Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,411,832 T→C 5.4% H280H (CAT→CAC femA → methicillin resistance factor FemA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,411,8320TC 5.4% 264.6 / 3.8 91H280H (CAT→CACfemAmethicillin resistance factor FemA
Reads supporting (aligned to +/- strand):  ref base T (46/40);  new base C (3/2);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GACCGTGTGTTAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGAAGTTGTTT  >  CP000730/1411692‑1411971
                                                                                                                                            |                                                                                                                                           
gACCGTGTGTTAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACAt                                                                                                                                             >  2:931539/1‑141 (MQ=255)
 aCCGTGTGTTAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATa                                                                                                                                            >  1:759300/1‑141 (MQ=255)
   cGTGTGTTAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAAc                                                                                                                                          >  2:532457/1‑141 (MQ=255)
   cGTGTGTTAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAAc                                                                                                                                          >  2:360074/1‑141 (MQ=255)
   cGTGTGTTAGTACCTATAGAAAAAAACAAATTTGATGAATATATTAAAGAACTAAACGAAGAGCCTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGAAAAAAAAAAAAAAAAGCACATAAc                                                                                                                                          <  2:669304/141‑1 (MQ=255)
     tgtgtTAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACaa                                                                                                                                        >  2:880226/1‑141 (MQ=255)
      gtgtTAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTTATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACaaa                                                                                                                                       >  2:87710/1‑140 (MQ=255)
       tgtTAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGc                                                                                                                                      <  2:671378/141‑1 (MQ=255)
        gtTAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCg                                                                                                                                     >  2:874838/1‑141 (MQ=255)
         ttAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCga                                                                                                                                    <  1:740586/141‑1 (MQ=255)
         ttAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCga                                                                                                                                    <  2:84865/141‑1 (MQ=255)
          tAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAAAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCgag                                                                                                                                   >  1:548634/1‑141 (MQ=255)
            gTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGAAAAAAAAAAAAAAAAGCACATAACAAGCGAGAt                                                                                                                                 <  2:892759/141‑1 (MQ=255)
               ccTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAAc                                                                                                                              >  2:956590/1‑141 (MQ=255)
                cTTTAGCGTATATAAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACt                                                                                                                             <  1:97173/141‑1 (MQ=255)
                  ttAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTa                                                                                                                           >  1:734725/1‑141 (MQ=255)
                  ttAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTa                                                                                                                           <  1:288724/141‑1 (MQ=255)
                     gCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTacaa                                                                                                                        >  2:366182/1‑141 (MQ=255)
                      cGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTacaac                                                                                                                       >  2:696293/1‑141 (MQ=255)
                      cGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTacaac                                                                                                                       >  1:660651/1‑141 (MQ=255)
                      cGTATATCAAATTTGATGAATATAATAAAGAAATAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAAAGAAAAAAAAAAAAAAAAGCACATAACAAGCGAGATAACTTacaac                                                                                                                       <  2:1034911/141‑1 (MQ=255)
                       gTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTacaaca                                                                                                                      <  1:669495/141‑1 (MQ=255)
                       atataTCAACTTTAATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTacaaca                                                                                                                      <  2:545424/140‑1 (MQ=255)
                        tataTCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTacaacaa                                                                                                                     <  2:865453/141‑1 (MQ=255)
                          tatCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGATAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTacaacaaca                                                                                                                   >  2:1055863/1‑141 (MQ=255)
                             cAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACt                                                                                                                >  2:519194/1‑141 (MQ=255)
                             cAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACt                                                                                                                >  1:170461/1‑141 (MQ=255)
                              aaCTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACtt                                                                                                               >  2:493122/1‑141 (MQ=255)
                              aaCTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACtt                                                                                                               <  1:962002/141‑1 (MQ=255)
                              aaCTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTGAAAGGATATTGAAAAACGTCCTGAAAAGAAAAAAGCACATAACAAGCGAGATGACTTACAACAACAACtt                                                                                                               >  2:787101/1‑141 (MQ=255)
                                  ttGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATg                                                                                                           >  1:117377/1‑141 (MQ=255)
                                  ttGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATg                                                                                                           >  1:117491/1‑141 (MQ=255)
                                   tgatgaATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGc                                                                                                          >  2:256207/1‑141 (MQ=255)
                                    gatgaATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCa                                                                                                         >  2:187153/1‑141 (MQ=255)
                                    gatgaATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACACAACAAGCGAGATAACTTACAACAACAACTTGATGct                                                                                                         >  1:769889/1‑140 (MQ=255)
                                    gatgaATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCAAACAACAAGCGAGATAACTTACAACAACAAATTGATgat                                                                                                         >  2:92503/1‑139 (MQ=255)
                                     atgaATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCaa                                                                                                        >  2:950865/1‑141 (MQ=255)
                                      tgaATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCaaa                                                                                                       >  1:932515/1‑141 (MQ=255)
                                      tgaATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCaaa                                                                                                       <  1:471076/141‑1 (MQ=255)
                                      tgaATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACACAACAAGCGAGATAACTTACAACAACAACTTGATGctaa                                                                                                       >  2:848316/1‑138 (MQ=255)
                                        aagagatTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAAATTGATGCAAATg                                                                                                     >  2:482752/6‑141 (MQ=255)
                                             atTAAAGAAGTAAACTAATGGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATGGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCaa                                                                                                <  2:586170/141‑1 (MQ=255)
                                             atTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCaa                                                                                                <  1:700015/141‑1 (MQ=255)
                                              ttAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGATAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCaaa                                                                                               >  2:927871/1‑141 (MQ=255)
                                               tAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCaaaa                                                                                              >  1:368818/1‑141 (MQ=255)
                                                aaaGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAg                                                                                             <  2:124023/141‑1 (MQ=255)
                                                 aaGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGa                                                                                            <  1:23570/141‑1 (MQ=255)
                                                 aaGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACACAACAAGCGAGATAACTTACAACAACAACTTGATGCTAATGAGCAAAAGa                                                                                            <  1:517713/141‑1 (MQ=255)
                                                     aCTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTga                                                                                        >  1:41684/1‑141 (MQ=255)
                                                     aCTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTga                                                                                        <  1:170089/141‑1 (MQ=255)
                                                          ttttattttttGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATACAAATGAGCAAAAGATTGaagaag                                                                                   >  2:768755/11‑141 (MQ=255)
                                                          atgAAGATCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCATATAACGGTCGAGAGAACTTACAACAACATCTTGATGCAAATGAGCAAAAGATTTaagtag                                                                                   <  2:491373/139‑1 (MQ=255)
                                                          aagAAGAGCGTGATATTTTAAATAAAAATTTAAATAAAGCGTTAAAGTATATTGAAAAACGTACTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGaagaag                                                                                   <  2:472622/139‑1 (MQ=255)
                                                          aCGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGaagaag                                                                                   >  1:139892/1‑141 (MQ=255)
                                                            gAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGt                                                                                 >  1:598956/1‑141 (MQ=255)
                                                              agagCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTaa                                                                               <  1:999745/141‑1 (MQ=255)
                                                               gagCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTaaa                                                                              <  1:226097/141‑1 (MQ=255)
                                                                 gCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACg                                                                            >  1:436859/1‑141 (MQ=255)
                                                                  cGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGt                                                                           <  2:734725/141‑1 (MQ=255)
                                                                         ttttAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACaag                                                                    <  1:333870/141‑1 (MQ=255)
                                                                              aataaaGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAAAAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAgaagaa                                                               >  1:76606/1‑141 (MQ=255)
                                                                                   aGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATgg                                                          <  1:198350/141‑1 (MQ=255)
                                                                                    gATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGt                                                         >  1:448464/1‑141 (MQ=255)
                                                                                       ttAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAAt                                                      <  2:7509/141‑1 (MQ=255)
                                                                                          aataaaGCGTTAAAGGATCTTGAAAAACGCCCTGAAAATAAAAAAGCACATAACAAGCGAGCTAACTTACAACAACAACTTGATGCAAATGAGCAACAGATTGAAGAAGGTAAACGTCTACAAGAAGACCATGGTAATGac                                                   >  2:824973/1‑140 (MQ=255)
                                                                                          aataaaGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGaa                                                   >  1:930605/1‑141 (MQ=255)
                                                                                           ataaaGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAAt                                                  >  1:335831/1‑141 (MQ=255)
                                                                                                gCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCt                                             <  2:335831/141‑1 (MQ=255)
                                                                                                gCGATAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCt                                             >  2:403520/1‑141 (MQ=255)
                                                                                                   ttAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACACAACAAGCGAGATAACTTACAACAACAACTTGATGCTAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTAtc                                          <  1:848316/141‑1 (MQ=255)
                                                                                                        tgATATTTAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATCAATTACCTATATccgc                                     <  2:1024333/140‑1 (MQ=255)
                                                                                                          atatTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTctgctg                                   <  2:929427/141‑1 (MQ=255)
                                                                                                           tatTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTgg                                  <  2:660651/141‑1 (MQ=255)
                                                                                                              tGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGttt                               <  2:709697/141‑1 (MQ=255)
                                                                                                              tGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGAGTCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGttt                               <  1:293619/141‑1 (MQ=255)
                                                                                                               gAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTANCTATCTCTGCTGGTttc                              >  1:733066/1‑141 (MQ=255)
                                                                                                                aaaaaCGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTttct                             >  1:326153/1‑141 (MQ=255)
                                                                                                                      gTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTa                       >  2:473817/1‑141 (MQ=255)
                                                                                                                           gAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAAt                  <  2:658650/141‑1 (MQ=255)
                                                                                                                           gAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAAt                  <  1:338595/141‑1 (MQ=255)
                                                                                                                           gAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAAt                  <  2:903886/141‑1 (MQ=255)
                                                                                                                            aaaaTAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATc                 <  1:564516/141‑1 (MQ=255)
                                                                                                                             aaaTAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATcc                >  1:776764/1‑141 (MQ=255)
                                                                                                                                 aaaaaaGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCAttt            >  1:75056/1‑141 (MQ=255)
                                                                                                                                  aaaaaGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTg           >  2:828183/1‑141 (MQ=255)
                                                                                                                                    aaaGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGaa         <  1:961047/141‑1 (MQ=255)
                                                                                                                                    aaaGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGaa         >  2:432964/1‑141 (MQ=255)
                                                                                                                                    aaaGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATCACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGaa         >  1:69057/1‑141 (MQ=255)
                                                                                                                                    aaaGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATCACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGaa         >  1:69104/1‑141 (MQ=255)
                                                                                                                                     aaGCACATAACAAGCGAGATAACTTACAACAACAACGTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGAAg        <  2:534596/141‑1 (MQ=255)
                                                                                                                                      aGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGAAgt       <  1:670193/141‑1 (MQ=255)
                                                                                                                                           aTAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGAAGTTGttt  <  2:969783/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GACCGTGTGTTAGTACCTTTAGCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGAAGTTGTTT  >  CP000730/1411692‑1411971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: