Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,451,633 C→T 8.2% A763A (GCG→GCA sucA ← oxoglutarate dehydrogenase (succinyl‑transferring) E1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,451,6330CT 8.2% 241.4 / 5.2 83A763A (GCG→GCAsucAoxoglutarate dehydrogenase (succinyl‑transferring) E1
Reads supporting (aligned to +/- strand):  ref base C (32/43);  new base T (4/3);  total (36/47)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTGATATGATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCATGAGG  >  CP000730/1451493‑1451773
                                                                                                                                             |                                                                                                                                            
gCTTGATATGATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGcc                                                                                                                                              <  1:871333/141‑1 (MQ=255)
  ttGATATGATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGc                                                                                                                                            <  1:212750/141‑1 (MQ=255)
   tGATATGATTCTGTCAAAATTGGCGCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCt                                                                                                                                           <  1:46984/141‑1 (MQ=255)
      tatGATTCTGTCAAAATTGGCTCAAATCCGCCAGAAGTACATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGCGGCATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACGAGCGGCTtgt                                                                                                                                        <  2:130719/141‑1 (MQ=255)
      tatGATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTtgt                                                                                                                                        >  1:1016572/1‑141 (MQ=255)
         gATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCa                                                                                                                                     >  2:302790/1‑141 (MQ=255)
         gATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCa                                                                                                                                     <  2:852319/141‑1 (MQ=255)
             cTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCa                                                                                                                                 >  2:201331/1‑141 (MQ=255)
              tGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGTTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAAACTAAACTAGCCGCTTGTGCACGCaa                                                                                                                                <  2:164338/141‑1 (MQ=255)
               gTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAAt                                                                                                                               >  1:175152/1‑141 (MQ=255)
               gTCAAAATTGGCTCAAATCCACCAGAAGTAAACTCATCAATTGGTTTGGCAACTGTTTTATTTCTCAATAAGCTTTTTTTTTTTTTTTCAACCAATGGTAGCATTTGTTCATTTTCTAAACTAGCTGCTTGTTAACGCAAt                                                                                                                               >  2:604220/1‑141 (MQ=255)
                tCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATa                                                                                                                              >  2:683492/1‑141 (MQ=255)
                tCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATa                                                                                                                              <  2:428222/141‑1 (MQ=255)
                tCAAAATTGGCTCAAATCCACCAGAAGTAAACTCATCAATTGGTTTGGCAACTGTTTTATTTCTCAATAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATa                                                                                                                              >  1:992103/1‑141 (MQ=255)
                      tttgCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCAGTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGa                                                                                                                        <  2:255228/138‑1 (MQ=255)
                      ttGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGa                                                                                                                        <  1:265476/141‑1 (MQ=255)
                      ttGGCTCAAATCCACCAGAAGTAAACTCATCAATTGGTTTGGCAACTGTTTTATTTCTCAATAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATAAGTGGa                                                                                                                        <  1:944587/141‑1 (MQ=255)
                       tGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGaa                                                                                                                       <  1:421061/141‑1 (MQ=255)
                       tGGCTCAAATCCACCAGAAGTAAACTCATCAATTGGTTTGGCAACTGTTTTATTTCTCAATAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATAAGTGGaa                                                                                                                       >  1:17662/1‑141 (MQ=255)
                         gCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAaat                                                                                                                     >  1:78194/1‑141 (MQ=255)
                           tCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAaataa                                                                                                                   <  1:50707/141‑1 (MQ=255)
                            cAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAaataat                                                                                                                  <  2:231791/141‑1 (MQ=255)
                            cAAATCCACCAGAAGTAAATTCATCAATTGGTTNTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAaataat                                                                                                                  >  1:906574/1‑141 (MQ=255)
                            cAAATCCAACAGAAGTACACTAACCAATCGGTTTTGCAAGCGTTTTATTTCTCAGTAAGCTTTTTTGGTGACATCACAGCGAATGGTCGCATTTGTTTAGAATCTAACCTAGCCGCGTGTGCACGCAACAAGAGGAaataa                                                                                                                   >  2:1083049/1‑141 (MQ=255)
                              aaTCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTa                                                                                                                <  1:790344/141‑1 (MQ=255)
                                         aGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTTCACGCAATAAGTGGAGATAATTACTTGAACTAGa                                                                                                     >  1:294715/1‑141 (MQ=255)
                                           tAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATa                                                                                                   <  1:573540/141‑1 (MQ=255)
                                           tAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATa                                                                                                   <  1:643017/141‑1 (MQ=255)
                                             aaTTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAg                                                                                                 <  2:720596/141‑1 (MQ=255)
                                              aTTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGt                                                                                                >  1:1070122/1‑141 (MQ=255)
                                               ctcatcaATTGGTTTGGCAACTGTTTTATTTCTCAATAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGtt                                                                                               >  1:190634/2‑141 (MQ=255)
                                                tcatcaATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTg                                                                                              >  1:168557/1‑141 (MQ=255)
                                                         ggTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACtgtg                                                                                     >  2:386958/1‑141 (MQ=255)
                                                         ggTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACtgtg                                                                                     >  1:447805/1‑141 (MQ=255)
                                                         ggTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACtgtg                                                                                     <  1:912216/141‑1 (MQ=255)
                                                         ggTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACtgtg                                                                                     <  2:396658/141‑1 (MQ=255)
                                                          gTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGc                                                                                    <  1:167113/141‑1 (MQ=255)
                                                           ttttGCAACTGTTTTATTTCTCAGTAAGCTTTTTTTTTTCATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTATGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCa                                                                                   >  2:863466/1‑141 (MQ=255)
                                                           ttttGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCa                                                                                   <  2:481565/141‑1 (MQ=255)
                                                             ttGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAat                                                                                 >  1:747472/1‑141 (MQ=255)
                                                             ttGCAACTGTTCTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAat                                                                                 <  1:766561/141‑1 (MQ=255)
                                                              tGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGCCATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAatt                                                                                <  2:999762/141‑1 (MQ=255)
                                                                    tGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTc                                                                          <  1:201331/141‑1 (MQ=255)
                                                                      ttttATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTcag                                                                        <  1:57522/141‑1 (MQ=255)
                                                                          aTTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCagcagc                                                                    >  1:689680/1‑141 (MQ=255)
                                                                          aTTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCagcagc                                                                    <  1:951384/141‑1 (MQ=255)
                                                                               tcAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAAtt                                                               >  1:472660/1‑141 (MQ=255)
                                                                               tcAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAAtt                                                               >  1:550038/1‑141 (MQ=255)
                                                                                cAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTg                                                              <  1:190706/141‑1 (MQ=255)
                                                                                       cTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAAt                                                       >  1:228394/1‑141 (MQ=255)
                                                                                         tttttGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATc                                                      >  1:641227/1‑141 (MQ=255)
                                                                                            ttGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGct                                                   <  1:857335/141‑1 (MQ=255)
                                                                                              ggTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGATAAATGTAAAAATCGctct                                                 <  2:396710/141‑1 (MQ=255)
                                                                                                  aCATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATc                                             <  1:712669/141‑1 (MQ=255)
                                                                                                  aCATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATc                                             >  1:515214/1‑141 (MQ=255)
                                                                                                     tAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTg                                          <  2:78194/141‑1 (MQ=255)
                                                                                                     tAACAACAAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGAAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGAAAAAAAAAAAAAAAATCGCTCTAATCTTg                                          <  2:366210/141‑1 (MQ=255)
                                                                                                       acaacCAATGGTCGCATTTGTTCAGAATCTCAACTAGCCGCTCGCGCACGCCATACGTGGAAATACTTACTAGCACCAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTATTTGTAACACTTGCTCTAAtaataga                                        >  2:589145/1‑135 (MQ=255)
                                                                                                       acaacCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCt                                        >  1:794936/1‑141 (MQ=255)
                                                                                                        caacCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGatctggtgcctatg                                       >  2:134608/1‑131 (MQ=255)
                                                                                                        caacCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCtg                                       >  2:754309/1‑141 (MQ=255)
                                                                                                        caacCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCtg                                       <  1:929174/141‑1 (MQ=255)
                                                                                                        caacCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCGCTAATCTTGCtg                                       >  1:266888/1‑141 (MQ=255)
                                                                                                         aacCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCtga                                      <  2:447805/141‑1 (MQ=255)
                                                                                                             aaTGGTCGCATTTGTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGaa                                  <  2:992103/141‑1 (MQ=255)
                                                                                                             aaTGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGaa                                  <  1:302790/141‑1 (MQ=255)
                                                                                                                ggTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGt                               >  2:412764/1‑141 (MQ=255)
                                                                                                                 gTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGtt                              <  2:55293/141‑1 (MQ=255)
                                                                                                                  tCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTc                             >  1:981320/1‑141 (MQ=255)
                                                                                                                   cGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCa                            >  1:993363/1‑141 (MQ=255)
                                                                                                                   cGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCa                            >  1:74195/1‑141 (MQ=255)
                                                                                                                     cATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAgg                          <  2:994569/141‑1 (MQ=255)
                                                                                                                     cATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAgg                          <  2:461330/141‑1 (MQ=255)
                                                                                                                      aTTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGc                         <  1:444946/141‑1 (MQ=255)
                                                                                                                          gTTCAGAATCTAAACTAGCTGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGTCCtt                     <  2:1074060/141‑1 (MQ=255)
                                                                                                                              aGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGAcc                 <  1:155705/141‑1 (MQ=255)
                                                                                                                              aGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGAcc                 <  2:420469/141‑1 (MQ=255)
                                                                                                                                 aTCTAAACTAGCCGCTTGTGCACGCAATAAGTGGTAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTc              <  1:1007185/141‑1 (MQ=255)
                                                                                                                                    tAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCatg           <  1:805227/141‑2 (MQ=255)
                                                                                                                                    tAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCata           >  2:109870/1‑141 (MQ=255)
                                                                                                                                    tAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCata           <  1:808220/141‑1 (MQ=255)
                                                                                                                                        cTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCa       >  1:362467/1‑141 (MQ=255)
                                                                                                                                         tAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCAt      <  2:492443/141‑1 (MQ=255)
                                                                                                                                           gCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCATGa    <  2:544314/141‑1 (MQ=255)
                                                                                                                                             cGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCATGAgg  >  2:613849/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                            
GCTTGATATGATTCTGTCAAAATTGGCTCAAATCCACCAGAAGTAAATTCATCAATTGGTTTTGCAACTGTTTTATTTCTCAGTAAGCTTTTTGGTGACATAACAACCAATGGTCGCATTTGTTCAGAATCTAAACTAGCCGCTTGTGCACGCAATAAGTGGAAATAATTACTTGAACTAGATAAGTTGACAACTGTGCAATTATTTTCAGCAGCTAATTGTAAAAATCGCTCTAATCTTGCTGATGAATGTTCAGGCCCTTGACCCTCATATGCATGAGG  >  CP000730/1451493‑1451773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: