Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,454,646	T→C	5.3%	A305A (GCA→GCG)	arlS ←	sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,454,646	0	T	C	5.3%	227.5 / 2.9	76	A305A (GCA→GCG)	arlS	sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (41/31);  new base C (2/2);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

AATAATTGTTCGAATTGATGAGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGTCTTTTTTTCCCCATCGCTG  >  CP000730/1454506‑1454786                                                                                                                                             |                                                                                                                                             aataatTGTTCGAATTGATGAGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCtgt                                                                                                                                              <  2:557967/141‑1 (MQ=255) aataatTGTTCGAATTGATGAGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCtgt                                                                                                                                              <  2:1056690/141‑1 (MQ=255)  ataatTGTTCGAATTGATGAGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCtgtg                                                                                                                                             >  2:671473/1‑141 (MQ=255)    aatTGTTCGAATTGATGAGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCt                                                                                                                                           <  2:1009571/141‑1 (MQ=255)     atTGTTCGAATTGATGAGGTTTCATTTTAATTTCTAGATTTTTATATTTCTGATTCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATTAGTGTATTCGCGAGCGAATTTCATCATTTATATGAACGGTTTGTGCtt                                                                                                                                          >  2:196944/1‑141 (MQ=255)      ttGTTCGAATTGATGAGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTc                                                                                                                                         <  2:114228/141‑1 (MQ=255)        gTTCGAATTGATGAGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCag                                                                                                                                       >  1:177092/1‑141 (MQ=255)               ttGATGAGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAAt                                                                                                                                <  1:668680/141‑1 (MQ=255)               ttGATGAGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAAt                                                                                                                                <  1:563113/141‑1 (MQ=255)                tgatgaGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATg                                                                                                                               <  2:305277/141‑1 (MQ=255)                  atgaGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGCGCTTCAGAAGAAATGTc                                                                                                                             >  1:95237/1‑141 (MQ=255)                       gTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTa                                                                                                                        <  2:633541/141‑1 (MQ=255)                           cATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACAtc                                                                                                                    <  2:408010/141‑1 (MQ=255)                           cATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACAtc                                                                                                                    <  2:641900/141‑1 (MQ=255)                           cATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGCGCTTCAGAAGAAATGTCATTTACAtc                                                                                                                    >  1:99847/1‑141 (MQ=255)                            aTTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGCGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACAtct                                                                                                                   <  2:577863/141‑1 (MQ=255)                             ttttAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCTTTAACAtctc                                                                                                                  >  2:1028423/1‑141 (MQ=255)                                tAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCtt                                                                                                               <  1:261517/141‑1 (MQ=255)                                tAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCtt                                                                                                               >  2:741982/1‑141 (MQ=255)                                tAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCtt                                                                                                               >  1:638001/1‑141 (MQ=255)                                      cTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCa                                                                                                         >  1:478594/1‑141 (MQ=255)                                       tAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCaa                                                                                                        <  1:739539/141‑1 (MQ=255)                                             tttAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAg                                                                                                  <  2:123097/141‑1 (MQ=255)                                             tttAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAg                                                                                                  >  2:140014/1‑141 (MQ=255)                                             tttAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAg                                                                                                  <  2:997000/141‑1 (MQ=255)                                               tAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTa                                                                                                <  1:80887/141‑1 (MQ=255)                                                aGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTaa                                                                                               >  1:38692/1‑141 (MQ=255)                                                aGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTaa                                                                                               >  1:114946/1‑141 (MQ=255)                                                 gATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAAt                                                                                              >  2:74545/1‑141 (MQ=255)                                                   tGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAAttc                                                                                            >  2:492625/1‑141 (MQ=255)                                                     ttagttCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTATATCTCCTTTAGTCAATTCAAGTAAttctt                                                                                          >  2:228648/6‑141 (MQ=255)                                                     tCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAAttctt                                                                                          <  1:17972/141‑1 (MQ=255)                                                      cAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAAttcttc                                                                                         <  2:773896/141‑1 (MQ=255)                                                         aTCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGAc                                                                                      >  1:135970/1‑141 (MQ=255)                                                         aTCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGAc                                                                                      >  2:594746/1‑141 (MQ=255)                                                             gTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGCGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAAt                                                                                  <  2:583780/141‑1 (MQ=255)                                                              tATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAAtt                                                                                 >  1:31260/1‑141 (MQ=255)                                                                tCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCCTTAGTCAATTCAAGTAATTCTTCGACTAAtttt                                                                               >  2:697106/1‑141 (MQ=255)                                                                  aaaTTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTAt                                                                             <  1:310406/141‑1 (MQ=255)                                                                  aaaTTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTAt                                                                             <  2:982448/141‑1 (MQ=255)                                                                     ttGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGAt                                                                          <  1:814963/141‑1 (MQ=255)                                                                      tGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATa                                                                         >  1:588318/1‑141 (MQ=255)                                                                        aTAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCCCGGTCTATGCATAAGAAGAACTGTAATATCAAATTCCCTACGTCAATGTTAGTAATTCTTCGACTAATTTTATGATACg                                                                       >  1:973164/1‑141 (MQ=255)                                                                           aTCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGAtt                                                                    >  1:763429/1‑141 (MQ=255)                                                                                  tGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATttct                                                             <  2:1055052/141‑1 (MQ=255)                                                                                    cAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATttcttc                                                           >  1:13093/1‑141 (MQ=255)                                                                                      aTTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCaa                                                         <  1:3427/141‑1 (MQ=255)                                                                                        tGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAGATGTCATTTACAACTCCTTTAGGAACTTAAACTAATTCTTCGACTAATTTTATGATCCAATTGATTTCTTCAATa                                                       >  1:236711/1‑141 (MQ=255)                                                                                             tAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAat                                                  >  1:987701/1‑141 (MQ=255)                                                                                              aaTGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAata                                                 >  2:24811/1‑141 (MQ=255)                                                                                              aaTGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAata                                                 >  2:90246/1‑141 (MQ=255)                                                                                               aTGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAatat                                                >  1:356791/1‑141 (MQ=255)                                                                                                  aGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTa                                             <  1:411531/141‑1 (MQ=255)                                                                                                   gtgtATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTaa                                            <  1:879649/141‑1 (MQ=255)                                                                                                   gtgtATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTaa                                            >  1:989206/1‑141 (MQ=255)                                                                                                   gtgtATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTaa                                            >  1:60731/1‑141 (MQ=255)                                                                                                   gtgtATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTaa                                            >  2:817997/1‑141 (MQ=255)                                                                                                    tgtATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAAc                                           <  1:741982/141‑1 (MQ=255)                                                                                                      tATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGa                                         <  1:962303/141‑1 (MQ=255)                                                                                                          cgcgAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCGATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGAttct                                     >  1:398929/1‑141 (MQ=255)                                                                                                          cgcgAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGAttct                                     >  1:238597/1‑141 (MQ=255)                                                                                                          cgcgAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGAttct                                     >  1:79653/1‑141 (MQ=255)                                                                                                             gagcgaATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATtcttct                                  <  1:182938/141‑1 (MQ=255)                                                                                                              agcgaATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTa                                 <  1:249885/141‑1 (MQ=255)                                                                                                                    tttCATCATGAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATActg                           >  1:952921/1‑141 (MQ=255)                                                                                                                     ttCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATActgc                          >  2:722470/1‑141 (MQ=255)                                                                                                                       catcatTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATActgctg                        >  1:518443/1‑141 (MQ=255)                                                                                                                        atcatTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGCCTCCTCTTATGATCGGCTTCATTTCTTCAATAGAAATATTTAACGCTTCTTCTAATACTGCTgg                       >  1:267523/1‑141 (MQ=255)                                                                                                                         tcatTAATATGCACGGTCTGCGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTggg                      <  2:180778/141‑1 (MQ=255)                                                                                                                          catTAATATGCACTGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAATCAGGCCTAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGt                     <  1:782349/141‑1 (MQ=255)                                                                                                                          catTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGt                     >  2:605707/1‑141 (MQ=255)                                                                                                                           atTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTACTGGGTc                    <  1:1044606/141‑1 (MQ=255)                                                                                                                           atTAATATGCACCGTCTGTGCTTTAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTACTGGGTc                    <  2:688928/141‑1 (MQ=255)                                                                                                                                  tGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGTCttttttt             >  2:730016/1‑141 (MQ=255)                                                                                                                                     aCGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGTCTTTTTTTccc          >  1:472244/1‑141 (MQ=255)                                                                                                                                     aCGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGTCTTTTTTTccc          >  2:605411/1‑141 (MQ=255)                                                                                                                                     aCGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGTCTTTTTTTccc          >  1:588826/1‑141 (MQ=255)                                                                                                                                      cGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGTCTTTTTTTcccc         <  1:471601/141‑1 (MQ=255)                                                                                                                                        gTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGTCTTTTTTTCCCCAt       >  1:408181/1‑141 (MQ=255)                                                                                                                                             tgCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGTCTTTTTTTCCCCATCGCTg  >  2:772036/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                             AATAATTGTTCGAATTGATGAGGTTTCATTTTAATTTCTAGATTTTTAGATGTCAGATCCGTATCAAATTGATAATCAGGATGCAATTGTTTTAATGAGTGTATTCGCGAGCGAATTTCATCATTAATATGCACGGTCTGTGCTTCAGAAGAAATGTCATTTACATCTCCTTTAGTCAATTCAAGTAATTCTTCGACTAATTTTATGATACGATTCATTTCTTCAATAGAAATATTTAACGATTCTTCTAATACTGCTGGGTCTTTTTTTCCCCATCGCTG  >  CP000730/1454506‑1454786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑