Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,469,100	G→A	6.2%	V16V (GTG→GTA)	ypdP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,469,100	0	G	A	6.2%	249.0 / 4.5	81	V16V (GTG→GTA)	ypdP	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/43);  new base A (3/2);  total (36/45)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GCATCGCAAACGATTTTTCAGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACTTTAGGTAATGTCATGTT  >  CP000730/1468960‑1469240                                                                                                                                             |                                                                                                                                             gCATCGCAAACGATTTTTCAGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTg                                                                                                                                              >  1:488951/1‑141 (MQ=255)  cATCGCAAACGATTTTTCAGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGc                                                                                                                                             <  1:427333/141‑1 (MQ=255)        aaaCGATTTTTCAGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATgg                                                                                                                                       >  1:1059046/1‑141 (MQ=255)         aaCGATTTTTCAGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGt                                                                                                                                      <  1:818265/141‑1 (MQ=255)          aCGATTTTTCAGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTa                                                                                                                                     <  2:464951/141‑1 (MQ=255)           cGATTTTTCAGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTAc                                                                                                                                    <  2:22117/141‑1 (MQ=255)             aTTTTTCAGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTCGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACtt                                                                                                                                  <  1:935351/141‑1 (MQ=255)                 ttCAGTAAAATGAGTAAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTACTTATGGTACTTATGt                                                                                                                              <  1:154575/141‑1 (MQ=255)                   cAGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTAt                                                                                                                            <  1:779339/141‑1 (MQ=255)                    aGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATc                                                                                                                           <  1:413180/141‑1 (MQ=255)                    aGTAAAATGAGTAAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTACTTATGGTACTTATGTATc                                                                                                                           >  2:893577/1‑141 (MQ=255)                     gTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCg                                                                                                                          >  2:644629/1‑141 (MQ=255)                      tAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGc                                                                                                                         <  1:669474/141‑1 (MQ=255)                          aTGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCtttt                                                                                                                     >  2:861543/1‑141 (MQ=255)                             agttagttATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTg                                                                                                                  <  1:1061491/141‑1 (MQ=255)                                 agttaTTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTaa                                                                                                              >  2:538382/1‑141 (MQ=255)                                   ttatttatTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAAc                                                                                                            <  2:1041320/141‑1 (MQ=255)                                        tattGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGt                                                                                                       <  2:646532/141‑1 (MQ=255)                                           tGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTa                                                                                                    <  1:14417/141‑1 (MQ=255)                                            gAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGTTATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTaa                                                                                                   <  1:293458/141‑1 (MQ=255)                                               cGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTg                                                                                                <  2:363183/141‑1 (MQ=255)                                                ggTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCTACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGc                                                                                               <  1:246328/141‑1 (MQ=255)                                                  tttCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCAt                                                                                             >  2:1060831/1‑141 (MQ=255)                                                  tttCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCAt                                                                                             >  2:197035/1‑141 (MQ=255)                                                    tCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATgg                                                                                           >  2:490537/1‑141 (MQ=255)                                                    tCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATgg                                                                                           <  2:1049544/141‑1 (MQ=255)                                                     cTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATggg                                                                                          >  1:705013/1‑141 (MQ=255)                                                      tAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGt                                                                                         >  1:349192/1‑141 (MQ=255)                                                      tAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGt                                                                                         <  1:236185/141‑1 (MQ=255)                                                      tAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGt                                                                                         <  1:953571/141‑1 (MQ=255)                                                      tAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGt                                                                                         >  1:442830/1‑141 (MQ=255)                                                       aaaCACCCGCATGATCAAAATTTAGGAGGACATTTTAATATCTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCaacccat                                                                                        <  2:662194/141‑7 (MQ=255)                                                        aaCACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTg                                                                                       >  1:150556/1‑141 (MQ=255)                                                         acacCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGc                                                                                      <  1:366109/141‑1 (MQ=255)                                                          cacCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTATTTGCATGGGTTGCa                                                                                     >  2:282962/1‑141 (MQ=255)                                                            cccGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAAt                                                                                   >  2:699272/1‑141 (MQ=255)                                                               gCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTgg                                                                                >  2:63703/1‑141 (MQ=255)                                                                cATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGc                                                                               >  1:609721/1‑141 (MQ=255)                                                                 aTGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGca                                                                              <  1:121541/141‑1 (MQ=255)                                                                 aTGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGca                                                                              >  1:147896/1‑141 (MQ=255)                                                                   gATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGcaca                                                                            <  2:150556/141‑1 (MQ=255)                                                                     tCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAAt                                                                          <  1:82518/141‑1 (MQ=255)                                                                      cAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATc                                                                         >  1:938187/1‑141 (MQ=255)                                                                       aaaaTTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCa                                                                        >  1:296744/1‑141 (MQ=255)                                                                       aaaaTTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTACTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCa                                                                        >  1:1009883/1‑141 (MQ=255)                                                                        aaaTTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCAt                                                                       >  1:691162/1‑141 (MQ=255)                                                                         aaTTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCAtt                                                                      <  2:265650/141‑1 (MQ=255)                                                                              aggaggACATTTTGATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCaa                                                                 <  2:902911/141‑1 (MQ=255)                                                                              aggaggACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTACTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCaa                                                                 >  1:521837/1‑141 (MQ=255)                                                                                gaggaCATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACa                                                               <  1:174398/141‑1 (MQ=255)                                                                                gaggaCATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACa                                                               >  2:404761/1‑141 (MQ=255)                                                                                     cATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACaa                                                          >  1:48289/1‑141 (MQ=255)                                                                                      aTTTTAATATGTATAATGAAATTTTTGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAg                                                         <  1:130895/141‑1 (MQ=255)                                                                                        tttAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTg                                                       <  1:190038/141‑1 (MQ=255)                                                                                        tttAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTg                                                       <  1:873645/141‑1 (MQ=255)                                                                                         ttAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGa                                                      >  1:640177/1‑141 (MQ=255)                                                                                          tAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGAt                                                     >  2:978777/1‑141 (MQ=255)                                                                                                 tataATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACt                                              >  2:463762/1‑141 (MQ=255)                                                                                                  ataATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTg                                             <  1:50416/141‑1 (MQ=255)                                                                                                    aaTGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTa                                           <  2:60313/141‑1 (MQ=255)                                                                                                    aaTGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTa                                           <  1:905718/141‑1 (MQ=255)                                                                                                     aTGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAg                                          <  1:861543/141‑1 (MQ=255)                                                                                                     aTGAAATTTTAGGACGAGTTACGTTTATTGCAACTTTCGTGCTTATGGTTCTTATGTATCGCTTTTTTGGTAAACAAGGTGTAAATGCATGGGTTGCAATTGGCTCAATCATTGCCAACATACAAGTGATTAAAACTGTAg                                          <  2:795112/141‑1 (MQ=255)                                                                                                        aaaTTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGaaa                                       >  2:741762/1‑141 (MQ=255)                                                                                                           ttttAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCt                                    <  1:810554/141‑1 (MQ=255)                                                                                                           ttttAGGACTAGTTACGTTTATTGCAACGTTCGTACTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCt                                    <  1:893577/141‑1 (MQ=255)                                                                                                            tttAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTACTTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCtt                                   <  2:447032/141‑1 (MQ=255)                                                                                                              tAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTg                                 >  1:180338/1‑141 (MQ=255)                                                                                                                ggACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGt                               >  1:997912/1‑141 (MQ=255)                                                                                                                  aCTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTAt                             >  1:134766/1‑141 (MQ=255)                                                                                                                   cTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTAtt                            <  1:1000318/141‑1 (MQ=255)                                                                                                                   cTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTAtt                            <  1:831275/141‑1 (MQ=255)                                                                                                                     aGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTc                          >  1:1015698/1‑141 (MQ=255)                                                                                                                       ttACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAGCTGTAGAAATCTTTGGTATTTCAg                        <  2:316373/141‑1 (MQ=255)                                                                                                                            tttATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACt                   >  2:205236/1‑141 (MQ=255)                                                                                                                             ttATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACtt                  >  1:131265/1‑141 (MQ=255)                                                                                                                                    aaCGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACTTTAGGTaa           <  1:407258/141‑1 (MQ=255)                                                                                                                                     aCGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACTTTAGGTAAt          >  2:940847/1‑141 (MQ=255)                                                                                                                                      cGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACTTTAGGTAATg         <  2:94893/141‑1 (MQ=255)                                                                                                                                       gTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACTTTAGGTAATGt        <  1:658832/141‑1 (MQ=255)                                                                                                                                         tCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAATTTATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACTTTAGGTAATGTCa      >  1:541854/1‑141 (MQ=255)                                                                                                                                            tGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACTTTAGGTAATGTCATGt   <  2:443741/141‑1 (MQ=255)                                                                                                                                             gCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACTTTAGGTAATGTCATGtt  >  2:316069/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                             GCATCGCAAACGATTTTTCAGTAAAATGAGTTAGTTATTTATTGAGCGGTTTCTAAACACCCGCATGATCAAAATTTAGGAGGACATTTTAATATGTATAATGAAATTTTAGGACTAGTTACGTTTATTGCAACGTTCGTGCTTATGGTACTTATGTATCGCTTTTTTGGTAAACAAGGTTTAATTGCATGGGTTGCAATTGGCACAATCATTGCCAACATACAAGTGATTAAAACTGTAGAAATCTTTGGTATTTCAGCAACTTTAGGTAATGTCATGTT  >  CP000730/1468960‑1469240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑