Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,436,095 G→C 6.2% G197A (GGT→GCT)  hipO → M20D subfamily hippurate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,436,0950GC 6.2% 268.6 / 9.5 81G197A (GGT→GCT) hipOM20D subfamily hippurate hydrolase
Reads supporting (aligned to +/- strand):  ref base G (35/41);  new base C (2/3);  total (37/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.22e-01

AGCGGTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGT  >  CP000730/1435955‑1436234
                                                                                                                                            |                                                                                                                                           
aGCGGTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAgg                                                                                                                                             >  1:442112/1‑141 (MQ=255)
 gCGGTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGt                                                                                                                                            <  2:107704/141‑1 (MQ=255)
 gCGGTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGt                                                                                                                                            <  1:548257/141‑1 (MQ=255)
  cGGTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTc                                                                                                                                           <  2:631598/141‑1 (MQ=255)
  cGGTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTc                                                                                                                                           >  1:234703/1‑141 (MQ=255)
  cGGTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTc                                                                                                                                           >  2:776066/1‑141 (MQ=255)
   ggTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCa                                                                                                                                          >  1:997806/1‑141 (MQ=255)
   ggTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCa                                                                                                                                          >  2:835042/1‑141 (MQ=255)
    gTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCAt                                                                                                                                         <  2:865985/141‑1 (MQ=255)
      aTTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTc                                                                                                                                       <  1:26017/141‑1 (MQ=255)
        ttGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCtt                                                                                                                                     >  2:802186/1‑141 (MQ=255)
           gTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTtgtg                                                                                                                                  <  1:463697/141‑1 (MQ=255)
            tATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTgtgg                                                                                                                                 <  1:791678/141‑1 (MQ=255)
             aTTCATGTTAACCCATTTGCACAGGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTgtggt                                                                                                                                <  1:826150/141‑1 (MQ=255)
               tCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGa                                                                                                                              <  2:110626/141‑1 (MQ=255)
                cATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGaa                                                                                                                             >  2:897231/1‑141 (MQ=255)
                      aaCCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTa                                                                                                                       >  2:10729/1‑141 (MQ=255)
                        cccATTTNCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACCAGGTCATTCTTGTGGTGAAGCATTaca                                                                                                                     <  2:816616/141‑1 (MQ=255)
                        cccATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTaca                                                                                                                     <  2:242552/141‑1 (MQ=255)
                        cccATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTaca                                                                                                                     <  1:543284/141‑1 (MQ=255)
                        cccATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTaca                                                                                                                     <  2:788334/141‑1 (MQ=255)
                            tttGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACAt                                                                                                                 >  2:1041322/1‑141 (MQ=255)
                            tttGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGCTCATTCTTGTGGTGAAGCATTACAACAt                                                                                                                 >  1:1050070/1‑141 (MQ=255)
                            tttGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGCTCATTCTTGTGGTGAAGCATTACAACAt                                                                                                                 >  1:1049995/1‑141 (MQ=255)
                             ttGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATg                                                                                                                <  2:187352/141‑1 (MQ=255)
                               gCTGATGAAGGCATTACAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTa                                                                                                              >  2:901533/1‑141 (MQ=255)
                                cTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTAt                                                                                                             <  2:381868/141‑1 (MQ=255)
                                 tgatgaAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACATGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTAtt                                                                                                            >  2:682995/1‑141 (MQ=255)
                                  gatgaAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTa                                                                                                           >  2:861881/1‑141 (MQ=255)
                                  gatgaAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTa                                                                                                           >  1:199834/1‑141 (MQ=255)
                                    tgaAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAAc                                                                                                         <  2:342213/141‑1 (MQ=255)
                                           aTTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAata                                                                                                  >  2:659385/1‑141 (MQ=255)
                                            ttGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAatat                                                                                                 >  1:760327/1‑141 (MQ=255)
                                              gCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATca                                                                                               >  2:835973/1‑141 (MQ=255)
                                                aGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATcaca                                                                                             >  2:353179/1‑141 (MQ=255)
                                                aGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATcaca                                                                                             >  1:453481/1‑141 (MQ=255)
                                                 gTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACaa                                                                                            >  2:6384/1‑141 (MQ=255)
                                                  tGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTAAAACATGTATTAACTCAAATATCACaaa                                                                                           >  2:377787/1‑141 (MQ=255)
                                                    aTAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGCTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAAtt                                                                                         <  1:28799/141‑1 (MQ=255)
                                                      aaGAGATGAAGAAATTACTGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTca                                                                                       <  1:875131/141‑1 (MQ=255)
                                                      aaGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTca                                                                                       <  2:611943/141‑1 (MQ=255)
                                                       agagATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTcaa                                                                                      <  1:362851/141‑1 (MQ=255)
                                                       agagATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTcaa                                                                                      >  2:128220/1‑141 (MQ=255)
                                                       agagATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTcaa                                                                                      <  2:298661/141‑1 (MQ=255)
                                                           aTGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAAt                                                                                  >  1:343369/1‑141 (MQ=255)
                                                           aTGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAAt                                                                                  >  1:823899/1‑141 (MQ=255)
                                                             gaagaaATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAAttt                                                                                <  1:197152/141‑1 (MQ=255)
                                                             gaagaaATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTAGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAAttt                                                                                >  1:754760/1‑141 (MQ=255)
                                                                       cGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACg                                                                      >  2:1025779/1‑141 (MQ=255)
                                                                       cGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACg                                                                      <  2:1001301/141‑1 (MQ=255)
                                                                                 aaCAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACg                                                            >  2:668398/1‑141 (MQ=255)
                                                                                  aCAGAGTATCGCTTTTTTTTTTTTTGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGa                                                           >  2:779010/1‑141 (MQ=255)
                                                                                  aCAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGa                                                           <  1:901533/141‑1 (MQ=255)
                                                                                   cAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGaa                                                          >  1:721305/1‑141 (MQ=255)
                                                                                   cAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGaa                                                          <  1:110972/141‑1 (MQ=255)
                                                                                       gTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAatat                                                      <  1:1026504/141‑1 (MQ=255)
                                                                                         aTCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGCTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTg                                                    <  1:133656/141‑1 (MQ=255)
                                                                                          tCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGt                                                   <  2:670712/141‑1 (MQ=255)
                                                                                          tCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGt                                                   <  1:1036524/141‑1 (MQ=255)
                                                                                          tCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGCTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGt                                                   <  2:819335/141‑1 (MQ=255)
                                                                                           cGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGtt                                                  >  1:725295/1‑141 (MQ=255)
                                                                                                tttCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCatat                                             <  1:707920/141‑1 (MQ=255)
                                                                                                    ttAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGt                                         <  1:369028/141‑1 (MQ=255)
                                                                                                        cAGGCCAGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTGTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCAtt                                     <  2:1036786/141‑1 (MQ=255)
                                                                                                         aGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCCTTCAAGTGGTGAAGCATTACAACATGTATTAACCCAAATATCACAAATTCAACAATTTCACCTTAACGGCTTGAAACGAAATATTGTTCATATTGGTCAttt                                    >  2:775335/1‑141 (MQ=255)
                                                                                                         aGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCAttt                                    <  2:199834/141‑1 (MQ=255)
                                                                                                         aGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCAttt                                    <  1:35996/141‑1 (MQ=255)
                                                                                                          ggCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCAtttt                                   <  1:829244/141‑1 (MQ=255)
                                                                                                           gCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTa                                  <  2:500643/141‑1 (MQ=255)
                                                                                                           gCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTa                                  <  1:5954/141‑1 (MQ=255)
                                                                                                            ccTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTaa                                 >  2:291905/1‑141 (MQ=255)
                                                                                                              tGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAg                               >  2:278667/1‑141 (MQ=255)
                                                                                                                 cAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTg                            <  1:744554/141‑1 (MQ=255)
                                                                                                                  aaGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTgg                           >  1:381434/1‑141 (MQ=255)
                                                                                                                  aaGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTgg                           <  2:343369/141‑1 (MQ=255)
                                                                                                                        tGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGc                     >  2:650186/1‑141 (MQ=255)
                                                                                                                            gCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGATGGTGAAGCGAtt                 >  1:741599/1‑141 (MQ=255)
                                                                                                                             cTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTa                <  1:172795/141‑1 (MQ=255)
                                                                                                                                aTAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAaca             <  1:141767/141‑1 (MQ=255)
                                                                                                                                 tAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAacac            <  2:943980/141‑1 (MQ=255)
                                                                                                                                      aaCAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTAc       <  2:116567/141‑1 (MQ=255)
                                                                                                                                       aCAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTAcc      >  2:1047571/1‑141 (MQ=255)
                                                                                                                                           ggTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGt  >  2:390748/1‑141 (MQ=255)
                                                                                                                                           ggTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGt  <  2:581062/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
AGCGGTATTTGGTATTCATGTTAACCCATTTGCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTATCGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGTGGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAACGGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACTGTACCAAGT  >  CP000730/1435955‑1436234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑