1 (MQ=255)
                                                tttttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATCGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTtaata                                                                                      >  2:934058/1‑141 (MQ=255)
                                                   ttttttGTCGACAACGCAGGCGGCATTTTCATTAAATTGTTATAGCTTTTTATCTTTGTATAATGAACATATTATTTTTATTTTTTTCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAAt                                                                                   >  2:1080651/6‑141 (MQ=255)
                                                   ttCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAAt                                                                                   <  2:566413/141‑1 (MQ=255)
                                                    tCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAAtc                                                                                  >  2:1084416/1‑141 (MQ=255)
                                                        tGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTa                                                                              >  1:658064/1‑141 (MQ=255)
                                                         gtcgtcTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTAc                                                                             <  2:753710/141‑1 (MQ=255)
                                                         gTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTAc                                                                             <  1:207381/141‑1 (MQ=255)
                                                           cGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACtt                                                                           <  1:885126/141‑1 (MQ=255)
                                                            gtgttCGAAGGGGGCATTTTCATTAAATTGTTGTAGGTGTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGTAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACttt                                                                          <  2:942859/138‑1 (MQ=255)
                                                              cTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtat                                                                        >  1:352606/1‑141 (MQ=255)
                                                               ttCGAAGGGGGCGTTTTCATTAAATTGTTATAGCTTTTTATATTTGTATGATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtata                                                                       <  2:649145/141‑1 (MQ=255)
                                                               ttCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtata                                                                       <  1:328185/141‑1 (MQ=255)
                                                               ttCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTtata                                                                       >  1:185597/1‑141 (MQ=255)
                                                                tCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATaa                                                                      <  2:236199/141‑1 (MQ=255)
                                                                tCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATaa                                                                      <  2:147156/141‑1 (MQ=255)
                                                                 cGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATaaa                                                                     <  2:45979/141‑1 (MQ=255)
                                                                    aGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGAt                                                                  <  1:454950/141‑1 (MQ=255)
                                                                     gggggCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGAtt                                                                 >  1:872028/1‑141 (MQ=255)
                                                                      ggggCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTg                                                                >  1:1065926/1‑141 (MQ=255)
                                                                      ggggCATTTCCATTATATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTg                                                                >  2:985215/1‑141 (MQ=255)
                                                                             tttCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGAc                                                         >  2:212326/1‑141 (MQ=255)
                                                                              ttCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACa                                                        <  1:81477/141‑1 (MQ=255)
                                                                                  ttataTTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCg                                                    >  1:85716/5‑141 (MQ=255)
                                                                                  ttAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCg                                                    <  1:646146/141‑1 (MQ=255)
                                                                                   tAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGAGAAAGAAAAAAAAAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGa                                                   <  2:134415/141‑1 (MQ=255)
                                                                                    ataTTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAg                                                  <  2:483140/139‑1 (MQ=255)
                                                                                          ttATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTaa                                            >  2:625314/1‑141 (MQ=255)
                                                                                          ttATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTaa                                            >  1:916562/1‑141 (MQ=255)
                                                                                            ataGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtc                                          <  2:335370/141‑1 (MQ=255)
                                                                                             taGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtca                                         <  1:913020/141‑1 (MQ=255)
                                                                                              aGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtcat                                        >  2:809040/1‑140 (MQ=255)
                                                                                               gCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtcagt                                       <  2:468720/141‑1 (MQ=255)
                                                                                                 tttttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAAtcagtca                                     <  2:591297/141‑1 (MQ=255)
                                                                                                   tttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                   <  2:352606/141‑1 (MQ=255)
                                                                                                   tttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                   <  1:886013/141‑1 (MQ=255)
                                                                                                   tttATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                   <  1:20806/141‑1 (MQ=255)
                                                                                                   tttATATTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCagg                                   >  2:285706/1‑141 (MQ=255)
                                                                                                      gtatTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAGTGGACATTCGAGCGTTAATCAGTCaggagg                                <  2:491687/140‑1 (MQ=255)
                                                                                                      atatTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCaggagg                                >  2:33906/1‑141 (MQ=255)
                                                                                                      atatTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCaggagg                                <  2:70138/141‑1 (MQ=255)
                                                                                                       tatTTGTATAATGAACATATAAGTTTAAGAAGATGAGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAggg                               <  1:190675/141‑1 (MQ=255)
                                                                                                         tttGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGAc                             >  1:684973/1‑141 (MQ=255)
                                                                                                          ttGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGaaa                            <  1:694239/141‑3 (MQ=255)
                                                                                                          ttGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACt                            <  2:217378/141‑1 (MQ=255)
                                                                                                           tGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACtt                           <  1:684333/141‑1 (MQ=255)
                                                                                                                 aTGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACAATCGAGCGTTAATCAGTTAGGAGGGACTTTCactc                     >  2:403135/1‑141 (MQ=255)
                                                                                                                   gAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCcctcct                   <  1:315297/141‑1 (MQ=255)
                                                                                                                     acgtataAGTTGAAGAAGGTGCGAGTGAGGGAAATAAAAAGGACCAAATGTAGCAAAGGGTTAATCTGAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGGGTTAATCAGTCAGGAGGGACTGTcggtcgtac                 >  2:103372/4‑133 (MQ=255)
                                                                                                                      cATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACa                >  2:36375/1‑141 (MQ=255)
                                                                                                                           aaGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTta           >  2:890315/1‑141 (MQ=255)
                                                                                                                            aGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaa          >  1:271063/1‑141 (MQ=255)
                                                                                                                              tttAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaata        >  2:512776/1‑141 (MQ=255)
                                                                                                                                tAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataat      <  2:630735/141‑1 (MQ=255)
                                                                                                                                tAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataat      <  2:985648/141‑1 (MQ=255)
                                                                                                                                tAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataat      <  1:82241/141‑1 (MQ=255)
                                                                                                                                  agaagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTtaataataa    >  2:600604/1‑141 (MQ=255)
                                                                                                                                   gaagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTaataataat   >  2:298622/1‑141 (MQ=255)
                                                                                                                                   gaagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTaataataat   >  1:320746/1‑141 (MQ=255)
                                                                                                                                    aagGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAataataata  >  1:737256/1‑141 (MQ=255)
                                                                                                                                       |                                                                                                                                         
AAAAGATTGGAATGGATCGTCTTGAAATGCTCCCTTCAAAGTTTTCATTTTTTCAATGTCGACTTCGAAGGGGGCATTTTCATTAAATTGTTATAGCTTTTTATATTTGTATAATGAACATATAAGTTTAAGAAGGTGCGAGTGAAGGAAATAAAAAAGCTCAAATGTACCAAATTGTTAATCTTAATAAATCTCTACTTTATAAAGATTGAATGGACATTCGAGCGTTAATCAGTCAGGAGGGACTTTCCCTCCTACAATTTAATAATAATA  >  CP000730/1341798‑1342070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: